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The map label for this gene is paaF [C]

Identifier: 108797951

GI number: 108797951

Start: 1086294

End: 1087058

Strand: Direct

Name: paaF [C]

Synonym: Mmcs_0978

Alternate gene names: 108797951

Gene position: 1086294-1087058 (Clockwise)

Preceding gene: 108797950

Following gene: 108797954

Centisome position: 19.04

GC content: 73.73

Gene sequence:

>765_bases
ATGACAGGACCCGTCCGCGTCGAACGCAACGGCGCGGTGACCACCGTGATCATCGACCGGCCCGACGCCCGCAACGCCGT
CAACCGCCCCACCGCCGAGGCGCTCTACGAGGCCTTCGACGCATTCGACCGCGACGACTCCGCAGCGGTCGCCGTGCTGT
GGGGTGACCACGGCACCTTCTGCGCCGGTGCCGATCTCAAGGCGTTCGGCACCCCCGACGCCAACGCCGTGCACCGCAGC
GGACCCGGCCCGATGGGGCCCACCCGCATGGTGCTGTCGAAACCGGTGATCGCCGCGGTCAGCGGATACGCCGTGGCGGG
CGGACTCGAACTCGCGCTGTGGTGCGATCTGCGCGTCGTGGAGGAGGACGCGGTGCTCGGGGTGTTCTGCCGACGCTGGG
GGGTGCCGCTGATCGACGGCGGCACCGTGCGGCTGCCCCGGCTGATCGGGCAGAGCCGGGCGATGGACCTGATCCTGACT
GGCCGGCCCGTCGACGCCGCCGAGGCCCACGCGATCGGCCTGGCCAACCGCGTCGTCCCGAAAGGTCAGGCGCGTCGACA
GGCCGAGGAGCTGGCCGCCGAGATCGCCCGGTTCCCGCAGCAGTGCATGCGCGCCGACCGCGGTTCGGCGCTGGGCCAGT
GGGGGCTGCCGGAGGCCGAGGCGATCGACCGGGAGTTCGCCAGCATCTCGCGGGTCAGGGACGAGGCGCTCGAGGGCGCG
GGCCGCTTCGCCGCCGGGGCGGGCCGCCACGGCGCCAAAGCGTGA

Upstream 100 bases:

>100_bases
GGCCCGGCGACGAATTACGCACCGCCTACACCGCTTTCGCCGCCGAACTCGTCGAGCGGCGCGATGCCCAACTCAGAGAC
GCCCAACTCATGGAGGCGAC

Downstream 100 bases:

>100_bases
TTTCGGCGTGCGCGGTCGCGCCCAGCGCGACCCAGCACGCCGAAATCACCCCTGCTTGGTGATCTTGTTGTTCGAGCCGA
GGTCGTCGATCTTGGGGTCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTGPVRVERNGAVTTVIIDRPDARNAVNRPTAEALYEAFDAFDRDDSAAVAVLWGDHGTFCAGADLKAFGTPDANAVHRS
GPGPMGPTRMVLSKPVIAAVSGYAVAGGLELALWCDLRVVEEDAVLGVFCRRWGVPLIDGGTVRLPRLIGQSRAMDLILT
GRPVDAAEAHAIGLANRVVPKGQARRQAEELAAEIARFPQQCMRADRGSALGQWGLPEAEAIDREFASISRVRDEALEGA
GRFAAGAGRHGAKA

Sequences:

