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The map label for this gene is phaB [H]

Identifier: 103487428

GI number: 103487428

Start: 2048964

End: 2049713

Strand: Direct

Name: phaB [H]

Synonym: Sala_1944

Alternate gene names: 103487428

Gene position: 2048964-2049713 (Clockwise)

Preceding gene: 103487427

Following gene: 103487432

Centisome position: 61.25

GC content: 72.4

Gene sequence:

>750_bases
ATGAGCAGGATCAGCGGCCGCCCGGCGATCAGCACCCAGCATGTCCATGGCCTGACGCTGCGCGTCGCGCGCTGGCGCCA
GGGTGCGCCGGGCACCCCCCTCCTGTTCCTCAACGGCATCGGCGCCGACATTGCTGCGGCGGCACCGCTGCTTGCGCAGA
TGCACGGGCGCGAGGCGTGGACGTTGGACATGCCGGGGGTCGGCGGCTCGCCCGATGCGCTGCTCCCCTATGCCGCGCCG
ACGATGGCCGCGATCGTCATGGAAATCGCCCGACGGTTCGGTCACGAGTCGCTCGACGTCGCGGGCTTCAGCTGGGGCGG
CGCGCTGGCGCAGCAGGTCGCGATCCAGTTTCCGGGGCGCGTCCGCCGACTGGTGCTGATGGCGACGACGCCGAGCGTCG
GCGCGCCGGGGATCGGCTGGGCCGCGCTGCTCGACGACGACATGCTGGCGAGCGGACTCAGGCGGCCGACCGCGACGCCG
CTTGGCCTTGCCTATCAGTCGCTGGCGATGGCGGGGTGGACGAGCGCGGCGATGCTGCCACTGTTGAAGGACGTGCCCGT
GCTGGTGCTGATGGGCGAGCGCGACGGCGTGGTCCCCGCCTGCCACGGACAGACGATCGCCGAGCTGATCGACGGCGCGG
TGCTGGAGGTGGTGCCGGGCTCGCACCTTTTTCCCTTCACCCACGCCGGGACGGTCGCGGCGCGGATCAGCGCCTTTCTG
GAGCGGCCGCCGCTGCAAGCCGCCGCGTGA

Upstream 100 bases:

>100_bases
CGATTGCCTGCGCTACGAACAGGCGGAGGCGACCGCAAAGGATCGTGGCTGACGCCGATGGGTCTTTCGCGCGTGATCGC
GATATGCGACAGCGTCGGAC

Downstream 100 bases:

>100_bases
GGAAATCGGCAATCGCCGTGGCAACCTGATCGGGCGCTTCTTCCATCGGCAAATGGCCGATTCCAGGGAGCAATACGACC
TCCGACCCCGCGATCCGCTC

Product: alpha/beta hydrolase fold

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSRISGRPAISTQHVHGLTLRVARWRQGAPGTPLLFLNGIGADIAAAAPLLAQMHGREAWTLDMPGVGGSPDALLPYAAP
TMAAIVMEIARRFGHESLDVAGFSWGGALAQQVAIQFPGRVRRLVLMATTPSVGAPGIGWAALLDDDMLASGLRRPTATP
LGLAYQSLAMAGWTSAAMLPLLKDVPVLVLMGERDGVVPACHGQTIAELIDGAVLEVVPGSHLFPFTHAGTVAARISAFL
ERPPLQAAA

Sequences:

>Translated_249_residues
MSRISGRPAISTQHVHGLTLRVARWRQGAPGTPLLFLNGIGADIAAAAPLLAQMHGREAWTLDMPGVGGSPDALLPYAAP
TMAAIVMEIARRFGHESLDVAGFSWGGALAQQVAIQFPGRVRRLVLMATTPSVGAPGIGWAALLDDDMLASGLRRPTATP
LGLAYQSLAMAGWTSAAMLPLLKDVPVLVLMGERDGVVPACHGQTIAELIDGAVLEVVPGSHLFPFTHAGTVAARISAFL
ERPPLQAAA
>Mature_248_residues
SRISGRPAISTQHVHGLTLRVARWRQGAPGTPLLFLNGIGADIAAAAPLLAQMHGREAWTLDMPGVGGSPDALLPYAAPT
MAAIVMEIARRFGHESLDVAGFSWGGALAQQVAIQFPGRVRRLVLMATTPSVGAPGIGWAALLDDDMLASGLRRPTATPL
GLAYQSLAMAGWTSAAMLPLLKDVPVLVLMGERDGVVPACHGQTIAELIDGAVLEVVPGSHLFPFTHAGTVAARISAFLE
RPPLQAAA

Specific function: PHA depolymerase is in fact a lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011942 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 25924; Mature: 25792

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISGRPAISTQHVHGLTLRVARWRQGAPGTPLLFLNGIGADIAAAAPLLAQMHGREAW
CCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEE
TLDMPGVGGSPDALLPYAAPTMAAIVMEIARRFGHESLDVAGFSWGGALAQQVAIQFPGR
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCC
VRRLVLMATTPSVGAPGIGWAALLDDDMLASGLRRPTATPLGLAYQSLAMAGWTSAAMLP
CEEEEEEEECCCCCCCCCCHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHH
LLKDVPVLVLMGERDGVVPACHGQTIAELIDGAVLEVVPGSHLFPFTHAGTVAARISAFL
HHCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
ERPPLQAAA
HCCCCCCCC
>Mature Secondary Structure 
SRISGRPAISTQHVHGLTLRVARWRQGAPGTPLLFLNGIGADIAAAAPLLAQMHGREAW
CCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEE
TLDMPGVGGSPDALLPYAAPTMAAIVMEIARRFGHESLDVAGFSWGGALAQQVAIQFPGR
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCC
VRRLVLMATTPSVGAPGIGWAALLDDDMLASGLRRPTATPLGLAYQSLAMAGWTSAAMLP
CEEEEEEEECCCCCCCCCCHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHH
LLKDVPVLVLMGERDGVVPACHGQTIAELIDGAVLEVVPGSHLFPFTHAGTVAARISAFL
HHCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
ERPPLQAAA
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]