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The map label for this gene is spo0F [H]

Identifier: 108757478

GI number: 108757478

Start: 1618308

End: 1618685

Strand: Reverse

Name: spo0F [H]

Synonym: MXAN_1378

Alternate gene names: 108757478

Gene position: 1618685-1618308 (Counterclockwise)

Preceding gene: 108756915

Following gene: 108758224

Centisome position: 17.71

GC content: 64.02

Gene sequence:

>378_bases
ATGTCCCACATCCTGGTCGTCGATGACGACGCGAGCCACCGCACGCTCATCTGCGATGCACTCGAGGAAATGGGCTATCC
CACGGTTCAAGCGGCCAACGGTCGTGAGGCGCTGGACCTGCTGGAGGGAGACATGCCCTCCGCCGTGCTGCTCGACTTGC
GGATGCCAGTCATGAGCGGTTGGGGATTGCTCGACGCACTCAAGAAGATGCCCCGTGCGCGGGGTCTGCCCATCATCATC
ATCTCCGGCTACGGCTTCGAATGGGAGGCCGAGCTCGTGGGCGCCGCGGGCTACATCTCCAAGCCCGTGGACCTGGACAA
GGTGCGGATGACGGTGCAGCAGATTGCCGGTCCGCCGGAGATGTCGCTCGTCCACTGA

Upstream 100 bases:

>100_bases
AAAGCACCGGGGCCCCTTCCGGAATGGAAGAGGAGTTCCCATCATCCTGCCCATAGAATTTCGCGGCGCCACACCCAGCC
GCAGCTTCCGAGGCCCTGCC

Downstream 100 bases:

>100_bases
GACGGCACCGTAGGAGCCAGCGGGCATCCCCCTGTTGACGGGTGGGGATTGTCCCCCGGTGCCGGGCTCGGGTGAGATGG
AGGACATTCCATGATGCCCT

Product: response regulator

Products: NA

Alternate protein names: Stage 0 sporulation protein F [H]

Number of amino acids: Translated: 125; Mature: 124

Protein sequence:

>125_residues
MSHILVVDDDASHRTLICDALEEMGYPTVQAANGREALDLLEGDMPSAVLLDLRMPVMSGWGLLDALKKMPRARGLPIII
ISGYGFEWEAELVGAAGYISKPVDLDKVRMTVQQIAGPPEMSLVH

Sequences:

>Translated_125_residues
MSHILVVDDDASHRTLICDALEEMGYPTVQAANGREALDLLEGDMPSAVLLDLRMPVMSGWGLLDALKKMPRARGLPIII
ISGYGFEWEAELVGAAGYISKPVDLDKVRMTVQQIAGPPEMSLVH
>Mature_124_residues
SHILVVDDDASHRTLICDALEEMGYPTVQAANGREALDLLEGDMPSAVLLDLRMPVMSGWGLLDALKKMPRARGLPIIII
SGYGFEWEAELVGAAGYISKPVDLDKVRMTVQQIAGPPEMSLVH

Specific function: Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=37.2881355932203, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=35.5371900826446, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13601; Mature: 13470

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHILVVDDDASHRTLICDALEEMGYPTVQAANGREALDLLEGDMPSAVLLDLRMPVMSG
CCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCEEEEEECCCHHCC
WGLLDALKKMPRARGLPIIIISGYGFEWEAELVGAAGYISKPVDLDKVRMTVQQIAGPPE
CCHHHHHHHCHHHCCCCEEEEECCCCEEEHEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
MSLVH
CCCCC
>Mature Secondary Structure 
SHILVVDDDASHRTLICDALEEMGYPTVQAANGREALDLLEGDMPSAVLLDLRMPVMSG
CEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCEEEEEECCCHHCC
WGLLDALKKMPRARGLPIIIISGYGFEWEAELVGAAGYISKPVDLDKVRMTVQQIAGPPE
CCHHHHHHHCHHHCCCCEEEEECCCCEEEHEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
MSLVH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7514590 [H]