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The map label for this gene is trmH [C]

Identifier: 103487534

GI number: 103487534

Start: 2162452

End: 2163264

Strand: Direct

Name: trmH [C]

Synonym: Sala_2053

Alternate gene names: 103487534

Gene position: 2162452-2163264 (Clockwise)

Preceding gene: 103487533

Following gene: 103487535

Centisome position: 64.64

GC content: 69.62

Gene sequence:

>813_bases
ATGACCGGTCGCCGCTCCCAAATCGAAAGCCTGTCGAACCCGCTGGTCAAGCGGATGCGCCTGCTGCGCGAGAAGCGCCA
TCGCCGCGCCGAAGGGCTGTTTCTGGCCGAAGGGCTGCGCATCGCGACCGAGGCGCGCGAGGCGGGCGTCTTTCCCGCGT
GGCTGTTCCTGGGCCCCGACGGCGCGGCGCATCCGCTGGCGCAGGCGCTGGTCGCGGACACGATGGCCGCGGGCGGCGAG
GTGATCGACACCACAGCCGCGATCCTCTCCAAATTGTCGGGCAAGGACAATCCGCAGACGATCGTCGGCATCTATGCCGA
ACCCGCCACGACACTCGCTGCCATCGACCGCAACGCCGCGCCGATCTGGCTCGTCGCCGAACGCCTCCGCGATCCCGGCA
ATCTCGGCACGATGCTGCGCACCGGCGACGCGGTCGGCGCGGGCGGGCTGATTCTCCTGGGCGACAGCACCGACCCCTAT
GGCGTCGAGGCGGTGCGCGCGAGCATGGGGGCGATCTTTACGCAAAGGCTGGTGCAGGCGCGCTGGGACGCGTTCCTGCC
CTGGCTGCGCAGCGGACAGGGCGAGCTGGTCGCGACCTGGCTCGGCGACGACAGCGTCGATTATCAGGCGGTGCGCTATG
CCGCGCCAACCTTTATCCTCGTCGGCAATGAATCGCAAGGGCTGCCCGCCGATTATGCCGCGGCGGCCGACGTGCGCGTC
AAGATGCCGATGCTGGGCAAGGCCGACAGCCTGAACGCCGCCGTCGCGGCGGCGGTGATGGCCTATGAGGTGCTGAACCA
GCGGCGGCGCTGA

Upstream 100 bases:

>100_bases
CGACACGATCACCATCCACACGCGCGGCGACGGCGCCGACGCCGCACTCCTGAAACTCGTCGGGCTGGTCAAGGACAGCT
TTGGCGAGGACTAGGGCGCG

Downstream 100 bases:

>100_bases
ACGACGAGCGGCATTTTGGTTCAGCCGCCCGCCAGCATCATGCTATTATCTCATCCTCTCTTCCCGTTCGCATCGAGCGA
AGTCGAGATGGCGCTCGACC

Product: tRNA/rRNA methyltransferase (SpoU)

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTGRRSQIESLSNPLVKRMRLLREKRHRRAEGLFLAEGLRIATEAREAGVFPAWLFLGPDGAAHPLAQALVADTMAAGGE
VIDTTAAILSKLSGKDNPQTIVGIYAEPATTLAAIDRNAAPIWLVAERLRDPGNLGTMLRTGDAVGAGGLILLGDSTDPY
GVEAVRASMGAIFTQRLVQARWDAFLPWLRSGQGELVATWLGDDSVDYQAVRYAAPTFILVGNESQGLPADYAAAADVRV
KMPMLGKADSLNAAVAAAVMAYEVLNQRRR

Sequences:

>Translated_270_residues
MTGRRSQIESLSNPLVKRMRLLREKRHRRAEGLFLAEGLRIATEAREAGVFPAWLFLGPDGAAHPLAQALVADTMAAGGE
VIDTTAAILSKLSGKDNPQTIVGIYAEPATTLAAIDRNAAPIWLVAERLRDPGNLGTMLRTGDAVGAGGLILLGDSTDPY
GVEAVRASMGAIFTQRLVQARWDAFLPWLRSGQGELVATWLGDDSVDYQAVRYAAPTFILVGNESQGLPADYAAAADVRV
KMPMLGKADSLNAAVAAAVMAYEVLNQRRR
>Mature_269_residues
TGRRSQIESLSNPLVKRMRLLREKRHRRAEGLFLAEGLRIATEAREAGVFPAWLFLGPDGAAHPLAQALVADTMAAGGEV
IDTTAAILSKLSGKDNPQTIVGIYAEPATTLAAIDRNAAPIWLVAERLRDPGNLGTMLRTGDAVGAGGLILLGDSTDPYG
VEAVRASMGAIFTQRLVQARWDAFLPWLRSGQGELVATWLGDDSVDYQAVRYAAPTFILVGNESQGLPADYAAAADVRVK
MPMLGKADSLNAAVAAAVMAYEVLNQRRR

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=305, Percent_Identity=24.5901639344262, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1790083, Length=166, Percent_Identity=30.1204819277108, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 28865; Mature: 28734

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRRSQIESLSNPLVKRMRLLREKRHRRAEGLFLAEGLRIATEAREAGVFPAWLFLGPD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHCCCHHHHHHHHCCCCEEEEEECCC
GAAHPLAQALVADTMAAGGEVIDTTAAILSKLSGKDNPQTIVGIYAEPATTLAAIDRNAA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCCC
PIWLVAERLRDPGNLGTMLRTGDAVGAGGLILLGDSTDPYGVEAVRASMGAIFTQRLVQA
CHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
RWDAFLPWLRSGQGELVATWLGDDSVDYQAVRYAAPTFILVGNESQGLPADYAAAADVRV
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHEECCCEEEEECCCCCCCCCCHHHHCCEEE
KMPMLGKADSLNAAVAAAVMAYEVLNQRRR
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGRRSQIESLSNPLVKRMRLLREKRHRRAEGLFLAEGLRIATEAREAGVFPAWLFLGPD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHCCCHHHHHHHHCCCCEEEEEECCC
GAAHPLAQALVADTMAAGGEVIDTTAAILSKLSGKDNPQTIVGIYAEPATTLAAIDRNAA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCCC
PIWLVAERLRDPGNLGTMLRTGDAVGAGGLILLGDSTDPYGVEAVRASMGAIFTQRLVQA
CHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
RWDAFLPWLRSGQGELVATWLGDDSVDYQAVRYAAPTFILVGNESQGLPADYAAAADVRV
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHEECCCEEEEECCCCCCCCCCHHHHCCEEE
KMPMLGKADSLNAAVAAAVMAYEVLNQRRR
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]