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The map label for this gene is priA [H]

Identifier: 103487760

GI number: 103487760

Start: 2400773

End: 2402941

Strand: Direct

Name: priA [H]

Synonym: Sala_2279

Alternate gene names: 103487760

Gene position: 2400773-2402941 (Clockwise)

Preceding gene: 103487759

Following gene: 103487761

Centisome position: 71.77

GC content: 71.32

Gene sequence:

>2169_bases
ATGACCCGCGCCCGCGTCCTCTTGCTCACCGCCGCACTCGGCCCGCTCGACTATCGCGTGCCGCAGGGCAGCGAAGCGCC
CCTCGGCAGCGTCGTCATCGCGCCGCTCGGCCCGCGACGGATTGGCGGCGTGGTGTGGGAGGATGAAAGCTTCGGCGCGC
CCGACCCCGTCGGCGACAACCGGCTTCGCAACCTTTACGAAATCGTCCCCGTCCCGCCGATCGCCGCGCCGCTCCGCCGC
CTCGTCGAATGGACGAGCGACTATTATCTAAGCCCCCCCGGCTCGGTGCTGCGCATGTTGTTGCCCGGCGTCGCCTTCGC
CGATGCGCGGCGCCCGATCGTCGAATATCGCGCGACGGGCGAACTGCCCGCGCGGATGACGCCGCAGCGGACAGTCGCGC
TGGGGAAGATCGGCGCGCGCCAGGGGACGGTGCGCGAGCTGGCGAAGCTCGCCGACGTCAGCGAGGCGGTGATCCGCGGC
CTCGTCCATGTCGGCGCACTCGAAGCGGTGAGCGCTTCGGCCGACCAGCCCTGTCCCGAACCCGATCCCGCCTTTGCGCC
GCCGGACCTGTCGGCGGAACAGACGGCGGCGGCGGCGCAGTTGCGCGCAGCGGTTGCCGCCGCGAGGTTCGAAACATTGC
TGCTCGACGGCGTCACCGGATCGGGCAAGACCGAAGTTTATATGGAGGCGATCGCTGCCGCGGTGGACGCGGGGCGGCAG
GCGCTGGTGCTGCTCCCCGAAATCGCGCTCACCGAACCGATGTTGACGCGCTTTGCCGCGCGTTTCGGTTGTGAACCGGT
CGCGTGGCATTCGGGCCTGCGCTCCACCGAGCGCCGCCGCGCCTGGCATGCGATTGCCAGCGGCGAGGCACGGATCGTCG
TCGGGGCGCGCTCGGCGCTGTTCCTGCCCTATGCGCGGCTCGGCGTCATCGTCGTCGACGAGGCGCATGAGACGAGCTTC
AAGCAGGAGGATGGCGTCCATTATCACGCGCGCGACGTCGCGGTGATGCGCGGGTATTTCGAGGGCCTGCCGGTGATCCT
CGCGAGCGCCACCCCGGCGCTCGAAAGCCTCGCACAGGTCGAGGCCGGGCGCTATCGCCATATCCAGCTGCCCGCGCGTT
TCGGCGGCGCCACGCTGCCCGATCTCGCCGCGATCGACATGCGGCAGGATCCACCCGACCGCGGCCGCTGGCTGGCGCCG
CCGCTCGTCGACGCACTCGCCGACCGGCTCGAAAAGGGCGAGCAGAGCCTGCTGTTCCTCAATCGCCGCGGCTTTGCGCC
GCTGACGCTCTGCCGTACCTGCGGCCACCGCATCCAGTGCCCCAATTGCACCGCGTGGATGGTCGAGCACCGGCTCGTCC
ACCGCCTCGCCTGCCATCATTGCGGCCATGTCATGCCGCCGCCGCGCCTCTGCCCCGAATGCGAGGACGAGGACAGCCTC
GTCGCCTGTGGCCCCGGCGTCGAGCGCGTCGCCGACGAAGTCGCGCTGCGTTTCCCGGAGGCGCGCACCGCCATCGTCAC
CTCCGACACGCTCTGGTCGCCCGCCAGGGCCGCCGAGTTCGTGGCCAATGTCGAGCAGGGGCTGGTCGACATCATCATCG
GCACCCAGCTCGTCACCAAGGGCTATCATTTCCCGAACCTCACGCTCGTGGGCGTCGTCGATGCCGACCTCGGCCTCGAC
GGCGGCGACCTGCGTGCGTCGGAACGCACCTTCCAGCAGATCGCGCAGGTCGCGGGGCGCGCCGGGCGCGGGGTCAAGCC
CGGCGAGGTGCTGATCCAGACGCGGGTGCCCGACGCGCCGGTGATGGCCGCGCTCGTCGCGGGCGACCGCGACGGCTTCT
ACGCCGCCGAGGCCGCGGCGCGGCGCGCGGCGGGCGCGCCGCCCTATGGCCGCTTCGCAGCGCTGGTCATTTCGTCGGAG
GACATCGAGGTCGCGCGCGGCGTCGCGCAGCGATTGGGGGCAAAGGCGCCCGTCGCCGACGGCCTCGCCGTCTATGGCCC
CGCCCCGGCCCCCCTCGCAATGCTGCGCGGCCGCCACCGCTTCCGCCTGCTCCTCCACGCCCCGCGCAGCTTTGACCTGC
AAGGGACGATCCGCGCGTGGGTGGACAGCATCGACTGGCCCGCAAAGGCGCGGCTCGCGATCGACGTCGATCCCTATAGT
TTCGTTTGA

Upstream 100 bases:

>100_bases
TGCCAAATGCGGTCCTTTCCAGCAGAATCGACCGTTAGCCGCCATCGCCGCCCTCGGTGCTTTCCTATTTTCCCCGCGCG
CCTATATCCTTGCCCGACCC

Downstream 100 bases:

>100_bases
ACGCCGCTATTTACATCGCTCCGGAGCGGCGTCAAAAGCTTGGTGGGGACCATCAGGGCCGGGGGAACCGAAGATGCGGA
AAGCCACGATCGCCGTCATG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MTRARVLLLTAALGPLDYRVPQGSEAPLGSVVIAPLGPRRIGGVVWEDESFGAPDPVGDNRLRNLYEIVPVPPIAAPLRR
LVEWTSDYYLSPPGSVLRMLLPGVAFADARRPIVEYRATGELPARMTPQRTVALGKIGARQGTVRELAKLADVSEAVIRG
LVHVGALEAVSASADQPCPEPDPAFAPPDLSAEQTAAAAQLRAAVAAARFETLLLDGVTGSGKTEVYMEAIAAAVDAGRQ
ALVLLPEIALTEPMLTRFAARFGCEPVAWHSGLRSTERRRAWHAIASGEARIVVGARSALFLPYARLGVIVVDEAHETSF
KQEDGVHYHARDVAVMRGYFEGLPVILASATPALESLAQVEAGRYRHIQLPARFGGATLPDLAAIDMRQDPPDRGRWLAP
PLVDALADRLEKGEQSLLFLNRRGFAPLTLCRTCGHRIQCPNCTAWMVEHRLVHRLACHHCGHVMPPPRLCPECEDEDSL
VACGPGVERVADEVALRFPEARTAIVTSDTLWSPARAAEFVANVEQGLVDIIIGTQLVTKGYHFPNLTLVGVVDADLGLD
GGDLRASERTFQQIAQVAGRAGRGVKPGEVLIQTRVPDAPVMAALVAGDRDGFYAAEAAARRAAGAPPYGRFAALVISSE
DIEVARGVAQRLGAKAPVADGLAVYGPAPAPLAMLRGRHRFRLLLHAPRSFDLQGTIRAWVDSIDWPAKARLAIDVDPYS
FV

Sequences:

>Translated_722_residues
MTRARVLLLTAALGPLDYRVPQGSEAPLGSVVIAPLGPRRIGGVVWEDESFGAPDPVGDNRLRNLYEIVPVPPIAAPLRR
LVEWTSDYYLSPPGSVLRMLLPGVAFADARRPIVEYRATGELPARMTPQRTVALGKIGARQGTVRELAKLADVSEAVIRG
LVHVGALEAVSASADQPCPEPDPAFAPPDLSAEQTAAAAQLRAAVAAARFETLLLDGVTGSGKTEVYMEAIAAAVDAGRQ
ALVLLPEIALTEPMLTRFAARFGCEPVAWHSGLRSTERRRAWHAIASGEARIVVGARSALFLPYARLGVIVVDEAHETSF
KQEDGVHYHARDVAVMRGYFEGLPVILASATPALESLAQVEAGRYRHIQLPARFGGATLPDLAAIDMRQDPPDRGRWLAP
PLVDALADRLEKGEQSLLFLNRRGFAPLTLCRTCGHRIQCPNCTAWMVEHRLVHRLACHHCGHVMPPPRLCPECEDEDSL
VACGPGVERVADEVALRFPEARTAIVTSDTLWSPARAAEFVANVEQGLVDIIIGTQLVTKGYHFPNLTLVGVVDADLGLD
GGDLRASERTFQQIAQVAGRAGRGVKPGEVLIQTRVPDAPVMAALVAGDRDGFYAAEAAARRAAGAPPYGRFAALVISSE
DIEVARGVAQRLGAKAPVADGLAVYGPAPAPLAMLRGRHRFRLLLHAPRSFDLQGTIRAWVDSIDWPAKARLAIDVDPYS
FV
>Mature_721_residues
TRARVLLLTAALGPLDYRVPQGSEAPLGSVVIAPLGPRRIGGVVWEDESFGAPDPVGDNRLRNLYEIVPVPPIAAPLRRL
VEWTSDYYLSPPGSVLRMLLPGVAFADARRPIVEYRATGELPARMTPQRTVALGKIGARQGTVRELAKLADVSEAVIRGL
VHVGALEAVSASADQPCPEPDPAFAPPDLSAEQTAAAAQLRAAVAAARFETLLLDGVTGSGKTEVYMEAIAAAVDAGRQA
LVLLPEIALTEPMLTRFAARFGCEPVAWHSGLRSTERRRAWHAIASGEARIVVGARSALFLPYARLGVIVVDEAHETSFK
QEDGVHYHARDVAVMRGYFEGLPVILASATPALESLAQVEAGRYRHIQLPARFGGATLPDLAAIDMRQDPPDRGRWLAPP
LVDALADRLEKGEQSLLFLNRRGFAPLTLCRTCGHRIQCPNCTAWMVEHRLVHRLACHHCGHVMPPPRLCPECEDEDSLV
ACGPGVERVADEVALRFPEARTAIVTSDTLWSPARAAEFVANVEQGLVDIIIGTQLVTKGYHFPNLTLVGVVDADLGLDG
GDLRASERTFQQIAQVAGRAGRGVKPGEVLIQTRVPDAPVMAALVAGDRDGFYAAEAAARRAAGAPPYGRFAALVISSED
IEVARGVAQRLGAKAPVADGLAVYGPAPAPLAMLRGRHRFRLLLHAPRSFDLQGTIRAWVDSIDWPAKARLAIDVDPYSF
V

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=727, Percent_Identity=36.5887207702889, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 77541; Mature: 77410

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00690 DEAH_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRARVLLLTAALGPLDYRVPQGSEAPLGSVVIAPLGPRRIGGVVWEDESFGAPDPVGDN
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCEEEECCCCCCCCCCCCH
RLRNLYEIVPVPPIAAPLRRLVEWTSDYYLSPPGSVLRMLLPGVAFADARRPIVEYRATG
HHHHHHHHCCCCCHHHHHHHHHHHHCCCEECCHHHHHHHHHCCCHHHHCCCCCEEEECCC
ELPARMTPQRTVALGKIGARQGTVRELAKLADVSEAVIRGLVHVGALEAVSASADQPCPE
CCCCCCCCCHHEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDPAFAPPDLSAEQTAAAAQLRAAVAAARFETLLLDGVTGSGKTEVYMEAIAAAVDAGRQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCCC
ALVLLPEIALTEPMLTRFAARFGCEPVAWHSGLRSTERRRAWHAIASGEARIVVGARSAL
EEEEECHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCHHH
FLPYARLGVIVVDEAHETSFKQEDGVHYHARDVAVMRGYFEGLPVILASATPALESLAQV
HCCHHHCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
EAGRYRHIQLPARFGGATLPDLAAIDMRQDPPDRGRWLAPPLVDALADRLEKGEQSLLFL
HCCCEEEEECCHHCCCCCCCCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEE
NRRGFAPLTLCRTCGHRIQCPNCTAWMVEHRLVHRLACHHCGHVMPPPRLCPECEDEDSL
ECCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
VACGPGVERVADEVALRFPEARTAIVTSDTLWSPARAAEFVANVEQGLVDIIIGTQLVTK
EEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYHFPNLTLVGVVDADLGLDGGDLRASERTFQQIAQVAGRAGRGVKPGEVLIQTRVPDAP
CCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCH
VMAALVAGDRDGFYAAEAAARRAAGAPPYGRFAALVISSEDIEVARGVAQRLGAKAPVAD
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCC
GLAVYGPAPAPLAMLRGRHRFRLLLHAPRSFDLQGTIRAWVDSIDWPAKARLAIDVDPYS
CEEEECCCCCCHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCC
FV
CC
>Mature Secondary Structure 
TRARVLLLTAALGPLDYRVPQGSEAPLGSVVIAPLGPRRIGGVVWEDESFGAPDPVGDN
CCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCEEEECCCCCCCCCCCCH
RLRNLYEIVPVPPIAAPLRRLVEWTSDYYLSPPGSVLRMLLPGVAFADARRPIVEYRATG
HHHHHHHHCCCCCHHHHHHHHHHHHCCCEECCHHHHHHHHHCCCHHHHCCCCCEEEECCC
ELPARMTPQRTVALGKIGARQGTVRELAKLADVSEAVIRGLVHVGALEAVSASADQPCPE
CCCCCCCCCHHEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDPAFAPPDLSAEQTAAAAQLRAAVAAARFETLLLDGVTGSGKTEVYMEAIAAAVDAGRQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCCC
ALVLLPEIALTEPMLTRFAARFGCEPVAWHSGLRSTERRRAWHAIASGEARIVVGARSAL
EEEEECHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCHHH
FLPYARLGVIVVDEAHETSFKQEDGVHYHARDVAVMRGYFEGLPVILASATPALESLAQV
HCCHHHCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
EAGRYRHIQLPARFGGATLPDLAAIDMRQDPPDRGRWLAPPLVDALADRLEKGEQSLLFL
HCCCEEEEECCHHCCCCCCCCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEE
NRRGFAPLTLCRTCGHRIQCPNCTAWMVEHRLVHRLACHHCGHVMPPPRLCPECEDEDSL
ECCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
VACGPGVERVADEVALRFPEARTAIVTSDTLWSPARAAEFVANVEQGLVDIIIGTQLVTK
EEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYHFPNLTLVGVVDADLGLDGGDLRASERTFQQIAQVAGRAGRGVKPGEVLIQTRVPDAP
CCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCH
VMAALVAGDRDGFYAAEAAARRAAGAPPYGRFAALVISSEDIEVARGVAQRLGAKAPVAD
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCC
GLAVYGPAPAPLAMLRGRHRFRLLLHAPRSFDLQGTIRAWVDSIDWPAKARLAIDVDPYS
CEEEECCCCCCHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCC
FV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]