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The map label for this gene is kstD [H]

Identifier: 108797267

GI number: 108797267

Start: 324693

End: 326237

Strand: Reverse

Name: kstD [H]

Synonym: Mmcs_0287

Alternate gene names: 108797267

Gene position: 326237-324693 (Counterclockwise)

Preceding gene: 108797272

Following gene: 108797266

Centisome position: 5.72

GC content: 70.36

Gene sequence:

>1545_bases
ATGTCAGCTTTCGACGAGGAGGTCGACGTCGTCGTCGTCGGATCCGGCGGCGGTGTCGCCGGCGCCTACACCGCGGCGCG
CGAAGGACTGTCCGTCGCCCTCGTCGAGGCGACCGAACGCTTCGGCGGAACCACCGCGTACTCCGGTGGCGGCGGCATGT
GGTTCCCGTGCAACCCGGTGGCGCAGCGGGCGGGCATCGACGACAGCCCCGAGGACGCGCTCGCGTACTTCCATCGCGTA
GTCGGTGACCGGACGCCGTTGGACCTGCAGCGGGCCTACGTCCTGGGCGGCCCCCGCCTCATCGAATACCTCGAACGAGA
CGAGCATTTCCAGTTCGCCGAACTCCCCTGGCCGGACTACTTCGGTGCGGTCGAACACGCCCGCGCCGACGGCCGCCGCC
ACATCGTCCCGGTACCGCTGCCCGCCGCCGACGTCGGACCGCAGGCCGCGCAGATCCGCGGACCGCTCGACGCCGAACGC
CTCGGGCATCCAGTCCCCGCACTGTTGACCGGAGGGCGGGCGCTGATCGGGCGCTTCCTCGCCGCGCTCGCTCGGTTCCC
GCACGCACGAACCCATCTGGGTGCGCCGCTGGTCGATCTCGTCGACGTCGACGGCGCGGTCGTCGGGGCGGTGATCAACC
GCGACGGTGAGAACGTCAGGGTTCGCGCACGCCGCGGCGTGCTGCTCGCCGCGGGTGGATTCGAGCAGAACTCCCGGATG
CGCGAATCGTTCGGTGTGCCGGGACGAGCCGAGGACACGATGGGATGTCCCGGCAACACCGGCGCGGCACATCGGGCGGC
GATCCGGGTCGGCGCCGCCACCGACCTCATGGATCAGGCGTGGTGGTCGCCCGGCCTGACCCACCCGGACGGGCGTTCGG
CGTTCGCGCTCTGGTTCACCGGCGGCATCTTCGTCGATCAGCGCGGTGAACGGTTCGTCAACGAATCGGCGCCCTACGAC
CGGCTGGGCCGGGCCGTTATCGCCGAGATGGATTCTCGGGGTATGACTTTGCCGTTCTGGATGGTCTACGACGACCGCGA
GGGCGAGGTGCCTCCGGTCAAGGCCACCAACGTGTCGATGGTCGAGGCCGCACAGTACCGGGACGCCGGTCTGTGGCGTA
CGGCGGACACGCTCGACACACTGGCGGACTTGATCGGGGTCCCGGCGGATGCGCTGACCGCCACGGTCGCCCGGTTCAAC
GCGATGGTCGCCGGCGGGCGCGACACCGACTTCGGCCGCGGTGACGAACCGTATGACCGCGCGTTCTCCGGCGGTAGGTC
GCCGATGGTGGCGATCGAGCAACCGCCTTTCCACGCCGCGGCTTTCGGGGTGTCGGACCTGGGCACCAAGGGCGGACTGC
GTACCGATACCAGCGCACGTGTGCTCGACACCGACGGCAGGCCGATCCCCGGCCTCTACGCTGCGGGCAACACCATGGCC
GCGGTCAGTGGCACGACATATCCGGGTGGCGGCAACCCGATCGGCGCCTCCCTGCTGTTCAGCCATCTCGCCGCGATGGA
CATGGCGGGCGCGGGACCGCGGTGA

Upstream 100 bases:

>100_bases
CATCCAGGGTAGCAACGCGCCGTGACCTGCGCCTTCGTGTGATCCACTGATCGGGACCGACACTTGCCTGCCGTTGCCAT
CACCGGGAAAGGTGCACTCC

Downstream 100 bases:

>100_bases
ACGACGGGCACGAACCGTTGACGGAGTCGGTCCGGTCCCTGATCGACGCCGTCATCCGCACCGAGGTCGACCCCGAGAAG
ATCGCCGCCGCACACGCCCA

Product: 3-ketosteroid-delta-1-dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSAFDEEVDVVVVGSGGGVAGAYTAAREGLSVALVEATERFGGTTAYSGGGGMWFPCNPVAQRAGIDDSPEDALAYFHRV
VGDRTPLDLQRAYVLGGPRLIEYLERDEHFQFAELPWPDYFGAVEHARADGRRHIVPVPLPAADVGPQAAQIRGPLDAER
LGHPVPALLTGGRALIGRFLAALARFPHARTHLGAPLVDLVDVDGAVVGAVINRDGENVRVRARRGVLLAAGGFEQNSRM
RESFGVPGRAEDTMGCPGNTGAAHRAAIRVGAATDLMDQAWWSPGLTHPDGRSAFALWFTGGIFVDQRGERFVNESAPYD
RLGRAVIAEMDSRGMTLPFWMVYDDREGEVPPVKATNVSMVEAAQYRDAGLWRTADTLDTLADLIGVPADALTATVARFN
AMVAGGRDTDFGRGDEPYDRAFSGGRSPMVAIEQPPFHAAAFGVSDLGTKGGLRTDTSARVLDTDGRPIPGLYAAGNTMA
AVSGTTYPGGGNPIGASLLFSHLAAMDMAGAGPR

Sequences:

>Translated_514_residues
MSAFDEEVDVVVVGSGGGVAGAYTAAREGLSVALVEATERFGGTTAYSGGGGMWFPCNPVAQRAGIDDSPEDALAYFHRV
VGDRTPLDLQRAYVLGGPRLIEYLERDEHFQFAELPWPDYFGAVEHARADGRRHIVPVPLPAADVGPQAAQIRGPLDAER
LGHPVPALLTGGRALIGRFLAALARFPHARTHLGAPLVDLVDVDGAVVGAVINRDGENVRVRARRGVLLAAGGFEQNSRM
RESFGVPGRAEDTMGCPGNTGAAHRAAIRVGAATDLMDQAWWSPGLTHPDGRSAFALWFTGGIFVDQRGERFVNESAPYD
RLGRAVIAEMDSRGMTLPFWMVYDDREGEVPPVKATNVSMVEAAQYRDAGLWRTADTLDTLADLIGVPADALTATVARFN
AMVAGGRDTDFGRGDEPYDRAFSGGRSPMVAIEQPPFHAAAFGVSDLGTKGGLRTDTSARVLDTDGRPIPGLYAAGNTMA
AVSGTTYPGGGNPIGASLLFSHLAAMDMAGAGPR
>Mature_513_residues
SAFDEEVDVVVVGSGGGVAGAYTAAREGLSVALVEATERFGGTTAYSGGGGMWFPCNPVAQRAGIDDSPEDALAYFHRVV
GDRTPLDLQRAYVLGGPRLIEYLERDEHFQFAELPWPDYFGAVEHARADGRRHIVPVPLPAADVGPQAAQIRGPLDAERL
GHPVPALLTGGRALIGRFLAALARFPHARTHLGAPLVDLVDVDGAVVGAVINRDGENVRVRARRGVLLAAGGFEQNSRMR
ESFGVPGRAEDTMGCPGNTGAAHRAAIRVGAATDLMDQAWWSPGLTHPDGRSAFALWFTGGIFVDQRGERFVNESAPYDR
LGRAVIAEMDSRGMTLPFWMVYDDREGEVPPVKATNVSMVEAAQYRDAGLWRTADTLDTLADLIGVPADALTATVARFNA
MVAGGRDTDFGRGDEPYDRAFSGGRSPMVAIEQPPFHAAAFGVSDLGTKGGLRTDTSARVLDTDGRPIPGLYAAGNTMAA
VSGTTYPGGGNPIGASLLFSHLAAMDMAGAGPR

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 54280; Mature: 54148

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFDEEVDVVVVGSGGGVAGAYTAAREGLSVALVEATERFGGTTAYSGGGGMWFPCNPV
CCCCCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEEHHHHHCCCEEECCCCCCCCCCCHH
AQRAGIDDSPEDALAYFHRVVGDRTPLDLQRAYVLGGPRLIEYLERDEHFQFAELPWPDY
HHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHEECCCHHHHHHHHHCCCCEEECCCCCHH
FGAVEHARADGRRHIVPVPLPAADVGPQAAQIRGPLDAERLGHPVPALLTGGRALIGRFL
HHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHCCCCCHHHHCCCCHHHHHCCHHHHHHHH
AALARFPHARTHLGAPLVDLVDVDGAVVGAVINRDGENVRVRARRGVLLAAGGFEQNSRM
HHHHHCCCHHHHCCCCHHEEEECCCCEEEEEECCCCCCEEEEEECCEEEEECCCCCCHHH
RESFGVPGRAEDTMGCPGNTGAAHRAAIRVGAATDLMDQAWWSPGLTHPDGRSAFALWFT
HHHCCCCCCCCCCCCCCCCCCCHHHHEEEECCHHHHHHHHHCCCCCCCCCCCCEEEEEEE
GGIFVDQRGERFVNESAPYDRLGRAVIAEMDSRGMTLPFWMVYDDREGEVPPVKATNVSM
CCEEECCCCCHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCHH
VEAAQYRDAGLWRTADTLDTLADLIGVPADALTATVARFNAMVAGGRDTDFGRGDEPYDR
HHHHHHHCCCCEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHH
AFSGGRSPMVAIEQPPFHAAAFGVSDLGTKGGLRTDTSARVLDTDGRPIPGLYAAGNTMA
HHCCCCCCEEEEECCCCHHHHHCHHHCCCCCCCCCCCCCEEEECCCCCCCCEEECCCEEE
AVSGTTYPGGGNPIGASLLFSHLAAMDMAGAGPR
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SAFDEEVDVVVVGSGGGVAGAYTAAREGLSVALVEATERFGGTTAYSGGGGMWFPCNPV
CCCCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEEHHHHHCCCEEECCCCCCCCCCCHH
AQRAGIDDSPEDALAYFHRVVGDRTPLDLQRAYVLGGPRLIEYLERDEHFQFAELPWPDY
HHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHEECCCHHHHHHHHHCCCCEEECCCCCHH
FGAVEHARADGRRHIVPVPLPAADVGPQAAQIRGPLDAERLGHPVPALLTGGRALIGRFL
HHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHCCCCCHHHHCCCCHHHHHCCHHHHHHHH
AALARFPHARTHLGAPLVDLVDVDGAVVGAVINRDGENVRVRARRGVLLAAGGFEQNSRM
HHHHHCCCHHHHCCCCHHEEEECCCCEEEEEECCCCCCEEEEEECCEEEEECCCCCCHHH
RESFGVPGRAEDTMGCPGNTGAAHRAAIRVGAATDLMDQAWWSPGLTHPDGRSAFALWFT
HHHCCCCCCCCCCCCCCCCCCCHHHHEEEECCHHHHHHHHHCCCCCCCCCCCCEEEEEEE
GGIFVDQRGERFVNESAPYDRLGRAVIAEMDSRGMTLPFWMVYDDREGEVPPVKATNVSM
CCEEECCCCCHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCHH
VEAAQYRDAGLWRTADTLDTLADLIGVPADALTATVARFNAMVAGGRDTDFGRGDEPYDR
HHHHHHHCCCCEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHH
AFSGGRSPMVAIEQPPFHAAAFGVSDLGTKGGLRTDTSARVLDTDGRPIPGLYAAGNTMA
HHCCCCCCEEEEECCCCHHHHHCHHHCCCCCCCCCCCCCEEEECCCCCCCCEEECCCEEE
AVSGTTYPGGGNPIGASLLFSHLAAMDMAGAGPR
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]