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The map label for this gene is IS1086 [H]

Identifier: 108756774

GI number: 108756774

Start: 1546180

End: 1547382

Strand: Reverse

Name: IS1086 [H]

Synonym: MXAN_1322

Alternate gene names: 108756774

Gene position: 1547382-1546180 (Counterclockwise)

Preceding gene: 108761603

Following gene: 108759446

Centisome position: 16.93

GC content: 70.24

Gene sequence:

>1203_bases
ATGCGCGGAAGGGAGCGCATCCGGGAGAGGTGCCCGGTGGAGAGTCGAGGCAAGCTCGGCAGGATGAGTGGAGAGGATTG
GCTGGAGGTGCGGCGCGCGGTGGCCGCCGGACAGACGCAGGAGGAGGCAGCGCGGGCGGCAGGGGTGAATGAGCGCACGC
TGCAGCGCCTTTTGCGCCGTGCGGGCGGCATCCGGCGGCCTGCGCTCGTGCGCTCTACCTTGCGTTTGAGCGTGGCCGAG
CGCGAGGAGGTGTCTCGCGGTGTGCAGGCCGGCGAGTCCTTGCGGGCCATCGCGCGGCGACTGGGGCGTGCTGCGTCCAC
CATCAGCCGCGAAGTGGGGCGTGCCGGCAAGGGACGCGAAGGCTACCGGGCGTGGCTGGGTGAAGCGGCGGCCGCGAGGG
CGTCGCGCCGGCCCAAGCAAACGAAGCTGTTGGCCCAGCCCCAGTTGCGCCAGGAGGTGGAGCGACGTCTGCTGCGGTGC
TGGAGTCCCCAGCAGATTGCCGCCACGCTCAAGCGCGACTTCGCCCACCAGCCCCAGATGCACGTGTCCCACGAAACCAT
TTACCGCAGCCTCTACGTGCAGACGCGGGGCAGCCTGCGCAAGGAACTCGCCGCGTACCTGCGCACGGGCCGCCCCCAGC
GTCGGCCCCGTGGCCGCGCCAGCGTCCTGTCCGGCCAGTTGCCGGGCCGGGTGATGCTCAGTGAGCGCCCGCCGGAAGCC
GAAAGCCGCGCCGTCCCGGGCCACTGGGAGGGCGACTTGCTGGTAGGCAAGCAGGGCAAGAGCGCCATCGGCACGCTGGT
GGAGCGCCGCAGCCGGTACGTCATGCTGTTGCACCTGCCCGAGGGGCGCACCGCGCCCCACGTGCGTGAGGCCCTCACCC
ACCAACTTGGCCAACTGCCTGACGCCTTGCGCCGCACCCTCACCTGGGACCAAGGCAAGGAGATGGCCGAGCACGTGCGC
TTCACCATCGACACCGACGTCCAAGTCTACTTCTGCGACCCCCACAGCCCCTGGCAACGCGGTAGCAACGAGAACACCAA
CGGCCTGCTGCGCCAGTACTTCCCCAAGGGCATGGATTTGAGCGGCCTGTCGCGCGCCCAGCTCGACACCGTCGCCCTCC
AACTCAACACCCGCCCCCGCCAGACGCTCGACTGGCGCACCCCCGCCGAGGTATTCGCTCAAAGCGTTGCGATGACCGGC
TGA

Upstream 100 bases:

>100_bases
GCTTGGGCGCAGCGCCCCCTTGGAGGCCGTTCGCGAGCTCGCTGTTCGGAAATGTCGGGACATTTGCCGGCGGTCTCAAT
CGGTGAACGCACCAGTCAGG

Downstream 100 bases:

>100_bases
AACCGCCGCCGCTTCGAAACGCAATGCGTGGCACCTCCGCGCCCGCCGAACGGGGACGGGGCGAGGAGGCACGACAGCAC
GCCCGATGCGCGGCGTGGGG

Product: IS30 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRGRERIRERCPVESRGKLGRMSGEDWLEVRRAVAAGQTQEEAARAAGVNERTLQRLLRRAGGIRRPALVRSTLRLSVAE
REEVSRGVQAGESLRAIARRLGRAASTISREVGRAGKGREGYRAWLGEAAAARASRRPKQTKLLAQPQLRQEVERRLLRC
WSPQQIAATLKRDFAHQPQMHVSHETIYRSLYVQTRGSLRKELAAYLRTGRPQRRPRGRASVLSGQLPGRVMLSERPPEA
ESRAVPGHWEGDLLVGKQGKSAIGTLVERRSRYVMLLHLPEGRTAPHVREALTHQLGQLPDALRRTLTWDQGKEMAEHVR
FTIDTDVQVYFCDPHSPWQRGSNENTNGLLRQYFPKGMDLSGLSRAQLDTVALQLNTRPRQTLDWRTPAEVFAQSVAMTG

