Click here to switch to the map view.

The map label for this gene is sdhA [C]

Identifier: 108797233

GI number: 108797233

Start: 292950

End: 294896

Strand: Reverse

Name: sdhA [C]

Synonym: Mmcs_0253

Alternate gene names: 108797233

Gene position: 294896-292950 (Counterclockwise)

Preceding gene: 108797234

Following gene: 108797232

Centisome position: 5.17

GC content: 67.08

Gene sequence:

>1947_bases
ATGGCGGCGAGCGACCCCCGACCCGGCGACTCCAGCGGTATCGAGAGACACCAGTACGACGTGGTGGTCATCGGTGCCGG
CGGCGCGGGTCTGCGAGCAGTGATCGAAGCACGCGAACGCGGCCTGAAGGTCGCCGTGGTGTGCAAATCGCTGTTCGGCA
AGGCGCACACGGTGATGGCCGAGGGCGGCTGCGCCGCATCGATGGGCAATACGAACCCGAAGGACAACTGGCAGGTCCAC
TTCGGCGACACCATGCGTGGCGGCAAATTCCTCAACAACTGGCGGATGGCCGAGTTGCACGCCAAGGAGGCGCCCGACCG
GGTGTGGGAACTGGAGACCTACGGCGCGCTGTTCGACCGCACCAAGGACGGCCGGATCAGCCAGCGCAACTTCGGCGGTC
ACACCTATCCGCGGCTGGCCCACGTCGGTGACCGCACCGGCCTGGAGATCATCCGCACCATGCAGCAGAAGATCGTCTCG
CTGCAGCAGGAGGACAAGGAGGAACTCGGCGACTACGAGGCCCGCATCCGGGTGTTCGCCGAGTGCACCGTCACCGATCT
GATCAAAGACGGGGATCGGATCGCGGGAGCCTTCGGCTACTGGCGTGAGAGCGGCAGCTTCATCCTCTTCGACGCACCCG
CGGTCGTGCTCGCCACGGGCGGAATCGGCAAGTCCTACAAGGTCACCTCGAACTCGTGGGAGTACACCGGCGACGGACAC
GCCCTGGCGCTGCGGGCCGGCGCGACGCTGATCAACATGGAGTTCATCCAGTTCCACCCCACCGGCATGGTGTGGCCGCC
CAGCGTGAAGGGCATCCTCGTCACCGAGGGTGTGCGCGGTGACGGCGGTGTGCTGAAGAACTCCGACGGCAAGCGGTTCA
TGTTCGACTACATCCCCGACGTGTTCAAGGGCCAGTACGCCGAGTCCGAGGACGAGGCCGACCAGTGGCTCAAGGACAAC
GACTCCGCGCGCCGCACCCCCGACCTGCTGCCCCGCGACGAGGTGGCCCGCGCGATCAACACCGAGGTGAAGGAAGGTCG
CGGCACCCCGCACGGCGGCGTCTACCTCGACATCGCGTCGCGCATGAGCTCCGAGGAGATCAAGCGGCGGCTGCCGTCGA
TGTACCACCAGTTCATGGAGCTCGCCGAGGTCGACATCACCACCGACGAGATGGAGGTCGGGCCCACCTGCCACTACGTG
ATGGGTGGCATCGAGGTCGACCCGGACACCGGCGCCGCCAAGACGCCGGGCCTGTTCGCCGCCGGCGAGTGCGCCGGCGG
TATGCACGGGTCGAACCGGTTGGGCGGCAACTCGCTGTCGGACCTGCTGGTCTTCGGCCGCCGTGCCGGACTGGGCGCCT
CGGACTACGTACGTGCCCTCGGCAACCGGCCGACGGTGTCGGAGGAGGCCGTCGAGGCGGCGCTCAAACTGGCGCTCGCC
CCCTTTGACGGGCCGAGCGAGGGTCAACCGGAGAACCCCTACACCATGCAGCTCGACCTGCAGGACACGATGAACGAGCT
GGTCGGGATCATCCGCAAGGCCGACGAGATCATCGAGGCCCAGGGAAAACTCGACGAACTGCGCGATCGGTTCAAGCGCA
TGAAGGTCGAGGGCAACCGGCACTTCAACCCCGGCTGGCACCTCGCGATCGACCTGCGCAACATGCTGCTGGTCAGCGAG
TGCGTGGCCAAGGCGGCGCTGGAACGCACCGAGAGCCGCGGCGGCCACACCCGCGACGACTACCCGTCGATGGAAGCGGA
GTGGCGCAGAACGCTGCTGGTGTGCCGGGCCCAGGGGGACGACGTCGTCGTCCCGAGCGTCACCGTCACCCGCGAGGACC
AGGTGCCGATGCGCGACGACCTGCTCGAGCTGCTGGACATCGAAGAACTCCAGAAGTACTACACCGACGAGGAACTCGCC
AACCACCCGGCAAGGAGGGGCTCATGA

Upstream 100 bases:

>100_bases
ACTTCTACGTCATGCTGGTCGCCAGCGGCACCATCTCCGACCTGAGATTCATTGGCTGAGTAGTGATTTCACACACGCAC
GAAACGACGAGTGAGGTTTG

Downstream 100 bases:

>100_bases
GCTACCAGGCGAAGATGCGGGTGTGGCGCGGCGACGACGAGGGCGGCGCACTGCAGGACTACACCGTCGAGGTCAACGAG
GGTGAGGTCGTCCTCGACAT

Product: succinate dehydrogenase flavoprotein subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MAASDPRPGDSSGIERHQYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGKAHTVMAEGGCAASMGNTNPKDNWQVH
FGDTMRGGKFLNNWRMAELHAKEAPDRVWELETYGALFDRTKDGRISQRNFGGHTYPRLAHVGDRTGLEIIRTMQQKIVS
LQQEDKEELGDYEARIRVFAECTVTDLIKDGDRIAGAFGYWRESGSFILFDAPAVVLATGGIGKSYKVTSNSWEYTGDGH
ALALRAGATLINMEFIQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSDGKRFMFDYIPDVFKGQYAESEDEADQWLKDN
DSARRTPDLLPRDEVARAINTEVKEGRGTPHGGVYLDIASRMSSEEIKRRLPSMYHQFMELAEVDITTDEMEVGPTCHYV
MGGIEVDPDTGAAKTPGLFAAGECAGGMHGSNRLGGNSLSDLLVFGRRAGLGASDYVRALGNRPTVSEEAVEAALKLALA
PFDGPSEGQPENPYTMQLDLQDTMNELVGIIRKADEIIEAQGKLDELRDRFKRMKVEGNRHFNPGWHLAIDLRNMLLVSE
CVAKAALERTESRGGHTRDDYPSMEAEWRRTLLVCRAQGDDVVVPSVTVTREDQVPMRDDLLELLDIEELQKYYTDEELA
NHPARRGS

Sequences:

>Translated_648_residues
MAASDPRPGDSSGIERHQYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGKAHTVMAEGGCAASMGNTNPKDNWQVH
FGDTMRGGKFLNNWRMAELHAKEAPDRVWELETYGALFDRTKDGRISQRNFGGHTYPRLAHVGDRTGLEIIRTMQQKIVS
LQQEDKEELGDYEARIRVFAECTVTDLIKDGDRIAGAFGYWRESGSFILFDAPAVVLATGGIGKSYKVTSNSWEYTGDGH
ALALRAGATLINMEFIQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSDGKRFMFDYIPDVFKGQYAESEDEADQWLKDN
DSARRTPDLLPRDEVARAINTEVKEGRGTPHGGVYLDIASRMSSEEIKRRLPSMYHQFMELAEVDITTDEMEVGPTCHYV
MGGIEVDPDTGAAKTPGLFAAGECAGGMHGSNRLGGNSLSDLLVFGRRAGLGASDYVRALGNRPTVSEEAVEAALKLALA
PFDGPSEGQPENPYTMQLDLQDTMNELVGIIRKADEIIEAQGKLDELRDRFKRMKVEGNRHFNPGWHLAIDLRNMLLVSE
CVAKAALERTESRGGHTRDDYPSMEAEWRRTLLVCRAQGDDVVVPSVTVTREDQVPMRDDLLELLDIEELQKYYTDEELA
NHPARRGS
>Mature_647_residues
AASDPRPGDSSGIERHQYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGKAHTVMAEGGCAASMGNTNPKDNWQVHF
GDTMRGGKFLNNWRMAELHAKEAPDRVWELETYGALFDRTKDGRISQRNFGGHTYPRLAHVGDRTGLEIIRTMQQKIVSL
QQEDKEELGDYEARIRVFAECTVTDLIKDGDRIAGAFGYWRESGSFILFDAPAVVLATGGIGKSYKVTSNSWEYTGDGHA
LALRAGATLINMEFIQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSDGKRFMFDYIPDVFKGQYAESEDEADQWLKDND
SARRTPDLLPRDEVARAINTEVKEGRGTPHGGVYLDIASRMSSEEIKRRLPSMYHQFMELAEVDITTDEMEVGPTCHYVM
GGIEVDPDTGAAKTPGLFAAGECAGGMHGSNRLGGNSLSDLLVFGRRAGLGASDYVRALGNRPTVSEEAVEAALKLALAP
FDGPSEGQPENPYTMQLDLQDTMNELVGIIRKADEIIEAQGKLDELRDRFKRMKVEGNRHFNPGWHLAIDLRNMLLVSEC
VAKAALERTESRGGHTRDDYPSMEAEWRRTLLVCRAQGDDVVVPSVTVTREDQVPMRDDLLELLDIEELQKYYTDEELAN
HPARRGS

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=572, Percent_Identity=33.5664335664336, Blast_Score=265, Evalue=1e-70,
Organism=Escherichia coli, GI1786942, Length=595, Percent_Identity=35.9663865546218, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1790597, Length=543, Percent_Identity=34.8066298342541, Blast_Score=271, Evalue=9e-74,
Organism=Escherichia coli, GI1788928, Length=586, Percent_Identity=31.3993174061433, Blast_Score=221, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17505833, Length=568, Percent_Identity=34.5070422535211, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17550100, Length=566, Percent_Identity=34.452296819788, Blast_Score=279, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI71986328, Length=449, Percent_Identity=25.8351893095768, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322416, Length=614, Percent_Identity=34.3648208469055, Blast_Score=287, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6322701, Length=613, Percent_Identity=34.42088091354, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6322511, Length=483, Percent_Identity=24.6376811594203, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320788, Length=479, Percent_Identity=27.348643006263, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17137288, Length=626, Percent_Identity=32.5878594249201, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24655642, Length=626, Percent_Identity=32.5878594249201, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24655647, Length=626, Percent_Identity=32.5878594249201, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24663005, Length=605, Percent_Identity=33.0578512396694, Blast_Score=260, Evalue=3e-69,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 71527; Mature: 71396

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASDPRPGDSSGIERHQYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGKAHTVMA
CCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHCCCEEEEE
EGGCAASMGNTNPKDNWQVHFGDTMRGGKFLNNWRMAELHAKEAPDRVWELETYGALFDR
CCCCEECCCCCCCCCCEEEEECCCCCCCCHHCCCEEEEHHHHCCCCHHEEHHHHHHHHHC
TKDGRISQRNFGGHTYPRLAHVGDRTGLEIIRTMQQKIVSLQQEDKEELGDYEARIRVFA
CCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEE
ECTVTDLIKDGDRIAGAFGYWRESGSFILFDAPAVVLATGGIGKSYKVTSNSWEYTGDGH
HHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCEEEECCCCEECCCCC
ALALRAGATLINMEFIQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSDGKRFMFDYIPD
EEEEECCCEEEEEEEEEECCCCEECCCCCCEEEEECCCCCCCCEEECCCCCEEEHHHCCH
VFKGQYAESEDEADQWLKDNDSARRTPDLLPRDEVARAINTEVKEGRGTPHGGVYLDIAS
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHH
RMSSEEIKRRLPSMYHQFMELAEVDITTDEMEVGPTCHYVMGGIEVDPDTGAAKTPGLFA
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEECCCCCCCCCCCCEE
AGECAGGMHGSNRLGGNSLSDLLVFGRRAGLGASDYVRALGNRPTVSEEAVEAALKLALA
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCC
PFDGPSEGQPENPYTMQLDLQDTMNELVGIIRKADEIIEAQGKLDELRDRFKRMKVEGNR
CCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
HFNPGWHLAIDLRNMLLVSECVAKAALERTESRGGHTRDDYPSMEAEWRRTLLVCRAQGD
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEEEEEEECCC
DVVVPSVTVTREDQVPMRDDLLELLDIEELQKYYTDEELANHPARRGS
CEEECCEEEECCCCCCHHHHHHHHHCHHHHHHHHCHHHHHCCCCCCCC
>Mature Secondary Structure 
AASDPRPGDSSGIERHQYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGKAHTVMA
CCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHCCCEEEEE
EGGCAASMGNTNPKDNWQVHFGDTMRGGKFLNNWRMAELHAKEAPDRVWELETYGALFDR
CCCCEECCCCCCCCCCEEEEECCCCCCCCHHCCCEEEEHHHHCCCCHHEEHHHHHHHHHC
TKDGRISQRNFGGHTYPRLAHVGDRTGLEIIRTMQQKIVSLQQEDKEELGDYEARIRVFA
CCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEE
ECTVTDLIKDGDRIAGAFGYWRESGSFILFDAPAVVLATGGIGKSYKVTSNSWEYTGDGH
HHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCEEEECCCCEECCCCC
ALALRAGATLINMEFIQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSDGKRFMFDYIPD
EEEEECCCEEEEEEEEEECCCCEECCCCCCEEEEECCCCCCCCEEECCCCCEEEHHHCCH
VFKGQYAESEDEADQWLKDNDSARRTPDLLPRDEVARAINTEVKEGRGTPHGGVYLDIAS
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHH
RMSSEEIKRRLPSMYHQFMELAEVDITTDEMEVGPTCHYVMGGIEVDPDTGAAKTPGLFA
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEECCCCCCCCCCCCEE
AGECAGGMHGSNRLGGNSLSDLLVFGRRAGLGASDYVRALGNRPTVSEEAVEAALKLALA
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCC
PFDGPSEGQPENPYTMQLDLQDTMNELVGIIRKADEIIEAQGKLDELRDRFKRMKVEGNR
CCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
HFNPGWHLAIDLRNMLLVSECVAKAALERTESRGGHTRDDYPSMEAEWRRTLLVCRAQGD
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEEEEEEECCC
DVVVPSVTVTREDQVPMRDDLLELLDIEELQKYYTDEELANHPARRGS
CEEECCEEEECCCCCCHHHHHHHHHCHHHHHHHHCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]