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The map label for this gene is rlmN1

Identifier: 108760323

GI number: 108760323

Start: 1489549

End: 1490628

Strand: Reverse

Name: rlmN1

Synonym: MXAN_1275

Alternate gene names: 108760323

Gene position: 1490628-1489549 (Counterclockwise)

Preceding gene: 108758032

Following gene: 108759743

Centisome position: 16.31

GC content: 69.07

Gene sequence:

>1080_bases
ATGCCGTCCGACACGTCCGCCAACCTGTACGATTTGACGCGGCCCGCGCTGGGGGCGCTGCTCTCCGGTTGGGGCTTCGG
CCCCTACCACCGCGACCAGCTCTGGACCGCGCTGTACCGCCGGCACGCGACCACTTTCGACGAACTGGACGGCCTCAAGC
CGGAGCTGCTGCGCATGCTGCGCGAGCACACGCGCCTGGGCCAGTTGGCCACCCACCACGAGTCCTTCAGCAGCGACGGC
TTCACGCACAAGCTGCTGCTGCGACTGGACGACGGGCAGACCATTGAAACCGTGCTGATGCGGTTCAAGGGCCGCGCCAC
GGTGTGCATCAGCACGCAGGCCGGCTGCGCCATGGGCTGCGTCTTCTGCGCCACCGGGCAGATGGGGCTCTCACGCCACC
TGACGCCCGGCGAAATCGTGGGGCAGATACTCCACGTCAACCGCATCCTGCGCGCCTCCGGCGAGACGCTGCGCAACGTC
GTCCTCATGGGCATGGGCGAGCCGCTCCACAACTACGAGCACACGATGTCCGCGGTGGACGTGCTGGTGGATGCGCTGGG
GCTCGCCATGGGCCCACGCTTCATCACGCTCAGCACGGTGGGCGTCGTGCCCGGCATCCGGCGGCTCGCGGATGAAGAGC
GCCCCATCCACCTGGCCGTCAGCCTCCATGGCGCCACCGACGCCGAACGCGCGGCGCTGGTCCCCGCTGGACGCCGCTGG
CCCCTCGACGAGTTGATGGACGCGTGCCGCTACTACAGCGAGAAGCGCAAGCGCCGCATCTTCTTCGAGTGGACGCTCAT
CTCCGGCCGCAACGACACCGCCGAGCACGCGCACACGCTGGGTCAACTGCTGCGCGGCATGGACGCGCACGTCAACGTCA
TCCCCCTCAACCCCACGGTGGGTTACGACGGCGGCCCCAGCCGTCCGGAGTCCGTGCGCGCCTTCCAGGACGTGCTCGCC
ACCTATGACGTGCCCAGCACGGTGCGTCAGCGCCGGGGCATCGACATCGACGCGGGGTGTGGCCAGCTCAAGGCCACCGT
GGAACGGCGTTCACGCCGTTCACTTCCCACCAGCGCCTGA

Upstream 100 bases:

>100_bases
CGCCTGCCAGACCGCCCCGCAGGGCAGGGTCAATGTCCACGAATGGCACCGGGCACGGGTAGGTACGCCCGCGCCCGGTG
TGTTATCTCGGGGCCTGGTC

Downstream 100 bases:

>100_bases
CGCATTCCTGAAGGCCCCAAGCCTGCTGTCCGCCCAGGCCCCGGGTACGAGCGCACTGTCTCGAACGTCGAAGCGCCCGA
GCGCCGCGTGAGGAGAGCTC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase 1

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPSDTSANLYDLTRPALGALLSGWGFGPYHRDQLWTALYRRHATTFDELDGLKPELLRMLREHTRLGQLATHHESFSSDG
FTHKLLLRLDDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVNRILRASGETLRNV
VLMGMGEPLHNYEHTMSAVDVLVDALGLAMGPRFITLSTVGVVPGIRRLADEERPIHLAVSLHGATDAERAALVPAGRRW
PLDELMDACRYYSEKRKRRIFFEWTLISGRNDTAEHAHTLGQLLRGMDAHVNVIPLNPTVGYDGGPSRPESVRAFQDVLA
TYDVPSTVRQRRGIDIDAGCGQLKATVERRSRRSLPTSA

Sequences:

>Translated_359_residues
MPSDTSANLYDLTRPALGALLSGWGFGPYHRDQLWTALYRRHATTFDELDGLKPELLRMLREHTRLGQLATHHESFSSDG
FTHKLLLRLDDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVNRILRASGETLRNV
VLMGMGEPLHNYEHTMSAVDVLVDALGLAMGPRFITLSTVGVVPGIRRLADEERPIHLAVSLHGATDAERAALVPAGRRW
PLDELMDACRYYSEKRKRRIFFEWTLISGRNDTAEHAHTLGQLLRGMDAHVNVIPLNPTVGYDGGPSRPESVRAFQDVLA
TYDVPSTVRQRRGIDIDAGCGQLKATVERRSRRSLPTSA
>Mature_358_residues
PSDTSANLYDLTRPALGALLSGWGFGPYHRDQLWTALYRRHATTFDELDGLKPELLRMLREHTRLGQLATHHESFSSDGF
THKLLLRLDDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVNRILRASGETLRNVV
LMGMGEPLHNYEHTMSAVDVLVDALGLAMGPRFITLSTVGVVPGIRRLADEERPIHLAVSLHGATDAERAALVPAGRRWP
LDELMDACRYYSEKRKRRIFFEWTLISGRNDTAEHAHTLGQLLRGMDAHVNVIPLNPTVGYDGGPSRPESVRAFQDVLAT
YDVPSTVRQRRGIDIDAGCGQLKATVERRSRRSLPTSA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=360, Percent_Identity=36.3888888888889, Blast_Score=230, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN1_MYXXD (Q1DCU1)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_629532.1
- ProteinModelPortal:   Q1DCU1
- STRING:   Q1DCU1
- GeneID:   4103275
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_1275
- TIGR:   MXAN_1275
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   PFNPIGS
- PhylomeDB:   Q1DCU1
- ProtClustDB:   CLSK2315141
- BioCyc:   MXAN246197:MXAN_1275-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39740; Mature: 39609

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSDTSANLYDLTRPALGALLSGWGFGPYHRDQLWTALYRRHATTFDELDGLKPELLRML
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHH
REHTRLGQLATHHESFSSDGFTHKLLLRLDDGQTIETVLMRFKGRATVCISTQAGCAMGC
HHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHH
VFCATGQMGLSRHLTPGEIVGQILHVNRILRASGETLRNVVLMGMGEPLHNYEHTMSAVD
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHH
VLVDALGLAMGPRFITLSTVGVVPGIRRLADEERPIHLAVSLHGATDAERAALVPAGRRW
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCCCC
PLDELMDACRYYSEKRKRRIFFEWTLISGRNDTAEHAHTLGQLLRGMDAHVNVIPLNPTV
CHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCC
GYDGGPSRPESVRAFQDVLATYDVPSTVRQRRGIDIDAGCGQLKATVERRSRRSLPTSA
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PSDTSANLYDLTRPALGALLSGWGFGPYHRDQLWTALYRRHATTFDELDGLKPELLRML
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHH
REHTRLGQLATHHESFSSDGFTHKLLLRLDDGQTIETVLMRFKGRATVCISTQAGCAMGC
HHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHH
VFCATGQMGLSRHLTPGEIVGQILHVNRILRASGETLRNVVLMGMGEPLHNYEHTMSAVD
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHH
VLVDALGLAMGPRFITLSTVGVVPGIRRLADEERPIHLAVSLHGATDAERAALVPAGRRW
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCCCC
PLDELMDACRYYSEKRKRRIFFEWTLISGRNDTAEHAHTLGQLLRGMDAHVNVIPLNPTV
CHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCC
GYDGGPSRPESVRAFQDVLATYDVPSTVRQRRGIDIDAGCGQLKATVERRSRRSLPTSA
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA