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The map label for this gene is kshB [H]

Identifier: 108797299

GI number: 108797299

Start: 357328

End: 358368

Strand: Reverse

Name: kshB [H]

Synonym: Mmcs_0319

Alternate gene names: 108797299

Gene position: 358368-357328 (Counterclockwise)

Preceding gene: 108797303

Following gene: 108797298

Centisome position: 6.28

GC content: 70.22

Gene sequence:

>1041_bases
ATGATCGCCGACGTGGATTCGCCATCTGAGCCTCGCACCGTGCGGCTCACCGTCACCGACGTCATCGAGGAGAGCCACGA
TGCCCGGTCGCTGGTCTTCGCGGTCGCCGACGGCGCCCGCTTCGACTACCGGCCCGGCCAATTCTTGACATTGCGGGTGC
CCGGCGAGCAGGGCGGCGCGGTGGCCCGCTGCTATTCGCTGGCCAGCTCCCCGCACACCGACGCCGCACCCAAGGTGACG
ATCAAACGCGTCGACGGCGGATACGGGTCGAACTGGTTGTGCGACAACGTGGTCGCCGGCCAGGTGATTGAGGCGCTGCC
GCCTTCGGGAGTGTTCACCCCCGGCGACCTCGACCGCGACCTGGTGCTGTGGGCCGGCGGCAGCGGCATCACGCCGGTCA
TGTCGATCCTGAAGTCGGCGCTCGCGGTGGGCAGCGGACGGGTTGTGCTCGTCTACGCCAATCGCGACGAGCGGTCGGTG
ATCTTCGCCGCCGAACTGCGCGACCTGACCGCCCGCCACCCCGACCGACTGACCGTGGTGCACTGGCTGGAGTCCGTGCA
GGGACTGCCCCGGCAGGACCAGATTGCGACCCTGGCACGACGATTCACCGGCGGTGAGGCGTACCTCTGCGGGCCGGCAC
CGTTCATGGCCGCGGTGCAGGCGGCGCTGGCCGAAGCGGGCGTACCCCGCGACGCCGTGCACACCGAGGTGTTCCGGTCG
TTGACCGGTGACCCGTTCGCCGAGGTTGCGCCGGTCCCAGTTGACGGGGACGCCGCCGACCTGCAGGTCGAGCTCGACGG
CGGCAAGCACAGCCTTCGGTGGCCCCGCCAGGCCACCCTCGTCGACGCGATGATCGCGGCGGGCCTCGACGTGCCGTACT
CCTGCCGCGAGGGGCAGTGCGGATCGTGTGCCGCCACCCTGCTGCGCGGCCGGGTGGACATGCCCGACACCGAGATCCTC
GAACCAGAGGACATCGAGGCCGGCACGATCCTCGGCTGTCAGGCGCGTCCGGCGTCCGACGAGATCCACATCGAGTTCTG
A

Upstream 100 bases:

>100_bases
AGGTGTCCACGACGATCAAGCTAGGCGTGGGCCGCCCGCGCGCGGAGGGGTCCGTCCCGGTCAACGGACGTCGCGTTCCT
GTCGGGCACAGTCCGCGCCC

Downstream 100 bases:

>100_bases
CGGTCTTTCCATTCACCGGGATTCGCCCACGAGCGACGCTCGATACCGGTAGACCATCGAAGAAGACTCACGAGAGAAGG
ACGCCTCATGACCGAGCGGG

Product: ferredoxin

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MIADVDSPSEPRTVRLTVTDVIEESHDARSLVFAVADGARFDYRPGQFLTLRVPGEQGGAVARCYSLASSPHTDAAPKVT
IKRVDGGYGSNWLCDNVVAGQVIEALPPSGVFTPGDLDRDLVLWAGGSGITPVMSILKSALAVGSGRVVLVYANRDERSV
IFAAELRDLTARHPDRLTVVHWLESVQGLPRQDQIATLARRFTGGEAYLCGPAPFMAAVQAALAEAGVPRDAVHTEVFRS
LTGDPFAEVAPVPVDGDAADLQVELDGGKHSLRWPRQATLVDAMIAAGLDVPYSCREGQCGSCAATLLRGRVDMPDTEIL
EPEDIEAGTILGCQARPASDEIHIEF

Sequences:

>Translated_346_residues
MIADVDSPSEPRTVRLTVTDVIEESHDARSLVFAVADGARFDYRPGQFLTLRVPGEQGGAVARCYSLASSPHTDAAPKVT
IKRVDGGYGSNWLCDNVVAGQVIEALPPSGVFTPGDLDRDLVLWAGGSGITPVMSILKSALAVGSGRVVLVYANRDERSV
IFAAELRDLTARHPDRLTVVHWLESVQGLPRQDQIATLARRFTGGEAYLCGPAPFMAAVQAALAEAGVPRDAVHTEVFRS
LTGDPFAEVAPVPVDGDAADLQVELDGGKHSLRWPRQATLVDAMIAAGLDVPYSCREGQCGSCAATLLRGRVDMPDTEIL
EPEDIEAGTILGCQARPASDEIHIEF
>Mature_346_residues
MIADVDSPSEPRTVRLTVTDVIEESHDARSLVFAVADGARFDYRPGQFLTLRVPGEQGGAVARCYSLASSPHTDAAPKVT
IKRVDGGYGSNWLCDNVVAGQVIEALPPSGVFTPGDLDRDLVLWAGGSGITPVMSILKSALAVGSGRVVLVYANRDERSV
IFAAELRDLTARHPDRLTVVHWLESVQGLPRQDQIATLARRFTGGEAYLCGPAPFMAAVQAALAEAGVPRDAVHTEVFRS
LTGDPFAEVAPVPVDGDAADLQVELDGGKHSLRWPRQATLVDAMIAAGLDVPYSCREGQCGSCAATLLRGRVDMPDTEIL
EPEDIEAGTILGCQARPASDEIHIEF

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=350, Percent_Identity=30.8571428571429, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1787098, Length=346, Percent_Identity=27.1676300578035, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788104, Length=276, Percent_Identity=27.536231884058, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788903, Length=218, Percent_Identity=28.4403669724771, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 36864; Mature: 36864

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIADVDSPSEPRTVRLTVTDVIEESHDARSLVFAVADGARFDYRPGQFLTLRVPGEQGGA
CCCCCCCCCCCCEEEEEEHHHHHCCCCCCEEEEEEECCCEECCCCCCEEEEEECCCCCCH
VARCYSLASSPHTDAAPKVTIKRVDGGYGSNWLCDNVVAGQVIEALPPSGVFTPGDLDRD
HHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
LVLWAGGSGITPVMSILKSALAVGSGRVVLVYANRDERSVIFAAELRDLTARHPDRLTVV
EEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEHHHHHHHCCCCCCHHH
HWLESVQGLPRQDQIATLARRFTGGEAYLCGPAPFMAAVQAALAEAGVPRDAVHTEVFRS
HHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LTGDPFAEVAPVPVDGDAADLQVELDGGKHSLRWPRQATLVDAMIAAGLDVPYSCREGQC
HCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
GSCAATLLRGRVDMPDTEILEPEDIEAGTILGCQARPASDEIHIEF
CHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEC
>Mature Secondary Structure
MIADVDSPSEPRTVRLTVTDVIEESHDARSLVFAVADGARFDYRPGQFLTLRVPGEQGGA
CCCCCCCCCCCCEEEEEEHHHHHCCCCCCEEEEEEECCCEECCCCCCEEEEEECCCCCCH
VARCYSLASSPHTDAAPKVTIKRVDGGYGSNWLCDNVVAGQVIEALPPSGVFTPGDLDRD
HHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
LVLWAGGSGITPVMSILKSALAVGSGRVVLVYANRDERSVIFAAELRDLTARHPDRLTVV
EEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEHHHHHHHCCCCCCHHH
HWLESVQGLPRQDQIATLARRFTGGEAYLCGPAPFMAAVQAALAEAGVPRDAVHTEVFRS
HHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LTGDPFAEVAPVPVDGDAADLQVELDGGKHSLRWPRQATLVDAMIAAGLDVPYSCREGQC
HCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
GSCAATLLRGRVDMPDTEILEPEDIEAGTILGCQARPASDEIHIEF
CHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA