Click here to switch to the map view.

The map label for this gene is leuC

Identifier: 103487610

GI number: 103487610

Start: 2233902

End: 2235332

Strand: Direct

Name: leuC

Synonym: Sala_2129

Alternate gene names: 103487610

Gene position: 2233902-2235332 (Clockwise)

Preceding gene: 103487608

Following gene: 103487611

Centisome position: 66.78

GC content: 68.34

Gene sequence:

>1431_bases
ATGACTCGGCCCCGCACGCTTTACGAAAAAATCTGGGACGCGCATGTCGTCGAACGGCGCGGCGACGGCACCTGCCTGAT
CTTCATCGACCGCCACCTCGTCCATGAAGTCACCAGCCCGCAGGCCTTTGCCGGGCTTCGCGCGAGCGGCCGCACGGTGC
GCCGCCCCGACCTGACGCTCGCGGTGCCCGACCATAATGTGCCGACGACGCCGCGCAAGGATGCGGCGGGCAACCGGCTG
CCGATCGCCGACCCCGAAAGCGCGGCGCAATTGGCGGCGCTGGAGAAGAACGCGCCCGAGTTCGGCATCCGTTACATCGA
CGCGATCGCGCCCGAGCAGGGCATCGTTCATGTCGTCGGCCCCGAACAGGGTTTTTCGCTGCCCGGCGCAACGATCGTTT
GCGGCGACAGCCACACGGCGTGCCACGGCGGCATCGGCGCGCTCGCCTTTGGCATCGGGACGAGCGAGGTCGAGCATGTT
CTGGCGACGCAGACCCTGCTGCTCCAGCCCGCGAAGACGATGGAGGTGCGCGTCGAGGGCGACGTCGGCCCCGGCGTCAG
CGCGAAGGACATCATCCTGCACATCACGGGCACCATCGGCGCCGCGGGCGGCACGGGCCACGTCATCGAATATACCGGCA
GCGCGATCCGCGCGCTGTCGATCGAGGGGCGGCTGACGATCAGCAACATGGCGATCGAGGGCGGCGCGCGCGCCGGACTG
ATCGCGCCCGACGAGACGACTTTCGCTTATCTCAAGGGCCGCCCCTACGCCCCGAAGGGCGCCGACTGGGACGCCGCGGT
CGCTTACTGGAAAAGCCTGACCACCGATCCCGGCGCGACTTACGACAAGGTGGTCGTCATCGACGCCGCCGACATCGCGC
CCAGCGTGACGTGGGGCACGAGTCCCGAGGACGTCGTGCCGATCACCGGCACCGTGCCCGATCCGGCAAGCTTTTCCGAT
CCGTCGAAACGGGCCGCCGCCGCCAAGAGCCTGGCCTATATGGGGCTCGAACCCGGCACGCGGATGCAGGATGTGCCGGT
CGAAAATATCTTCATCGGCAGCTGCACCAACAGCCGGATCGAGGATTTGCGCGCCGCCGCAGCGGTGCTGAAGGGTCGCA
GGAAGGCGCCGGGCGTCAAATGGGCGATCGTCGTTCCCGGTTCGGGCCTGGTGAAGGCTCAGGCCGAGGCCGAGGGACTC
GACCGCATCTTCATCGACGCGGGACTCGAATGGCGCGAGCCCGGCTGTTCGGCGTGCCTCGCGATGAACCCCGACAAGGT
GCCCGCGGGCGAGCGCTGCGCATCGACGAGCAATCGCAATTTCGTCGGTCGGCAAGGACCGGGTGCCCGCACGCACCTCG
TCAGCCCCGCGATGGCCGCGGCGGCGGCGGTGACCGGAAAGCTCACCGACGTGCGCGAATTAATGGCATGA

Upstream 100 bases:

>100_bases
AGAGAGGGCTGGGCACGGCGCGCGGGCCATTGCGACCGAAAATTGCGTTTTCCTTCGCCACTCGCTTGCAAAGTTATGCA
GGCGGCGTAAGTGATCCCGC

Downstream 100 bases:

>100_bases
ACGCAGACAGAGGGAGCCGAAGGGCATGATCGGGAAAGCTGTCATAACGATCGCGGCGGCACTGGCGCTCGCCACCCCCG
CGCTGGCGCAGGACAGGCCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTRPRTLYEKIWDAHVVERRGDGTCLIFIDRHLVHEVTSPQAFAGLRASGRTVRRPDLTLAVPDHNVPTTPRKDAAGNRL
PIADPESAAQLAALEKNAPEFGIRYIDAIAPEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGIGALAFGIGTSEVEHV
LATQTLLLQPAKTMEVRVEGDVGPGVSAKDIILHITGTIGAAGGTGHVIEYTGSAIRALSIEGRLTISNMAIEGGARAGL
IAPDETTFAYLKGRPYAPKGADWDAAVAYWKSLTTDPGATYDKVVVIDAADIAPSVTWGTSPEDVVPITGTVPDPASFSD
PSKRAAAAKSLAYMGLEPGTRMQDVPVENIFIGSCTNSRIEDLRAAAAVLKGRRKAPGVKWAIVVPGSGLVKAQAEAEGL
DRIFIDAGLEWREPGCSACLAMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGKLTDVRELMA

Sequences:

>Translated_476_residues
MTRPRTLYEKIWDAHVVERRGDGTCLIFIDRHLVHEVTSPQAFAGLRASGRTVRRPDLTLAVPDHNVPTTPRKDAAGNRL
PIADPESAAQLAALEKNAPEFGIRYIDAIAPEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGIGALAFGIGTSEVEHV
LATQTLLLQPAKTMEVRVEGDVGPGVSAKDIILHITGTIGAAGGTGHVIEYTGSAIRALSIEGRLTISNMAIEGGARAGL
IAPDETTFAYLKGRPYAPKGADWDAAVAYWKSLTTDPGATYDKVVVIDAADIAPSVTWGTSPEDVVPITGTVPDPASFSD
PSKRAAAAKSLAYMGLEPGTRMQDVPVENIFIGSCTNSRIEDLRAAAAVLKGRRKAPGVKWAIVVPGSGLVKAQAEAEGL
DRIFIDAGLEWREPGCSACLAMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGKLTDVRELMA
>Mature_475_residues
TRPRTLYEKIWDAHVVERRGDGTCLIFIDRHLVHEVTSPQAFAGLRASGRTVRRPDLTLAVPDHNVPTTPRKDAAGNRLP
IADPESAAQLAALEKNAPEFGIRYIDAIAPEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGIGALAFGIGTSEVEHVL
ATQTLLLQPAKTMEVRVEGDVGPGVSAKDIILHITGTIGAAGGTGHVIEYTGSAIRALSIEGRLTISNMAIEGGARAGLI
APDETTFAYLKGRPYAPKGADWDAAVAYWKSLTTDPGATYDKVVVIDAADIAPSVTWGTSPEDVVPITGTVPDPASFSDP
SKRAAAAKSLAYMGLEPGTRMQDVPVENIFIGSCTNSRIEDLRAAAAVLKGRRKAPGVKWAIVVPGSGLVKAQAEAEGLD
RIFIDAGLEWREPGCSACLAMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGKLTDVRELMA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=489, Percent_Identity=27.4028629856851, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI8659555, Length=440, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI41352693, Length=445, Percent_Identity=25.3932584269663, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786259, Length=471, Percent_Identity=64.755838641189, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=25.5494505494505, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081781, Length=358, Percent_Identity=25.6983240223464, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367097, Length=395, Percent_Identity=26.5822784810127, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=492, Percent_Identity=29.6747967479675, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=368, Percent_Identity=31.25, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=311, Percent_Identity=30.8681672025724, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=466, Percent_Identity=27.2532188841202, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=60.7218683651805, Blast_Score=605, Evalue=1e-174,
Organism=Saccharomyces cerevisiae, GI6320440, Length=466, Percent_Identity=27.4678111587983, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=367, Percent_Identity=31.0626702997275, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=485, Percent_Identity=27.6288659793814, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281365315, Length=493, Percent_Identity=27.5862068965517, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17864292, Length=493, Percent_Identity=27.5862068965517, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076999, Length=367, Percent_Identity=29.9727520435967, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=366, Percent_Identity=28.9617486338798, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645686, Length=455, Percent_Identity=26.3736263736264, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI17137564, Length=387, Percent_Identity=25.5813953488372, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SPHAL (Q1GR84)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617171.1
- ProteinModelPortal:   Q1GR84
- SMR:   Q1GR84
- GeneID:   4080104
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2129
- NMPDR:   fig|317655.9.peg.2056
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   SALA317655:SALA_2129-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 49846; Mature: 49715

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPRTLYEKIWDAHVVERRGDGTCLIFIDRHLVHEVTSPQAFAGLRASGRTVRRPDLTL
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCHHHHCCCCCCCEEECCCEEE
AVPDHNVPTTPRKDAAGNRLPIADPESAAQLAALEKNAPEFGIRYIDAIAPEQGIVHVVG
EECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEC
PEQGFSLPGATIVCGDSHTACHGGIGALAFGIGTSEVEHVLATQTLLLQPAKTMEVRVEG
CCCCCCCCCCEEEECCCCCCCCCCHHHEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEC
DVGPGVSAKDIILHITGTIGAAGGTGHVIEYTGSAIRALSIEGRLTISNMAIEGGARAGL
CCCCCCCCCEEEEEEEECEECCCCCCCEEEECCCEEEEEEECCEEEEEEEEECCCCCCCE
IAPDETTFAYLKGRPYAPKGADWDAAVAYWKSLTTDPGATYDKVVVIDAADIAPSVTWGT
ECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCC
SPEDVVPITGTVPDPASFSDPSKRAAAAKSLAYMGLEPGTRMQDVPVENIFIGSCTNSRI
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEECCCCHHH
EDLRAAAAVLKGRRKAPGVKWAIVVPGSGLVKAQAEAEGLDRIFIDAGLEWREPGCSACL
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCCCEEEEECCCCCCCCCCCEEE
AMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGKLTDVRELMA
EECCCCCCCCCHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHC
>Mature Secondary Structure 
TRPRTLYEKIWDAHVVERRGDGTCLIFIDRHLVHEVTSPQAFAGLRASGRTVRRPDLTL
CCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCHHHHCCCCCCCEEECCCEEE
AVPDHNVPTTPRKDAAGNRLPIADPESAAQLAALEKNAPEFGIRYIDAIAPEQGIVHVVG
EECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEC
PEQGFSLPGATIVCGDSHTACHGGIGALAFGIGTSEVEHVLATQTLLLQPAKTMEVRVEG
CCCCCCCCCCEEEECCCCCCCCCCHHHEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEC
DVGPGVSAKDIILHITGTIGAAGGTGHVIEYTGSAIRALSIEGRLTISNMAIEGGARAGL
CCCCCCCCCEEEEEEEECEECCCCCCCEEEECCCEEEEEEECCEEEEEEEEECCCCCCCE
IAPDETTFAYLKGRPYAPKGADWDAAVAYWKSLTTDPGATYDKVVVIDAADIAPSVTWGT
ECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCC
SPEDVVPITGTVPDPASFSDPSKRAAAAKSLAYMGLEPGTRMQDVPVENIFIGSCTNSRI
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEECCCCHHH
EDLRAAAAVLKGRRKAPGVKWAIVVPGSGLVKAQAEAEGLDRIFIDAGLEWREPGCSACL
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCCCEEEEECCCCCCCCCCCEEE
AMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGKLTDVRELMA
EECCCCCCCCCHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA