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The map label for this gene is ydiB [C]

Identifier: 108812181

GI number: 108812181

Start: 2281816

End: 2282634

Strand: Reverse

Name: ydiB [C]

Synonym: YPN_2019

Alternate gene names: 108812181

Gene position: 2282634-2281816 (Counterclockwise)

Preceding gene: 108812182

Following gene: 108812180

Centisome position: 50.34

GC content: 47.99

Gene sequence:

>819_bases
ATGACCCGGTACTTAAACAAAGATACGACAGTGTGTATTTCCCTCGCGGCCAGGCCCAGCAATTTTGGTACCCGTTTTCA
TAACTTTTTGTATGATGCTTTAGATCTCGATTATTTGTATAAAGCTTTTACCACCAACGACTTGGCTGCGGCTATTGGTG
GTGTGCGAGCGTTGGGTTTCCGTGGCTGCGCGATCTCAATGCCGTTTAAAGAGGCTGTTATTCCAATGGTAGATGAGATG
GATCCCTCGGCCAAAGCGATCAACTCAGTGAATACACTGGTTAATACTGGCGGTTATCTGAAAGCATACAATACCGACTA
TATTGCCATTGCCAAATTGTTACAGGAGTATCAGGTCCCGGTAGATAACGTGTTTGCCTTGCGGGGCAGTGGCGGTATGG
CGAAAGCCGTTGCTTTTGCGCTGAAAAATGCTGGCTTCAAACAGGGTTATATTATCGCCACCAATGAAAAGACCGGCAAG
CAACTTGCAGAGCAATCTGGTTACATCTATCGCCCTGATATGCAGAATATTCAGGCTGATATGCTCATTAATGCGACACC
AGTCGGCATGGCTGGGGGGGCTGATGCTGATAAATTGGCTTTTACTGAAACTGAAGTGGACCGCGCGAAAATTGTTTTTG
ATGTTGTGGCGTTGCCCGCCGTCACCCCGCTTATCCGCTATGCTCAGACTCAGGGAAAAACAGTGATCACCGGTGCCGAG
GTTTTTGCTATTCAGGCTGTAGAGCAATTTGTGTTATACACCGGTATCCGCCCAAGTCAGGCGCTGTTTGAACAAGCCGC
AGCTTATGCAAGAGGCTGA

Upstream 100 bases:

>100_bases
GTCCATAATTTTTACCACAACAAGCATTTCATCGTCTGTTGTGCTCTCGCGCTACAATGGGAGCCAGTGGGCGGACGCGA
TTGACATCAAGGTGAAGAAG

Downstream 100 bases:

>100_bases
TTTATAAGCGCAGTCCGCGATCCTTAATGTCAGTGAGAAAGGTTAGTCAGCCGGGGTGTTTGGGCTGGTTGTGTGTCATT
CGCCCTGGAAAATGTCTAAT

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTRYLNKDTTVCISLAARPSNFGTRFHNFLYDALDLDYLYKAFTTNDLAAAIGGVRALGFRGCAISMPFKEAVIPMVDEM
DPSAKAINSVNTLVNTGGYLKAYNTDYIAIAKLLQEYQVPVDNVFALRGSGGMAKAVAFALKNAGFKQGYIIATNEKTGK
QLAEQSGYIYRPDMQNIQADMLINATPVGMAGGADADKLAFTETEVDRAKIVFDVVALPAVTPLIRYAQTQGKTVITGAE
VFAIQAVEQFVLYTGIRPSQALFEQAAAYARG

Sequences:

>Translated_272_residues
MTRYLNKDTTVCISLAARPSNFGTRFHNFLYDALDLDYLYKAFTTNDLAAAIGGVRALGFRGCAISMPFKEAVIPMVDEM
DPSAKAINSVNTLVNTGGYLKAYNTDYIAIAKLLQEYQVPVDNVFALRGSGGMAKAVAFALKNAGFKQGYIIATNEKTGK
QLAEQSGYIYRPDMQNIQADMLINATPVGMAGGADADKLAFTETEVDRAKIVFDVVALPAVTPLIRYAQTQGKTVITGAE
VFAIQAVEQFVLYTGIRPSQALFEQAAAYARG
>Mature_271_residues
TRYLNKDTTVCISLAARPSNFGTRFHNFLYDALDLDYLYKAFTTNDLAAAIGGVRALGFRGCAISMPFKEAVIPMVDEMD
PSAKAINSVNTLVNTGGYLKAYNTDYIAIAKLLQEYQVPVDNVFALRGSGGMAKAVAFALKNAGFKQGYIIATNEKTGKQ
LAEQSGYIYRPDMQNIQADMLINATPVGMAGGADADKLAFTETEVDRAKIVFDVVALPAVTPLIRYAQTQGKTVITGAEV
FAIQAVEQFVLYTGIRPSQALFEQAAAYARG

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=268, Percent_Identity=29.1044776119403, Blast_Score=92, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320332, Length=262, Percent_Identity=25.9541984732824, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 29419; Mature: 29288

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYLNKDTTVCISLAARPSNFGTRFHNFLYDALDLDYLYKAFTTNDLAAAIGGVRALGF
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
RGCAISMPFKEAVIPMVDEMDPSAKAINSVNTLVNTGGYLKAYNTDYIAIAKLLQEYQVP
CCEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCC
VDNVFALRGSGGMAKAVAFALKNAGFKQGYIIATNEKTGKQLAEQSGYIYRPDMQNIQAD
HHHEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCEECCCHHCCCCC
MLINATPVGMAGGADADKLAFTETEVDRAKIVFDVVALPAVTPLIRYAQTQGKTVITGAE
EEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHH
VFAIQAVEQFVLYTGIRPSQALFEQAAAYARG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRYLNKDTTVCISLAARPSNFGTRFHNFLYDALDLDYLYKAFTTNDLAAAIGGVRALGF
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
RGCAISMPFKEAVIPMVDEMDPSAKAINSVNTLVNTGGYLKAYNTDYIAIAKLLQEYQVP
CCEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCC
VDNVFALRGSGGMAKAVAFALKNAGFKQGYIIATNEKTGKQLAEQSGYIYRPDMQNIQAD
HHHEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCEECCCHHCCCCC
MLINATPVGMAGGADADKLAFTETEVDRAKIVFDVVALPAVTPLIRYAQTQGKTVITGAE
EEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHH
VFAIQAVEQFVLYTGIRPSQALFEQAAAYARG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]