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The map label for this gene is fadD21 [H]

Identifier: 108797227

GI number: 108797227

Start: 287707

End: 289479

Strand: Direct

Name: fadD21 [H]

Synonym: Mmcs_0247

Alternate gene names: 108797227

Gene position: 287707-289479 (Clockwise)

Preceding gene: 108797226

Following gene: 108797228

Centisome position: 5.04

GC content: 68.58

Gene sequence:

>1773_bases
ATGACACCGACGTCCGAGTCGTCGATGCTGTCGATGCTGCACGGGCGGGCCAGCCTGCGCCCCGACGACATCGCCTTCAC
CTTCACCCACTACGACCGCGACCCGGCGGGCGCCCCCGAGACGCTGACGTGGGCACAGCTGGCCCGCCGGACGATGAGCG
TGGCACGCGAGATCCGCCAGTACGGGTCATTCGGTGACCGGGCGGTGATCCTCGCGCCCCAGGGGTTGGAATACGTCCTG
GCGTTCCTGGGCGCCATGCAGGCCGGGCTCGTGGCGGTCCCGCTCCCGCTGCCGCACCGGGGCTCGGGTCATGACCGGGT
GAGCGCGGTGCTCACCGACACGAAACCGGCCGTCGTCTTCACGACGTCGTCGACCGCGGGCGACGTCGGCGATTTCGTCG
ATGCGTCACCGTTGGACACCGTGCCGAAGATCGTCGAGATCGATGCGCTGAACCTGGACGCCGAGGGCGAGATCCGCCTC
GATCCGGCCGGCCTTCCCGCCACGGCGTATCTGCAGTACAGCTCCGGTTCCACCCGGCTGCCGACCGGGGCCATGATCTC
GCACCGCAACCTGCAGGCGAACTACGAGCAGGTGATGCGCACCTTCTTCGCCGGCGATGGACCCTCGAGCACCACCACGT
TCGTATCGTGGTTGCCCTTCTATCACGACATGGGTCTGGTGCTGGGCATCTGCGTACCGATCCTCAGCGGCCATCCCGCC
GCGTTGACCAGTCCGGTCGCCTTCCTGGAAAGGCCGGCCAGGTGGATACGAGCGCTGGCCGAGAACCCGCACGCCTTCTC
GGCGGCGCCCAACTTCGCCTTCGACCTGGCCGCCCGCAAGACCAGCGACAGTGACCTCGCCGGTCTCGACCTCGGCGGGG
TGCGGGGAATCATCAACGGCGCCGAGCGGGTCGAGCCGGCCACCCTGGAACGCTTCGCGGATCGGTTCGCCCACTTCGAC
TTCCACGACCACATGCTGCGTCCGTCATACGGCCTGGCCGAGGCGACGGTCTTCGTGGCATCGGGCACGTGGAGCGAGAC
GTCACCGGCCGCACACTTCGATGCCGACGAGCTGGGCGTCGGCCGCGTGGTGCCGTGCGCACCCGGGAAGGGCACGGCGC
TGGTCAAATACGACGTGCCGCAGTCACCGGTCGTGCGGATCGTCGACACCGACACCCACCGGCAGTGCCCCGATGACGTG
GTGGGGGAGATCTGGGTGCACGGAGACAACGTCGCGGCCGGCTACTGGAGCAGGCCGCCGGAGGATCAGCGGTGCTTCGG
CGCCACACTCGTCGACCCGTCGCCCGGCACACCGGGCGGGACGTGGCTTCGAACCGGCGACCTCGGGTTCATCCACGAGG
GCGAGCTGTTCATCGTCGGCCGCATCAAGGACCTTCTCATCATCCGCGGCCGCAACCACCATCCCGAGGACATCGAGGCG
ACGGTGCAGGAGATCACCCGCGGTCGGGTCGCGGCGGTATCGGTCCCGGTGAACAGCACCGAAAGCCTCGTCACCGTCAT
CGAACTCAAGGAGCCGCCCGTCCTCGACGAGGATGCGTTGCGCCGCTTCGGTGAGCTCAAGAGTGACGTCACCGCCGCGA
TCTCCAACGCGCACGGCATCAGCGTCGGTGACGTCGTGCTGGTGGCATCCGGGTCGATTCCCACCACGACGAGCGGCAAG
ATCCGGCGCACCGCCTCCGCGGAGTTGTATCGGAAGGACGGGTTCGCCCGGCTCGACGGCGGGAAGGGGCAGCATGGGCA
CCTGGCGGGGTAA

Upstream 100 bases:

>100_bases
TCACGGTGCCCGCCATCGCCGCACTCGTCGGGCGCGCGAACTGGTGGCCGTCGCGAGTCACCGCATCGCCCGGCGCCGAG
CGGATCGGTGCGCACCCGTG

Downstream 100 bases:

>100_bases
TGTCGGGCCCGGTGCGGCCGAGCAGGGCGGGAAGGTGCGCGAAACGATTATGAGACGGCTTCTCGCATTCGGTTCTGCGC
TGACGATCATCGGCGCCGCA

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MTPTSESSMLSMLHGRASLRPDDIAFTFTHYDRDPAGAPETLTWAQLARRTMSVAREIRQYGSFGDRAVILAPQGLEYVL
AFLGAMQAGLVAVPLPLPHRGSGHDRVSAVLTDTKPAVVFTTSSTAGDVGDFVDASPLDTVPKIVEIDALNLDAEGEIRL
DPAGLPATAYLQYSSGSTRLPTGAMISHRNLQANYEQVMRTFFAGDGPSSTTTFVSWLPFYHDMGLVLGICVPILSGHPA
ALTSPVAFLERPARWIRALAENPHAFSAAPNFAFDLAARKTSDSDLAGLDLGGVRGIINGAERVEPATLERFADRFAHFD
FHDHMLRPSYGLAEATVFVASGTWSETSPAAHFDADELGVGRVVPCAPGKGTALVKYDVPQSPVVRIVDTDTHRQCPDDV
VGEIWVHGDNVAAGYWSRPPEDQRCFGATLVDPSPGTPGGTWLRTGDLGFIHEGELFIVGRIKDLLIIRGRNHHPEDIEA
TVQEITRGRVAAVSVPVNSTESLVTVIELKEPPVLDEDALRRFGELKSDVTAAISNAHGISVGDVVLVASGSIPTTTSGK
IRRTASAELYRKDGFARLDGGKGQHGHLAG

Sequences:

>Translated_590_residues
MTPTSESSMLSMLHGRASLRPDDIAFTFTHYDRDPAGAPETLTWAQLARRTMSVAREIRQYGSFGDRAVILAPQGLEYVL
AFLGAMQAGLVAVPLPLPHRGSGHDRVSAVLTDTKPAVVFTTSSTAGDVGDFVDASPLDTVPKIVEIDALNLDAEGEIRL
DPAGLPATAYLQYSSGSTRLPTGAMISHRNLQANYEQVMRTFFAGDGPSSTTTFVSWLPFYHDMGLVLGICVPILSGHPA
ALTSPVAFLERPARWIRALAENPHAFSAAPNFAFDLAARKTSDSDLAGLDLGGVRGIINGAERVEPATLERFADRFAHFD
FHDHMLRPSYGLAEATVFVASGTWSETSPAAHFDADELGVGRVVPCAPGKGTALVKYDVPQSPVVRIVDTDTHRQCPDDV
VGEIWVHGDNVAAGYWSRPPEDQRCFGATLVDPSPGTPGGTWLRTGDLGFIHEGELFIVGRIKDLLIIRGRNHHPEDIEA
TVQEITRGRVAAVSVPVNSTESLVTVIELKEPPVLDEDALRRFGELKSDVTAAISNAHGISVGDVVLVASGSIPTTTSGK
IRRTASAELYRKDGFARLDGGKGQHGHLAG
>Mature_589_residues
TPTSESSMLSMLHGRASLRPDDIAFTFTHYDRDPAGAPETLTWAQLARRTMSVAREIRQYGSFGDRAVILAPQGLEYVLA
FLGAMQAGLVAVPLPLPHRGSGHDRVSAVLTDTKPAVVFTTSSTAGDVGDFVDASPLDTVPKIVEIDALNLDAEGEIRLD
PAGLPATAYLQYSSGSTRLPTGAMISHRNLQANYEQVMRTFFAGDGPSSTTTFVSWLPFYHDMGLVLGICVPILSGHPAA
LTSPVAFLERPARWIRALAENPHAFSAAPNFAFDLAARKTSDSDLAGLDLGGVRGIINGAERVEPATLERFADRFAHFDF
HDHMLRPSYGLAEATVFVASGTWSETSPAAHFDADELGVGRVVPCAPGKGTALVKYDVPQSPVVRIVDTDTHRQCPDDVV
GEIWVHGDNVAAGYWSRPPEDQRCFGATLVDPSPGTPGGTWLRTGDLGFIHEGELFIVGRIKDLLIIRGRNHHPEDIEAT
VQEITRGRVAAVSVPVNSTESLVTVIELKEPPVLDEDALRRFGELKSDVTAAISNAHGISVGDVVLVASGSIPTTTSGKI
RRTASAELYRKDGFARLDGGKGQHGHLAG

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=594, Percent_Identity=25.7575757575758, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI225735629, Length=593, Percent_Identity=25.2951096121417, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI45827692, Length=593, Percent_Identity=25.2951096121417, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI55749758, Length=537, Percent_Identity=24.3947858472998, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI1788107, Length=559, Percent_Identity=26.1180679785331, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193202679, Length=608, Percent_Identity=22.6973684210526, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=557, Percent_Identity=23.6983842010772, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24654656, Length=517, Percent_Identity=23.0174081237911, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI18859661, Length=487, Percent_Identity=25.4620123203285, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63190; Mature: 63059

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTSESSMLSMLHGRASLRPDDIAFTFTHYDRDPAGAPETLTWAQLARRTMSVAREIRQ
CCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YGSFGDRAVILAPQGLEYVLAFLGAMQAGLVAVPLPLPHRGSGHDRVSAVLTDTKPAVVF
HCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEEEECCCCEEEE
TTSSTAGDVGDFVDASPLDTVPKIVEIDALNLDAEGEIRLDPAGLPATAYLQYSSGSTRL
EECCCCCCCHHCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCEEEEEEECCCCCCC
PTGAMISHRNLQANYEQVMRTFFAGDGPSSTTTFVSWLPFYHDMGLVLGICVPILSGHPA
CCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ALTSPVAFLERPARWIRALAENPHAFSAAPNFAFDLAARKTSDSDLAGLDLGGVRGIING
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHCCCCCCCCCCCCCCCHHHHHCC
AERVEPATLERFADRFAHFDFHDHMLRPSYGLAEATVFVASGTWSETSPAAHFDADELGV
HHHCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHCCC
GRVVPCAPGKGTALVKYDVPQSPVVRIVDTDTHRQCPDDVVGEIWVHGDNVAAGYWSRPP
CCEEECCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHCEEEEECCCEEECCCCCCC
EDQRCFGATLVDPSPGTPGGTWLRTGDLGFIHEGELFIVGRIKDLLIIRGRNHHPEDIEA
CCCHHCCCEEECCCCCCCCCCEEEECCCCEEECCCEEEEEEECEEEEEECCCCCCHHHHH
TVQEITRGRVAAVSVPVNSTESLVTVIELKEPPVLDEDALRRFGELKSDVTAAISNAHGI
HHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
SVGDVVLVASGSIPTTTSGKIRRTASAELYRKDGFARLDGGKGQHGHLAG
EECCEEEEECCCCCCCCCCCEEECHHHHHHHHCCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
TPTSESSMLSMLHGRASLRPDDIAFTFTHYDRDPAGAPETLTWAQLARRTMSVAREIRQ
CCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YGSFGDRAVILAPQGLEYVLAFLGAMQAGLVAVPLPLPHRGSGHDRVSAVLTDTKPAVVF
HCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEEEECCCCEEEE
TTSSTAGDVGDFVDASPLDTVPKIVEIDALNLDAEGEIRLDPAGLPATAYLQYSSGSTRL
EECCCCCCCHHCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCEEEEEEECCCCCCC
PTGAMISHRNLQANYEQVMRTFFAGDGPSSTTTFVSWLPFYHDMGLVLGICVPILSGHPA
CCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ALTSPVAFLERPARWIRALAENPHAFSAAPNFAFDLAARKTSDSDLAGLDLGGVRGIING
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHCCCCCCCCCCCCCCCHHHHHCC
AERVEPATLERFADRFAHFDFHDHMLRPSYGLAEATVFVASGTWSETSPAAHFDADELGV
HHHCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHCCC
GRVVPCAPGKGTALVKYDVPQSPVVRIVDTDTHRQCPDDVVGEIWVHGDNVAAGYWSRPP
CCEEECCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHCEEEEECCCEEECCCCCCC
EDQRCFGATLVDPSPGTPGGTWLRTGDLGFIHEGELFIVGRIKDLLIIRGRNHHPEDIEA
CCCHHCCCEEECCCCCCCCCCEEEECCCCEEECCCEEEEEEECEEEEEECCCCCCHHHHH
TVQEITRGRVAAVSVPVNSTESLVTVIELKEPPVLDEDALRRFGELKSDVTAAISNAHGI
HHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
SVGDVVLVASGSIPTTTSGKIRRTASAELYRKDGFARLDGGKGQHGHLAG
EECCEEEEECCCCCCCCCCCEEECHHHHHHHHCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]