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The map label for this gene is nylB [H]

Identifier: 103486145

GI number: 103486145

Start: 663481

End: 664632

Strand: Direct

Name: nylB [H]

Synonym: Sala_0652

Alternate gene names: 103486145

Gene position: 663481-664632 (Clockwise)

Preceding gene: 103486143

Following gene: 103486148

Centisome position: 19.83

GC content: 69.53

Gene sequence:

>1152_bases
ATGAAAAAATGGCTGCTGGCAAGTGCCGCCCTCGCGATGCTCGGCGCCTGGTCGCTGACACAGGCGGCGGGGCAGGGCAG
CCTCGCGCCCGCGGCGCCCGCGCCCAGGTTCGGCCTGTCGCTCGACGACGTGGCCGCGGCCGACGCTTCCTCCGCGCTCC
CCGCCTTGCCCGCGGCGCTCCGCGCGCGCGCCGACGCGCTGTTCGCCGATGCCGAGGCGGTGGGGCAGACGCGCGCGCTG
CTCGTGCTCCGCGATGGCGAGCCGATCTATGAACGCTATGCCGCGGGGTTCGGCCCGGACAGCAAGCTGATCAGCTGGTC
GATGGCGAAAAGCATCACCGCGGTGCTCACCGGCTTTCTGGTGGCCGACGGGCAATTGTCGCTCGACGCTCCGGCACCCG
TCGCGGCGTGGCAGCGCAGCGGCGATCCGCGCGGCGCGATCACGCTGCGCCACCTGCTCCACATGTCATCGGGGCTCGAA
CATGTCGAAAATGGCGATCCCGTGTGGGACGGCGACACGGTCGCGATGCTGTTCGGCGGCGGCGCGGCCGACATGGCGGG
CTTTGCCGAGGCCAAGCCCGCGGCGGCGCAGCCCGGCGAGGTGTTCAATTACAGCTCGGCGACGAGCATCATCCTGTCGG
ATATCATCGCCGACACGCTGACGCCGTCGGAAAGCCCCGACGCGCGGCGCGACGCGATGCGCGACTTTATCGCCGGGCGG
CTGATCGAACCGCTCGGCATGACCAGCCTGACGCCCGAGTTCGATGCGAAAGGCACGATGATCGGCGGGTCGATCATGCA
CGCAACCGCGCGCGACTATGCCCGCTTCGGCGAGTTTCTGCGCAACGACGGTGTCGTGAACGGCCAGCGGCTGCTGCCCG
AAAGCTGGATCCGTTTCATGCTGACGCCGTCGACGCGCGATGCGGGTTATGGTGGGCATATCTGGCTCAACCGCCGCCGC
CCGCCGGGGGCGCAGCCCGCGCTCTGGCCCGATCGCGGCCCGAACGACCTGTTCGCCGCCATCGGCCATCAGGGGCAATA
TATCATCGTCTCACCGTCGCAGCGCGTGACGATTGTGCGGCTGGGCGTGACCAGGGACGATCAGTTTCCCGCGCTGCGAC
GGCACCTGGCGGATCTGACCGCGGCCTTATAG

Upstream 100 bases:

>100_bases
GCGCGTCGAAACCGGGGCGGGCGGCGGTGGCGGGGCAGGCGTCGGCGTCGCACCCGTTGATGGGTCGGTGGAGTTGGTGT
TATTCACCCGGTCCATGATG

Downstream 100 bases:

>100_bases
CAGGAAATCGCCTACCAGCGTCGTGACGCAGCGGCCCGTCAGTTCGACCATGTCGCCGTCGAGGCGGCAGCCGAGGTGGC
CGCCACGCGCGCTCGCCTGA

Product: beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKKWLLASAALAMLGAWSLTQAAGQGSLAPAAPAPRFGLSLDDVAAADASSALPALPAALRARADALFADAEAVGQTRAL
LVLRDGEPIYERYAAGFGPDSKLISWSMAKSITAVLTGFLVADGQLSLDAPAPVAAWQRSGDPRGAITLRHLLHMSSGLE
HVENGDPVWDGDTVAMLFGGGAADMAGFAEAKPAAAQPGEVFNYSSATSIILSDIIADTLTPSESPDARRDAMRDFIAGR
LIEPLGMTSLTPEFDAKGTMIGGSIMHATARDYARFGEFLRNDGVVNGQRLLPESWIRFMLTPSTRDAGYGGHIWLNRRR
PPGAQPALWPDRGPNDLFAAIGHQGQYIIVSPSQRVTIVRLGVTRDDQFPALRRHLADLTAAL

Sequences:

>Translated_383_residues
MKKWLLASAALAMLGAWSLTQAAGQGSLAPAAPAPRFGLSLDDVAAADASSALPALPAALRARADALFADAEAVGQTRAL
LVLRDGEPIYERYAAGFGPDSKLISWSMAKSITAVLTGFLVADGQLSLDAPAPVAAWQRSGDPRGAITLRHLLHMSSGLE
HVENGDPVWDGDTVAMLFGGGAADMAGFAEAKPAAAQPGEVFNYSSATSIILSDIIADTLTPSESPDARRDAMRDFIAGR
LIEPLGMTSLTPEFDAKGTMIGGSIMHATARDYARFGEFLRNDGVVNGQRLLPESWIRFMLTPSTRDAGYGGHIWLNRRR
PPGAQPALWPDRGPNDLFAAIGHQGQYIIVSPSQRVTIVRLGVTRDDQFPALRRHLADLTAAL
>Mature_383_residues
MKKWLLASAALAMLGAWSLTQAAGQGSLAPAAPAPRFGLSLDDVAAADASSALPALPAALRARADALFADAEAVGQTRAL
LVLRDGEPIYERYAAGFGPDSKLISWSMAKSITAVLTGFLVADGQLSLDAPAPVAAWQRSGDPRGAITLRHLLHMSSGLE
HVENGDPVWDGDTVAMLFGGGAADMAGFAEAKPAAAQPGEVFNYSSATSIILSDIIADTLTPSESPDARRDAMRDFIAGR
LIEPLGMTSLTPEFDAKGTMIGGSIMHATARDYARFGEFLRNDGVVNGQRLLPESWIRFMLTPSTRDAGYGGHIWLNRRR
PPGAQPALWPDRGPNDLFAAIGHQGQYIIVSPSQRVTIVRLGVTRDDQFPALRRHLADLTAAL

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 40604; Mature: 40604

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLLASAALAMLGAWSLTQAAGQGSLAPAAPAPRFGLSLDDVAAADASSALPALPAAL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHH
RARADALFADAEAVGQTRALLVLRDGEPIYERYAAGFGPDSKLISWSMAKSITAVLTGFL
HHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VADGQLSLDAPAPVAAWQRSGDPRGAITLRHLLHMSSGLEHVENGDPVWDGDTVAMLFGG
HCCCCEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCEEEEEECC
GAADMAGFAEAKPAAAQPGEVFNYSSATSIILSDIIADTLTPSESPDARRDAMRDFIAGR
CHHHHCCHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LIEPLGMTSLTPEFDAKGTMIGGSIMHATARDYARFGEFLRNDGVVNGQRLLPESWIRFM
HHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHHEE
LTPSTRDAGYGGHIWLNRRRPPGAQPALWPDRGPNDLFAAIGHQGQYIIVSPSQRVTIVR
ECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCEEEEEE
LGVTRDDQFPALRRHLADLTAAL
EECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKWLLASAALAMLGAWSLTQAAGQGSLAPAAPAPRFGLSLDDVAAADASSALPALPAAL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHH
RARADALFADAEAVGQTRALLVLRDGEPIYERYAAGFGPDSKLISWSMAKSITAVLTGFL
HHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VADGQLSLDAPAPVAAWQRSGDPRGAITLRHLLHMSSGLEHVENGDPVWDGDTVAMLFGG
HCCCCEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCEEEEEECC
GAADMAGFAEAKPAAAQPGEVFNYSSATSIILSDIIADTLTPSESPDARRDAMRDFIAGR
CHHHHCCHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LIEPLGMTSLTPEFDAKGTMIGGSIMHATARDYARFGEFLRNDGVVNGQRLLPESWIRFM
HHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHHEE
LTPSTRDAGYGGHIWLNRRRPPGAQPALWPDRGPNDLFAAIGHQGQYIIVSPSQRVTIVR
ECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCEEEEEE
LGVTRDDQFPALRRHLADLTAAL
EECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]