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The map label for this gene is vsr [H]

Identifier: 108758434

GI number: 108758434

Start: 4195271

End: 4195819

Strand: Reverse

Name: vsr [H]

Synonym: MXAN_3599

Alternate gene names: 108758434

Gene position: 4195819-4195271 (Counterclockwise)

Preceding gene: 108761918

Following gene: 108763142

Centisome position: 45.91

GC content: 63.57

Gene sequence:

>549_bases
ATGGGCCTCTCCCGTTCCGAACAGATGTCGCGGATCCGCGGCCGAGACACCTCTCCCGAGCGTCTGCTCCGGTCGGCGCT
CTGGCGGGCCGGACTGCGCTTCCGCCTGCACACGCGAACTACTCATGGAAGGCCCGATGTGGTCTTCCCCAGGCACAAGG
TTGCTGTCTTCATCGATGGCTGCTTCTGGCATGGGTGCCCCAAGCACTATGTGCGCCCGCGAACGCGTGACGAGTTCTGG
TCCTCCAAGCTTCGAGAAAACGTCGAGCGGGACCGCCGACAAACGCTCCTCCTCGAAGAAGGTGGCTGGCGCGTCTGCCG
CTTCTGGGAGCACCAGATCTTCGAGTCCCTCGCGGAAGTAGTCGAGTGGGTCCGCCAAGCACTCCAGGACGGAGAATGGA
CTCCATCCGCATCGTGGAGGGTCGTCCAGGTCATCCCACAGGTCGGCGAGGGCGACATGGAGCAGCGCTGGATGGAGGAA
CTTCGAGACGCCACCGAGACTCGAACCGTGGTCGGACGACGGAGCACGAAGAAGTGGAAGAGGCCATGA

Upstream 100 bases:

>100_bases
CCGATCTCCCCGTCCACCCGTCGGGGCCCTGACGCAGTAGTGATACCGGGCCACCCATCAGTAGGAATGATACATAGAAC
CTGTTAGGTAGGGTCTACCA

Downstream 100 bases:

>100_bases
ACTCCGGGCCGAAGCAGCAGATCAAGAAGTGGGATGGCCGGAGACCCTACGGTATAAGTCCGGACGGCATGGCTCCAGGC
ATCAGGGCGAAGCTGGATAG

Product: very short patch repair protein

Products: DNA [C]

Alternate protein names: V.NaeI [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MGLSRSEQMSRIRGRDTSPERLLRSALWRAGLRFRLHTRTTHGRPDVVFPRHKVAVFIDGCFWHGCPKHYVRPRTRDEFW
SSKLRENVERDRRQTLLLEEGGWRVCRFWEHQIFESLAEVVEWVRQALQDGEWTPSASWRVVQVIPQVGEGDMEQRWMEE
LRDATETRTVVGRRSTKKWKRP

Sequences:

>Translated_182_residues
MGLSRSEQMSRIRGRDTSPERLLRSALWRAGLRFRLHTRTTHGRPDVVFPRHKVAVFIDGCFWHGCPKHYVRPRTRDEFW
SSKLRENVERDRRQTLLLEEGGWRVCRFWEHQIFESLAEVVEWVRQALQDGEWTPSASWRVVQVIPQVGEGDMEQRWMEE
LRDATETRTVVGRRSTKKWKRP
>Mature_181_residues
GLSRSEQMSRIRGRDTSPERLLRSALWRAGLRFRLHTRTTHGRPDVVFPRHKVAVFIDGCFWHGCPKHYVRPRTRDEFWS
SKLRENVERDRRQTLLLEEGGWRVCRFWEHQIFESLAEVVEWVRQALQDGEWTPSASWRVVQVIPQVGEGDMEQRWMEEL
RDATETRTVVGRRSTKKWKRP

Specific function: May nick NaeI sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=108, Percent_Identity=44.4444444444444, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 21823; Mature: 21692

Theoretical pI: Translated: 11.10; Mature: 11.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSRSEQMSRIRGRDTSPERLLRSALWRAGLRFRLHTRTTHGRPDVVFPRHKVAVFIDG
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCEECCCCEEEEEEEC
CFWHGCPKHYVRPRTRDEFWSSKLRENVERDRRQTLLLEEGGWRVCRFWEHQIFESLAEV
HHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHH
VEWVRQALQDGEWTPSASWRVVQVIPQVGEGDMEQRWMEELRDATETRTVVGRRSTKKWK
HHHHHHHHHCCCCCCCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RP
CC
>Mature Secondary Structure 
GLSRSEQMSRIRGRDTSPERLLRSALWRAGLRFRLHTRTTHGRPDVVFPRHKVAVFIDG
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCEECCCCEEEEEEEC
CFWHGCPKHYVRPRTRDEFWSSKLRENVERDRRQTLLLEEGGWRVCRFWEHQIFESLAEV
HHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHH
VEWVRQALQDGEWTPSASWRVVQVIPQVGEGDMEQRWMEELRDATETRTVVGRRSTKKWK
HHHHHHHHHCCCCCCCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7698663 [H]