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The map label for this gene is trmD

Identifier: 108763843

GI number: 108763843

Start: 4131868

End: 4132611

Strand: Direct

Name: trmD

Synonym: MXAN_3548

Alternate gene names: 108763843

Gene position: 4131868-4132611 (Clockwise)

Preceding gene: 108760176

Following gene: 108762775

Centisome position: 45.21

GC content: 68.95

Gene sequence:

>744_bases
ATGAGCCCACCGTATCCGGTGGAGATCCTCACGTTGTTCCCCGGCATGGTGACCGGTTACCTGGGGGCCAGCATCCTCGG
GAAGGCTCAGGAGAAGGGCCTGCTGGCCACCACCATCACCGATATCCGCGAGTACGCCGAGGGCAAGCACCGCGTTACCG
ACGACGCACCCTACGGCGGTGGCGCCGGCATGGTGATGAAGCCGGAGCCCCTGGTCGCCGCGATTGAGGCGGCCAAAGCG
CGGCATCCGGGCGCGCGGGTGCTGCTCATGAGCCCCCGAGGGCCCACCTTCACCCAGGCCACTGCGCGTGAGCTGGTCCG
TCACGAGGCGGGCCTCATCCTGGTCTGCGGCCGCTACGAGGGCGTGGACGAGCGGGTGATGGCGCACCTCGACGGGGAGC
TGTCTCTGGGGGACTTCGTTCTCACTGGCGGCGAGCTGGCGGCCATGACGGTGGTGGATGCGGTGGCGCGGCTGGTGCCC
GGGGTGCTGGGCAACGTGGACTCGTCCGTCACGGAGAGCTTCGAGGAAGGCCTGCTGGAGCATCCCCAGTACACCCGCCC
GCCTGTCTTCCGGGGCGTCGAGGTGCCGGCCGCCCTGCAGTCCGGCGACCATGCCCGGATTGCCCGCTGGCGGCGCTGGA
AGTCGTTGGTGCTCACCCATGAGCGCCGCCCGGACCTGTATGCCCGCGTGGTGCTGTCAAAGGCTGACCAGAAACTTCTG
GCCCGTCGGGAGGAAGAGCTGTAA

Upstream 100 bases:

>100_bases
CCGTGGACCTCGAGGCGCGGCGCATCGTCATCCATCCGCCCGAGTACGTGGAGGCGGGACGCAAGGGCGCCGAGTCAGGA
GGAGGCCCGGAGGACGCGGA

Downstream 100 bases:

>100_bases
CCCTGAACGTTGCTAGCGGGTTGGATCATCAGGGCTTGTCCGACACCGCTCTCTCTGTTAGTACGGCCCGCTCTTCACGC
GACCATCGCGTCAGTTCAAG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSPPYPVEILTLFPGMVTGYLGASILGKAQEKGLLATTITDIREYAEGKHRVTDDAPYGGGAGMVMKPEPLVAAIEAAKA
RHPGARVLLMSPRGPTFTQATARELVRHEAGLILVCGRYEGVDERVMAHLDGELSLGDFVLTGGELAAMTVVDAVARLVP
GVLGNVDSSVTESFEEGLLEHPQYTRPPVFRGVEVPAALQSGDHARIARWRRWKSLVLTHERRPDLYARVVLSKADQKLL
ARREEEL

Sequences:

>Translated_247_residues
MSPPYPVEILTLFPGMVTGYLGASILGKAQEKGLLATTITDIREYAEGKHRVTDDAPYGGGAGMVMKPEPLVAAIEAAKA
RHPGARVLLMSPRGPTFTQATARELVRHEAGLILVCGRYEGVDERVMAHLDGELSLGDFVLTGGELAAMTVVDAVARLVP
GVLGNVDSSVTESFEEGLLEHPQYTRPPVFRGVEVPAALQSGDHARIARWRRWKSLVLTHERRPDLYARVVLSKADQKLL
ARREEEL
>Mature_246_residues
SPPYPVEILTLFPGMVTGYLGASILGKAQEKGLLATTITDIREYAEGKHRVTDDAPYGGGAGMVMKPEPLVAAIEAAKAR
HPGARVLLMSPRGPTFTQATARELVRHEAGLILVCGRYEGVDERVMAHLDGELSLGDFVLTGGELAAMTVVDAVARLVPG
VLGNVDSSVTESFEEGLLEHPQYTRPPVFRGVEVPAALQSGDHARIARWRRWKSLVLTHERRPDLYARVVLSKADQKLLA
RREEEL

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=242, Percent_Identity=46.2809917355372, Blast_Score=197, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_MYXXD (Q1D6I2)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_631741.1
- ProteinModelPortal:   Q1D6I2
- SMR:   Q1D6I2
- STRING:   Q1D6I2
- GeneID:   4107604
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_3548
- TIGR:   MXAN_3548
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q1D6I2
- ProtClustDB:   PRK00026
- BioCyc:   MXAN246197:MXAN_3548-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26869; Mature: 26738

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPYPVEILTLFPGMVTGYLGASILGKAQEKGLLATTITDIREYAEGKHRVTDDAPYGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
GAGMVMKPEPLVAAIEAAKARHPGARVLLMSPRGPTFTQATARELVRHEAGLILVCGRYE
CCCCEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCC
GVDERVMAHLDGELSLGDFVLTGGELAAMTVVDAVARLVPGVLGNVDSSVTESFEEGLLE
CHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
HPQYTRPPVFRGVEVPAALQSGDHARIARWRRWKSLVLTHERRPDLYARVVLSKADQKLL
CCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ARREEEL
HHHHHCC
>Mature Secondary Structure 
SPPYPVEILTLFPGMVTGYLGASILGKAQEKGLLATTITDIREYAEGKHRVTDDAPYGG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
GAGMVMKPEPLVAAIEAAKARHPGARVLLMSPRGPTFTQATARELVRHEAGLILVCGRYE
CCCCEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCC
GVDERVMAHLDGELSLGDFVLTGGELAAMTVVDAVARLVPGVLGNVDSSVTESFEEGLLE
CHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
HPQYTRPPVFRGVEVPAALQSGDHARIARWRRWKSLVLTHERRPDLYARVVLSKADQKLL
CCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ARREEEL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA