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The map label for this gene is lysC [H]

Identifier: 103487625

GI number: 103487625

Start: 2251144

End: 2252391

Strand: Direct

Name: lysC [H]

Synonym: Sala_2144

Alternate gene names: 103487625

Gene position: 2251144-2252391 (Clockwise)

Preceding gene: 103487623

Following gene: 103487628

Centisome position: 67.3

GC content: 65.54

Gene sequence:

>1248_bases
ATGGCACGGATCGTGATGAAATTCGGGGGCACGTCGATGGCGGGCACCGAGCGGATTCGCACCGTGGCGCGGCTTGTCGC
GCGCGAAGTCGCCGATGGCAATGAGGTGGCGGTCGTGGTCTCGGCCATGGCGGGCGAAACCGACCGGCTGGTGGGTTTCT
GCCGCGAAGCCAACCCGCGCTACGACCCCGCCGAATATGATGTCGTCGTTGCGTCGGGCGAACAGGTCACGTCGGGCCTG
CTTGCGCTGACCCTGCAGGCAATGGGCGTCCCCGCGCGCAGCTGGCTTGGCTGGCAGTTGCCGATCCGCACCGAGGAAGC
GCACGCGCGCGCGCGCATCGCCGACATCGACACCGGCGCGCTCGGCGCCGCGATGGCGAAGGGCGAGGTTGCGGTGATCC
CCGGTTTCCAGGGCATGATGGACGATGGCCGCATCTCGACATTGGGCCGCGGCGGGTCGGACACCAGCGCCGTCGCCGTC
GCCGCAGCAATCAAGGCCGACCGCTGCGACATCTATACCGACGTCGACGGCGTCTATACCACCGATCCGCGCATCGTCGC
GCGCGCGCGCAAGCTCGACTATGTCACCTATGAAGAAATGCTCGAACTCGCGAGCGTCGGCGCGAAAGTGCTCCAGACGC
GCTCGGTCGGACTCGCGATGAAGATGGGCGTGCGCGTGCAGGTCCTGTCCAGCTTCGTCGAGGGCGACGAGGCCCCCAAA
AAAGGCACGATGATCGTCAGCGACGAGGAAATAGAGGAACATCAGATGGAACGGCAGCTGATCACCGGCATCGCCCACGA
CAAGAATGAAGCGAAGATCATCGTCACGCGCGTGCCCGACAGGCCCGGCGCGGTCGCCAACATCTTTGGCCCGCTCGCCG
CCGCGGGGATCAATGTCGACATGATCATCCAGAATGTCGGGCGCGAAAAGGGCGAGACCGACGTGACCTTCACCGTGCCC
GGCGCCGACCTCCTCCGCTCGATCGACCTGCTCGAGAGCGCAAAGGACAAGATCGGCTTCAACCGCGTCATCAGCGACGA
CAAGGTGGCGAAGGTCAGCGTCGTCGGCGTCGGCATGAAAAGCCACGCGGGCGTCGCCAGCACGATGTTCCGCGCACTGG
CGGATCGCGGCATCAACATTCAGGCGATCTCGACCAGCGAGATCAAGGTCAGCGTGCTGATCGACGAGGATGAAACCGAA
CTCGCGGTGCGCGTGCTGCACACCGCCTATGGGCTCGACGCCGAGTAG

Upstream 100 bases:

>100_bases
CTCGCGCTTGCCATCGCCCTTCCCCTTCCGTAACGACGCGAGCGCCGCAGAAATGCTTGGAAAAGTTGCGGCCGAAACCA
GTTCGGGAAAGCGGGGCGAG

Downstream 100 bases:

>100_bases
ACAACGTCATTGCCAGCGCAGCGAAGCAATCTCCAGTTCGATGGCTGGAGATTGCTTCGCTGCGCTCGCCATGACGAAAG
CGCTACTCGGCAACCGCCGC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MARIVMKFGGTSMAGTERIRTVARLVAREVADGNEVAVVVSAMAGETDRLVGFCREANPRYDPAEYDVVVASGEQVTSGL
LALTLQAMGVPARSWLGWQLPIRTEEAHARARIADIDTGALGAAMAKGEVAVIPGFQGMMDDGRISTLGRGGSDTSAVAV
AAAIKADRCDIYTDVDGVYTTDPRIVARARKLDYVTYEEMLELASVGAKVLQTRSVGLAMKMGVRVQVLSSFVEGDEAPK
KGTMIVSDEEIEEHQMERQLITGIAHDKNEAKIIVTRVPDRPGAVANIFGPLAAAGINVDMIIQNVGREKGETDVTFTVP
GADLLRSIDLLESAKDKIGFNRVISDDKVAKVSVVGVGMKSHAGVASTMFRALADRGINIQAISTSEIKVSVLIDEDETE
LAVRVLHTAYGLDAE

Sequences:

>Translated_415_residues
MARIVMKFGGTSMAGTERIRTVARLVAREVADGNEVAVVVSAMAGETDRLVGFCREANPRYDPAEYDVVVASGEQVTSGL
LALTLQAMGVPARSWLGWQLPIRTEEAHARARIADIDTGALGAAMAKGEVAVIPGFQGMMDDGRISTLGRGGSDTSAVAV
AAAIKADRCDIYTDVDGVYTTDPRIVARARKLDYVTYEEMLELASVGAKVLQTRSVGLAMKMGVRVQVLSSFVEGDEAPK
KGTMIVSDEEIEEHQMERQLITGIAHDKNEAKIIVTRVPDRPGAVANIFGPLAAAGINVDMIIQNVGREKGETDVTFTVP
GADLLRSIDLLESAKDKIGFNRVISDDKVAKVSVVGVGMKSHAGVASTMFRALADRGINIQAISTSEIKVSVLIDEDETE
LAVRVLHTAYGLDAE
>Mature_414_residues
ARIVMKFGGTSMAGTERIRTVARLVAREVADGNEVAVVVSAMAGETDRLVGFCREANPRYDPAEYDVVVASGEQVTSGLL
ALTLQAMGVPARSWLGWQLPIRTEEAHARARIADIDTGALGAAMAKGEVAVIPGFQGMMDDGRISTLGRGGSDTSAVAVA
AAIKADRCDIYTDVDGVYTTDPRIVARARKLDYVTYEEMLELASVGAKVLQTRSVGLAMKMGVRVQVLSSFVEGDEAPKK
GTMIVSDEEIEEHQMERQLITGIAHDKNEAKIIVTRVPDRPGAVANIFGPLAAAGINVDMIIQNVGREKGETDVTFTVPG
ADLLRSIDLLESAKDKIGFNRVISDDKVAKVSVVGVGMKSHAGVASTMFRALADRGINIQAISTSEIKVSVLIDEDETEL
AVRVLHTAYGLDAE

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=361, Percent_Identity=31.0249307479224, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790455, Length=466, Percent_Identity=27.6824034334764, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790376, Length=215, Percent_Identity=29.3023255813954, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320893, Length=298, Percent_Identity=30.2013422818792, Blast_Score=114, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44230; Mature: 44099

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIVMKFGGTSMAGTERIRTVARLVAREVADGNEVAVVVSAMAGETDRLVGFCREANPR
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCC
YDPAEYDVVVASGEQVTSGLLALTLQAMGVPARSWLGWQLPIRTEEAHARARIADIDTGA
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCEECCCCCCHHHHHEEEEECCCCC
LGAAMAKGEVAVIPGFQGMMDDGRISTLGRGGSDTSAVAVAAAIKADRCDIYTDVDGVYT
HHHHHCCCCEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCEEE
TDPRIVARARKLDYVTYEEMLELASVGAKVLQTRSVGLAMKMGVRVQVLSSFVEGDEAPK
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCC
KGTMIVSDEEIEEHQMERQLITGIAHDKNEAKIIVTRVPDRPGAVANIFGPLAAAGINVD
CCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHH
MIIQNVGREKGETDVTFTVPGADLLRSIDLLESAKDKIGFNRVISDDKVAKVSVVGVGMK
HHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEEEEECCC
SHAGVASTMFRALADRGINIQAISTSEIKVSVLIDEDETELAVRVLHTAYGLDAE
CCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARIVMKFGGTSMAGTERIRTVARLVAREVADGNEVAVVVSAMAGETDRLVGFCREANPR
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCC
YDPAEYDVVVASGEQVTSGLLALTLQAMGVPARSWLGWQLPIRTEEAHARARIADIDTGA
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCEECCCCCCHHHHHEEEEECCCCC
LGAAMAKGEVAVIPGFQGMMDDGRISTLGRGGSDTSAVAVAAAIKADRCDIYTDVDGVYT
HHHHHCCCCEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCEEE
TDPRIVARARKLDYVTYEEMLELASVGAKVLQTRSVGLAMKMGVRVQVLSSFVEGDEAPK
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCC
KGTMIVSDEEIEEHQMERQLITGIAHDKNEAKIIVTRVPDRPGAVANIFGPLAAAGINVD
CCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHH
MIIQNVGREKGETDVTFTVPGADLLRSIDLLESAKDKIGFNRVISDDKVAKVSVVGVGMK
HHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEEEEECCC
SHAGVASTMFRALADRGINIQAISTSEIKVSVLIDEDETELAVRVLHTAYGLDAE
CCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]