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The map label for this gene is lip2 [H]

Identifier: 108797281

GI number: 108797281

Start: 338204

End: 339154

Strand: Reverse

Name: lip2 [H]

Synonym: Mmcs_0301

Alternate gene names: 108797281

Gene position: 339154-338204 (Counterclockwise)

Preceding gene: 108797282

Following gene: 108797280

Centisome position: 5.94

GC content: 70.98

Gene sequence:

>951_bases
GTGACCGTGCCCGGGCTCGATCCCGACGCCGCCGCGCGTGTCGCCTCGTTCGGCGCCATCCCACCGATGCGCGAGCGTGG
GCTGGACGCGGTGCGGCGCGCCGTGGAGGCGACGCCCCACCCCGATGACACGCCCGAGATGGCGGACGTCGATGACCTGT
CGATTCCCGGCCCGGCGCAACCGATTCCGGTGCGGATCTATCGGCCGACCGACGACGACTCGGCACCGGTGCTGGTCTAC
TTCCACGGCGGCGGCATGGTCATGGGGTCGAACCGCTCGTTCGAACCGCTCGCCCGGGCTCTGGCGGCACACAGCGGTGC
GACGGTGGTCTCCGTCGAGTACCGACTGGCCCCCGAGGCACCGACCCCCGCCCAATTCGACGACGCCTACGCCGCCACCG
TCTGGGTGGCCGGCAATGCCGAGGTGATTCGGGTCGACGCTTCCCGGCTCGCCGTCGTAGGTGACAGCGCCGGCGGATCG
CTGGCAGCGGCGGTCGCGCTGGCGGCCCGCGACCGTGGCGGCCCGTCGATCCTCTGCCAGGTCCTGCTGTACCCGGGCCT
GGACCGAGACATGACCTGCGAGTCGATCCAGCTGCTGGCCGACGCGCCGATGCTGACCCACGCCGACATCGACTACATGC
ACGAACTGGCCGACCAGGGCGCCGCCGTTCCCCCGGATCAGTACCGATTCCCCGCGCTGGCAACAGATCTCACCGGTCTG
CCACCGGCGATCGTTGTGACGGCCGGATGCGACCCGATCCGCGACTGGGGTGAGCGGTACGCAACCCGGTTACGCGACGC
CGGCGTGCAGACCACCCAGACGCGCTATCCCGGCGCGTATCACGGATTCCTGATGCGCTCGGACGCCACCGCCAGGGGTC
GGCTGGCCATCGCGGAGACCGGTGCGCTGCTGCGGGCCAAGTTCGCCTGTCCGGTTCCAGTCTCCCGATGA

Upstream 100 bases:

>100_bases
TGTGCTCCAAGAGCTACGTCAGCGGGCTGTCCCTCGCCGACTGCATCTTCAGCGGTCGGCGCGCGGGCGCGCACGCCGCG
GACGGATTGGGACAGCCACG

Downstream 100 bases:

>100_bases
CCGGAAAGCGGCCGTCGGGGACACCACGGACGGACGCAGAATCTAGACAACCCAGTGCGCCGTGCGCGCCGATGAATGAA
GGAGAGATTCGATGCTCACT

Product: alpha/beta hydrolase fold-3

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTVPGLDPDAAARVASFGAIPPMRERGLDAVRRAVEATPHPDDTPEMADVDDLSIPGPAQPIPVRIYRPTDDDSAPVLVY
FHGGGMVMGSNRSFEPLARALAAHSGATVVSVEYRLAPEAPTPAQFDDAYAATVWVAGNAEVIRVDASRLAVVGDSAGGS
LAAAVALAARDRGGPSILCQVLLYPGLDRDMTCESIQLLADAPMLTHADIDYMHELADQGAAVPPDQYRFPALATDLTGL
PPAIVVTAGCDPIRDWGERYATRLRDAGVQTTQTRYPGAYHGFLMRSDATARGRLAIAETGALLRAKFACPVPVSR

Sequences:

>Translated_316_residues
MTVPGLDPDAAARVASFGAIPPMRERGLDAVRRAVEATPHPDDTPEMADVDDLSIPGPAQPIPVRIYRPTDDDSAPVLVY
FHGGGMVMGSNRSFEPLARALAAHSGATVVSVEYRLAPEAPTPAQFDDAYAATVWVAGNAEVIRVDASRLAVVGDSAGGS
LAAAVALAARDRGGPSILCQVLLYPGLDRDMTCESIQLLADAPMLTHADIDYMHELADQGAAVPPDQYRFPALATDLTGL
PPAIVVTAGCDPIRDWGERYATRLRDAGVQTTQTRYPGAYHGFLMRSDATARGRLAIAETGALLRAKFACPVPVSR
>Mature_315_residues
TVPGLDPDAAARVASFGAIPPMRERGLDAVRRAVEATPHPDDTPEMADVDDLSIPGPAQPIPVRIYRPTDDDSAPVLVYF
HGGGMVMGSNRSFEPLARALAAHSGATVVSVEYRLAPEAPTPAQFDDAYAATVWVAGNAEVIRVDASRLAVVGDSAGGSL
AAAVALAARDRGGPSILCQVLLYPGLDRDMTCESIQLLADAPMLTHADIDYMHELADQGAAVPPDQYRFPALATDLTGLP
PAIVVTAGCDPIRDWGERYATRLRDAGVQTTQTRYPGAYHGFLMRSDATARGRLAIAETGALLRAKFACPVPVSR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=343, Percent_Identity=28.8629737609329, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI157041239, Length=290, Percent_Identity=27.5862068965517, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI61966717, Length=292, Percent_Identity=26.3698630136986, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI21328446, Length=96, Percent_Identity=39.5833333333333, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI68299767, Length=169, Percent_Identity=33.7278106508876, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI68051721, Length=136, Percent_Identity=37.5, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786682, Length=250, Percent_Identity=32, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=138, Percent_Identity=36.9565217391304, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=131, Percent_Identity=35.1145038167939, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71996133, Length=131, Percent_Identity=33.587786259542, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17567059, Length=125, Percent_Identity=34.4, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115533410, Length=94, Percent_Identity=37.2340425531915, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533412, Length=94, Percent_Identity=37.2340425531915, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24656084, Length=100, Percent_Identity=39, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24656076, Length=100, Percent_Identity=39, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130169, Length=100, Percent_Identity=39, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 33367; Mature: 33236

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPGLDPDAAARVASFGAIPPMRERGLDAVRRAVEATPHPDDTPEMADVDDLSIPGPAQ
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PIPVRIYRPTDDDSAPVLVYFHGGGMVMGSNRSFEPLARALAAHSGATVVSVEYRLAPEA
CCCEEEECCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHCCCCEEEEEEEEECCCC
PTPAQFDDAYAATVWVAGNAEVIRVDASRLAVVGDSAGGSLAAAVALAARDRGGPSILCQ
CCCCCCCCCEEEEEEEECCCEEEEEECCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHH
VLLYPGLDRDMTCESIQLLADAPMLTHADIDYMHELADQGAAVPPDQYRFPALATDLTGL
HHHCCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCC
PPAIVVTAGCDPIRDWGERYATRLRDAGVQTTQTRYPGAYHGFLMRSDATARGRLAIAET
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEC
GALLRAKFACPVPVSR
CCEEEEEECCCCCCCC
>Mature Secondary Structure 
TVPGLDPDAAARVASFGAIPPMRERGLDAVRRAVEATPHPDDTPEMADVDDLSIPGPAQ
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PIPVRIYRPTDDDSAPVLVYFHGGGMVMGSNRSFEPLARALAAHSGATVVSVEYRLAPEA
CCCEEEECCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHCCCCEEEEEEEEECCCC
PTPAQFDDAYAATVWVAGNAEVIRVDASRLAVVGDSAGGSLAAAVALAARDRGGPSILCQ
CCCCCCCCCEEEEEEEECCCEEEEEECCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHH
VLLYPGLDRDMTCESIQLLADAPMLTHADIDYMHELADQGAAVPPDQYRFPALATDLTGL
HHHCCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCC
PPAIVVTAGCDPIRDWGERYATRLRDAGVQTTQTRYPGAYHGFLMRSDATARGRLAIAET
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEC
GALLRAKFACPVPVSR
CCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]