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The map label for this gene is tuf [H]

Identifier: 108563570

GI number: 108563570

Start: 1211619

End: 1212152

Strand: Reverse

Name: tuf [H]

Synonym: HPAG1_1145

Alternate gene names: 108563570

Gene position: 1212152-1211619 (Counterclockwise)

Preceding gene: 108563571

Following gene: 108563569

Centisome position: 75.93

GC content: 44.94

Gene sequence:

>534_bases
GTGTTCTCTATTGCGGGTAGAGGGACTGTGGTTACAGGTAGGATTGAAAGAGGTGTGGTGAAAGTAGGCGATGAAGTGGA
AATCGTTGGTATTAGAGCTACACAAAAAACGACTGTAACCGGTGTAGAAATGTTTAGAAAAGAGTTAGAAAAAGGTGAGG
CCGGCGATAATGTGGGCGTGCTTTTGAGAGGAACTAAAAAAGAAGAAGTAGAGCGCGGTATGGTTCTATGCAAACCAGGT
TCTATCACTCCGCACAAGAAATTTGAGGGAGAAATTTATGTCCTTTCTAAAGAAGAAGGCGGGAGACACACTCCATTCTT
CACCAACTACCGCCCGCAATTCTATGTGCGCACGACTGATGTGACTGGCTCTATCACCCTTCCTGAAGGCGTAGAAATGG
TTATGCCTGGCGATAATGTTAAAATCACCGTAGAGTTGATTAGCCCTGTTGCATTGGAGTTGGGAACCAAATTTGCGATT
CGTGAAGGCGGTAGGACCGTTGGTGCTGGTGTTGTGAGCAATATTATTGAATAA

Upstream 100 bases:

>100_bases
AATGGGGTGAAAAAGTGCTTAAGCTCATGGCTGAAGTGGATGCCTATATCCCTACTCCAGAAAGAGACACTGAAAAAACT
TTCTTGATGCCGGTGAAGAT

Downstream 100 bases:

>100_bases
TATTGGCAAAAAGAGTTACCATAAAGGGTCATTATGAAAGTTAAAATAGGGTTGAAGTGTTCTGATTGTGAAGACATCAA
TTACAGCACAACCAAGAACG

Product: translation elongation factor EF-Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MFSIAGRGTVVTGRIERGVVKVGDEVEIVGIRATQKTTVTGVEMFRKELEKGEAGDNVGVLLRGTKKEEVERGMVLCKPG
SITPHKKFEGEIYVLSKEEGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGDNVKITVELISPVALELGTKFAI
REGGRTVGAGVVSNIIE

Sequences:

>Translated_177_residues
MFSIAGRGTVVTGRIERGVVKVGDEVEIVGIRATQKTTVTGVEMFRKELEKGEAGDNVGVLLRGTKKEEVERGMVLCKPG
SITPHKKFEGEIYVLSKEEGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGDNVKITVELISPVALELGTKFAI
REGGRTVGAGVVSNIIE
>Mature_177_residues
MFSIAGRGTVVTGRIERGVVKVGDEVEIVGIRATQKTTVTGVEMFRKELEKGEAGDNVGVLLRGTKKEEVERGMVLCKPG
SITPHKKFEGEIYVLSKEEGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGDNVKITVELISPVALELGTKFAI
REGGRTVGAGVVSNIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=176, Percent_Identity=50, Blast_Score=197, Evalue=6e-51,
Organism=Escherichia coli, GI1790412, Length=176, Percent_Identity=73.2954545454545, Blast_Score=273, Evalue=4e-75,
Organism=Escherichia coli, GI1789737, Length=176, Percent_Identity=73.2954545454545, Blast_Score=273, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17556456, Length=182, Percent_Identity=39.010989010989, Blast_Score=126, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25141371, Length=175, Percent_Identity=38.2857142857143, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324761, Length=182, Percent_Identity=58.7912087912088, Blast_Score=215, Evalue=2e-57,
Organism=Drosophila melanogaster, GI281363316, Length=175, Percent_Identity=49.7142857142857, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17864358, Length=175, Percent_Identity=49.7142857142857, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI19921738, Length=173, Percent_Identity=39.3063583815029, Blast_Score=140, Evalue=4e-34,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 19212; Mature: 19212

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSIAGRGTVVTGRIERGVVKVGDEVEIVGIRATQKTTVTGVEMFRKELEKGEAGDNVGV
CEEECCCCEEEEEEECCCEEEECCEEEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCEEE
LLRGTKKEEVERGMVLCKPGSITPHKKFEGEIYVLSKEEGGRHTPFFTNYRPQFYVRTTD
EEECCCHHHHHCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEEEC
VTGSITLPEGVEMVMPGDNVKITVELISPVALELGTKFAIREGGRTVGAGVVSNIIE
CEEEEECCCCCEEEECCCCEEEEEEEECCHHHHHCCEEEEECCCCEEHHHHHHHHCC
>Mature Secondary Structure
MFSIAGRGTVVTGRIERGVVKVGDEVEIVGIRATQKTTVTGVEMFRKELEKGEAGDNVGV
CEEECCCCEEEEEEECCCEEEECCEEEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCEEE
LLRGTKKEEVERGMVLCKPGSITPHKKFEGEIYVLSKEEGGRHTPFFTNYRPQFYVRTTD
EEECCCHHHHHCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEEEC
VTGSITLPEGVEMVMPGDNVKITVELISPVALELGTKFAIREGGRTVGAGVVSNIIE
CEEEEECCCCCEEEECCCCEEEEEEEECCHHHHHCCEEEEECCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA