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The map label for this gene is garR [C]

Identifier: 103487327

GI number: 103487327

Start: 1937250

End: 1938128

Strand: Direct

Name: garR [C]

Synonym: Sala_1843

Alternate gene names: 103487327

Gene position: 1937250-1938128 (Clockwise)

Preceding gene: 103487317

Following gene: 103487328

Centisome position: 57.91

GC content: 68.15

Gene sequence:

>879_bases
ATGAGCGAACAGAAATTGCGCATCAGCTTCATCGGCACCGGCGTCATGGGCGGGCCGATGGCGGGCCACCTCGTCCGGGC
GGGTCATCACCTCACCGTCTATAACCGCACGCGCGCCAGGGCCGATGCGTGGGCGGCGCGGCATGGCGGCGCGGTCGCCG
TCAGTCCGGCCGACGCCGCGCGCGACGCCGACATCGTCATCGCCTGTGTCGGCAACGACGATGATCTGGCGCAGGTGACA
TTGGGCCGCGACGGCGCGTTCCGGGCGATGCGCAAGGGTTCGCTGTTCATCGACCACACCACCGTCTCCGCGCGAATCGC
GCGCCAACTGTCGGTCGAGGCCGCAAGTCTCGGCCTCCTCTGTCTCGACGCCCCCGTTTCGGGCGGCGAGGCGGGCGCGC
AGAACGGCACGCTGTCGATCATGTGCGGGGGGACGCAAACCGCCTTCGCGGCGGCCGAGCCGGTGATGCAGGCCTATGCC
GCGCGAATGGTCCACATCGGCGGCCCCGGCGCGGGGCAGACGACCAAGATGGTCAACCAGATCGCGATCGCGGGCGTGCT
GCAAGGATTGTCCGAGGCGTTGCGCTTTGCGCAGGCGTCGAAGCTCGATACCGACAAGGTGTTCGAGGCGGTGTCGGGCG
GCGCGGCGGCAAGCTGGCAAATGCTCAACCGCTGGAGCACGATGGCGAAGGACGAATTCGACTTCGGTTTCGCGGTCGAC
TGGATGCGCAAGGATCTGGGCCTCGCGATCGACGAAGCGCGCGTCAACGGCGCGACCCTGCCGGTCGCCAGCCTCGTCGA
TCAATTCTACGCCGATGTGCAAAAGGTCGGCGGCGGGCGCAAGGACACGAGCTCGCTCGTGACGCGGCTGCCGAAATGA

Upstream 100 bases:

>100_bases
CAAGGTCGAGAACCCCCACCCCAACCCCTCCCCTGAAGGGGAGGGGCTTGATTGCGCTGCTCGCTCTGGCACCCCATCAA
AACAATGCTATGGACCCAGC

Downstream 100 bases:

>100_bases
AGCGCCTTATCCTGACCGCGCTGGCCCTCACCCTCGCCGCCCCCGCGCACGCCGATACGCTGGTCGACAATGTCAACGGC
ATCACGCTCGACAGGGACGG

Product: 6-phosphogluconate dehydrogenase, NAD-binding

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSEQKLRISFIGTGVMGGPMAGHLVRAGHHLTVYNRTRARADAWAARHGGAVAVSPADAARDADIVIACVGNDDDLAQVT
LGRDGAFRAMRKGSLFIDHTTVSARIARQLSVEAASLGLLCLDAPVSGGEAGAQNGTLSIMCGGTQTAFAAAEPVMQAYA
ARMVHIGGPGAGQTTKMVNQIAIAGVLQGLSEALRFAQASKLDTDKVFEAVSGGAAASWQMLNRWSTMAKDEFDFGFAVD
WMRKDLGLAIDEARVNGATLPVASLVDQFYADVQKVGGGRKDTSSLVTRLPK

Sequences:

>Translated_292_residues
MSEQKLRISFIGTGVMGGPMAGHLVRAGHHLTVYNRTRARADAWAARHGGAVAVSPADAARDADIVIACVGNDDDLAQVT
LGRDGAFRAMRKGSLFIDHTTVSARIARQLSVEAASLGLLCLDAPVSGGEAGAQNGTLSIMCGGTQTAFAAAEPVMQAYA
ARMVHIGGPGAGQTTKMVNQIAIAGVLQGLSEALRFAQASKLDTDKVFEAVSGGAAASWQMLNRWSTMAKDEFDFGFAVD
WMRKDLGLAIDEARVNGATLPVASLVDQFYADVQKVGGGRKDTSSLVTRLPK
>Mature_291_residues
SEQKLRISFIGTGVMGGPMAGHLVRAGHHLTVYNRTRARADAWAARHGGAVAVSPADAARDADIVIACVGNDDDLAQVTL
GRDGAFRAMRKGSLFIDHTTVSARIARQLSVEAASLGLLCLDAPVSGGEAGAQNGTLSIMCGGTQTAFAAAEPVMQAYAA
RMVHIGGPGAGQTTKMVNQIAIAGVLQGLSEALRFAQASKLDTDKVFEAVSGGAAASWQMLNRWSTMAKDEFDFGFAVDW
MRKDLGLAIDEARVNGATLPVASLVDQFYADVQKVGGGRKDTSSLVTRLPK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=296, Percent_Identity=30.4054054054054, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=24.6428571428571, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=35.2941176470588, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=35.6643356643357, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1790315, Length=290, Percent_Identity=31.3793103448276, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1789092, Length=288, Percent_Identity=29.8611111111111, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=29.368029739777, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24655230, Length=295, Percent_Identity=30.8474576271186, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI19922568, Length=295, Percent_Identity=30.8474576271186, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI28574115, Length=289, Percent_Identity=22.8373702422145, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30533; Mature: 30402

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKLRISFIGTGVMGGPMAGHLVRAGHHLTVYNRTRARADAWAARHGGAVAVSPADAA
CCCCEEEEEEEECCCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHCCCEEEECCCCCC
RDADIVIACVGNDDDLAQVTLGRDGAFRAMRKGSLFIDHTTVSARIARQLSVEAASLGLL
CCCCEEEEEECCCCCEEEEEECCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCEE
CLDAPVSGGEAGAQNGTLSIMCGGTQTAFAAAEPVMQAYAARMVHIGGPGAGQTTKMVNQ
EEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHH
IAIAGVLQGLSEALRFAQASKLDTDKVFEAVSGGAAASWQMLNRWSTMAKDEFDFGFAVD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
WMRKDLGLAIDEARVNGATLPVASLVDQFYADVQKVGGGRKDTSSLVTRLPK
HHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SEQKLRISFIGTGVMGGPMAGHLVRAGHHLTVYNRTRARADAWAARHGGAVAVSPADAA
CCCEEEEEEEECCCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHCCCEEEECCCCCC
RDADIVIACVGNDDDLAQVTLGRDGAFRAMRKGSLFIDHTTVSARIARQLSVEAASLGLL
CCCCEEEEEECCCCCEEEEEECCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCEE
CLDAPVSGGEAGAQNGTLSIMCGGTQTAFAAAEPVMQAYAARMVHIGGPGAGQTTKMVNQ
EEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHH
IAIAGVLQGLSEALRFAQASKLDTDKVFEAVSGGAAASWQMLNRWSTMAKDEFDFGFAVD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
WMRKDLGLAIDEARVNGATLPVASLVDQFYADVQKVGGGRKDTSSLVTRLPK
HHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA