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The map label for this gene is iaaA [H]

Identifier: 103488301

GI number: 103488301

Start: 2978359

End: 2979420

Strand: Reverse

Name: iaaA [H]

Synonym: Sala_2824

Alternate gene names: 103488301

Gene position: 2979420-2978359 (Counterclockwise)

Preceding gene: 103488302

Following gene: 103488300

Centisome position: 89.07

GC content: 71.75

Gene sequence:

>1062_bases
ATGATCCTTTCGCTCGCCGCCGCCGCGCTCGCCGCCGCACAGGCCGCCGCGCCCCAGCCCTTTGCCGACGCCGCGGCCCC
GCCAGCGGCGGAAACGCGCACCGACCGGGCTGAGGTTCCCGCGACGAGCGGCGGCTGGACGCTCGTCGTTCATGGCGGCG
CGGGCACGATCCGCCGCGACCGCATCACGGCGGAGCAGGACGCCGCCTATCGCGCGGCAATCGATGCGGCGCTGATCGCG
GGGCAGGCGGTGCTGGCAAAGGGCGGCAGCGCGCTCGACGCGGTGCAGGCGGCGGTCGAGCTGATGGAGGATGATCCGCT
GTTCAACGCGGGGCGCGGCGCGGTCTTCACCCATGACCGCACCAACGAACTCGACGCCGCGATCATGGACGGTCGCACGC
GCGCGGCGGGTGCGGTCGCGCGGCTGACGACCAGCCGCCACCCGGTCGCGGCGGCGCGTGCGGTGCTGCGCGACGGGCGG
CACGTCATGCTGACCGGCACCGACGCCGACCGCTTCGCCGCCGAACGCGGGGTCGAGCAGATGGACCCCGCCTGGTTCGC
GACATCGCATCGCCGCGAGCAGATCGACGAGTTTCTGCGACGCGAGGCGGGCGCAAAGACCGTGTCGTCCTATGATATCG
ACCAGAAGTTCGGGACCGTCGGAGCGGTGGCGCGCGACACGCACGGCAATCTGGCTGCGGCGACCTCGACGGGCGGACTT
ACGGGCAAAAGATGGGGGCGGATCGGCGACGCGCCGATCGTCGGCGCGGGCACCTATGCCGACGACCGCGCCTGCGCGGT
GTCGGCGACGGGGGCAGGTGAGTATTTCATCCGCGTCGGCGTCGCGCACGAAATTTGCGCGCGCGTGCGGCTGGCCGGCC
AATCGCTGCAGGAGGCGGCCGACGCGGTGATGGCCGAGGTGCTGGCGCTCGGCGGATCGGGTGGGGTGATCGTCGCGGGC
CCCGACGGCGGGTCGGCGTTCAGCTTCAACACCGAAGGCATGTATCGTGGCCGCATGACGAGCGCGGGTGTGCATGAGGT
GGCGATTTATAACGATGAATAG

Upstream 100 bases:

>100_bases
GTCCGCGTGCTCAAAAAGGTCGATTTCGTCATGGCGCGGGGCAGGGTGGTCGACTGAACCGCCACCCTTTGCGTCTCCCG
CTTGCTTCAAGGAATGAACG

Downstream 100 bases:

>100_bases
GGTACCGAGCGCAGACCCCATTGACCGGTGGGGCATTCGGAGCTAGAGGGCCGCCAGTTCGGGAATGGCGCTTTGTCGCG
GTTTTCGAGTCACAACAGCG

Product: peptidase T2, asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILSLAAAALAAAQAAAPQPFADAAAPPAAETRTDRAEVPATSGGWTLVVHGGAGTIRRDRITAEQDAAYRAAIDAALIA
GQAVLAKGGSALDAVQAAVELMEDDPLFNAGRGAVFTHDRTNELDAAIMDGRTRAAGAVARLTTSRHPVAAARAVLRDGR
HVMLTGTDADRFAAERGVEQMDPAWFATSHRREQIDEFLRREAGAKTVSSYDIDQKFGTVGAVARDTHGNLAAATSTGGL
TGKRWGRIGDAPIVGAGTYADDRACAVSATGAGEYFIRVGVAHEICARVRLAGQSLQEAADAVMAEVLALGGSGGVIVAG
PDGGSAFSFNTEGMYRGRMTSAGVHEVAIYNDE

Sequences:

>Translated_353_residues
MILSLAAAALAAAQAAAPQPFADAAAPPAAETRTDRAEVPATSGGWTLVVHGGAGTIRRDRITAEQDAAYRAAIDAALIA
GQAVLAKGGSALDAVQAAVELMEDDPLFNAGRGAVFTHDRTNELDAAIMDGRTRAAGAVARLTTSRHPVAAARAVLRDGR
HVMLTGTDADRFAAERGVEQMDPAWFATSHRREQIDEFLRREAGAKTVSSYDIDQKFGTVGAVARDTHGNLAAATSTGGL
TGKRWGRIGDAPIVGAGTYADDRACAVSATGAGEYFIRVGVAHEICARVRLAGQSLQEAADAVMAEVLALGGSGGVIVAG
PDGGSAFSFNTEGMYRGRMTSAGVHEVAIYNDE
>Mature_353_residues
MILSLAAAALAAAQAAAPQPFADAAAPPAAETRTDRAEVPATSGGWTLVVHGGAGTIRRDRITAEQDAAYRAAIDAALIA
GQAVLAKGGSALDAVQAAVELMEDDPLFNAGRGAVFTHDRTNELDAAIMDGRTRAAGAVARLTTSRHPVAAARAVLRDGR
HVMLTGTDADRFAAERGVEQMDPAWFATSHRREQIDEFLRREAGAKTVSSYDIDQKFGTVGAVARDTHGNLAAATSTGGL
TGKRWGRIGDAPIVGAGTYADDRACAVSATGAGEYFIRVGVAHEICARVRLAGQSLQEAADAVMAEVLALGGSGGVIVAG
PDGGSAFSFNTEGMYRGRMTSAGVHEVAIYNDE

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=278, Percent_Identity=39.9280575539568, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI145275200, Length=278, Percent_Identity=39.9280575539568, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI98991769, Length=354, Percent_Identity=31.0734463276836, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1787050, Length=298, Percent_Identity=51.006711409396, Blast_Score=267, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI32564649, Length=216, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18921183, Length=304, Percent_Identity=40.1315789473684, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21357893, Length=266, Percent_Identity=31.9548872180451, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 36358; Mature: 36358

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSLAAAALAAAQAAAPQPFADAAAPPAAETRTDRAEVPATSGGWTLVVHGGAGTIRRD
CCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECC
RITAEQDAAYRAAIDAALIAGQAVLAKGGSALDAVQAAVELMEDDPLFNAGRGAVFTHDR
CCCCHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
TNELDAAIMDGRTRAAGAVARLTTSRHPVAAARAVLRDGRHVMLTGTDADRFAAERGVEQ
CCCHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCHHH
MDPAWFATSHRREQIDEFLRREAGAKTVSSYDIDQKFGTVGAVARDTHGNLAAATSTGGL
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHCCHHHEEECCCCCEEEECCCCCC
TGKRWGRIGDAPIVGAGTYADDRACAVSATGAGEYFIRVGVAHEICARVRLAGQSLQEAA
CCHHCCCCCCCCEEECCCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
DAVMAEVLALGGSGGVIVAGPDGGSAFSFNTEGMYRGRMTSAGVHEVAIYNDE
HHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCEECCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MILSLAAAALAAAQAAAPQPFADAAAPPAAETRTDRAEVPATSGGWTLVVHGGAGTIRRD
CCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECC
RITAEQDAAYRAAIDAALIAGQAVLAKGGSALDAVQAAVELMEDDPLFNAGRGAVFTHDR
CCCCHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
TNELDAAIMDGRTRAAGAVARLTTSRHPVAAARAVLRDGRHVMLTGTDADRFAAERGVEQ
CCCHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCHHH
MDPAWFATSHRREQIDEFLRREAGAKTVSSYDIDQKFGTVGAVARDTHGNLAAATSTGGL
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHCCHHHEEECCCCCEEEECCCCCC
TGKRWGRIGDAPIVGAGTYADDRACAVSATGAGEYFIRVGVAHEICARVRLAGQSLQEAA
CCHHCCCCCCCCEEECCCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
DAVMAEVLALGGSGGVIVAGPDGGSAFSFNTEGMYRGRMTSAGVHEVAIYNDE
HHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCEECCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]