>Translated_254_residues
MTGPVRVERNGAVTTVIIDRPDARNAVNRPTAEALYEAFDAFDRDDSAAVAVLWGDHGTFCAGADLKAFGTPDANAVHRS
GPGPMGPTRMVLSKPVIAAVSGYAVAGGLELALWCDLRVVEEDAVLGVFCRRWGVPLIDGGTVRLPRLIGQSRAMDLILT
GRPVDAAEAHAIGLANRVVPKGQARRQAEELAAEIARFPQQCMRADRGSALGQWGLPEAEAIDREFASISRVRDEALEGA
GRFAAGAGRHGAKA
>Mature_253_residues
TGPVRVERNGAVTTVIIDRPDARNAVNRPTAEALYEAFDAFDRDDSAAVAVLWGDHGTFCAGADLKAFGTPDANAVHRSG
PGPMGPTRMVLSKPVIAAVSGYAVAGGLELALWCDLRVVEEDAVLGVFCRRWGVPLIDGGTVRLPRLIGQSRAMDLILTG
RPVDAAEAHAIGLANRVVPKGQARRQAEELAAEIARFPQQCMRADRGSALGQWGLPEAEAIDREFASISRVRDEALEGAG
RFAAGAGRHGAKA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=237, Percent_Identity=29.957805907173, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4502327, Length=205, Percent_Identity=35.609756097561, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI20127408, Length=194, Percent_Identity=30.4123711340206, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI68989263, Length=155, Percent_Identity=34.1935483870968, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=32.2709163346614, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI221142681, Length=201, Percent_Identity=36.81592039801, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788597, Length=226, Percent_Identity=28.7610619469027, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788682, Length=142, Percent_Identity=41.5492957746479, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790281, Length=172, Percent_Identity=30.2325581395349, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787660, Length=199, Percent_Identity=33.6683417085427, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=242, Percent_Identity=46.6942148760331, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=31.8367346938775, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=30.8370044052863, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17558304, Length=209, Percent_Identity=33.0143540669856, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=203, Percent_Identity=34.9753694581281, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=187, Percent_Identity=35.8288770053476, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17508951, Length=183, Percent_Identity=36.0655737704918, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=188, Percent_Identity=31.9148936170213, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=194, Percent_Identity=36.0824742268041, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=201, Percent_Identity=27.8606965174129, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=244, Percent_Identity=25.4098360655738, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357171, Length=209, Percent_Identity=50.2392344497608, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24650670, Length=211, Percent_Identity=47.8672985781991, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24653139, Length=212, Percent_Identity=36.7924528301887, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI20129971, Length=191, Percent_Identity=34.5549738219895, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653477, Length=191, Percent_Identity=34.5549738219895, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583077, Length=198, Percent_Identity=32.3232323232323, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24583079, Length=198, Percent_Identity=32.3232323232323, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19921000, Length=196, Percent_Identity=31.6326530612245, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26844; Mature: 26713

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPVRVERNGAVTTVIIDRPDARNAVNRPTAEALYEAFDAFDRDDSAAVAVLWGDHGTF
CCCCEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCE
CAGADLKAFGTPDANAVHRSGPGPMGPTRMVLSKPVIAAVSGYAVAGGLELALWCDLRVV
EECCCCEECCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHCCEEEECCCEEEEEEEEEEE
EEDAVLGVFCRRWGVPLIDGGTVRLPRLIGQSRAMDLILTGRPVDAAEAHAIGLANRVVP
CCHHHHHHHHHHCCCCEECCCCEEHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
KGQARRQAEELAAEIARFPQQCMRADRGSALGQWGLPEAEAIDREFASISRVRDEALEGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GRFAAGAGRHGAKA
CCCCCCCCCCCCCC
>Mature Secondary Structure 
TGPVRVERNGAVTTVIIDRPDARNAVNRPTAEALYEAFDAFDRDDSAAVAVLWGDHGTF
CCCEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCE
CAGADLKAFGTPDANAVHRSGPGPMGPTRMVLSKPVIAAVSGYAVAGGLELALWCDLRVV
EECCCCEECCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHCCEEEECCCEEEEEEEEEEE
EEDAVLGVFCRRWGVPLIDGGTVRLPRLIGQSRAMDLILTGRPVDAAEAHAIGLANRVVP
CCHHHHHHHHHHCCCCEECCCCEEHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
KGQARRQAEELAAEIARFPQQCMRADRGSALGQWGLPEAEAIDREFASISRVRDEALEGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GRFAAGAGRHGAKA
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA