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The map label for this gene is tyrS [H]

Identifier: 103487134

GI number: 103487134

Start: 1731220

End: 1732488

Strand: Direct

Name: tyrS [H]

Synonym: Sala_1649

Alternate gene names: 103487134

Gene position: 1731220-1732488 (Clockwise)

Preceding gene: 103487129

Following gene: 103487135

Centisome position: 51.75

GC content: 62.81

Gene sequence:

>1269_bases
GTGTTGGGTTTGCTTCGACGTCATGCTAAGCGCCCGCCCGCTATGACCAACTATCAATCCGACCTGCTGCGCCTTCTCGA
CGAGCGAGGCTATATCCATCAGATGACCGACGCGGAAGGGCTCGATGCGCTCGCCGCGAAACAGATCGTCCCCGGATATA
TCGGCTTCGACGCGACCGCGCCCAGCCTGCACGTCGGCAGCCTCGTCCAGATCATGATGCTGCGCCGCCTGCAACAGACG
GGGCACAAGCCCATTGTGCTGATGGGCGGCGGGACGACGCGGATCGGCGACCCGACGGGGCGTGACGAAAGCCGCAAGAT
GCTGTCGGACGAGGTGATCGCCGCGAACATCGCGTCGATCTTCAGCATCTTTCGGCAGTTCCTGGTGTTCGGCGATGGTC
CGACCGACGCGGTGATGGTCGATAATCAGGACTGGCTCGGCAAGCTCGGCTATATCGAGCTGCTCCAAGAAGTCGGCAGG
CATTTCACGATCAATCGCATGTTGACCTTTGATTCGGTCAAGCTGCGGCTCGAGCGCGAACAGCCGATGACCTTCCTCGA
GTTCAATTACATGATCCTCCAGGGCTATGACTTTCGCCACCTGTCGCGCGAAATGGGTGTGCGGCTCCAGATGGGGGGAT
CGGACCAGTGGGGCAATATCGTCAACGGCATGGAACTGGGCCGCCGCATGGACGGCGCCGATCTCTATGGCCTGACGACT
CCGCTGCTCACCACCGCCGCGGGCGCCAAAATGGGCAAAACGGCTGCCGGCGCGGTCTGGCTTAATCCCGAACAACTGTC
CCATTTTGACTATTGGCAATATTGGCGCAACTGCGACGACCGCGATGTCGGCAAGTTCCTGAAGCTCTTCACCGACCTGC
CGCTCGCCGAGATTGCGCGCCTCGAAGCGCTCGAGGGCGCCGAGATCAACGAGGCGAAGAAGATTCTCGCCAACGAGGCG
ACCGCAATGTGCCGCGGCGTCGATGCAGCCAGCACCGCGGCCGAGACCGCCACGCAAACCTTTGAAAAGGGCCGGATTGG
CGGCGACCTGCCGCACGTCGCTGCGCCCGCCGACGGGATCGGCATCGTCGATGCCCTGCGCGAACTCGGCTTTGCTGCCT
CGAACAAGGAAGCGCGCCGCAAGCTCGAAGAGGGCGCGGTGAGGGTGGACGGCAGCGTCGTTCGCGACCCCAGCTATCGG
ATCGTCCCGGCGGGCGGCGACATTGCGATCAGCCTCGGCGCCAAAAAACATGGTCTCGTGACGCGGTAA

Upstream 100 bases:

>100_bases
GTGTAAAAGGCCCCTCCGCGGCAATGCAACATATAGGCCCAAAAGCTCATGGGCACGGTGTAAAAGGAAGAACGGCCCTT
CGACAAGCTCAGGGCGAACG

Downstream 100 bases:

>100_bases
GGCACAAACAGCATTTACCACTTCTTCAGCATTCCGGCGGAACGTCATGGCCAAATCAACAAGGTCCACGAAAATGCGCA
AGATTGACACGAGCAAGGCC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MLGLLRRHAKRPPAMTNYQSDLLRLLDERGYIHQMTDAEGLDALAAKQIVPGYIGFDATAPSLHVGSLVQIMMLRRLQQT
GHKPIVLMGGGTTRIGDPTGRDESRKMLSDEVIAANIASIFSIFRQFLVFGDGPTDAVMVDNQDWLGKLGYIELLQEVGR
HFTINRMLTFDSVKLRLEREQPMTFLEFNYMILQGYDFRHLSREMGVRLQMGGSDQWGNIVNGMELGRRMDGADLYGLTT
PLLTTAAGAKMGKTAAGAVWLNPEQLSHFDYWQYWRNCDDRDVGKFLKLFTDLPLAEIARLEALEGAEINEAKKILANEA
TAMCRGVDAASTAAETATQTFEKGRIGGDLPHVAAPADGIGIVDALRELGFAASNKEARRKLEEGAVRVDGSVVRDPSYR
IVPAGGDIAISLGAKKHGLVTR

Sequences:

>Translated_422_residues
MLGLLRRHAKRPPAMTNYQSDLLRLLDERGYIHQMTDAEGLDALAAKQIVPGYIGFDATAPSLHVGSLVQIMMLRRLQQT
GHKPIVLMGGGTTRIGDPTGRDESRKMLSDEVIAANIASIFSIFRQFLVFGDGPTDAVMVDNQDWLGKLGYIELLQEVGR
HFTINRMLTFDSVKLRLEREQPMTFLEFNYMILQGYDFRHLSREMGVRLQMGGSDQWGNIVNGMELGRRMDGADLYGLTT
PLLTTAAGAKMGKTAAGAVWLNPEQLSHFDYWQYWRNCDDRDVGKFLKLFTDLPLAEIARLEALEGAEINEAKKILANEA
TAMCRGVDAASTAAETATQTFEKGRIGGDLPHVAAPADGIGIVDALRELGFAASNKEARRKLEEGAVRVDGSVVRDPSYR
IVPAGGDIAISLGAKKHGLVTR
>Mature_422_residues
MLGLLRRHAKRPPAMTNYQSDLLRLLDERGYIHQMTDAEGLDALAAKQIVPGYIGFDATAPSLHVGSLVQIMMLRRLQQT
GHKPIVLMGGGTTRIGDPTGRDESRKMLSDEVIAANIASIFSIFRQFLVFGDGPTDAVMVDNQDWLGKLGYIELLQEVGR
HFTINRMLTFDSVKLRLEREQPMTFLEFNYMILQGYDFRHLSREMGVRLQMGGSDQWGNIVNGMELGRRMDGADLYGLTT
PLLTTAAGAKMGKTAAGAVWLNPEQLSHFDYWQYWRNCDDRDVGKFLKLFTDLPLAEIARLEALEGAEINEAKKILANEA
TAMCRGVDAASTAAETATQTFEKGRIGGDLPHVAAPADGIGIVDALRELGFAASNKEARRKLEEGAVRVDGSVVRDPSYR
IVPAGGDIAISLGAKKHGLVTR

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=374, Percent_Identity=34.4919786096257, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI1787925, Length=396, Percent_Identity=38.3838383838384, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI25145802, Length=343, Percent_Identity=30.3206997084548, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI25145805, Length=293, Percent_Identity=26.6211604095563, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6325160, Length=408, Percent_Identity=32.843137254902, Blast_Score=216, Evalue=6e-57,
Organism=Drosophila melanogaster, GI20130383, Length=421, Percent_Identity=35.8669833729216, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46436; Mature: 46436

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLLRRHAKRPPAMTNYQSDLLRLLDERGYIHQMTDAEGLDALAAKQIVPGYIGFDATA
CCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCC
PSLHVGSLVQIMMLRRLQQTGHKPIVLMGGGTTRIGDPTGRDESRKMLSDEVIAANIASI
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FSIFRQFLVFGDGPTDAVMVDNQDWLGKLGYIELLQEVGRHFTINRMLTFDSVKLRLERE
HHHHHHHHEECCCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEHHEEEECHHEEEEECC
QPMTFLEFNYMILQGYDFRHLSREMGVRLQMGGSDQWGNIVNGMELGRRMDGADLYGLTT
CCCEEEEEEEEEEECCCHHHHHHHHCEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHH
PLLTTAAGAKMGKTAAGAVWLNPEQLSHFDYWQYWRNCDDRDVGKFLKLFTDLPLAEIAR
HHHHHHCCCCCCCCCCCEEEECHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHH
LEALEGAEINEAKKILANEATAMCRGVDAASTAAETATQTFEKGRIGGDLPHVAAPADGI
HHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GIVDALRELGFAASNKEARRKLEEGAVRVDGSVVRDPSYRIVPAGGDIAISLGAKKHGLV
HHHHHHHHHCCCCCCHHHHHHHHHCCEEECCEEEECCCEEEEECCCCEEEEECCCCCCCC
TR
CC
>Mature Secondary Structure
MLGLLRRHAKRPPAMTNYQSDLLRLLDERGYIHQMTDAEGLDALAAKQIVPGYIGFDATA
CCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCC
PSLHVGSLVQIMMLRRLQQTGHKPIVLMGGGTTRIGDPTGRDESRKMLSDEVIAANIASI
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FSIFRQFLVFGDGPTDAVMVDNQDWLGKLGYIELLQEVGRHFTINRMLTFDSVKLRLERE
HHHHHHHHEECCCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEHHEEEECHHEEEEECC
QPMTFLEFNYMILQGYDFRHLSREMGVRLQMGGSDQWGNIVNGMELGRRMDGADLYGLTT
CCCEEEEEEEEEEECCCHHHHHHHHCEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHH
PLLTTAAGAKMGKTAAGAVWLNPEQLSHFDYWQYWRNCDDRDVGKFLKLFTDLPLAEIAR
HHHHHHCCCCCCCCCCCEEEECHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHH
LEALEGAEINEAKKILANEATAMCRGVDAASTAAETATQTFEKGRIGGDLPHVAAPADGI
HHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GIVDALRELGFAASNKEARRKLEEGAVRVDGSVVRDPSYRIVPAGGDIAISLGAKKHGLV
HHHHHHHHHCCCCCCHHHHHHHHHCCEEECCEEEECCCEEEEECCCCEEEEECCCCCCCC
TR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA