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The map label for this gene is fabI1 [H]

Identifier: 103486150

GI number: 103486150

Start: 668161

End: 668988

Strand: Direct

Name: fabI1 [H]

Synonym: Sala_0657

Alternate gene names: 103486150

Gene position: 668161-668988 (Clockwise)

Preceding gene: 103486149

Following gene: 103486151

Centisome position: 19.97

GC content: 61.96

Gene sequence:

>828_bases
ATGACGGGTCTGATGAAGGGCAAACGCGGGCTGATCATGGGGCTGGCGAACGACAAATCGCTCGCCTGGGGCATTGCGAA
GGCGCTGCACGCGCATGGCGCGGAGCTTGCGCTCTCCTATCAGGGCGAGGTGATGGCAAAGCGCGTCAAGCCGCTTGCCG
ACCAGCTTGGCTGCGATTTCCTGATCGATTGCGACGTGTCCGACATGGACAATCTCGACGCAGCGTTCGGGTCGCTCGCC
GCGCGCTGGCCGACGATCGATTTTGTGGTCCACGCGATCGGCTATACCAACAAGGAAGCGCTGCGGGGCCATTATGCCGA
TGTGACGCTCGACGATTTCCTGATGACGATGAATATCAGCGTTTACAGCTTCACCGCGGTCGCGAAGCGCGCGGCGGCGA
TGATGGCGCCCTTCGACCCTGAAACGGGCGCCGGCGGCGGCTCGCTGCTGACGCTCAGCTATTATGGCGCCGAAAAGGTC
ATCCCGCATTATAACGTCATGGGCGTCGCGAAGTCGGCGCTCGAAACCAGCGTCAAATATCTTGCCAATGATTACGGGCC
GCAGGGGGTGCGCGTGAACGCGATCTCGGCAGGGCCGATCAAGACGCTCGCAGCGTCGGGCATCGGCGATTTCCGCCTGA
TCCTGAAATGGAACGAATATAACGCGCCGCTGCGCCGCAACGTCACGATCGACGATGTGGGCGGCTCGGCGCTCTATCTC
CTGAGCGATCTGGCGAGCGGCGTGACAGGCGAAACGCACCACGTCGATGCGGGTTATCACACCATCGGCATGAAGCAGGA
AGATGCGCCGGACATCGCGCTTGCGTGA

Upstream 100 bases:

>100_bases
TCAACGCCGCGCTCGTCGAGGTTGAGGATTTGGGCCACGACGCTATGGGGCGCGTGGACGACGTGACAGTCGACGATAAA
TAAGGCCGGAGAAAACGGAA

Downstream 100 bases:

>100_bases
TGGTTGATCTGACCATCGATCATGTTCAGCTTGCCATCCCCATCGGCGGTGAAGATGCGGCGCGGAGTTTTTATGGCGTA
TTGCTGGGCTTGAGTGAATT

Product: enoyl-acyl-carrier-protein reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTGLMKGKRGLIMGLANDKSLAWGIAKALHAHGAELALSYQGEVMAKRVKPLADQLGCDFLIDCDVSDMDNLDAAFGSLA
ARWPTIDFVVHAIGYTNKEALRGHYADVTLDDFLMTMNISVYSFTAVAKRAAAMMAPFDPETGAGGGSLLTLSYYGAEKV
IPHYNVMGVAKSALETSVKYLANDYGPQGVRVNAISAGPIKTLAASGIGDFRLILKWNEYNAPLRRNVTIDDVGGSALYL
LSDLASGVTGETHHVDAGYHTIGMKQEDAPDIALA

Sequences:

>Translated_275_residues
MTGLMKGKRGLIMGLANDKSLAWGIAKALHAHGAELALSYQGEVMAKRVKPLADQLGCDFLIDCDVSDMDNLDAAFGSLA
ARWPTIDFVVHAIGYTNKEALRGHYADVTLDDFLMTMNISVYSFTAVAKRAAAMMAPFDPETGAGGGSLLTLSYYGAEKV
IPHYNVMGVAKSALETSVKYLANDYGPQGVRVNAISAGPIKTLAASGIGDFRLILKWNEYNAPLRRNVTIDDVGGSALYL
LSDLASGVTGETHHVDAGYHTIGMKQEDAPDIALA
>Mature_274_residues
TGLMKGKRGLIMGLANDKSLAWGIAKALHAHGAELALSYQGEVMAKRVKPLADQLGCDFLIDCDVSDMDNLDAAFGSLAA
RWPTIDFVVHAIGYTNKEALRGHYADVTLDDFLMTMNISVYSFTAVAKRAAAMMAPFDPETGAGGGSLLTLSYYGAEKVI
PHYNVMGVAKSALETSVKYLANDYGPQGVRVNAISAGPIKTLAASGIGDFRLILKWNEYNAPLRRNVTIDDVGGSALYLL
SDLASGVTGETHHVDAGYHTIGMKQEDAPDIALA

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=265, Percent_Identity=49.4339622641509, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI87082100, Length=269, Percent_Identity=27.5092936802974, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29289; Mature: 29158

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLMKGKRGLIMGLANDKSLAWGIAKALHAHGAELALSYQGEVMAKRVKPLADQLGCDF
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCE
LIDCDVSDMDNLDAAFGSLAARWPTIDFVVHAIGYTNKEALRGHYADVTLDDFLMTMNIS
EEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCCCCCHHHEEEEEECH
VYSFTAVAKRAAAMMAPFDPETGAGGGSLLTLSYYGAEKVIPHYNVMGVAKSALETSVKY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
LANDYGPQGVRVNAISAGPIKTLAASGIGDFRLILKWNEYNAPLRRNVTIDDVGGSALYL
HHHCCCCCCEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCEECCCEEECCCCHHHHH
LSDLASGVTGETHHVDAGYHTIGMKQEDAPDIALA
HHHHHCCCCCCCEEECCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TGLMKGKRGLIMGLANDKSLAWGIAKALHAHGAELALSYQGEVMAKRVKPLADQLGCDF
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCE
LIDCDVSDMDNLDAAFGSLAARWPTIDFVVHAIGYTNKEALRGHYADVTLDDFLMTMNIS
EEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCCCCCHHHEEEEEECH
VYSFTAVAKRAAAMMAPFDPETGAGGGSLLTLSYYGAEKVIPHYNVMGVAKSALETSVKY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
LANDYGPQGVRVNAISAGPIKTLAASGIGDFRLILKWNEYNAPLRRNVTIDDVGGSALYL
HHHCCCCCCEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCEECCCEEECCCCHHHHH
LSDLASGVTGETHHVDAGYHTIGMKQEDAPDIALA
HHHHHCCCCCCCEEECCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]