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The map label for this gene is dnaN [H]

Identifier: 108562901

GI number: 108562901

Start: 492262

End: 493386

Strand: Direct

Name: dnaN [H]

Synonym: HPAG1_0476

Alternate gene names: 108562901

Gene position: 492262-493386 (Clockwise)

Preceding gene: 108562900

Following gene: 108562902

Centisome position: 30.84

GC content: 35.91

Gene sequence:

>1125_bases
ATGAAAATCAGTGTTAGTAAAAACGATTTAGAGAATGCTTTGCGCTACTTGCAAGCTTTTTTGGATAAAAAGGACGCTTC
TTCTATCGCTTCACACATCCATTTAGAAGTCATTAAAGAAAAGCTTTTTTTAAAAGCCAGCGATTCAGATATTGGGCTAA
AAAGCTATATTTTTACGCAATCTAGCGATAAAGAGGGCGTAGGCACGATCAACGGGAAAAAGTTTTTAGATATTATCTCA
TGTTTAAAAGACTCTAACATTGTTTTAGAGACTAAAGATGACAGCTTGGTGATCAAACAAAATAAAAGCTCTTTCAAACT
CCCCATGTTTGACGCTGATGAGTTCCCTGAATTCCCTGTTATTGAGCCAAAAGTGAGTATAGAAGTCAATACTCCTTTTT
TGGTAGATGCGTTTAAAAAGATCGCTCCCGTGATTGAGCAAACCAGCCATAAAAGGGAATTAGCCGGTATTTTAATGCAA
TTTGATCAAAAACATCAAACCCTTTCAGTAGTAGGCACGGATACCAAACGGCTCTCTTACACGCAGTTAGAAAAAATCTC
TATCCATTCCACTGAAGAAGATATTTCTTGCATTTTACCTAAAAGAGCTTTATTAGAAATCCTTAAGCTTTTTTATGAAA
ATTTCAGTTTTAAAAGCGATGGTATGTTAGCGGTAATTGAAAATGAAACGCACACTTTTTTCACCAAGCTCATTGATGGG
AATTACCCCGATTATCAAAAAATCCTTCCCAAAGAATACACTTCTTCTTTCACTTTAGGCAAGGAAGAATTTAAAGAGAG
CATTAAGTTATGCAGTTCTTTAAGCTCCACCATTAAACTCACTTTAGAAAAAAACAACGCTTTGTTTGAATCTTTGGATT
CTGAGCATAGCGAAACGGCTAAAACCTCTGTTGAGATTGAAAAAGGTTTGGATATTGAAAAAGCCTTTCATTTGGGCGTG
AACGCTAAATTTTTCCTTGAAGCCTTAAACGCTTTAGGGACAACGCAATTCGTTTTAAAATGCAATGAGCCTTCTTCGCC
TTTTTTGATCCAAGAGTCTCTTGATGAAAAGCAAAGCCACTTGAGCGCTAAAATTTCCACTTTGATGATGCCAATCACAT
TATAA

Upstream 100 bases:

>100_bases
TTTTTTCCAATCGTATAGGGGTAGGAATACGCTTAAACCCTTAAAAAAGCGTTCTTTTATGCTATAATTAAGACCAAAAA
TTCAAGGGGATTATTCAGCT

Downstream 100 bases:

>100_bases
AGGCTTGATTGAATGCAAAATTACCAGAGCCATAGTATTAAGGTTTTAAAAGGCTTAGAGGGGGTTAGGAAACGCCCTGG
AATGTATATTGGCGATACCA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKISVSKNDLENALRYLQAFLDKKDASSIASHIHLEVIKEKLFLKASDSDIGLKSYIFTQSSDKEGVGTINGKKFLDIIS
CLKDSNIVLETKDDSLVIKQNKSSFKLPMFDADEFPEFPVIEPKVSIEVNTPFLVDAFKKIAPVIEQTSHKRELAGILMQ
FDQKHQTLSVVGTDTKRLSYTQLEKISIHSTEEDISCILPKRALLEILKLFYENFSFKSDGMLAVIENETHTFFTKLIDG
NYPDYQKILPKEYTSSFTLGKEEFKESIKLCSSLSSTIKLTLEKNNALFESLDSEHSETAKTSVEIEKGLDIEKAFHLGV
NAKFFLEALNALGTTQFVLKCNEPSSPFLIQESLDEKQSHLSAKISTLMMPITL

Sequences:

>Translated_374_residues
MKISVSKNDLENALRYLQAFLDKKDASSIASHIHLEVIKEKLFLKASDSDIGLKSYIFTQSSDKEGVGTINGKKFLDIIS
CLKDSNIVLETKDDSLVIKQNKSSFKLPMFDADEFPEFPVIEPKVSIEVNTPFLVDAFKKIAPVIEQTSHKRELAGILMQ
FDQKHQTLSVVGTDTKRLSYTQLEKISIHSTEEDISCILPKRALLEILKLFYENFSFKSDGMLAVIENETHTFFTKLIDG
NYPDYQKILPKEYTSSFTLGKEEFKESIKLCSSLSSTIKLTLEKNNALFESLDSEHSETAKTSVEIEKGLDIEKAFHLGV
NAKFFLEALNALGTTQFVLKCNEPSSPFLIQESLDEKQSHLSAKISTLMMPITL
>Mature_374_residues
MKISVSKNDLENALRYLQAFLDKKDASSIASHIHLEVIKEKLFLKASDSDIGLKSYIFTQSSDKEGVGTINGKKFLDIIS
CLKDSNIVLETKDDSLVIKQNKSSFKLPMFDADEFPEFPVIEPKVSIEVNTPFLVDAFKKIAPVIEQTSHKRELAGILMQ
FDQKHQTLSVVGTDTKRLSYTQLEKISIHSTEEDISCILPKRALLEILKLFYENFSFKSDGMLAVIENETHTFFTKLIDG
NYPDYQKILPKEYTSSFTLGKEEFKESIKLCSSLSSTIKLTLEKNNALFESLDSEHSETAKTSVEIEKGLDIEKAFHLGV
NAKFFLEALNALGTTQFVLKCNEPSSPFLIQESLDEKQSHLSAKISTLMMPITL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=342, Percent_Identity=21.0526315789474, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42146; Mature: 42146

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISVSKNDLENALRYLQAFLDKKDASSIASHIHLEVIKEKLFLKASDSDIGLKSYIFTQ
CEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEC
SSDKEGVGTINGKKFLDIISCLKDSNIVLETKDDSLVIKQNKSSFKLPMFDADEFPEFPV
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCC
IEPKVSIEVNTPFLVDAFKKIAPVIEQTSHKRELAGILMQFDQKHQTLSVVGTDTKRLSY
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHCCH
TQLEKISIHSTEEDISCILPKRALLEILKLFYENFSFKSDGMLAVIENETHTFFTKLIDG
HHHHEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCC
NYPDYQKILPKEYTSSFTLGKEEFKESIKLCSSLSSTIKLTLEKNNALFESLDSEHSETA
CCCCHHHHCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCHHHHHH
KTSVEIEKGLDIEKAFHLGVNAKFFLEALNALGTTQFVLKCNEPSSPFLIQESLDEKQSH
HHHEEHHHCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEHHHHHHHHHH
LSAKISTLMMPITL
HHHHHHHHEEEECC
>Mature Secondary Structure
MKISVSKNDLENALRYLQAFLDKKDASSIASHIHLEVIKEKLFLKASDSDIGLKSYIFTQ
CEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEC
SSDKEGVGTINGKKFLDIISCLKDSNIVLETKDDSLVIKQNKSSFKLPMFDADEFPEFPV
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCC
IEPKVSIEVNTPFLVDAFKKIAPVIEQTSHKRELAGILMQFDQKHQTLSVVGTDTKRLSY
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHCCH
TQLEKISIHSTEEDISCILPKRALLEILKLFYENFSFKSDGMLAVIENETHTFFTKLIDG
HHHHEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCC
NYPDYQKILPKEYTSSFTLGKEEFKESIKLCSSLSSTIKLTLEKNNALFESLDSEHSETA
CCCCHHHHCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCHHHHHH
KTSVEIEKGLDIEKAFHLGVNAKFFLEALNALGTTQFVLKCNEPSSPFLIQESLDEKQSH
HHHEEHHHCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEHHHHHHHHHH
LSAKISTLMMPITL
HHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]