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The map label for this gene is gltD [H]

Identifier: 103487619

GI number: 103487619

Start: 2241517

End: 2242959

Strand: Direct

Name: gltD [H]

Synonym: Sala_2138

Alternate gene names: 103487619

Gene position: 2241517-2242959 (Clockwise)

Preceding gene: 103487618

Following gene: 103487620

Centisome position: 67.01

GC content: 67.84

Gene sequence:

>1443_bases
ATGGTTTCCGAACGCATGCTCCAGTTTGTGGGGCGTGAACAATCCTATCCGGACAAGCGCTCGCCCGAGGAGCGCGCGCG
CGACTTTCGCGAGATCGCCGAGCGCTATTCGGCGCCCGATGCCGACGCGCAGGCGGCGCGCTGCTCGCAATGCGGCGTGC
CCTATTGCTCGGTGCATTGCCCCTTGCACAACCACATCCCCGACTGGCTGCGCCTGACCGCCGAGGGCCGGCTGCGCGAG
GCCTATGAGCTGAGCAACGCGACCTCGACGATGCCCGAGATCTGCGGCCGCATCTGCCCGCAGGACCGCCTGTGCGAGGG
CAATTGCGTCATCGAATTTTCAGGGCATGGCGCGGTGACGATCGGCAGCGTCGAAAAATATATCACCGACACCGCCTGGG
CCGAGGGCTGGGTCGAACCGTTGCGCGTCGGCCCGGCGACCGGCCAGTCGGTCGGCGTGATCGGCGCGGGCCCCGCGGGC
CTCACCGCCGCCGAATATCTGCGCGCCGCTGGGCACGACGTGCATGTCTACGACCGCCACGACCGCGCGGGCGGGCTGCT
GACCTATGGCATCCCCGGCTTCAAGCTGGAGAAGGATGTGGTGATGCGCCGCATCGACCGGCTGGCGGCGGGCGGCATCC
AGTTCCACCTGGGGTTCGAGGTCGGCAAGGACGCGACGCTCGACGGGCTGCGGCAAAAGCATGACGCGATCCTGATCGCG
ACCGGCGTGTACAAGGCGCGCGAGATCAACGTCCCCGGCAACGAGGCCGAAGGCGTGATCGCCGCGCTCGACTATCTGGT
CGCGTCGAACCGCAAGGGTTTCGGCGATGCGGTTCCCGCCTTCGACGACGGCCGCCTGAACGCCGCAGGCAAGCATGTCG
TCGTGATCGGCGGCGGCGACACCGCGATGGACTGCGTCCGCACCGCGGTGCGGCAGGGTGCCAGGTCGGTGAAATGCCTC
TATCGCCGCGACCGCGAAAATATGCCGGGGTCGCAACGCGAGGTCGCCAATGCCGAAGAGGAAGGCGTCGAGTTCGTGTG
GCTCTCCGCTCCCGAAAGCTTCACCGCCGACAGGCATGTCAAAGAGGTCGCGGTCGCAGGGATGCGGCTTGGCGCCCCCG
ATGCGAGCGGCCGCCGCAGCCCCGAGGTCGATCCGGGGCGCAGGTTCGACGTCCCCGCCGACATGGTGATCAAGGCACTG
GGCTTCGATCCCGAGGAATTGCCGCACCTGTTCGGCGCCCCCGACCTCAGCGTCACGCGCTGGGGCACGCTGCGCGTCGA
TCACCAGACGATGATGACCAGCCTGCCCGGCGTGTTCGCCGCCGGCGACATCGTGCGCGGCGCCAGCCTGGTGGTGTGGG
GAATCCGCGACGGCCGCGACGTCAGCGACCAGATGGCAAAGTGGTTGAAGGCGAAAGCGAAAAGCGAAAAGAAGGCAGCA
TGA

Upstream 100 bases:

>100_bases
CGTGTCAGTTTTAGGTTAGCAATGCTGACCTAACTTGCATTTCGGCCGTGACAGCAGCCAGCCGTCTATGCAAGTCCCCA
CGCGACGAAGGGGATATTGC

Downstream 100 bases:

>100_bases
CCAACAGGGGAAGGAAGTTCATGATGTCGTCCTGGAAATATATGCTGCTCGCCGGCGCCGCGGCGCTTGCGCCCGCCGCC
GCCCTCGCCGCGCCAACCGA

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MVSERMLQFVGREQSYPDKRSPEERARDFREIAERYSAPDADAQAARCSQCGVPYCSVHCPLHNHIPDWLRLTAEGRLRE
AYELSNATSTMPEICGRICPQDRLCEGNCVIEFSGHGAVTIGSVEKYITDTAWAEGWVEPLRVGPATGQSVGVIGAGPAG
LTAAEYLRAAGHDVHVYDRHDRAGGLLTYGIPGFKLEKDVVMRRIDRLAAGGIQFHLGFEVGKDATLDGLRQKHDAILIA
TGVYKAREINVPGNEAEGVIAALDYLVASNRKGFGDAVPAFDDGRLNAAGKHVVVIGGGDTAMDCVRTAVRQGARSVKCL
YRRDRENMPGSQREVANAEEEGVEFVWLSAPESFTADRHVKEVAVAGMRLGAPDASGRRSPEVDPGRRFDVPADMVIKAL
GFDPEELPHLFGAPDLSVTRWGTLRVDHQTMMTSLPGVFAAGDIVRGASLVVWGIRDGRDVSDQMAKWLKAKAKSEKKAA

Sequences:

>Translated_480_residues
MVSERMLQFVGREQSYPDKRSPEERARDFREIAERYSAPDADAQAARCSQCGVPYCSVHCPLHNHIPDWLRLTAEGRLRE
AYELSNATSTMPEICGRICPQDRLCEGNCVIEFSGHGAVTIGSVEKYITDTAWAEGWVEPLRVGPATGQSVGVIGAGPAG
LTAAEYLRAAGHDVHVYDRHDRAGGLLTYGIPGFKLEKDVVMRRIDRLAAGGIQFHLGFEVGKDATLDGLRQKHDAILIA
TGVYKAREINVPGNEAEGVIAALDYLVASNRKGFGDAVPAFDDGRLNAAGKHVVVIGGGDTAMDCVRTAVRQGARSVKCL
YRRDRENMPGSQREVANAEEEGVEFVWLSAPESFTADRHVKEVAVAGMRLGAPDASGRRSPEVDPGRRFDVPADMVIKAL
GFDPEELPHLFGAPDLSVTRWGTLRVDHQTMMTSLPGVFAAGDIVRGASLVVWGIRDGRDVSDQMAKWLKAKAKSEKKAA
>Mature_480_residues
MVSERMLQFVGREQSYPDKRSPEERARDFREIAERYSAPDADAQAARCSQCGVPYCSVHCPLHNHIPDWLRLTAEGRLRE
AYELSNATSTMPEICGRICPQDRLCEGNCVIEFSGHGAVTIGSVEKYITDTAWAEGWVEPLRVGPATGQSVGVIGAGPAG
LTAAEYLRAAGHDVHVYDRHDRAGGLLTYGIPGFKLEKDVVMRRIDRLAAGGIQFHLGFEVGKDATLDGLRQKHDAILIA
TGVYKAREINVPGNEAEGVIAALDYLVASNRKGFGDAVPAFDDGRLNAAGKHVVVIGGGDTAMDCVRTAVRQGARSVKCL
YRRDRENMPGSQREVANAEEEGVEFVWLSAPESFTADRHVKEVAVAGMRLGAPDASGRRSPEVDPGRRFDVPADMVIKAL
GFDPEELPHLFGAPDLSVTRWGTLRVDHQTMMTSLPGVFAAGDIVRGASLVVWGIRDGRDVSDQMAKWLKAKAKSEKKAA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=454, Percent_Identity=27.9735682819383, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1789606, Length=476, Percent_Identity=46.218487394958, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1788811, Length=464, Percent_Identity=45.9051724137931, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI87082180, Length=463, Percent_Identity=44.4924406047516, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1789243, Length=445, Percent_Identity=33.2584269662921, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1788468, Length=450, Percent_Identity=28.6666666666667, Blast_Score=164, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17570289, Length=497, Percent_Identity=35.2112676056338, Blast_Score=258, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI71984108, Length=466, Percent_Identity=25.9656652360515, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17543860, Length=161, Percent_Identity=29.1925465838509, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=490, Percent_Identity=35.1020408163265, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=35.5102040816327, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=35.5102040816327, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24665547, Length=497, Percent_Identity=35.2112676056338, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24665543, Length=497, Percent_Identity=35.2112676056338, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24640763, Length=446, Percent_Identity=27.1300448430493, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI18858217, Length=446, Percent_Identity=27.1300448430493, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52132; Mature: 52132

