BacMap

Definition	Helicobacter pylori HPAG1 chromosome, complete genome.
Accession	NC_008086
Length	1,596,366

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The map label for this gene is tuf [H]

Identifier: 108563571

GI number: 108563571

Start: 1212092

End: 1212817

Strand: Reverse

Name: tuf [H]

Synonym: HPAG1_1146

Alternate gene names: 108563571

Gene position: 1212817-1212092 (Counterclockwise)

Preceding gene: 108563573

Following gene: 108563570

Centisome position: 75.97

GC content: 43.66

Gene sequence:

>726_bases
ATGGCAAAAGAAAAGTTTAATAGAACTAAGCCGCATGTTAATATTGGAACCATTGGGCATGTAGACCATGGTAAAACGAC
TTTGAGTGCAGCGATTTCAGCGGTGCTTTCTTTGAAAGGTCTTGCAGAAATGAAAGACTATGATAATATTGATAACGCCC
CCGAAGAAAAAGAAAGAGGGATCACTATCGCTACTTCTCACATTGAATATGAGACTGAAAACAGACACTATGCGCATGTG
GATTGCCCAGGACACGCTGACTATGTAAAAAACATGATTACCGGTGCGGCGCAAATGGACGGAGCGATTTTGGTTGTTTC
TGCAGCTGATGGTCCTATGCCTCAAACCAGAGAGCATATCTTATTGTCTCGTCAAGTAGGCGTGCCTCACATCGTTGTTT
TCTTAAACAAACAAGACATGGTAGATGACCAAGAATTGTTAGAGCTTGTAGAAATGGAAGTACGCGAATTGTTGAGCGCG
TATGAATTCCCTGGCGATGACACTCCTATCATAGCGGGTTCAGCTTTAAGAGCTTTAGAAGAAGCAAAGGCTGGCAATGT
GGGTGAATGGGGTGAAAAAGTGCTTAAGCTCATGGCTGAAGTGGATGCCTATATCCCTACTCCAGAAAGAGACACTGAAA
AAACTTTCTTGATGCCGGTGAAGATGTGTTCTCTATTGCGGGTAGAGGGACTGTGGTTACAGGTAGGATTGAAAGAGGTG
TGGTGA

Upstream 100 bases:

>100_bases
GACAGAAATGTCTTAAATTTTGTGGTAGCATTTAGGAATACTTAGGGTTTGTTTAGTATAATTCTAAAATCCATTTCAAT
AAAATTAAGGAGAAATACAA

Downstream 100 bases:

>100_bases
AAGTAGGCGATGAAGTGGAAATCGTTGGTATTAGAGCTACACAAAAAACGACTGTAACCGGTGTAGAAATGTTTAGAAAA
GAGTTAGAAAAAGGTGAGGC

Product: translation elongation factor EF-Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MAKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERGITIATSHIEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKQDMVDDQELLELVEMEVRELLSA
YEFPGDDTPIIAGSALRALEEAKAGNVGEWGEKVLKLMAEVDAYIPTPERDTEKTFLMPVKMCSLLRVEGLWLQVGLKEV
W

Sequences:

>Translated_241_residues
MAKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERGITIATSHIEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKQDMVDDQELLELVEMEVRELLSA
YEFPGDDTPIIAGSALRALEEAKAGNVGEWGEKVLKLMAEVDAYIPTPERDTEKTFLMPVKMCSLLRVEGLWLQVGLKEV
W
>Mature_240_residues
AKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERGITIATSHIEYETENRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKQDMVDDQELLELVEMEVRELLSAY
EFPGDDTPIIAGSALRALEEAKAGNVGEWGEKVLKLMAEVDAYIPTPERDTEKTFLMPVKMCSLLRVEGLWLQVGLKEVW

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=221, Percent_Identity=62.89592760181, Blast_Score=288, Evalue=3e-78,
Organism=Homo sapiens, GI223555963, Length=268, Percent_Identity=30.9701492537313, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI5729864, Length=268, Percent_Identity=30.9701492537313, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI46094014, Length=152, Percent_Identity=35.5263157894737, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI194018522, Length=152, Percent_Identity=35.5263157894737, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI194097354, Length=152, Percent_Identity=35.5263157894737, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI194018520, Length=152, Percent_Identity=35.5263157894737, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4503471, Length=254, Percent_Identity=29.9212598425197, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4503475, Length=180, Percent_Identity=35, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=34.8148148148148, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1790412, Length=221, Percent_Identity=72.3981900452489, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1789737, Length=221, Percent_Identity=72.3981900452489, Blast_Score=305, Evalue=1e-84,
Organism=Escherichia coli, GI2367247, Length=218, Percent_Identity=33.4862385321101, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789108, Length=158, Percent_Identity=34.8101265822785, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994988, Length=219, Percent_Identity=32.4200913242009, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790835, Length=151, Percent_Identity=30.4635761589404, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=133, Percent_Identity=32.3308270676692, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=212, Percent_Identity=62.2641509433962, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI25141371, Length=211, Percent_Identity=51.1848341232227, Blast_Score=224, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI32566629, Length=258, Percent_Identity=29.4573643410853, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115532067, Length=152, Percent_Identity=36.8421052631579, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI115532065, Length=152, Percent_Identity=36.8421052631579, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17552884, Length=264, Percent_Identity=28.7878787878788, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17569207, Length=264, Percent_Identity=28.7878787878788, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17506081, Length=227, Percent_Identity=27.7533039647577, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566301, Length=156, Percent_Identity=34.6153846153846, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI32566303, Length=125, Percent_Identity=34.4, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=217, Percent_Identity=69.5852534562212, Blast_Score=311, Evalue=6e-86,
Organism=Saccharomyces cerevisiae, GI6325337, Length=251, Percent_Identity=29.4820717131474, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319594, Length=251, Percent_Identity=29.4820717131474, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322937, Length=154, Percent_Identity=30.5194805194805, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=173, Percent_Identity=31.2138728323699, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320377, Length=140, Percent_Identity=36.4285714285714, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.8148148148148, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363316, Length=219, Percent_Identity=63.9269406392694, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI17864358, Length=219, Percent_Identity=63.9269406392694, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI19921738, Length=214, Percent_Identity=61.6822429906542, Blast_Score=275, Evalue=1e-74,
Organism=Drosophila melanogaster, GI17137380, Length=248, Percent_Identity=31.0483870967742, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI45553807, Length=268, Percent_Identity=27.9850746268657, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI45553816, Length=268, Percent_Identity=27.9850746268657, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24651721, Length=268, Percent_Identity=27.9850746268657, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17864154, Length=268, Percent_Identity=27.9850746268657, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24652838, Length=185, Percent_Identity=33.5135135135135, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17137572, Length=185, Percent_Identity=33.5135135135135, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922690, Length=232, Percent_Identity=31.8965517241379, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550900, Length=239, Percent_Identity=29.7071129707113, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24647046, Length=201, Percent_Identity=29.8507462686567, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24647048, Length=201, Percent_Identity=29.8507462686567, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=36.3636363636364, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 26701; Mature: 26570

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERG
CCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
ITIATSHIEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPHIVVFLNKQDMVDDQELLELVEMEVRELLSAYEFPGDDTPIIAGSALRALE
HHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHH
EAKAGNVGEWGEKVLKLMAEVDAYIPTPERDTEKTFLMPVKMCSLLRVEGLWLQVGLKEV
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECHHHC
W
C
>Mature Secondary Structure 
AKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERG
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
ITIATSHIEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPHIVVFLNKQDMVDDQELLELVEMEVRELLSAYEFPGDDTPIIAGSALRALE
HHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHH
EAKAGNVGEWGEKVLKLMAEVDAYIPTPERDTEKTFLMPVKMCSLLRVEGLWLQVGLKEV
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECHHHC
W
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA