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The map label for this gene is ptsP [H]

Identifier: 103487630

GI number: 103487630

Start: 2258079

End: 2260376

Strand: Direct

Name: ptsP [H]

Synonym: Sala_2149

Alternate gene names: 103487630

Gene position: 2258079-2260376 (Clockwise)

Preceding gene: 103487629

Following gene: 103487635

Centisome position: 67.5

GC content: 67.15

Gene sequence:

>2298_bases
ATGACCAACGCCCCGCCCCCGCCCTCGACGGCCGCCCAGTCGGCGCGCACCATATTGACCCGGCTGCACGAGGTCATGGC
GTCGCGTGCCAATGCGCAGGGCAAGCTCAATCAGGTCGTCGGGATCATCGGCGAATGCCTCGACAGCGAAGTCTGCTCGA
TCTACCTGCTGCGCGACGGCGCGCTCGAACTTTATGCGACGCGCGGCCTCAAGCAGGAGGCGGTGCATGTCACACGCCTT
GCGCCCGGCGAAGGGCTTGTGGGCACCATTGCCGAACATATCGAAACGCTCAATCTCGACGAAGCCGCAGCGCACCCCGA
CTTTTCCTATCGCCCCGAAACGGGCGAGGAATTGTTCCACAGCTTTGCCGGGGTGCCGATCATCCGTCGCGAGCGCGCCG
TTGGCGTCCTTTGCGTCCAGCACGCGGAACCGCGCCGTTATGAAGAGATCGAGATCGAGACGCTGCAAACGGTCGCGATG
GTGCTGTCCGAACTGATCGCCAACGCCGACCTTGTCGATACCGCCGCACGCACCGACGCCGCGGCCGCCGACCAGTCGGC
GCAGCGGCTGAACGGACAGAAGCTGGTCGACGGCATGGGCGCCGGGGTCGCGGTATTCCATCAGCCGCGCATCACCATCG
AACATACGGTCGCCGACGATGTCGAGGCCGAACGCCACCGCGTCTACGCCGCCTTCGACAAGATGCGCGAACAGATCGAC
CGCATGGCAAGCCAGGCCGAATTCGGCGTCGGCGGCGAGCATGAAGAGGTGCTCGAAACCTACAAGATGTTCGCCTATGA
CGAAGGCTGGTCGCGGCGGATCAACGAAGCGATCGACAGCGGCCTGACCGCAGAGGCGGCGATCGAGCGCGTCCAGCAAC
GCACGCGGATGCGGATGCGCCAGATCGACGATCCGCTGCTCCGCGACCGGATGCACGACCTCGAAGATCTGTCGAACCGG
CTGATCCGCATCGTGTCCGGACAGATGGGCACCGCAGCGCAAATGGGGCTCCGGCAGGATTCGATCCTGATCGCACGCAA
CCTCGGTCCGGCCGAACTGCTCGAATATGATCGCCGCCGCCTGAAAGGCGTCGTGCTCGAAGAAGGCTCGCTGACCGCCC
ATGTCATCATCGTTGCGCGCGCGATGGGCGTCCCGGTCATCGGCCGCGTCCGCGACGTGCGCACCTCGATCCGCGAGGGC
GACCTGCTGCTGCTCGATGCCAGCGCGGGCACTGTGCATGTCCGCCCGACGCCCGCGGTGCAGGAGGCTTTCAACGCCAA
GCTCGCCATCTCGCAAAAGCGCCGCGCCGATCTTGCGGCGCTGCGCGACCTTCCTGCGGTCACCAGGGATGGGGTTCCGA
TCGAGTTGATGATCAACGCCGGCCTGCGCGAGGATGTCGCTGCGCTCGACCTCACCGGCGCGCGCGGGATCGGGCTGTTC
CGCACCGAGTTCCAGTTCCTCGTCTCGGCGACGCTGCCCGCGCGCGAACGGCAGCAGCGGCTGTATCGCGACGTGCTCGA
TGCCGCGGGCGACCGCCCGGTCATCTTCCGCACCGTCGACATCGGCGGCGACAAGGCGCTGCCCTATATGAATGTTGGCG
AAGGCGCGCAGGAGGAAAACCCGGCGATGGGCTGGCGCGCGCTCCGTCTCGCGCTCGACCGCGAAGGTCTTTTGAAGGTG
CAGGCGCGCGCGCTGATGGAAGCCGCGGCCGGGCGTACGCTCAACGTCATGTTCCCGATGGTGTCGGAACCATGGGAATA
TGAGGCGGCGCGCGCCTTGTTCGTCGGCCAGCGTGCCTGGCTCGCCAGCCACAACAAGAAGCTGCCGGTGGCGATCCGCT
ATGGCGCGATGCTCGAGGTGCCCGGGCTGGTCGAAACGCTCGACCTGATGCTGCCGCACCTCGACTTCCTGTCGATCGGC
ACCAACGACCTCACCCAGTTCCTCTTCGCCGCCGACCGGGCGCACCCGCGGCTCGCCGAACGCTACGACTGGCTGTCGCC
GACGGTGATGCGCTATCTGGCGCGCGTGGTGAAGATCGTGTCGGGATCGAAGGTCGCGCTCGGCGTGTGCGGCGAAATGG
GGGGACGCCCCCTGGAAGCCATGGCGCTGCTCGGTCTCGGCATCGAACGCCTGTCGATCACCCCCGCGGGCGTCGGCCCG
GTCAAGGCGATGATCCGCTCGCTCGACCTCGGCGCGCTGCGCGCCGACATGCCCGCGATGCTGGCGCAGCCGGCGCCGGA
CCCGCGCGGGCAGTATGAGCAATGGGCGCTCGCCCATCAGGTCGACCTGGGCACGTAA

