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The map label for this gene is bsp6IM [H]

Identifier: 108562885

GI number: 108562885

Start: 473435

End: 473917

Strand: Direct

Name: bsp6IM [H]

Synonym: HPAG1_0460

Alternate gene names: 108562885

Gene position: 473435-473917 (Clockwise)

Preceding gene: 108562884

Following gene: 108562887

Centisome position: 29.66

GC content: 36.44

Gene sequence:

>483_bases
TTGTATAAGGTAGCAGATATTTTTTGTGGTGCTGGAGGATTGAGCTATGGCTTTTCTACACACCCTTATTTTGAATTGAT
ATGGGCTAACGATATAGACAAGGACGCCATTTTAAGCTATCAAGCCAATCATAAAGAGGCACAAACCATTTTATGCGATA
TTATGCAACTTCATTGCCACAACTTGCCATGCGTTCCAATTGATATTCTACTAGGCGGACCACCATGCCAAAGCTATTCT
ACCCTTGGCAAAAGAAAAATGGATGAAAAAGCGAATCTATTTAAAGAATATTTGCGGCTTTTAGATTTAGTGAAACCAAA
AATATTTGTTTTTGAAAATGTGGTGGGTTTAATGTCTATGCAAAAAGGGCAATTGTTCCAACAAATTTGTAACGCTTTTA
AAGAGAGAGGTTATATTTTAGAGCATGCCATTTTGAACGCCCTAGATTATGGTGTGCCTCAAATAAGAGAGGAAGTGTTT
TAG

Upstream 100 bases:

>100_bases
AAAGATTGAAGAAATAGTCTTAACCCAATCTTAAAAAAAGAACTTTAAGTTTTTACTCCATTTAAAAAGTGTGGGTCTAG
ATGAAAGGAAAAAATAAAAC

Downstream 100 bases:

>100_bases
TGGGTGCGCTTAAAAGCTTTAAGCAAAAATCCCACTCCCCCCTTATATTTTGATTGGCCTTAGCCTTTTTATTGCCTCCT
GCTAAAAGTGATCCCATAAA

Product: type II DNA modification enzyme

Products: NA

Alternate protein names: M.Bsp6I; Cytosine-specific methyltransferase Bsp6I [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MYKVADIFCGAGGLSYGFSTHPYFELIWANDIDKDAILSYQANHKEAQTILCDIMQLHCHNLPCVPIDILLGGPPCQSYS
TLGKRKMDEKANLFKEYLRLLDLVKPKIFVFENVVGLMSMQKGQLFQQICNAFKERGYILEHAILNALDYGVPQIREEVF

Sequences:

>Translated_160_residues
MYKVADIFCGAGGLSYGFSTHPYFELIWANDIDKDAILSYQANHKEAQTILCDIMQLHCHNLPCVPIDILLGGPPCQSYS
TLGKRKMDEKANLFKEYLRLLDLVKPKIFVFENVVGLMSMQKGQLFQQICNAFKERGYILEHAILNALDYGVPQIREEVF
>Mature_160_residues
MYKVADIFCGAGGLSYGFSTHPYFELIWANDIDKDAILSYQANHKEAQTILCDIMQLHCHNLPCVPIDILLGGPPCQSYS
TLGKRKMDEKANLFKEYLRLLDLVKPKIFVFENVVGLMSMQKGQLFQQICNAFKERGYILEHAILNALDYGVPQIREEVF

Specific function: This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=171, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=2e-11,
Organism=Homo sapiens, GI4503351, Length=171, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1788271, Length=194, Percent_Identity=27.8350515463918, Blast_Score=66, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 18237; Mature: 18237

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKVADIFCGAGGLSYGFSTHPYFELIWANDIDKDAILSYQANHKEAQTILCDIMQLHCH
CCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHC
NLPCVPIDILLGGPPCQSYSTLGKRKMDEKANLFKEYLRLLDLVKPKIFVFENVVGLMSM
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QKGQLFQQICNAFKERGYILEHAILNALDYGVPQIREEVF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MYKVADIFCGAGGLSYGFSTHPYFELIWANDIDKDAILSYQANHKEAQTILCDIMQLHCH
CCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHC
NLPCVPIDILLGGPPCQSYSTLGKRKMDEKANLFKEYLRLLDLVKPKIFVFENVVGLMSM
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QKGQLFQQICNAFKERGYILEHAILNALDYGVPQIREEVF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607501 [H]