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The map label for this gene is pyrB [H]

Identifier: 108562788

GI number: 108562788

Start: 375276

End: 376199

Strand: Direct

Name: pyrB [H]

Synonym: HPAG1_0363

Alternate gene names: 108562788

Gene position: 375276-376199 (Clockwise)

Preceding gene: 108562779

Following gene: 108562789

Centisome position: 23.51

GC content: 42.1

Gene sequence:

>924_bases
ATGCCAAAAAAATGCCGACACTTGCTCCAAACCAGCGATTTAAGCCTAGATGAAATCAAGCTTTTATTAAAAAAAGCGAG
CGTTTATGCGAACGATTTTAACGCCGTATCTTTAGAAACGAAAGAAAAAATGCACAATAAAATCATCGTGGCTTTATTTT
TTGAAAATTCCACCAGAACGGTGTCTAGTTTTGAAATCGCAAGCCTAAGGTTGGGGGCAAAAATAGTGAAATTAAACATG
CAAACAAGCTCCGCTTCAAAGGGTGAAACTCTAACAGACACTTTTAAAAATATCCATGCCATGCAGCCTGACGCTATCAT
CACACGGCATGCTTTTTCAAGCGCGCCTTTTAAATTAGCTGAATTTTCACAATGCCCCTTGATTAACGCAGGAAGCGGCG
TGAGCGCTCACCCTACCCAAGCGTTATTAGACTTGCTCACCCTTTATCAGCATTTTGGCAGTTTAGAAAATCTAAAAGGG
AAGAAAATCGCTTTCATAGGCGATGTGAAAAATTCCAGAGTGGCTAATAGCAACATTAAATTGCTCCAAAGACTAGGGCT
TGAGATCATGCTGTGCGCTCCAAGCTCCATGCTCCCTACCACTTCTTTAAAAACGACGCACAACATTGAAGAAGCGATAG
CATTTGCAGACATTTTAATGAGTTTGAGGACCCAAACCGAACGGCACAACGCGCCCATTTTTGCGAGCTTGAAAGATTAT
GGCAACGCTTATTGCATCACCCAACAACGCCTAAAGGCTCATGCTAAAAATAAAGAGGTCATTATTTTACACCCAGGCCC
GGTGCATAGGGATATTGATATAGAAAGCGCGGTGTTAGAAGATGAGCGTTCTAAAGTCTTAGAGCAAGTCAAAAATGGCG
TGGCGATGCGCATGGCGGTGTTAGAGTTTTTGCTATTAGACTGA

Upstream 100 bases:

>100_bases
ACACCAGAAGTGTTGAGAGATTTTTCAAGCCCATTCCTTGCTAAAACCCATGCCATTTTTAGCTAAAATAAAACCGATAT
TATAACTAAAAGGGGGTTTT

Downstream 100 bases:

>100_bases
TCATCAAACTTAAAAATGCTTTTTGGCGTTTTTTACAATCAACACAAGAACTTTCATAACTAAAAAAATGGGTTTGGTTG
TAAAACGGCACAAAAATCCC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPKKCRHLLQTSDLSLDEIKLLLKKASVYANDFNAVSLETKEKMHNKIIVALFFENSTRTVSSFEIASLRLGAKIVKLNM
QTSSASKGETLTDTFKNIHAMQPDAIITRHAFSSAPFKLAEFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKG
KKIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPTTSLKTTHNIEEAIAFADILMSLRTQTERHNAPIFASLKDY
GNAYCITQQRLKAHAKNKEVIILHPGPVHRDIDIESAVLEDERSKVLEQVKNGVAMRMAVLEFLLLD

Sequences:

>Translated_307_residues
MPKKCRHLLQTSDLSLDEIKLLLKKASVYANDFNAVSLETKEKMHNKIIVALFFENSTRTVSSFEIASLRLGAKIVKLNM
QTSSASKGETLTDTFKNIHAMQPDAIITRHAFSSAPFKLAEFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKG
KKIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPTTSLKTTHNIEEAIAFADILMSLRTQTERHNAPIFASLKDY
GNAYCITQQRLKAHAKNKEVIILHPGPVHRDIDIESAVLEDERSKVLEQVKNGVAMRMAVLEFLLLD
>Mature_306_residues
PKKCRHLLQTSDLSLDEIKLLLKKASVYANDFNAVSLETKEKMHNKIIVALFFENSTRTVSSFEIASLRLGAKIVKLNMQ
TSSASKGETLTDTFKNIHAMQPDAIITRHAFSSAPFKLAEFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKGK
KIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPTTSLKTTHNIEEAIAFADILMSLRTQTERHNAPIFASLKDYG
NAYCITQQRLKAHAKNKEVIILHPGPVHRDIDIESAVLEDERSKVLEQVKNGVAMRMAVLEFLLLD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=276, Percent_Identity=34.0579710144928, Blast_Score=141, Evalue=7e-34,
Organism=Escherichia coli, GI2367364, Length=323, Percent_Identity=34.0557275541796, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1790703, Length=330, Percent_Identity=25.7575757575758, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786469, Length=204, Percent_Identity=29.9019607843137, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI48994908, Length=250, Percent_Identity=29.6, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=288, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322331, Length=278, Percent_Identity=36.3309352517986, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6322373, Length=329, Percent_Identity=25.2279635258359, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24642586, Length=283, Percent_Identity=33.2155477031802, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34067; Mature: 33936

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKCRHLLQTSDLSLDEIKLLLKKASVYANDFNAVSLETKEKMHNKIIVALFFENSTRT
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCEEEEEEEECCCCCE
VSSFEIASLRLGAKIVKLNMQTSSASKGETLTDTFKNIHAMQPDAIITRHAFSSAPFKLA
EHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
EFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKGKKIAFIGDVKNSRVANSNIK
HHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEECCCCCCCCCCHHH
LLQRLGLEIMLCAPSSMLPTTSLKTTHNIEEAIAFADILMSLRTQTERHNAPIFASLKDY
HHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHC
GNAYCITQQRLKAHAKNKEVIILHPGPVHRDIDIESAVLEDERSKVLEQVKNGVAMRMAV
CCEEEEEHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LEFLLLD
HHHHHCC
>Mature Secondary Structure 
PKKCRHLLQTSDLSLDEIKLLLKKASVYANDFNAVSLETKEKMHNKIIVALFFENSTRT
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCEEEEEEEECCCCCE
VSSFEIASLRLGAKIVKLNMQTSSASKGETLTDTFKNIHAMQPDAIITRHAFSSAPFKLA
EHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
EFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKGKKIAFIGDVKNSRVANSNIK
HHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEECCCCCCCCCCHHH
LLQRLGLEIMLCAPSSMLPTTSLKTTHNIEEAIAFADILMSLRTQTERHNAPIFASLKDY
HHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHC
GNAYCITQQRLKAHAKNKEVIILHPGPVHRDIDIESAVLEDERSKVLEQVKNGVAMRMAV
CCEEEEEHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LEFLLLD
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA