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The map label for this gene is nrdF [H]

Identifier: 103487260

GI number: 103487260

Start: 1873486

End: 1874550

Strand: Direct

Name: nrdF [H]

Synonym: Sala_1775

Alternate gene names: 103487260

Gene position: 1873486-1874550 (Clockwise)

Preceding gene: 103487259

Following gene: 103487261

Centisome position: 56.01

GC content: 60.09

Gene sequence:

>1065_bases
ATGCCCCTTCTAGAAGCCCGCAAGACCTACAAGCCCTTCGAATATCCCTGGGCGTTCGATTTCTGGAAGCGCCAGCAGCA
GATCCACTGGGTGCCCGAGGAAGTGCCGCTGGGCGAGGATTGCCGCGACTGGGCGCAAAAGATCAGCGACCATGAACGCA
ACCTGCTCACCCAGATTTTCCGCTTCTTCACCCAGGCCGATATCGAGGTTCAAGATTGCTACCACGAAAAATACGGCCGC
GTGTTCAAGCCGACCGAAATCAAGATGATGCTGACCGCGTTCAGCAACATGGAGACGGTGCATATCGCGGCGTACAGCCA
CCTGCTCGACACCATCGGCATGCCCGAAAGCGAATATGGCATGTTCCTCGAATATGACGAGATGCGCGCCAAGCATGATT
ATATGCAGCAGTTCGGCGTCGAAACCGACGAGGACATCGCGCGCACCCTCGCGATGTTCGGCGGCTTTACCGAGGGGCTT
CAGCTCTTCGCCAGCTTCGCGATGCTGATGAACTTCCCGCGCTTCAACAAGATGAAGGGCATGGGCCAGATCGTCAGCTG
GTCGATCCGCGACGAAAGCCTGCATTGCGAAGGCATCATCAAGCTGTTCCACGCCTTCACCAAGGAACGCGGCTGCCTGA
CCAAGGCGGTGAAGGAAGACATCATCGACACCTGCCAGAAAACGGTGCGGCTCGAGGATGCGTTCATCGACCTCGCGTTC
GAACAGGGCCCCGTCCCCGGCATGACGCCCAAGGAGATCAAGCGCTACATCCGCTACATCGCCGACTGGCGCCTGGGGCA
ATTGGGGTTCCAGCCGATCTACATGATCGACGAACACCCCCTCCCCTGGCTCGCCCCGCTGCTGAACGGCGTCGAGCACG
CCAACTTCTTCGAAACGCGCGCGACCGAGTACAGCAAGGGCGCGACGCGCGGCGACTGGAACACGGTGTGGACCAGCTTC
GACAACCGCAAGAAAGCCAAGGCGAACGACGACGGCGCGACGACTCCCGCCGCCGAAAGTGACGGCGAGGATATGTTCGC
CAAGGCAGGGATCGCCGCCGAATGA

Upstream 100 bases:

>100_bases
CAACCGCCAACCACCCTCACCTTCCCAAGGCTGACGCCTTGGGCCCCTTCCTCTCCCGGTGGGAGAGGAGTAAAGACATG
CCAGTCCGGAGACCGATGAG

Downstream 100 bases:

>100_bases
GACGCTGAAAACCCCTCCCGCTTGCGGGAGGGGCAGCGAGACTTGCGGGCTTGCCCGCTAGTCGCAGCGGGGTGGGCAGG
AAGGAAAGCACATTGCCCAA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MPLLEARKTYKPFEYPWAFDFWKRQQQIHWVPEEVPLGEDCRDWAQKISDHERNLLTQIFRFFTQADIEVQDCYHEKYGR
VFKPTEIKMMLTAFSNMETVHIAAYSHLLDTIGMPESEYGMFLEYDEMRAKHDYMQQFGVETDEDIARTLAMFGGFTEGL
QLFASFAMLMNFPRFNKMKGMGQIVSWSIRDESLHCEGIIKLFHAFTKERGCLTKAVKEDIIDTCQKTVRLEDAFIDLAF
EQGPVPGMTPKEIKRYIRYIADWRLGQLGFQPIYMIDEHPLPWLAPLLNGVEHANFFETRATEYSKGATRGDWNTVWTSF
DNRKKAKANDDGATTPAAESDGEDMFAKAGIAAE

Sequences:

>Translated_354_residues
MPLLEARKTYKPFEYPWAFDFWKRQQQIHWVPEEVPLGEDCRDWAQKISDHERNLLTQIFRFFTQADIEVQDCYHEKYGR
VFKPTEIKMMLTAFSNMETVHIAAYSHLLDTIGMPESEYGMFLEYDEMRAKHDYMQQFGVETDEDIARTLAMFGGFTEGL
QLFASFAMLMNFPRFNKMKGMGQIVSWSIRDESLHCEGIIKLFHAFTKERGCLTKAVKEDIIDTCQKTVRLEDAFIDLAF
EQGPVPGMTPKEIKRYIRYIADWRLGQLGFQPIYMIDEHPLPWLAPLLNGVEHANFFETRATEYSKGATRGDWNTVWTSF
DNRKKAKANDDGATTPAAESDGEDMFAKAGIAAE
>Mature_353_residues
PLLEARKTYKPFEYPWAFDFWKRQQQIHWVPEEVPLGEDCRDWAQKISDHERNLLTQIFRFFTQADIEVQDCYHEKYGRV
FKPTEIKMMLTAFSNMETVHIAAYSHLLDTIGMPESEYGMFLEYDEMRAKHDYMQQFGVETDEDIARTLAMFGGFTEGLQ
LFASFAMLMNFPRFNKMKGMGQIVSWSIRDESLHCEGIIKLFHAFTKERGCLTKAVKEDIIDTCQKTVRLEDAFIDLAFE
QGPVPGMTPKEIKRYIRYIADWRLGQLGFQPIYMIDEHPLPWLAPLLNGVEHANFFETRATEYSKGATRGDWNTVWTSFD
NRKKAKANDDGATTPAAESDGEDMFAKAGIAAE

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI289177074, Length=306, Percent_Identity=26.797385620915, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI42544136, Length=321, Percent_Identity=27.1028037383178, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI260064013, Length=304, Percent_Identity=26.9736842105263, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI4557845, Length=304, Percent_Identity=26.9736842105263, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI289177076, Length=285, Percent_Identity=26.3157894736842, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1788567, Length=296, Percent_Identity=19.9324324324324, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789031, Length=312, Percent_Identity=23.0769230769231, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17551968, Length=310, Percent_Identity=25.4838709677419, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322434, Length=319, Percent_Identity=25.3918495297806, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI18079279, Length=313, Percent_Identity=26.1980830670927, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 41068; Mature: 40937

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLEARKTYKPFEYPWAFDFWKRQQQIHWVPEEVPLGEDCRDWAQKISDHERNLLTQIF
CCCCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFFTQADIEVQDCYHEKYGRVFKPTEIKMMLTAFSNMETVHIAAYSHLLDTIGMPESEYG
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCC
MFLEYDEMRAKHDYMQQFGVETDEDIARTLAMFGGFTEGLQLFASFAMLMNFPRFNKMKG
CEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCC
MGQIVSWSIRDESLHCEGIIKLFHAFTKERGCLTKAVKEDIIDTCQKTVRLEDAFIDLAF
CCHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGPVPGMTPKEIKRYIRYIADWRLGQLGFQPIYMIDEHPLPWLAPLLNGVEHANFFETR
HCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ATEYSKGATRGDWNTVWTSFDNRKKAKANDDGATTPAAESDGEDMFAKAGIAAE
HHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure 
PLLEARKTYKPFEYPWAFDFWKRQQQIHWVPEEVPLGEDCRDWAQKISDHERNLLTQIF
CCCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFFTQADIEVQDCYHEKYGRVFKPTEIKMMLTAFSNMETVHIAAYSHLLDTIGMPESEYG
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCC
MFLEYDEMRAKHDYMQQFGVETDEDIARTLAMFGGFTEGLQLFASFAMLMNFPRFNKMKG
CEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCC
MGQIVSWSIRDESLHCEGIIKLFHAFTKERGCLTKAVKEDIIDTCQKTVRLEDAFIDLAF
CCHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGPVPGMTPKEIKRYIRYIADWRLGQLGFQPIYMIDEHPLPWLAPLLNGVEHANFFETR
HCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ATEYSKGATRGDWNTVWTSFDNRKKAKANDDGATTPAAESDGEDMFAKAGIAAE
HHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]