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The map label for this gene is pckA [H]

Identifier: 103487527

GI number: 103487527

Start: 2156127

End: 2157812

Strand: Reverse

Name: pckA [H]

Synonym: Sala_2046

Alternate gene names: 103487527

Gene position: 2157812-2156127 (Counterclockwise)

Preceding gene: 103487536

Following gene: 103487526

Centisome position: 64.51

GC content: 64.29

Gene sequence:

>1686_bases
ATGTTCGTGCGGGCCCATCATTTCCGCACGGCATGTCCTGCATACCTATGCAAAGGCGCCGCGGGCCATGGAAAGGCGAG
TGAAATGACCAAGGTTGTGATCGGCGGCGACACCATCGAAACGCAGGCGGAGGTGGCCGAAAACTGGGGTACCGCGCCGC
TGGTCGAGGAAGCGGTGCGCCACGGCGAAGGCCGGCTCGCCAAGGACGGCCCGCTCGTCGTCGCGACCGGCAAGCACACC
GGGCGCAGCGCGAAAGACAAATTCATCGTCCAGGACGACGAAACGCGCGACACGATCTGGTGGGGCAAGACCAATGTGCC
GATGACGCCCGATCATTTCGCGGCGCTCAAGGCCGATTTCCTCGCGCACATGGCGGCGCGTCCGCGCCTTTACCGCCAAC
AGCTGTTCGGCGGATCGCAGCCGGAGCACCGCGTCGCGGTGCAGGTCATCACCGAATTCGCCTGGCACAGCCATTTCATC
CGCACCCTGCTCTGCCGCCCGACGGCCGAGGAGCTGGCGGGCTTTGCGGCCGAATATACAATCGTCGATCTGCCAAGCTT
CCGCGCCGACCCGGCGAAGCACGGCACGCGCAGCGAAACGGTGATCGCGGTCAATTTCACCGAAAAGCTGATCCTGATCG
GCGGCACGCAATATGCGGGCGAGATGAAAAAGTCGGTGTTCGGCATCCTGAACTATCTGCTCCCGGTAAAGGGGGTGATG
CCGATGCATTGTTCGGCGAATATCGGGCCCAGGGGCGACACCGCGGTCTTTTTCGGCCTCAGCGGCACCGGCAAGACGAC
GCTGTCGGCCGACGCCTCGCGCACCTTGATCGGCGACGACGAGCACGGCTGGTCGGACACCGCGGTCTTCAATTTCGAGG
GCGGCTGCTATGCCAAGATGATCCGCCTCTCGCCCGAGGCCGAGCCGGAGATTTTCGCGACGACCAGGCGTTTCGGCACC
GTGCTCGAAAATGTCGTGATGGACCCGGTGACGCGCGAACTCGATTTCGACGATGCGACCCTCGCCGAGAACAGCCGTGG
CAGCTATCCGATTGATTTCATTCCGAATACATCGGAAAACAATATGGGTCCGGTGCCGCAGAACATCATCATGCTCACCG
CCGATGCCTATGGCGTGCTGCCCCCGATCGCGAAGCTCACCCCCGATCAGGCGATGTATCATTTCCTGTCGGGCTATACC
GCGCGCGTCGCGGGGACCGAAATCGGCGTGACCGAGCCGGAGGCGACCTTCTCGACCTGTTTCGGCGCGCCCTTCATGCC
GCGCCACCCGTCGGTGTACGGCAATCTGCTGAAAGAGCGCATCGCCAGGGGCGGGGTCCAGTGCTGGCTGGTCAACACCG
GCTGGACCGGCGGCATGGCGACGATGGAGGGCATCAAGCGGATGCCGATCAAGGCGACGCGCGCGCTGCTCAATGCGGCG
CTCGACGGCAGCCTGAACGACGCCGAGTTCCGCCGCGATCGCTATTTCGGCTTCATGGTGCCCGTCGCGGTGCCCGGCGT
CGATGCCCGCCTGCTCGACCCCCGCGAAGCCTGGACCGACAAGGCGGCCTATGACCGCACCGCGCAAACGCTGGTGCAGC
AATTCATCGCCAATTTCGCGCAATTTGAAAGCCATGTCGACGAGGGCGTCCGTCAGGCCGCTCCCCAGGTCGCCGTCGCG
GCGTAA

Upstream 100 bases:

>100_bases
CGCACGGTCCGTCGCCGATTCACCCTTTAATCTTTTGACCTCGGCGCGCATCCTCGCTAACGCGGCCGGGATTTCGGAAT
CGGCGTTTCGTCGGCACCCC

Downstream 100 bases:

>100_bases
CTTGCCCGGACAACTTCCGAGTGGCAGATCACCCGGAAGGACCGGACCAATGACCACCGATTTTTCACCGCGCCTGTTCG
AGCGCGATTCCGACATCCGC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MFVRAHHFRTACPAYLCKGAAGHGKASEMTKVVIGGDTIETQAEVAENWGTAPLVEEAVRHGEGRLAKDGPLVVATGKHT
GRSAKDKFIVQDDETRDTIWWGKTNVPMTPDHFAALKADFLAHMAARPRLYRQQLFGGSQPEHRVAVQVITEFAWHSHFI
RTLLCRPTAEELAGFAAEYTIVDLPSFRADPAKHGTRSETVIAVNFTEKLILIGGTQYAGEMKKSVFGILNYLLPVKGVM
PMHCSANIGPRGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSPEAEPEIFATTRRFGT
VLENVVMDPVTRELDFDDATLAENSRGSYPIDFIPNTSENNMGPVPQNIIMLTADAYGVLPPIAKLTPDQAMYHFLSGYT
ARVAGTEIGVTEPEATFSTCFGAPFMPRHPSVYGNLLKERIARGGVQCWLVNTGWTGGMATMEGIKRMPIKATRALLNAA
LDGSLNDAEFRRDRYFGFMVPVAVPGVDARLLDPREAWTDKAAYDRTAQTLVQQFIANFAQFESHVDEGVRQAAPQVAVA
A

Sequences:

>Translated_561_residues
MFVRAHHFRTACPAYLCKGAAGHGKASEMTKVVIGGDTIETQAEVAENWGTAPLVEEAVRHGEGRLAKDGPLVVATGKHT
GRSAKDKFIVQDDETRDTIWWGKTNVPMTPDHFAALKADFLAHMAARPRLYRQQLFGGSQPEHRVAVQVITEFAWHSHFI
RTLLCRPTAEELAGFAAEYTIVDLPSFRADPAKHGTRSETVIAVNFTEKLILIGGTQYAGEMKKSVFGILNYLLPVKGVM
PMHCSANIGPRGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSPEAEPEIFATTRRFGT
VLENVVMDPVTRELDFDDATLAENSRGSYPIDFIPNTSENNMGPVPQNIIMLTADAYGVLPPIAKLTPDQAMYHFLSGYT
ARVAGTEIGVTEPEATFSTCFGAPFMPRHPSVYGNLLKERIARGGVQCWLVNTGWTGGMATMEGIKRMPIKATRALLNAA
LDGSLNDAEFRRDRYFGFMVPVAVPGVDARLLDPREAWTDKAAYDRTAQTLVQQFIANFAQFESHVDEGVRQAAPQVAVA
A
>Mature_561_residues
MFVRAHHFRTACPAYLCKGAAGHGKASEMTKVVIGGDTIETQAEVAENWGTAPLVEEAVRHGEGRLAKDGPLVVATGKHT
GRSAKDKFIVQDDETRDTIWWGKTNVPMTPDHFAALKADFLAHMAARPRLYRQQLFGGSQPEHRVAVQVITEFAWHSHFI
RTLLCRPTAEELAGFAAEYTIVDLPSFRADPAKHGTRSETVIAVNFTEKLILIGGTQYAGEMKKSVFGILNYLLPVKGVM
PMHCSANIGPRGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSPEAEPEIFATTRRFGT
VLENVVMDPVTRELDFDDATLAENSRGSYPIDFIPNTSENNMGPVPQNIIMLTADAYGVLPPIAKLTPDQAMYHFLSGYT
ARVAGTEIGVTEPEATFSTCFGAPFMPRHPSVYGNLLKERIARGGVQCWLVNTGWTGGMATMEGIKRMPIKATRALLNAA
LDGSLNDAEFRRDRYFGFMVPVAVPGVDARLLDPREAWTDKAAYDRTAQTLVQQFIANFAQFESHVDEGVRQAAPQVAVA
A

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=498, Percent_Identity=48.5943775100402, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322950, Length=517, Percent_Identity=44.100580270793, Blast_Score=441, Evalue=1e-124,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 61263; Mature: 61263

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVRAHHFRTACPAYLCKGAAGHGKASEMTKVVIGGDTIETQAEVAENWGTAPLVEEAVR
CCEECCHHHHHHHHHHHCCCCCCCCHHHCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHH
HGEGRLAKDGPLVVATGKHTGRSAKDKFIVQDDETRDTIWWGKTNVPMTPDHFAALKADF
CCCCCEECCCCEEEEECCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHH
LAHMAARPRLYRQQLFGGSQPEHRVAVQVITEFAWHSHFIRTLLCRPTAEELAGFAAEYT
HHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHCEE
IVDLPSFRADPAKHGTRSETVIAVNFTEKLILIGGTQYAGEMKKSVFGILNYLLPVKGVM
EEECCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
PMHCSANIGPRGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKM
CCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCEECCCCCCCCCCEEEEECCCCEEEE
IRLSPEAEPEIFATTRRFGTVLENVVMDPVTRELDFDDATLAENSRGSYPIDFIPNTSEN
EEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHCCCCCCCCCEEECCCCCCC
NMGPVPQNIIMLTADAYGVLPPIAKLTPDQAMYHFLSGYTARVAGTEIGVTEPEATFSTC
CCCCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHH
FGAPFMPRHPSVYGNLLKERIARGGVQCWLVNTGWTGGMATMEGIKRMPIKATRALLNAA
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHH
LDGSLNDAEFRRDRYFGFMVPVAVPGVDARLLDPREAWTDKAAYDRTAQTLVQQFIANFA
HCCCCCHHHHHHHHHCEEEEEEECCCCCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHH
QFESHVDEGVRQAAPQVAVAA
HHHHHHHHHHHHHCCCEEECC
>Mature Secondary Structure
MFVRAHHFRTACPAYLCKGAAGHGKASEMTKVVIGGDTIETQAEVAENWGTAPLVEEAVR
CCEECCHHHHHHHHHHHCCCCCCCCHHHCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHH
HGEGRLAKDGPLVVATGKHTGRSAKDKFIVQDDETRDTIWWGKTNVPMTPDHFAALKADF
CCCCCEECCCCEEEEECCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHH
LAHMAARPRLYRQQLFGGSQPEHRVAVQVITEFAWHSHFIRTLLCRPTAEELAGFAAEYT
HHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHCEE
IVDLPSFRADPAKHGTRSETVIAVNFTEKLILIGGTQYAGEMKKSVFGILNYLLPVKGVM
EEECCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
PMHCSANIGPRGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKM
CCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCEECCCCCCCCCCEEEEECCCCEEEE
IRLSPEAEPEIFATTRRFGTVLENVVMDPVTRELDFDDATLAENSRGSYPIDFIPNTSEN
EEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHCCCCCCCCCEEECCCCCCC
NMGPVPQNIIMLTADAYGVLPPIAKLTPDQAMYHFLSGYTARVAGTEIGVTEPEATFSTC
CCCCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHH
FGAPFMPRHPSVYGNLLKERIARGGVQCWLVNTGWTGGMATMEGIKRMPIKATRALLNAA
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHH
LDGSLNDAEFRRDRYFGFMVPVAVPGVDARLLDPREAWTDKAAYDRTAQTLVQQFIANFA
HCCCCCHHHHHHHHHCEEEEEEECCCCCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHH
QFESHVDEGVRQAAPQVAVAA
HHHHHHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA