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The map label for this gene is dosC [H]

Identifier: 108760999

GI number: 108760999

Start: 5525824

End: 5527197

Strand: Direct

Name: dosC [H]

Synonym: MXAN_4463

Alternate gene names: 108760999

Gene position: 5525824-5527197 (Clockwise)

Preceding gene: 108762025

Following gene: 108763801

Centisome position: 60.46

GC content: 69.51

Gene sequence:

>1374_bases
ATGGCGCGAATCCTCCTCGTCGACGACGAAAAGATCGCCCGCACCCTGTACGGCGACTACCTCACCGCCGTGGGACACGC
CGTCACGGCGGTGGGCACGCTACAAGAGGCAAAGGAAGCACTCGCAGGCGACCGTTTCGACGCGGTGGTGACGGACCTCA
TCCTCCCCGGTGGTGACGGCATGGAGGTCCTGCGGCACGTGCGGGAACATCACCCGGGCGTGGAGGTGGTGGTCATCACT
GGCCTGGAGAAGGTGGACCCCGCCGTGCGCGCCATCAAGAGCGGCGCCGCGGAGTACCTCGTCAAGCCGGTGGCCCCGGA
GGCCCTGCAGCACGCCGTGCGCCGAGCGCTCACCACGCGCGACCTGATGCAGGAGAACGCGTCGCTGCGCCGCCATGTGG
CCATGTTGGAGGCGGGGCAACGCATCGCCACCACCCTGGACCGCGAGAAGCTGGCCTCGGCCACCGCCAGCGCGCTGCAG
AGCATGGCCTCCGCCAGCGCCGTGGTCCTGCTGGAGCGCGACTCTGCCTTCGCGCTGCGGCGCCACGGCACCAGCGGCCT
GTCCACCGCGCTGGAAGAGCCGCTCATCGCCGAGCTCATCGAACGCCTGACGAACGAACGCGGTCCGCGCGAGCTGGACG
GCATGGACGCGCCCTTTCCTCGCGCAATCTCCTTCCCCGCGCTGGAGGGTGACGCCGTGCTGGGACACGCGGTGCTCTTC
TTCGGCGGCACGGGCGCGGAGTGGGCGGGCGAGACGGCCAGCTTCCTGGTTCGCAACTGGGCGCTCGCGCTGCGCAACCT
CGGCCGCTTCGCCGCGGTGGAGGACCTGGCGTACGTCGACGACCTCACGCGCCTGTTCAACACCCGCTACCTGCACCTGG
TGGTGGACCGCGAGGTCCAGGACGCGCTCCAGTCACAGCGCACCTTCAGCCTGCTGTTCCTGGACCTGGACCACTTCAAG
TCCATCAACGATACCCATGGCCACCTCGTGGGCTCCAAGGTGCTGGTGGAGGCGGCGCGCGTGGTGAAGGGCTGCGTGAG
AGACCACGACGTCGTCGCGCGCTACGGCGGAGACGAATACGTGGTGGTGCTGCGCAACACCGACTCCGGCGGCGCGCTCA
AGGTGGCCGAGCGCATCCGACGCACCATGGAGACGCACAACTTCCTGGCGCGCGAAGGCCTGTCGCTCAAGCTCACCACG
TGTATCGGCGTGGCCAGCTTCCCCGAGCACGCCCAGGACAAGGCCACGCTGTTGGACCTGTCGGACCGGGCCATGTACCG
CGGCAAGCGGGGCTCGCGGAACGTCGTCTACATGGCGGCGAAGGACCTGGAGGCCCCACCGGCCGAGCGCCGGCAGGCCC
ACTCCGCGTCCTGA

Upstream 100 bases:

>100_bases
CCGGCCTCAACGAGAAGGCAGAGCCGACGGCCGCTTTCGAAGGGGGACACGGGGGCCGACCCTAGCAGCCTTCCATCGGT
TGGTGCTAAGCCGATGGGAG

Downstream 100 bases:

>100_bases
CGGCTACCGGCGCAGGCTGCCCACGGTGAGGCGCTTCTCCGGGGTGACGTCCGCCGGAGACGGCGCCGCGCCCTCCTTGC
CCACGGCCTCCACGGCCTCG

Product: response regulator/GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MARILLVDDEKIARTLYGDYLTAVGHAVTAVGTLQEAKEALAGDRFDAVVTDLILPGGDGMEVLRHVREHHPGVEVVVIT
GLEKVDPAVRAIKSGAAEYLVKPVAPEALQHAVRRALTTRDLMQENASLRRHVAMLEAGQRIATTLDREKLASATASALQ
SMASASAVVLLERDSAFALRRHGTSGLSTALEEPLIAELIERLTNERGPRELDGMDAPFPRAISFPALEGDAVLGHAVLF
FGGTGAEWAGETASFLVRNWALALRNLGRFAAVEDLAYVDDLTRLFNTRYLHLVVDREVQDALQSQRTFSLLFLDLDHFK
SINDTHGHLVGSKVLVEAARVVKGCVRDHDVVARYGGDEYVVVLRNTDSGGALKVAERIRRTMETHNFLAREGLSLKLTT
CIGVASFPEHAQDKATLLDLSDRAMYRGKRGSRNVVYMAAKDLEAPPAERRQAHSAS

Sequences:

>Translated_457_residues
MARILLVDDEKIARTLYGDYLTAVGHAVTAVGTLQEAKEALAGDRFDAVVTDLILPGGDGMEVLRHVREHHPGVEVVVIT
GLEKVDPAVRAIKSGAAEYLVKPVAPEALQHAVRRALTTRDLMQENASLRRHVAMLEAGQRIATTLDREKLASATASALQ
SMASASAVVLLERDSAFALRRHGTSGLSTALEEPLIAELIERLTNERGPRELDGMDAPFPRAISFPALEGDAVLGHAVLF
FGGTGAEWAGETASFLVRNWALALRNLGRFAAVEDLAYVDDLTRLFNTRYLHLVVDREVQDALQSQRTFSLLFLDLDHFK
SINDTHGHLVGSKVLVEAARVVKGCVRDHDVVARYGGDEYVVVLRNTDSGGALKVAERIRRTMETHNFLAREGLSLKLTT
CIGVASFPEHAQDKATLLDLSDRAMYRGKRGSRNVVYMAAKDLEAPPAERRQAHSAS
>Mature_456_residues
ARILLVDDEKIARTLYGDYLTAVGHAVTAVGTLQEAKEALAGDRFDAVVTDLILPGGDGMEVLRHVREHHPGVEVVVITG
LEKVDPAVRAIKSGAAEYLVKPVAPEALQHAVRRALTTRDLMQENASLRRHVAMLEAGQRIATTLDREKLASATASALQS
MASASAVVLLERDSAFALRRHGTSGLSTALEEPLIAELIERLTNERGPRELDGMDAPFPRAISFPALEGDAVLGHAVLFF
GGTGAEWAGETASFLVRNWALALRNLGRFAAVEDLAYVDDLTRLFNTRYLHLVVDREVQDALQSQRTFSLLFLDLDHFKS
INDTHGHLVGSKVLVEAARVVKGCVRDHDVVARYGGDEYVVVLRNTDSGGALKVAERIRRTMETHNFLAREGLSLKLTTC
IGVASFPEHAQDKATLLDLSDRAMYRGKRGSRNVVYMAAKDLEAPPAERRQAHSAS

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=38.6075949367089, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082007, Length=195, Percent_Identity=32.8205128205128, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=31.055900621118, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=33.5365853658537, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787541, Length=159, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787802, Length=137, Percent_Identity=36.4963503649635, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=34.3949044585987, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=35.9281437125749, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789402, Length=143, Percent_Identity=35.6643356643357, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=29.2397660818713, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787056, Length=172, Percent_Identity=31.3953488372093, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=189, Percent_Identity=27.5132275132275, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=31.304347826087, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=33.6206896551724, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=31.5789473684211, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=26.7515923566879, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788956, Length=127, Percent_Identity=35.4330708661417, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87081977, Length=163, Percent_Identity=26.9938650306748, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788085, Length=157, Percent_Identity=28.0254777070064, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49846; Mature: 49715

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARILLVDDEKIARTLYGDYLTAVGHAVTAVGTLQEAKEALAGDRFDAVVTDLILPGGDG
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCH
MEVLRHVREHHPGVEVVVITGLEKVDPAVRAIKSGAAEYLVKPVAPEALQHAVRRALTTR
HHHHHHHHHHCCCCEEEEEECCHHHCHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHH
DLMQENASLRRHVAMLEAGQRIATTLDREKLASATASALQSMASASAVVLLERDSAFALR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHH
RHGTSGLSTALEEPLIAELIERLTNERGPRELDGMDAPFPRAISFPALEGDAVLGHAVLF
HCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEE
FGGTGAEWAGETASFLVRNWALALRNLGRFAAVEDLAYVDDLTRLFNTRYLHLVVDREVQ
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHH
DALQSQRTFSLLFLDLDHFKSINDTHGHLVGSKVLVEAARVVKGCVRDHDVVARYGGDEY
HHHHHCCCEEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCEE
VVVLRNTDSGGALKVAERIRRTMETHNFLAREGLSLKLTTCIGVASFPEHAQDKATLLDL
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCCHHEEEC
SDRAMYRGKRGSRNVVYMAAKDLEAPPAERRQAHSAS
CHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHCCCCC
>Mature Secondary Structure 
ARILLVDDEKIARTLYGDYLTAVGHAVTAVGTLQEAKEALAGDRFDAVVTDLILPGGDG
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCH
MEVLRHVREHHPGVEVVVITGLEKVDPAVRAIKSGAAEYLVKPVAPEALQHAVRRALTTR
HHHHHHHHHHCCCCEEEEEECCHHHCHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHH
DLMQENASLRRHVAMLEAGQRIATTLDREKLASATASALQSMASASAVVLLERDSAFALR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHH
RHGTSGLSTALEEPLIAELIERLTNERGPRELDGMDAPFPRAISFPALEGDAVLGHAVLF
HCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEE
FGGTGAEWAGETASFLVRNWALALRNLGRFAAVEDLAYVDDLTRLFNTRYLHLVVDREVQ
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHH
DALQSQRTFSLLFLDLDHFKSINDTHGHLVGSKVLVEAARVVKGCVRDHDVVARYGGDEY
HHHHHCCCEEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCEE
VVVLRNTDSGGALKVAERIRRTMETHNFLAREGLSLKLTTCIGVASFPEHAQDKATLLDL
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCCHHEEEC
SDRAMYRGKRGSRNVVYMAAKDLEAPPAERRQAHSAS
CHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]