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The map label for this gene is alaS [H]

Identifier: 108563608

GI number: 108563608

Start: 1246325

End: 1248868

Strand: Reverse

Name: alaS [H]

Synonym: HPAG1_1183

Alternate gene names: 108563608

Gene position: 1248868-1246325 (Counterclockwise)

Preceding gene: 108563610

Following gene: 108563607

Centisome position: 78.23

GC content: 42.53

Gene sequence:

>2544_bases
ATGGATATTCGCAACGAATTTTTACAATTTTTTCAAAATAAAGGGCATGCCGTTTATCCTAGCATGCCTTTAGTGCCTAA
TGACGCTACCTTGCTTTTTACCAATGCTGGCATGGTGCAATTTAAAGATATTTTTACCGGTATAGTGCCGCACCCTAGCA
TTCCCAGAGCGACAAGCTCGCAATTGTGCATGCGTGCAGGCGGCAAGCATAACGATTTAGAAAACGTCGGTTATACCGCA
AGGCACCACACGCTTTTTGAAATGCTAGGCAATTTCTCTTTTGGGGATTATTTCAAAGAAGAAGCGATTTTGTTTGCGTG
GGAATTTGTAACCAAAAATTTAGGGTTTAAGCCTAAAGATTTATACATCAGCGTGCATGAAAAAGACGATGAAGCCGTTA
AATTATGGGAAAAGTTTGTGCCTGTTGACAGGATTAAAAAAATGGGCGATAAAGATAATTTCTGGCAAATGGGCGATAGC
GGGCCTTGCGGGCCTTGCAGTGAAATTTATATTGATCAGGGCGAAAAACACTTTAAAGGGAGCGAGGATTATTTTGGGGG
CGAGGGCGATAGGTTTTTAGAAATTTGGAATTTGGTGTTCATGCAATACGAACGCTCTAATGATGGCGTTTTATCCCCCT
TGCCAAAGCCCAGCATTGATACTGGCATGGGATTAGAAAGGGTGCAAGCGCTATTAGAACATAAGCTCAATAATTTTGAT
TCTTCATTATTTGCGCCCCTAATGGAAGAAATCAGCGAGCTTACAAGCCTGGATTATACGAGCGAGTTCCAGCCAAGCTT
TAGGGTAGTGGCCGATCACGCAAGGGCGGTAGCGTTTTTGCTCGCTCAAGGGGTGCATTTCAATAAGGAAGGCCGTGGCT
ATGTTTTAAGGCGTATTTTAAGGCGAGCCTTAAGGCATGGGTATTTAATGGGCTTGAAAGAAGCGTTTTTATACAAAGTC
GTGGGCGTGGTGTGCGAGCAATTTTCTAACACGCATGCGTATTTGAAAGAGTCTAAAGAAATGGTGATGAAAGAATGCTT
TGAAGAAGAAGAGCGCTTTTTAGAGACTTTGGAATCTGGCATGGAATTGTTTAACTTGTCTTTAAAGCATTTGAATGAAA
ATAAGATTTTTGATGGCAAGATCGCTTTCAAGCTTTATGACACTTTTGGTTTTCCTTTGGATTTAACAAACGATATGCTA
AGAAGTCATGGGGCGTGCGTGGATATGCAAGGCTTTGAATTGTGCATGCAAGAGCAAGTGAAACGCTCTAAAGCTTCATG
GAAAGGCAAACAAGATAACGCTGATTTTAGCGCTATTTTAAACGCTTATGCGCCCAACGAATTTGTAGGGTATGAAACGA
CAGAATGTCCTGCTAAAGCCTTAGGGTTTTTTGATAGCGATTTTAAAGAAATAATAGAGGCAAATCCTAACCAAGAAGTC
TGGGTGTTGTTAGAAAAAACCCCTTTTTATGCAGAAGGTGGGGGGGCTATAGGCGATAGGGGCGCGCTTTTTAAAGACAA
TGAAGAAGCGGCTATAGTGCTAGATACAAAAAACTTTTTTGGGCTTAATTTTTCGCTCCTTGAAATCAAAAAAGCGCTAA
AAAAAGGCGATCAAGTGATCGCGCAAGTGAGCGATGAGCGCTTAGAAATCGCCAAACACCATAGCGCGACGCATTTATTA
CAGAGCGCTTTAAGAGAAGTTTTAGGCTCGCATGTGAGTCAAGCGGGGAGTTTAGTGGAATCCAAACGATTGCGCTTTGA
TTTCTCGCACCCTAAAGTGCTCAATGATGAAGAGCTAGAAAAAGTAGAAGATTTAGTTAATGCTCAAATTTTCAAGCACC
TAAATAGCCATGTGGAGCATATGCCCTTAAACCAAGCCAAAGATAAGGGAGCGTTAGCGTTATTTGGTGAAAAATACGCT
GAAAATGTGCGGGTGGTGAGCTTTAAAGAAGCGTCCATTGAATTGTGTGGGGGCATTCATGTGGAAAATACCGGGCTGAT
TGGGGGGTTTAGGATTGTAAAAGAAAGCGGGGTGAGTAGTGGGGTCAGACGCATTGAAGCGGTGTGCGGGAAAGCCTTTT
ACCAACTGGCTAAAGAAGAAAGTAAAGAACTTAAAAACGCTAAGACTTTATTGAAAAATAACGATGTCATAGCCGGCATC
AACAAGCTTAAAGAGAGCGTGAAAAACAGCCAAAAAGCCCCCGTTTCTGTGGATTTACCGGTTGAAAAAATCCATGGCGT
GAATTTGGTGGTGGGCGTCGTGGAACAAGGCGACATTAAAGAAATGATTGACCGATTGAAAAGTAAGCATGAAAGATTGC
TCGCTATGGTGTTTAAAAAAGAAAACGAGCGAATCACTCTCGCATGCGGGGTGAAAAACGCGCCCATAAAAGCGAATGTG
TGGGCTAATGAAGTGGCACAAATTTTAGGGGGCAAAGGGGGCGGGAGAGATGATTTTGCGAGCGCTGGAGGTAAGGATAT
TGAAAATTTGCAAGCCGCGCTCAATTTAGCGAAAAATACCGCTCTTAAAGCTTTAGAGGGATAG

Upstream 100 bases:

>100_bases
TGGATGTTTAAAGTATAATAAACTATCTTTTTAAAAAAACAACTTAAAAGAGCTATAAATAGCCTTAAAATGCGCGATAA
AACAACAAAAAGGAATACCC

Downstream 100 bases:

>100_bases
CATGGAGCTTATTTTAGGCTCTCAATCTAGCGCTAGGGCGAATCTTTTAAAAGAGCATGGGATTAAGTTTGAACAAAAAG
CACTCTATTTTGATGAAGAA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 847; Mature: 847

Protein sequence:

>847_residues
MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPHPSIPRATSSQLCMRAGGKHNDLENVGYTA
RHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKDLYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDS
GPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
SSLFAPLMEEISELTSLDYTSEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKV
VGVVCEQFSNTHAYLKESKEMVMKECFEEEERFLETLESGMELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDML
RSHGACVDMQGFELCMQEQVKRSKASWKGKQDNADFSAILNAYAPNEFVGYETTECPAKALGFFDSDFKEIIEANPNQEV
WVLLEKTPFYAEGGGAIGDRGALFKDNEEAAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERLEIAKHHSATHLL
QSALREVLGSHVSQAGSLVESKRLRFDFSHPKVLNDEELEKVEDLVNAQIFKHLNSHVEHMPLNQAKDKGALALFGEKYA
ENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEESKELKNAKTLLKNNDVIAGI
NKLKESVKNSQKAPVSVDLPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKKENERITLACGVKNAPIKANV
WANEVAQILGGKGGGRDDFASAGGKDIENLQAALNLAKNTALKALEG

Sequences:

>Translated_847_residues
MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPHPSIPRATSSQLCMRAGGKHNDLENVGYTA
RHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKDLYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDS
GPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
SSLFAPLMEEISELTSLDYTSEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKV
VGVVCEQFSNTHAYLKESKEMVMKECFEEEERFLETLESGMELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDML
RSHGACVDMQGFELCMQEQVKRSKASWKGKQDNADFSAILNAYAPNEFVGYETTECPAKALGFFDSDFKEIIEANPNQEV
WVLLEKTPFYAEGGGAIGDRGALFKDNEEAAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERLEIAKHHSATHLL
QSALREVLGSHVSQAGSLVESKRLRFDFSHPKVLNDEELEKVEDLVNAQIFKHLNSHVEHMPLNQAKDKGALALFGEKYA
ENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEESKELKNAKTLLKNNDVIAGI
NKLKESVKNSQKAPVSVDLPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKKENERITLACGVKNAPIKANV
WANEVAQILGGKGGGRDDFASAGGKDIENLQAALNLAKNTALKALEG
>Mature_847_residues
MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPHPSIPRATSSQLCMRAGGKHNDLENVGYTA
RHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKDLYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDS
GPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
SSLFAPLMEEISELTSLDYTSEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKV
VGVVCEQFSNTHAYLKESKEMVMKECFEEEERFLETLESGMELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDML
RSHGACVDMQGFELCMQEQVKRSKASWKGKQDNADFSAILNAYAPNEFVGYETTECPAKALGFFDSDFKEIIEANPNQEV
WVLLEKTPFYAEGGGAIGDRGALFKDNEEAAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERLEIAKHHSATHLL
QSALREVLGSHVSQAGSLVESKRLRFDFSHPKVLNDEELEKVEDLVNAQIFKHLNSHVEHMPLNQAKDKGALALFGEKYA
ENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEESKELKNAKTLLKNNDVIAGI
NKLKESVKNSQKAPVSVDLPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKKENERITLACGVKNAPIKANV
WANEVAQILGGKGGGRDDFASAGGKDIENLQAALNLAKNTALKALEG

