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The map label for this gene is hsaD [H]

Identifier: 108797303

GI number: 108797303

Start: 361723

End: 362580

Strand: Reverse

Name: hsaD [H]

Synonym: Mmcs_0323

Alternate gene names: 108797303

Gene position: 362580-361723 (Counterclockwise)

Preceding gene: 108797304

Following gene: 108797299

Centisome position: 6.35

GC content: 67.37

Gene sequence:

>858_bases
ATGACCGCGACGTCCGACCAGTTGCCGCTGCAGGAACTGACCACGCCGTCGGGCACCCTGCGGTACTACGACACCGGGGC
CGGGCCGACGGTGCTGTTCCTGCACGGTTCCGGCCCGGGCGTCACGGGATGGCGAAACTTCCGCGGTGTCCTGCCTGCGT
TCAGCGCGCACTTCCGTTGTCTCATCCTCGAATTCCCCGGATTCGGGGTCAGTGACGATGCGGGCGGTCATCCGATGATC
GACGCGCAGGGGGCGGTGGCGCCGTTCCTCGACGGCCTCGGGGTGGACCGCGTGGACATCGTCGGCAACTCGATGGGCGG
CGGTGTCGGGATCAACTTCGCCATCCACCACCCCGACCGCATCCGCAGGCTGGTGACCATCGGCGGTATCGGCACCAACG
TCTTCAGCCCGGGCCCGAGTGAGGGCATCCGGCTGCTGCAGGAGTTCACCGAGGACCCCACCCGTGCGCGGTTGGTCGAC
TGGCTGCGGTCGATGGTCTACGACGAGGCGCTGGTCACCGAGGAGCTGATCGCCGAGCGATGGGAGCTGGCAACGGATCC
CGAGACGCTCGCCGCTGCGCGCCGCATGTACGGCAAAGCCGCGTTCACGCAGATGATGGCGTTCATGCGCAAGTCGGAAG
CCCCACTGCCGTGGGCGCAGATGCACAGAGTCGCTGCGCCGACGTTGCTCACCTGGGGCCGCGACGACCGCGTCAGCCCA
CTGGACATGGCGCTGATCCCGATGCGGACGATCCCCAACGCGGAGCTGCACGTGTTCCCGAACTGCGGACACTGGGCGAT
GATCGAGGCCAAGGCGGCGTTCGAAGCCGCGGTATTGTCATTCCTGTTGCGAGAGTGA

Upstream 100 bases:

>100_bases
ATCGCGGTGATCGCCCGACACGATGCCGAGGAAAGCCTCCCCAGCGCGGGCCTTCGGGCAGTCTGAGCGCCGACCCGTCA
CACCAAACGAGGAGCCGTAC

Downstream 100 bases:

>100_bases
CGCTCTCAGCGTTGCGTGCTGCCCGCGTCAACGGGTAGCGACACCGCCGTGATGTAGCGAGCTTCGTCGGACGCCAGGAA
CAGCGAGGCGTTGGCCACGT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; Meta-cleavage product hydrolase; MCP hydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTATSDQLPLQELTTPSGTLRYYDTGAGPTVLFLHGSGPGVTGWRNFRGVLPAFSAHFRCLILEFPGFGVSDDAGGHPMI
DAQGAVAPFLDGLGVDRVDIVGNSMGGGVGINFAIHHPDRIRRLVTIGGIGTNVFSPGPSEGIRLLQEFTEDPTRARLVD
WLRSMVYDEALVTEELIAERWELATDPETLAAARRMYGKAAFTQMMAFMRKSEAPLPWAQMHRVAAPTLLTWGRDDRVSP
LDMALIPMRTIPNAELHVFPNCGHWAMIEAKAAFEAAVLSFLLRE

Sequences:

>Translated_285_residues
MTATSDQLPLQELTTPSGTLRYYDTGAGPTVLFLHGSGPGVTGWRNFRGVLPAFSAHFRCLILEFPGFGVSDDAGGHPMI
DAQGAVAPFLDGLGVDRVDIVGNSMGGGVGINFAIHHPDRIRRLVTIGGIGTNVFSPGPSEGIRLLQEFTEDPTRARLVD
WLRSMVYDEALVTEELIAERWELATDPETLAAARRMYGKAAFTQMMAFMRKSEAPLPWAQMHRVAAPTLLTWGRDDRVSP
LDMALIPMRTIPNAELHVFPNCGHWAMIEAKAAFEAAVLSFLLRE
>Mature_284_residues
TATSDQLPLQELTTPSGTLRYYDTGAGPTVLFLHGSGPGVTGWRNFRGVLPAFSAHFRCLILEFPGFGVSDDAGGHPMID
AQGAVAPFLDGLGVDRVDIVGNSMGGGVGINFAIHHPDRIRRLVTIGGIGTNVFSPGPSEGIRLLQEFTEDPTRARLVDW
LRSMVYDEALVTEELIAERWELATDPETLAAARRMYGKAAFTQMMAFMRKSEAPLPWAQMHRVAAPTLLTWGRDDRVSPL
DMALIPMRTIPNAELHVFPNCGHWAMIEAKAAFEAAVLSFLLRE

Specific function: Catalyzes the hydrolysis of a carbon-carbon bond in 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19- hexanorandrostan-5-oic acid (DOHNAA) and 2-hydroxy-hexa-2,4- dienoic acid (HHD). Is

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. HsaD family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=291, Percent_Identity=34.7079037800687, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.7.1.8 [H]

Molecular weight: Translated: 31145; Mature: 31014

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATSDQLPLQELTTPSGTLRYYDTGAGPTVLFLHGSGPGVTGWRNFRGVLPAFSAHFRC
CCCCCCCCCHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEE
LILEFPGFGVSDDAGGHPMIDAQGAVAPFLDGLGVDRVDIVGNSMGGGVGINFAIHHPDR
EEEECCCCCCCCCCCCCCEECCCCCHHHHHHCCCCCEEEEECCCCCCCEEEEEEEECHHH
IRRLVTIGGIGTNVFSPGPSEGIRLLQEFTEDPTRARLVDWLRSMVYDEALVTEELIAER
HHEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WELATDPETLAAARRMYGKAAFTQMMAFMRKSEAPLPWAQMHRVAAPTLLTWGRDDRVSP
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCCCCCCCC
LDMALIPMRTIPNAELHVFPNCGHWAMIEAKAAFEAAVLSFLLRE
CCEEEECCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TATSDQLPLQELTTPSGTLRYYDTGAGPTVLFLHGSGPGVTGWRNFRGVLPAFSAHFRC
CCCCCCCCHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEE
LILEFPGFGVSDDAGGHPMIDAQGAVAPFLDGLGVDRVDIVGNSMGGGVGINFAIHHPDR
EEEECCCCCCCCCCCCCCEECCCCCHHHHHHCCCCCEEEEECCCCCCCEEEEEEEECHHH
IRRLVTIGGIGTNVFSPGPSEGIRLLQEFTEDPTRARLVDWLRSMVYDEALVTEELIAER
HHEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WELATDPETLAAARRMYGKAAFTQMMAFMRKSEAPLPWAQMHRVAAPTLLTWGRDDRVSP
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCCCCCCCC
LDMALIPMRTIPNAELHVFPNCGHWAMIEAKAAFEAAVLSFLLRE
CCEEEECCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]