Sequences:

>Translated_400_residues
MRGRERIRERCPVESRGKLGRMSGEDWLEVRRAVAAGQTQEEAARAAGVNERTLQRLLRRAGGIRRPALVRSTLRLSVAE
REEVSRGVQAGESLRAIARRLGRAASTISREVGRAGKGREGYRAWLGEAAAARASRRPKQTKLLAQPQLRQEVERRLLRC
WSPQQIAATLKRDFAHQPQMHVSHETIYRSLYVQTRGSLRKELAAYLRTGRPQRRPRGRASVLSGQLPGRVMLSERPPEA
ESRAVPGHWEGDLLVGKQGKSAIGTLVERRSRYVMLLHLPEGRTAPHVREALTHQLGQLPDALRRTLTWDQGKEMAEHVR
FTIDTDVQVYFCDPHSPWQRGSNENTNGLLRQYFPKGMDLSGLSRAQLDTVALQLNTRPRQTLDWRTPAEVFAQSVAMTG
>Mature_400_residues
MRGRERIRERCPVESRGKLGRMSGEDWLEVRRAVAAGQTQEEAARAAGVNERTLQRLLRRAGGIRRPALVRSTLRLSVAE
REEVSRGVQAGESLRAIARRLGRAASTISREVGRAGKGREGYRAWLGEAAAARASRRPKQTKLLAQPQLRQEVERRLLRC
WSPQQIAATLKRDFAHQPQMHVSHETIYRSLYVQTRGSLRKELAAYLRTGRPQRRPRGRASVLSGQLPGRVMLSERPPEA
ESRAVPGHWEGDLLVGKQGKSAIGTLVERRSRYVMLLHLPEGRTAPHVREALTHQLGQLPDALRRTLTWDQGKEMAEHVR
FTIDTDVQVYFCDPHSPWQRGSNENTNGLLRQYFPKGMDLSGLSRAQLDTVALQLNTRPRQTLDWRTPAEVFAQSVAMTG

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790736, Length=371, Percent_Identity=42.8571428571429, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1787671, Length=371, Percent_Identity=42.8571428571429, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1786450, Length=371, Percent_Identity=42.5876010781671, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 45160; Mature: 45160

Theoretical pI: Translated: 11.83; Mature: 11.83

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGRERIRERCPVESRGKLGRMSGEDWLEVRRAVAAGQTQEEAARAAGVNERTLQRLLRR
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHH
AGGIRRPALVRSTLRLSVAEREEVSRGVQAGESLRAIARRLGRAASTISREVGRAGKGRE
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
GYRAWLGEAAAARASRRPKQTKLLAQPQLRQEVERRLLRCWSPQQIAATLKRDFAHQPQM
HHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCH
HVSHETIYRSLYVQTRGSLRKELAAYLRTGRPQRRPRGRASVLSGQLPGRVMLSERPPEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCH
ESRAVPGHWEGDLLVGKQGKSAIGTLVERRSRYVMLLHLPEGRTAPHVREALTHQLGQLP
HCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCH
DALRRTLTWDQGKEMAEHVRFTIDTDVQVYFCDPHSPWQRGSNENTNGLLRQYFPKGMDL
HHHHHHCCHHHHHHHHHHCEEEEECCEEEEEECCCCHHHCCCCCCHHHHHHHHCCCCCCC
SGLSRAQLDTVALQLNTRPRQTLDWRTPAEVFAQSVAMTG
CCCCHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRGRERIRERCPVESRGKLGRMSGEDWLEVRRAVAAGQTQEEAARAAGVNERTLQRLLRR
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHH
AGGIRRPALVRSTLRLSVAEREEVSRGVQAGESLRAIARRLGRAASTISREVGRAGKGRE
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
GYRAWLGEAAAARASRRPKQTKLLAQPQLRQEVERRLLRCWSPQQIAATLKRDFAHQPQM
HHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCH
HVSHETIYRSLYVQTRGSLRKELAAYLRTGRPQRRPRGRASVLSGQLPGRVMLSERPPEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCH
ESRAVPGHWEGDLLVGKQGKSAIGTLVERRSRYVMLLHLPEGRTAPHVREALTHQLGQLP
HCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCH
DALRRTLTWDQGKEMAEHVRFTIDTDVQVYFCDPHSPWQRGSNENTNGLLRQYFPKGMDL
HHHHHHCCHHHHHHHHHHCEEEEECCEEEEEECCCCHHHCCCCCCHHHHHHHHCCCCCCC
SGLSRAQLDTVALQLNTRPRQTLDWRTPAEVFAQSVAMTG
CCCCHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1334071 [H]