Upstream 100 bases:

>100_bases
CCGTCCGGCAGGTAGCGCGAGGTCCAGCGGGCCAGGAATCTATGGGGCGACCGTGCGCGACCTATTGGCGCGCGCCGCAA
CCTTCTGATAGCAGGGAGCG

Downstream 100 bases:

>100_bases
ATGCGCCACAGTCGGCCATAGTTGGGCTATTCCAGTCAGGTCGTCGCTGGCACGGTCGTGATCGCCGGGACCGCCTGCCC
GTCGTTCGCGGTGCGAACGA

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MTNAPPPPSTAAQSARTILTRLHEVMASRANAQGKLNQVVGIIGECLDSEVCSIYLLRDGALELYATRGLKQEAVHVTRL
APGEGLVGTIAEHIETLNLDEAAAHPDFSYRPETGEELFHSFAGVPIIRRERAVGVLCVQHAEPRRYEEIEIETLQTVAM
VLSELIANADLVDTAARTDAAAADQSAQRLNGQKLVDGMGAGVAVFHQPRITIEHTVADDVEAERHRVYAAFDKMREQID
RMASQAEFGVGGEHEEVLETYKMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMRQIDDPLLRDRMHDLEDLSNR
LIRIVSGQMGTAAQMGLRQDSILIARNLGPAELLEYDRRRLKGVVLEEGSLTAHVIIVARAMGVPVIGRVRDVRTSIREG
DLLLLDASAGTVHVRPTPAVQEAFNAKLAISQKRRADLAALRDLPAVTRDGVPIELMINAGLREDVAALDLTGARGIGLF
RTEFQFLVSATLPARERQQRLYRDVLDAAGDRPVIFRTVDIGGDKALPYMNVGEGAQEENPAMGWRALRLALDREGLLKV
QARALMEAAAGRTLNVMFPMVSEPWEYEAARALFVGQRAWLASHNKKLPVAIRYGAMLEVPGLVETLDLMLPHLDFLSIG
TNDLTQFLFAADRAHPRLAERYDWLSPTVMRYLARVVKIVSGSKVALGVCGEMGGRPLEAMALLGLGIERLSITPAGVGP
VKAMIRSLDLGALRADMPAMLAQPAPDPRGQYEQWALAHQVDLGT

Sequences:

>Translated_765_residues
MTNAPPPPSTAAQSARTILTRLHEVMASRANAQGKLNQVVGIIGECLDSEVCSIYLLRDGALELYATRGLKQEAVHVTRL
APGEGLVGTIAEHIETLNLDEAAAHPDFSYRPETGEELFHSFAGVPIIRRERAVGVLCVQHAEPRRYEEIEIETLQTVAM
VLSELIANADLVDTAARTDAAAADQSAQRLNGQKLVDGMGAGVAVFHQPRITIEHTVADDVEAERHRVYAAFDKMREQID
RMASQAEFGVGGEHEEVLETYKMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMRQIDDPLLRDRMHDLEDLSNR
LIRIVSGQMGTAAQMGLRQDSILIARNLGPAELLEYDRRRLKGVVLEEGSLTAHVIIVARAMGVPVIGRVRDVRTSIREG
DLLLLDASAGTVHVRPTPAVQEAFNAKLAISQKRRADLAALRDLPAVTRDGVPIELMINAGLREDVAALDLTGARGIGLF
RTEFQFLVSATLPARERQQRLYRDVLDAAGDRPVIFRTVDIGGDKALPYMNVGEGAQEENPAMGWRALRLALDREGLLKV
QARALMEAAAGRTLNVMFPMVSEPWEYEAARALFVGQRAWLASHNKKLPVAIRYGAMLEVPGLVETLDLMLPHLDFLSIG
TNDLTQFLFAADRAHPRLAERYDWLSPTVMRYLARVVKIVSGSKVALGVCGEMGGRPLEAMALLGLGIERLSITPAGVGP
VKAMIRSLDLGALRADMPAMLAQPAPDPRGQYEQWALAHQVDLGT
>Mature_764_residues
TNAPPPPSTAAQSARTILTRLHEVMASRANAQGKLNQVVGIIGECLDSEVCSIYLLRDGALELYATRGLKQEAVHVTRLA
PGEGLVGTIAEHIETLNLDEAAAHPDFSYRPETGEELFHSFAGVPIIRRERAVGVLCVQHAEPRRYEEIEIETLQTVAMV
LSELIANADLVDTAARTDAAAADQSAQRLNGQKLVDGMGAGVAVFHQPRITIEHTVADDVEAERHRVYAAFDKMREQIDR
MASQAEFGVGGEHEEVLETYKMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMRQIDDPLLRDRMHDLEDLSNRL
IRIVSGQMGTAAQMGLRQDSILIARNLGPAELLEYDRRRLKGVVLEEGSLTAHVIIVARAMGVPVIGRVRDVRTSIREGD
LLLLDASAGTVHVRPTPAVQEAFNAKLAISQKRRADLAALRDLPAVTRDGVPIELMINAGLREDVAALDLTGARGIGLFR
TEFQFLVSATLPARERQQRLYRDVLDAAGDRPVIFRTVDIGGDKALPYMNVGEGAQEENPAMGWRALRLALDREGLLKVQ
ARALMEAAAGRTLNVMFPMVSEPWEYEAARALFVGQRAWLASHNKKLPVAIRYGAMLEVPGLVETLDLMLPHLDFLSIGT
NDLTQFLFAADRAHPRLAERYDWLSPTVMRYLARVVKIVSGSKVALGVCGEMGGRPLEAMALLGLGIERLSITPAGVGPV
KAMIRSLDLGALRADMPAMLAQPAPDPRGQYEQWALAHQVDLGT

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=742, Percent_Identity=33.1536388140162, Blast_Score=335, Evalue=9e-93,
Organism=Escherichia coli, GI1788756, Length=531, Percent_Identity=33.7099811676083, Blast_Score=298, Evalue=9e-82,
Organism=Escherichia coli, GI1788726, Length=580, Percent_Identity=30.5172413793103, Blast_Score=254, Evalue=2e-68,
Organism=Escherichia coli, GI48994992, Length=478, Percent_Identity=29.2887029288703, Blast_Score=222, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83963; Mature: 83832

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAPPPPSTAAQSARTILTRLHEVMASRANAQGKLNQVVGIIGECLDSEVCSIYLLRDG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHEEEEEEECC
ALELYATRGLKQEAVHVTRLAPGEGLVGTIAEHIETLNLDEAAAHPDFSYRPETGEELFH
CEEEHHHCCCHHHHEEEEEECCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHH
SFAGVPIIRRERAVGVLCVQHAEPRRYEEIEIETLQTVAMVLSELIANADLVDTAARTDA
HHCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AAADQSAQRLNGQKLVDGMGAGVAVFHQPRITIEHTVADDVEAERHRVYAAFDKMREQID
HHHHHHHHHCCCHHHHHCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RMASQAEFGVGGEHEEVLETYKMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMR
HHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QIDDPLLRDRMHDLEDLSNRLIRIVSGQMGTAAQMGLRQDSILIARNLGPAELLEYDRRR
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEECCCHHHHHHHHHHH
LKGVVLEEGSLTAHVIIVARAMGVPVIGRVRDVRTSIREGDLLLLDASAGTVHVRPTPAV
HCEEEEECCCCEEEHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCHHH
QEAFNAKLAISQKRRADLAALRDLPAVTRDGVPIELMINAGLREDVAALDLTGARGIGLF
HHHHCCCEEECHHHHHHHHHHHHCCCHHCCCCCEEEEECCCCCHHHHHEECCCCCCCCHH
RTEFQFLVSATLPARERQQRLYRDVLDAAGDRPVIFRTVDIGGDKALPYMNVGEGAQEEN
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
PAMGWRALRLALDREGLLKVQARALMEAAAGRTLNVMFPMVSEPWEYEAARALFVGQRAW
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCHHH
LASHNKKLPVAIRYGAMLEVPGLVETLDLMLPHLDFLSIGTNDLTQFLFAADRAHPRLAE
HHCCCCCCCEEEECCCEEECCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCHHHHH
RYDWLSPTVMRYLARVVKIVSGSKVALGVCGEMGGRPLEAMALLGLGIERLSITPAGVGP
HHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCHHHEEECCCCCCH
VKAMIRSLDLGALRADMPAMLAQPAPDPRGQYEQWALAHQVDLGT
HHHHHHHCCHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNAPPPPSTAAQSARTILTRLHEVMASRANAQGKLNQVVGIIGECLDSEVCSIYLLRDG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHEEEEEEECC
ALELYATRGLKQEAVHVTRLAPGEGLVGTIAEHIETLNLDEAAAHPDFSYRPETGEELFH
CEEEHHHCCCHHHHEEEEEECCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHH
SFAGVPIIRRERAVGVLCVQHAEPRRYEEIEIETLQTVAMVLSELIANADLVDTAARTDA
HHCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AAADQSAQRLNGQKLVDGMGAGVAVFHQPRITIEHTVADDVEAERHRVYAAFDKMREQID
HHHHHHHHHCCCHHHHHCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RMASQAEFGVGGEHEEVLETYKMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMR
HHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QIDDPLLRDRMHDLEDLSNRLIRIVSGQMGTAAQMGLRQDSILIARNLGPAELLEYDRRR
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEECCCHHHHHHHHHHH
LKGVVLEEGSLTAHVIIVARAMGVPVIGRVRDVRTSIREGDLLLLDASAGTVHVRPTPAV
HCEEEEECCCCEEEHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCHHH
QEAFNAKLAISQKRRADLAALRDLPAVTRDGVPIELMINAGLREDVAALDLTGARGIGLF
HHHHCCCEEECHHHHHHHHHHHHCCCHHCCCCCEEEEECCCCCHHHHHEECCCCCCCCHH
RTEFQFLVSATLPARERQQRLYRDVLDAAGDRPVIFRTVDIGGDKALPYMNVGEGAQEEN
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
PAMGWRALRLALDREGLLKVQARALMEAAAGRTLNVMFPMVSEPWEYEAARALFVGQRAW
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCHHH
LASHNKKLPVAIRYGAMLEVPGLVETLDLMLPHLDFLSIGTNDLTQFLFAADRAHPRLAE
HHCCCCCCCEEEECCCEEECCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCHHHHH
RYDWLSPTVMRYLARVVKIVSGSKVALGVCGEMGGRPLEAMALLGLGIERLSITPAGVGP
HHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCHHHEEECCCCCCH
VKAMIRSLDLGALRADMPAMLAQPAPDPRGQYEQWALAHQVDLGT
HHHHHHHCCHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]