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=963, Percent_Identity=35.9293873312565, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI38569417, Length=954, Percent_Identity=31.7610062893082, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1789048, Length=863, Percent_Identity=46.8134414831981, Blast_Score=702, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=970, Percent_Identity=34.020618556701, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17536681, Length=741, Percent_Identity=32.6585695006748, Blast_Score=333, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6324911, Length=771, Percent_Identity=40.337224383917, Blast_Score=501, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24582809, Length=965, Percent_Identity=32.7461139896373, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI45552267, Length=965, Percent_Identity=32.7461139896373, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24658214, Length=794, Percent_Identity=29.7229219143577, Blast_Score=291, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 94918; Mature: 94918

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPHPSIPRATSS
CCHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCHH
QLCMRAGGKHNDLENVGYTARHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKD
HHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCE
LYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDSGPCGPCSEIYIDQGEKHFKG
EEEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCEECCCCCCCCCCCHHHCCCCCHHCCC
SEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
CCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
SSLFAPLMEEISELTSLDYTSEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRIL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RRALRHGYLMGLKEAFLYKVVGVVCEQFSNTHAYLKESKEMVMKECFEEEERFLETLESG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDMLRSHGACVDMQGFELCMQEQV
HHHHHHHHHHCCCCCEECCEEEEEEEECCCCCCCCHHHHHHCCCCEECCHHHHHHHHHHH
KRSKASWKGKQDNADFSAILNAYAPNEFVGYETTECPAKALGFFDSDFKEIIEANPNQEV
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCEE
WVLLEKTPFYAEGGGAIGDRGALFKDNEEAAIVLDTKNFFGLNFSLLEIKKALKKGDQVI
EEEEECCCCEECCCCCCCCCCCCEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
AQVSDERLEIAKHHSATHLLQSALREVLGSHVSQAGSLVESKRLRFDFSHPKVLNDEELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHH
KVEDLVNAQIFKHLNSHVEHMPLNQAKDKGALALFGEKYAENVRVVSFKEASIELCGGIH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHCCCEEEEEECCCHHHHCCEE
VENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEESKELKNAKTLLKNNDVIAGI
ECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECH
NKLKESVKNSQKAPVSVDLPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKK
HHHHHHHHCCCCCCEEECCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
ENERITLACGVKNAPIKANVWANEVAQILGGKGGGRDDFASAGGKDIENLQAALNLAKNT
CCCEEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHH
ALKALEG
HHHHCCC
>Mature Secondary Structure
MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPHPSIPRATSS
CCHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCHH
QLCMRAGGKHNDLENVGYTARHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKD
HHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCE
LYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDSGPCGPCSEIYIDQGEKHFKG
EEEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCEECCCCCCCCCCCHHHCCCCCHHCCC
SEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
CCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
SSLFAPLMEEISELTSLDYTSEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRIL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RRALRHGYLMGLKEAFLYKVVGVVCEQFSNTHAYLKESKEMVMKECFEEEERFLETLESG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDMLRSHGACVDMQGFELCMQEQV
HHHHHHHHHHCCCCCEECCEEEEEEEECCCCCCCCHHHHHHCCCCEECCHHHHHHHHHHH
KRSKASWKGKQDNADFSAILNAYAPNEFVGYETTECPAKALGFFDSDFKEIIEANPNQEV
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCEE
WVLLEKTPFYAEGGGAIGDRGALFKDNEEAAIVLDTKNFFGLNFSLLEIKKALKKGDQVI
EEEEECCCCEECCCCCCCCCCCCEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
AQVSDERLEIAKHHSATHLLQSALREVLGSHVSQAGSLVESKRLRFDFSHPKVLNDEELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHH
KVEDLVNAQIFKHLNSHVEHMPLNQAKDKGALALFGEKYAENVRVVSFKEASIELCGGIH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHCCCEEEEEECCCHHHHCCEE
VENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEESKELKNAKTLLKNNDVIAGI
ECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECH
NKLKESVKNSQKAPVSVDLPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKK
HHHHHHHHCCCCCCEEECCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
ENERITLACGVKNAPIKANVWANEVAQILGGKGGGRDDFASAGGKDIENLQAALNLAKNT
CCCEEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHH
ALKALEG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA