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The map label for this gene is acrA [H]

Identifier: 108811123

GI number: 108811123

Start: 1113440

End: 1114627

Strand: Reverse

Name: acrA [H]

Synonym: YPN_0958

Alternate gene names: 108811123

Gene position: 1114627-1113440 (Counterclockwise)

Preceding gene: 108811127

Following gene: 108811122

Centisome position: 24.58

GC content: 49.07

Gene sequence:

>1188_bases
ATGAGCAAAAACAGAGGGGTAATGCCTCTGGCTGCAATTCTGGTACTTTCAGGCAGCTTAGTACTTATAGGATGTAATGA
CAAAGATGCGGCGCAAGCCGGTGCTCAACAAGCTCCTGAGGTTGGTGTTGTGACATTGAAAGCAGAACCGCTGAATATCA
CAACCGATTTGCCAGGCCGCACGTCCGCCTTCCGTGTTGCAGAAGTTCGCCCGCAGGTAAGCGGGATTATTCTCAAACGT
AATTATATTGAAGGCAGTGATGTCACCGCCGGAACATCCCTTTATCAAATTGACCCTGCGACTTATCAGGCCGCTTATGA
CAGTGCAAAAGGTGATCTGGCTAAAGCACAGGCTTCAGCACAAATTGCTCATTTAACCGTCAATCGCTACAAGCCATTGT
TGGGTACTAACTACATCAGTAAACAAGAATATGATCAGGCCCTATCAGATGCCCAACAAGCAGATGCCACCGTGTTAGCA
GCAAAAGCGGCACTGGAGTCTGCACGCATTAATCTGGCGTATACCCAAGTGAGATCACCAATCAGCGGCCGTACCGGTAA
ATCTGCCGTGACGGAAGGTGCATTGGTTACCAGTGGTCAGGCGTCAGCAATGACTACCGTTCAACAGCTTGATCCAATGT
ATGTCGATGTCACTCAATCGAGTGATGAGTTCCTACGGCTGAAAAAAGAGCTGGCTGATGGCATTCTCAAGCAAGAAAAC
GGCAAAGCTAAAGTCCGTTTGTTGCTGGAAAACGGGGTTGAATATACAGAAACCGGTACTTTGGAGTTCTCCGGTGTCAC
TGTTGATGAAACTACCGGTTCGATTACTCTCCGTGCGATCTTCCCTAATCCAAATGAGGCGCTGTTGCCAGGCATGTTTG
TCCGTGCCCGTTTGGATGAAGGTATCCGTCCTGATGCGCTGTTAGTTCCGCAACAAGGCGTGACTCGCAACCCACGTGGT
GAAGCAACAGCCATGGTCGTCGGCGCGGGTAATAAAGTTGAAATGCGTACCCTGATAGCGAACCAAGCAATCGGTAATAA
ATGGCTGGTCACTGAAGGGCTAAAAGCCGGTGATCGCCTGATTATTTCCGGCTTGCAGAAAATCAAACCAGGTGTAGAGG
TAAAAGTGCAGGAAGTTACTGACACTGCACCAGAAACAGCCCCGGCTGATACAGCGAAGAAGTCTTAA

Upstream 100 bases:

>100_bases
TGTATGTACCATAGCACGCACATATCATTTACGCAGCAATGGGTTTTAAAGCTTGTGATCCATTGAATTAAATTAAAAAT
CGGACACTTGAGGTTTATCT

Downstream 100 bases:

>100_bases
AAGGAGCCGGTAATTCATGGCTAAGTTCTTTATAGATCGCCCTATTTTCGCTTGGGTCATCGCCATCATCATCATGTTGG
CTGGTGCCCTTGCGATCATG

Product: multidrug efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSKNRGVMPLAAILVLSGSLVLIGCNDKDAAQAGAQQAPEVGVVTLKAEPLNITTDLPGRTSAFRVAEVRPQVSGIILKR
NYIEGSDVTAGTSLYQIDPATYQAAYDSAKGDLAKAQASAQIAHLTVNRYKPLLGTNYISKQEYDQALSDAQQADATVLA
AKAALESARINLAYTQVRSPISGRTGKSAVTEGALVTSGQASAMTTVQQLDPMYVDVTQSSDEFLRLKKELADGILKQEN
GKAKVRLLLENGVEYTETGTLEFSGVTVDETTGSITLRAIFPNPNEALLPGMFVRARLDEGIRPDALLVPQQGVTRNPRG
EATAMVVGAGNKVEMRTLIANQAIGNKWLVTEGLKAGDRLIISGLQKIKPGVEVKVQEVTDTAPETAPADTAKKS

Sequences:

>Translated_395_residues
MSKNRGVMPLAAILVLSGSLVLIGCNDKDAAQAGAQQAPEVGVVTLKAEPLNITTDLPGRTSAFRVAEVRPQVSGIILKR
NYIEGSDVTAGTSLYQIDPATYQAAYDSAKGDLAKAQASAQIAHLTVNRYKPLLGTNYISKQEYDQALSDAQQADATVLA
AKAALESARINLAYTQVRSPISGRTGKSAVTEGALVTSGQASAMTTVQQLDPMYVDVTQSSDEFLRLKKELADGILKQEN
GKAKVRLLLENGVEYTETGTLEFSGVTVDETTGSITLRAIFPNPNEALLPGMFVRARLDEGIRPDALLVPQQGVTRNPRG
EATAMVVGAGNKVEMRTLIANQAIGNKWLVTEGLKAGDRLIISGLQKIKPGVEVKVQEVTDTAPETAPADTAKKS
>Mature_394_residues
SKNRGVMPLAAILVLSGSLVLIGCNDKDAAQAGAQQAPEVGVVTLKAEPLNITTDLPGRTSAFRVAEVRPQVSGIILKRN
YIEGSDVTAGTSLYQIDPATYQAAYDSAKGDLAKAQASAQIAHLTVNRYKPLLGTNYISKQEYDQALSDAQQADATVLAA
KAALESARINLAYTQVRSPISGRTGKSAVTEGALVTSGQASAMTTVQQLDPMYVDVTQSSDEFLRLKKELADGILKQENG
KAKVRLLLENGVEYTETGTLEFSGVTVDETTGSITLRAIFPNPNEALLPGMFVRARLDEGIRPDALLVPQQGVTRNPRGE
ATAMVVGAGNKVEMRTLIANQAIGNKWLVTEGLKAGDRLIISGLQKIKPGVEVKVQEVTDTAPETAPADTAKKS

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=380, Percent_Identity=75.5263157894737, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1789665, Length=394, Percent_Identity=62.1827411167513, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI1789929, Length=362, Percent_Identity=56.353591160221, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI87082036, Length=382, Percent_Identity=28.5340314136126, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081951, Length=125, Percent_Identity=38.4, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41890; Mature: 41759

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNRGVMPLAAILVLSGSLVLIGCNDKDAAQAGAQQAPEVGVVTLKAEPLNITTDLPGR
CCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCCCCEEEEEECCEEEEECCCCC
TSAFRVAEVRPQVSGIILKRNYIEGSDVTAGTSLYQIDPATYQAAYDSAKGDLAKAQASA
CCEEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCHHHHHHCC
QIAHLTVNRYKPLLGTNYISKQEYDQALSDAQQADATVLAAKAALESARINLAYTQVRSP
EEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEHHHCCC
ISGRTGKSAVTEGALVTSGQASAMTTVQQLDPMYVDVTQSSDEFLRLKKELADGILKQEN
CCCCCCCHHHCCCCEEECCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
GKAKVRLLLENGVEYTETGTLEFSGVTVDETTGSITLRAIFPNPNEALLPGMFVRARLDE
CCEEEEEEEECCCCEEECCEEEEECEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHCC
GIRPDALLVPQQGVTRNPRGEATAMVVGAGNKVEMRTLIANQAIGNKWLVTEGLKAGDRL
CCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCEEEEECCCCCCCHH
IISGLQKIKPGVEVKVQEVTDTAPETAPADTAKKS
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKNRGVMPLAAILVLSGSLVLIGCNDKDAAQAGAQQAPEVGVVTLKAEPLNITTDLPGR
CCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCCCCEEEEEECCEEEEECCCCC
TSAFRVAEVRPQVSGIILKRNYIEGSDVTAGTSLYQIDPATYQAAYDSAKGDLAKAQASA
CCEEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCHHHHHHCC
QIAHLTVNRYKPLLGTNYISKQEYDQALSDAQQADATVLAAKAALESARINLAYTQVRSP
EEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEHHHCCC
ISGRTGKSAVTEGALVTSGQASAMTTVQQLDPMYVDVTQSSDEFLRLKKELADGILKQEN
CCCCCCCHHHCCCCEEECCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
GKAKVRLLLENGVEYTETGTLEFSGVTVDETTGSITLRAIFPNPNEALLPGMFVRARLDE
CCEEEEEEEECCCCEEECCEEEEECEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHCC
GIRPDALLVPQQGVTRNPRGEATAMVVGAGNKVEMRTLIANQAIGNKWLVTEGLKAGDRL
CCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCEEEEECCCCCCCHH
IISGLQKIKPGVEVKVQEVTDTAPETAPADTAKKS
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]