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The map label for this gene is thiE [H]

Identifier: 108797524

GI number: 108797524

Start: 603421

End: 604101

Strand: Reverse

Name: thiE [H]

Synonym: Mmcs_0544

Alternate gene names: 108797524

Gene position: 604101-603421 (Counterclockwise)

Preceding gene: 108797531

Following gene: 108797522

Centisome position: 10.59

GC content: 73.27

Gene sequence:

>681_bases
GTGCGTGAACCCCTCGACCCGCTCGGCCCAGCCCTTGCGCAAGCCTCGCTGTACCTGTGCACAGACGCCCGCCGCGAACG
CGGCGACCTCGCCGAATTCGCCGACGCGGCGCTCGCGGGCGGCGTCGACCTGATCCAGCTGCGGGACAAGGGGTCGGCAG
GCGAACGACGGTTCGGCCCACTCGAGGCGCGCGAGGAACTGGCCGCGCTCGAAATCCTCGCCGAAGCCGCCCGCCGCCAC
GGCGCACTGTTGGCGGTCAACGACCGCGCCGATATCGCGCTGGCCGCCGGCGCCGACGTCCTGCACCTCGGTCAGGACGA
CCTGCCGCTGCCCGTGGCCCGCCGGATCATCGGTCCAAGCCCGCTGATCGGGCGGTCCACCCACGACAGCGCCCAGGTCG
CCGCGGCAGTGGCCGAGGAGGTCGACTACTTCTGCGTCGGGCCGTGCTGGCCGACGCCGACCAAACCCGGACGCGAGGCC
CCCGGGCTCGGCCTGGTTCGCGAGGTCGCTTCGCGGGCAACAGAGAAACCGTGGTTCGCCATCGGCGGTATCGACGAGGC
GCGGCTGCCCGAGGTGCTCGACGCCGGCGCCCGCCGCATCGTCGTGGTCCGCGCGATCACGGCGGCCGACGATCCGAAGG
CGGCGGCTCGCCGGCTGAAGGATGCGCTCGTCAGTCGCTGA

Upstream 100 bases:

>100_bases
GGTAAGGGCCGGATCGTCTGGCCCACTCTCAGTCCCGCTGTCCCGGGACTCCCGTGTCGGCCATCAACCCTACCCGCGGC
CGAGGCGCTACCGTCGCCGT

Downstream 100 bases:

>100_bases
CCAGCAGCGGAAAAGCCGCGGTGACGGTCCGCAGTCGGTCCCAGCTCGCGGCGAAACCCGGGTGCAGCGGCAGGGAGTCG
ACGTCCTCCTCGGCGACCCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MREPLDPLGPALAQASLYLCTDARRERGDLAEFADAALAGGVDLIQLRDKGSAGERRFGPLEAREELAALEILAEAARRH
GALLAVNDRADIALAAGADVLHLGQDDLPLPVARRIIGPSPLIGRSTHDSAQVAAAVAEEVDYFCVGPCWPTPTKPGREA
PGLGLVREVASRATEKPWFAIGGIDEARLPEVLDAGARRIVVVRAITAADDPKAAARRLKDALVSR

Sequences:

>Translated_226_residues
MREPLDPLGPALAQASLYLCTDARRERGDLAEFADAALAGGVDLIQLRDKGSAGERRFGPLEAREELAALEILAEAARRH
GALLAVNDRADIALAAGADVLHLGQDDLPLPVARRIIGPSPLIGRSTHDSAQVAAAVAEEVDYFCVGPCWPTPTKPGREA
PGLGLVREVASRATEKPWFAIGGIDEARLPEVLDAGARRIVVVRAITAADDPKAAARRLKDALVSR
>Mature_226_residues
MREPLDPLGPALAQASLYLCTDARRERGDLAEFADAALAGGVDLIQLRDKGSAGERRFGPLEAREELAALEILAEAARRH
GALLAVNDRADIALAAGADVLHLGQDDLPLPVARRIIGPSPLIGRSTHDSAQVAAAVAEEVDYFCVGPCWPTPTKPGREA
PGLGLVREVASRATEKPWFAIGGIDEARLPEVLDAGARRIVVVRAITAADDPKAAARRLKDALVSR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=192, Percent_Identity=35.4166666666667, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=229, Percent_Identity=32.3144104803493, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23895; Mature: 23895

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREPLDPLGPALAQASLYLCTDARRERGDLAEFADAALAGGVDLIQLRDKGSAGERRFGP
CCCCCCHHHHHHHHHHHHEECCCHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
LEAREELAALEILAEAARRHGALLAVNDRADIALAAGADVLHLGQDDLPLPVARRIIGPS
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCEEECCCCCCCHHHHHHHCCCC
PLIGRSTHDSAQVAAAVAEEVDYFCVGPCWPTPTKPGREAPGLGLVREVASRATEKPWFA
CCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
IGGIDEARLPEVLDAGARRIVVVRAITAADDPKAAARRLKDALVSR
ECCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MREPLDPLGPALAQASLYLCTDARRERGDLAEFADAALAGGVDLIQLRDKGSAGERRFGP
CCCCCCHHHHHHHHHHHHEECCCHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
LEAREELAALEILAEAARRHGALLAVNDRADIALAAGADVLHLGQDDLPLPVARRIIGPS
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCEEECCCCCCCHHHHHHHCCCC
PLIGRSTHDSAQVAAAVAEEVDYFCVGPCWPTPTKPGREAPGLGLVREVASRATEKPWFA
CCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
IGGIDEARLPEVLDAGARRIVVVRAITAADDPKAAARRLKDALVSR
ECCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA