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The map label for this gene is ptsN [H]

Identifier: 103488313

GI number: 103488313

Start: 2990144

End: 2990608

Strand: Reverse

Name: ptsN [H]

Synonym: Sala_2836

Alternate gene names: 103488313

Gene position: 2990608-2990144 (Counterclockwise)

Preceding gene: 103488314

Following gene: 103488312

Centisome position: 89.4

GC content: 66.45

Gene sequence:

>465_bases
ATGAATCTTTCTTCTCTTCTCTATCCGGCGACGGTGCGCGCGCACGTCCATATCGATTCGAAAAAGGCGCTGTTTCCCTT
CGTCGGCGATCTGGCCAGTCGCTCGCTGGGTCTCGACGCCGGCGAAGTGAGCGAAGCCTTGCTCGAACGCGAACGGCTCG
GCTCGACAGGGTTCGGTCGCGGCATCGCGCTGCCCCACGCCAAGATGGCCGACCTCGGCGGCGTGCGCGGCCTGTTCCTC
CAGCTCGCGCGGCCGATCGACTTCAATGCTGTCGATGGCCTGCCGGTCGACCTGCTCTTTGTTCTGCTCTCGCCGCTCGA
TGCCGGTGCCGACCATCTGAAGGCGCTGGCCGGCGTGTCGCGGATGCTGCGCAACGAAGCGATCGCCGAACGGCTGCGCG
GCGCGAAGAACGACGAGGCGCTCTACGCGCTCCTCGCCGACAGCGAAGCGCGTGACGCGGCCTGA

Upstream 100 bases:

>100_bases
CGAAGCCCGGATGGCGGCGGGCCTGCCACGACAGATCCGCGATCGCTTGCCAGCCCTTGTTTCTGGCGGTGTCCGGCGTT
TTACAAGATTTGATCCGACG

Downstream 100 bases:

>100_bases
CAATAAATCAGCCGGGCCCCTGCGACGGCAGGGGCGCCTCATCTGCCGTCACGGTTCAAGTTTATCCCACTTCAGGTCGA
CAGCGTCGGTGATCGGCCCC

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; PTS system EIIA component; Phosphotransferase enzyme IIA component [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MNLSSLLYPATVRAHVHIDSKKALFPFVGDLASRSLGLDAGEVSEALLERERLGSTGFGRGIALPHAKMADLGGVRGLFL
QLARPIDFNAVDGLPVDLLFVLLSPLDAGADHLKALAGVSRMLRNEAIAERLRGAKNDEALYALLADSEARDAA

Sequences:

>Translated_154_residues
MNLSSLLYPATVRAHVHIDSKKALFPFVGDLASRSLGLDAGEVSEALLERERLGSTGFGRGIALPHAKMADLGGVRGLFL
QLARPIDFNAVDGLPVDLLFVLLSPLDAGADHLKALAGVSRMLRNEAIAERLRGAKNDEALYALLADSEARDAA
>Mature_154_residues
MNLSSLLYPATVRAHVHIDSKKALFPFVGDLASRSLGLDAGEVSEALLERERLGSTGFGRGIALPHAKMADLGGVRGLFL
QLARPIDFNAVDGLPVDLLFVLLSPLDAGADHLKALAGVSRMLRNEAIAERLRGAKNDEALYALLADSEARDAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=150, Percent_Identity=44.6666666666667, Blast_Score=131, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16357; Mature: 16357

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSSLLYPATVRAHVHIDSKKALFPFVGDLASRSLGLDAGEVSEALLERERLGSTGFGR
CCCCHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIALPHAKMADLGGVRGLFLQLARPIDFNAVDGLPVDLLFVLLSPLDAGADHLKALAGVS
CCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RMLRNEAIAERLRGAKNDEALYALLADSEARDAA
HHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCC
>Mature Secondary Structure
MNLSSLLYPATVRAHVHIDSKKALFPFVGDLASRSLGLDAGEVSEALLERERLGSTGFGR
CCCCHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIALPHAKMADLGGVRGLFLQLARPIDFNAVDGLPVDLLFVLLSPLDAGADHLKALAGVS
CCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RMLRNEAIAERLRGAKNDEALYALLADSEARDAA
HHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7876255; 8444818; 8025669; 9278503; 9636714 [H]