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSERMLQFVGREQSYPDKRSPEERARDFREIAERYSAPDADAQAARCSQCGVPYCSVHC
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEC
PLHNHIPDWLRLTAEGRLREAYELSNATSTMPEICGRICPQDRLCEGNCVIEFSGHGAVT
CHHHCCCHHHEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEE
IGSVEKYITDTAWAEGWVEPLRVGPATGQSVGVIGAGPAGLTAAEYLRAAGHDVHVYDRH
ECCHHHHHHHHHHHHHCHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECC
DRAGGLLTYGIPGFKLEKDVVMRRIDRLAAGGIQFHLGFEVGKDATLDGLRQKHDAILIA
CCCCCEEEECCCCCEECHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCCEEEEE
TGVYKAREINVPGNEAEGVIAALDYLVASNRKGFGDAVPAFDDGRLNAAGKHVVVIGGGD
ECCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
TAMDCVRTAVRQGARSVKCLYRRDRENMPGSQREVANAEEEGVEFVWLSAPESFTADRHV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHCCCEEEEEECCCCCCHHHHH
KEVAVAGMRLGAPDASGRRSPEVDPGRRFDVPADMVIKALGFDPEELPHLFGAPDLSVTR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEE
WGTLRVDHQTMMTSLPGVFAAGDIVRGASLVVWGIRDGRDVSDQMAKWLKAKAKSEKKAA
CEEEEECHHHHHHHCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVSERMLQFVGREQSYPDKRSPEERARDFREIAERYSAPDADAQAARCSQCGVPYCSVHC
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEC
PLHNHIPDWLRLTAEGRLREAYELSNATSTMPEICGRICPQDRLCEGNCVIEFSGHGAVT
CHHHCCCHHHEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEE
IGSVEKYITDTAWAEGWVEPLRVGPATGQSVGVIGAGPAGLTAAEYLRAAGHDVHVYDRH
ECCHHHHHHHHHHHHHCHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECC
DRAGGLLTYGIPGFKLEKDVVMRRIDRLAAGGIQFHLGFEVGKDATLDGLRQKHDAILIA
CCCCCEEEECCCCCEECHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCCEEEEE
TGVYKAREINVPGNEAEGVIAALDYLVASNRKGFGDAVPAFDDGRLNAAGKHVVVIGGGD
ECCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
TAMDCVRTAVRQGARSVKCLYRRDRENMPGSQREVANAEEEGVEFVWLSAPESFTADRHV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHCCCEEEEEECCCCCCHHHHH
KEVAVAGMRLGAPDASGRRSPEVDPGRRFDVPADMVIKALGFDPEELPHLFGAPDLSVTR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEE
WGTLRVDHQTMMTSLPGVFAAGDIVRGASLVVWGIRDGRDVSDQMAKWLKAKAKSEKKAA
CEEEEECHHHHHHHCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8428988; 2198943 [H]