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The map label for this gene is purN [H]

Identifier: 103487243

GI number: 103487243

Start: 1853279

End: 1854226

Strand: Direct

Name: purN [H]

Synonym: Sala_1758

Alternate gene names: 103487243

Gene position: 1853279-1854226 (Clockwise)

Preceding gene: 103487242

Following gene: 103487247

Centisome position: 55.4

GC content: 65.93

Gene sequence:

>948_bases
ATGAAAGCCAAGGTCGCCGTCCTGATCTCCGGCGCCGGTACCAATATGGCGGCGCTCCTTTACGCGGCGAAGGCCGAAGC
CTGCCCCTATGAACTGGTGCTCGTCGCGAGCAACGACCCCGGCGCGCCGGGGCTGAAGCTCGCCGAGGCCGAGGGGGTCG
CGACCTGGGCGCATTCGCACAAGGGCCTGCCGCGCGATGCCTTCGACGCGCTGGTCGACGAACAGCTGCGTGCCGCAGGC
GCCGACTATGTCGCGCTTGCGGGCTATATGCGCATCCTGTCGGACGCTTTCGTCGAACGCTGGGTGGGACGGATGCTCAA
TATCCACCCCAGCCTGCTGCCGAAATACAAGGGGCTGAACACGCACGCGCAGGCGATCGCGAATGACGACAAGTTCGGCG
GGTGCAGCGTCCATATCGTCACCCCCGCACTCGACGACGGCCCCGTGCTCGCACAAACGCCGGTCGCGATCGTTCCCGGC
GACACGCCCGAAACGCTCGCCGCGCGCGTCCGATTCGCCGAGCATCAGCTTTATCCCGCCACGCTCGCGGCCTATGTCGC
GCGCGAACGCTCGCCCGATTATCTGCGCGGCCGCGTGCGCGACCTCGCGCTGGCGCTGCCCGAAAGCGACGAAGTGCTGT
CGCATGGGATGCCCTGTTTCGGGATCGTCAAGGGCAAGAAGTTCGCCTATTTCACCGAAGATCATCATGGCGACGGCAAG
ATCGCCCTGCTCGTCAAGGTCAGCGGCGCCGACGAACAGGCGCAGCTCATCGAACTCGATCCCGACCGCTATTACCGCCC
CGCCTATTTCGGCGACGGCTGGATCGGCATCCGGCTCGACCTCGGCGATACCGACTGGGACGCGATCGCTGAATGGCTGC
GCAAGAGCTGGCTGACGGTTGCACCGAAAAAGCTGGCGGGGCTGATGGCGGCGGCGGAGGATTTCTGA

Upstream 100 bases:

>100_bases
GGTACGCGCAGATCTGTGCGGCCTTTCAAGATCAGCCGGTCCAGCCTGCGCCCCTCCACCAGCTTCGCTGGTCCCCATCC
CCGTTCCGGGAAGGATCGGC

Downstream 100 bases:

>100_bases
TGAAAAAGGCCGCCGGATCGCTCCGGCGGCCTTTTCGTTCCGGTTCGGCCGTCTGGGTCAGCCGACGATTTCTTCGGGCT
TGAAGAAATAGGCGATTTCG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKAKVAVLISGAGTNMAALLYAAKAEACPYELVLVASNDPGAPGLKLAEAEGVATWAHSHKGLPRDAFDALVDEQLRAAG
ADYVALAGYMRILSDAFVERWVGRMLNIHPSLLPKYKGLNTHAQAIANDDKFGGCSVHIVTPALDDGPVLAQTPVAIVPG
DTPETLAARVRFAEHQLYPATLAAYVARERSPDYLRGRVRDLALALPESDEVLSHGMPCFGIVKGKKFAYFTEDHHGDGK
IALLVKVSGADEQAQLIELDPDRYYRPAYFGDGWIGIRLDLGDTDWDAIAEWLRKSWLTVAPKKLAGLMAAAEDF

Sequences:

>Translated_315_residues
MKAKVAVLISGAGTNMAALLYAAKAEACPYELVLVASNDPGAPGLKLAEAEGVATWAHSHKGLPRDAFDALVDEQLRAAG
ADYVALAGYMRILSDAFVERWVGRMLNIHPSLLPKYKGLNTHAQAIANDDKFGGCSVHIVTPALDDGPVLAQTPVAIVPG
DTPETLAARVRFAEHQLYPATLAAYVARERSPDYLRGRVRDLALALPESDEVLSHGMPCFGIVKGKKFAYFTEDHHGDGK
IALLVKVSGADEQAQLIELDPDRYYRPAYFGDGWIGIRLDLGDTDWDAIAEWLRKSWLTVAPKKLAGLMAAAEDF
>Mature_315_residues
MKAKVAVLISGAGTNMAALLYAAKAEACPYELVLVASNDPGAPGLKLAEAEGVATWAHSHKGLPRDAFDALVDEQLRAAG
ADYVALAGYMRILSDAFVERWVGRMLNIHPSLLPKYKGLNTHAQAIANDDKFGGCSVHIVTPALDDGPVLAQTPVAIVPG
DTPETLAARVRFAEHQLYPATLAAYVARERSPDYLRGRVRDLALALPESDEVLSHGMPCFGIVKGKKFAYFTEDHHGDGK
IALLVKVSGADEQAQLIELDPDRYYRPAYFGDGWIGIRLDLGDTDWDAIAEWLRKSWLTVAPKKLAGLMAAAEDF

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=182, Percent_Identity=44.5054945054945, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI209869995, Length=182, Percent_Identity=44.5054945054945, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI209869993, Length=182, Percent_Identity=44.5054945054945, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1788846, Length=181, Percent_Identity=43.646408839779, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1787483, Length=187, Percent_Identity=27.807486631016, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=37.4331550802139, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=43.0851063829787, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 34049; Mature: 34049

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKVAVLISGAGTNMAALLYAAKAEACPYELVLVASNDPGAPGLKLAEAEGVATWAHSH
CCCEEEEEEECCCCCHHHHEEHHHCCCCCEEEEEEECCCCCCCCCEEECCCCEEEHHHHC
KGLPRDAFDALVDEQLRAAGADYVALAGYMRILSDAFVERWVGRMLNIHPSLLPKYKGLN
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC
THAQAIANDDKFGGCSVHIVTPALDDGPVLAQTPVAIVPGDTPETLAARVRFAEHQLYPA
HHHHHHCCCCCCCCEEEEEEECCCCCCCEEECCCEEEECCCCHHHHHHHHHHHHHCCCHH
TLAAYVARERSPDYLRGRVRDLALALPESDEVLSHGMPCFGIVKGKKFAYFTEDHHGDGK
HHHHHHHHCCCCHHHHHHHHHHEEECCCCHHHHHCCCCEEEEEECCEEEEEECCCCCCCE
IALLVKVSGADEQAQLIELDPDRYYRPAYFGDGWIGIRLDLGDTDWDAIAEWLRKSWLTV
EEEEEEECCCCCCCEEEEECCCCCCCCCEECCCEEEEEEECCCCCHHHHHHHHHHHCCCC
APKKLAGLMAAAEDF
CHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAKVAVLISGAGTNMAALLYAAKAEACPYELVLVASNDPGAPGLKLAEAEGVATWAHSH
CCCEEEEEEECCCCCHHHHEEHHHCCCCCEEEEEEECCCCCCCCCEEECCCCEEEHHHHC
KGLPRDAFDALVDEQLRAAGADYVALAGYMRILSDAFVERWVGRMLNIHPSLLPKYKGLN
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC
THAQAIANDDKFGGCSVHIVTPALDDGPVLAQTPVAIVPGDTPETLAARVRFAEHQLYPA
HHHHHHCCCCCCCCEEEEEEECCCCCCCEEECCCEEEECCCCHHHHHHHHHHHHHCCCHH
TLAAYVARERSPDYLRGRVRDLALALPESDEVLSHGMPCFGIVKGKKFAYFTEDHHGDGK
HHHHHHHHCCCCHHHHHHHHHHEEECCCCHHHHHCCCCEEEEEECCEEEEEECCCCCCCE
IALLVKVSGADEQAQLIELDPDRYYRPAYFGDGWIGIRLDLGDTDWDAIAEWLRKSWLTV
EEEEEEECCCCCCCEEEEECCCCCCCCCEECCCEEEEEEECCCCCHHHHHHHHHHHCCCC
APKKLAGLMAAAEDF
CHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]