Click here to switch to the map view.

The map label for this gene is ahpF [H]

Identifier: 103486123

GI number: 103486123

Start: 640393

End: 641955

Strand: Direct

Name: ahpF [H]

Synonym: Sala_0630

Alternate gene names: 103486123

Gene position: 640393-641955 (Clockwise)

Preceding gene: 103486122

Following gene: 103486126

Centisome position: 19.14

GC content: 66.79

Gene sequence:

>1563_bases
ATGCCGATGCTCGACCCCAACACGACCGCCCAGCTCAAAACCTATCTCGGCAACCTCCGCCACCCGATCGAGCTGGTCGC
GAGCCTCGATGCCTCGCCCAAGTCGGTCGAGATGCGCGCGCTTGTCGAAGAGGTCGCGGCGCAGTCGGACCGCATCACCG
CGCGCTTCGACGGCGACGACGTACGTCGTCCGTCCTTCGCGATTCGCGGCGATGAGGGCAAAGCCGAAGCCGTGTTCGCC
GGCCTGCCGATGGGGCATGAGTTTACGTCGCTGATCCTCGCGCTGCTGCATGTCGGCGGCCACCCGCCGAAGGAAGACGC
CGAACTGCTCGACGCGGTGCGTGCGCTGGAGGGCGATTTCACCTTCGAAACCTTCTTTTCGCTGTCGTGCCAGAATTGCC
CCGACGTCGTGCAGGCGCTCAACATCATGGCGGCGCTCAATCCGAATATCCGCCACACCGCGATCGACGGCGCGCTGTTC
CAGGACGAGGTCGAACGGCGTCAGGTGATGGCGGTGCCCGCGGTGTTCCTGAACGGCGAACCTTTCGGGCAGGGGCGCAT
GGACCTCGCGCAGATCGTCGCGAAACTCGACGCCGGTGCCGTCGCCCGCGCGGCCGGAAAGATCGCCGCGAAGGAGCCGT
TCGACGTGCTCGTCGTCGGCGGCGGACCCGGCGGCGCTGCGGCGGCGATTTATGCGGCGCGCAAGGGCATCCGCACCGGC
ATCGTCGCCGAACGTTTCGGCGGGCAGCTGCTCGACACGCTGGGGATCGACAATTTCGTCTCGGTCCAGCACACCGAAGG
CCCGAAGCTCGCCGCGCAACTCGAAGCGCATGTCAAGGATTATGACGTCGATGTGATGAACGCGCAGGAAGCCGCCGCGC
TGATCCCCGGCGGCGCGAACGGCCTGCACGAGGTGAAGCTGAAAAGCGGCGCGAGCGTCAAGGGGAAGACCGTCATCCTG
TCGACCGGCGCGCGCTGGCGCCAGATGGGCGTCCCCGGCGAGGCCGAATATCGGGGCAGGGGCGTCGCCTATTGCCCGCA
TTGCGACGGTCCGCTGTTCAAGGGCAAGCGCGTCGCGGTGATCGGCGGCGGCAATTCGGGGGTCGAGGCGGCGATCGATC
TGGCCGGGCTGGTCGCGCATGTGACCTTGATCGAGTTCGACAGCCAGCTTCGCGCTGACGCGGTGCTTCAGACGAAGCTC
GGCAGCCTGCCCAATGTCACCGTCATTACGTCGGCGCTCACCACCGAAGTGCTCGGCAATGGCGAAAAGGTCGTCGGGCT
GCGCTATCGCGACCGCGGCACGGACGAGGAGCATCTGATCGAACTCGAAGGCATTTTCGTCCAGATCGGCCTTGTCCCCA
ACACCGAATGGCTCGGCGATGCGGTGGCGCTCAGCGGTCGCGGCGAGATCGAGGTCGACGCGCGCGGCGCGACCAGCCAG
CCCGGCATTTTCGCCGCGGGTGACGTGACGACGGTGCCCTACAAGCAGATCGTCATCGCGATGGGCGAGGGGTCAAAGGC
GTCGCTCTCGGCCTTCGACCATCTGATTCGCGCGGGCGCCTGA

Upstream 100 bases:

>100_bases
GGCGGGGGCCCATCTCCGGACGGAGCAAATCGCACCGGCAGGTGATGGATCCCCGCTTTCGCGGGGATGCACAACTCCTA
TTGCCTTCAGGATTCTCCCC

Downstream 100 bases:

>100_bases
CGCCGCGCACGCGACCTACCGCCCCTCCGGCCAGACGTCCGTGTCGTGGTCGGGGTGCTGATGGTTGCTCGCCTTGATCG
CGCCGACATAGGCCGCCATC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MPMLDPNTTAQLKTYLGNLRHPIELVASLDASPKSVEMRALVEEVAAQSDRITARFDGDDVRRPSFAIRGDEGKAEAVFA
GLPMGHEFTSLILALLHVGGHPPKEDAELLDAVRALEGDFTFETFFSLSCQNCPDVVQALNIMAALNPNIRHTAIDGALF
QDEVERRQVMAVPAVFLNGEPFGQGRMDLAQIVAKLDAGAVARAAGKIAAKEPFDVLVVGGGPGGAAAAIYAARKGIRTG
IVAERFGGQLLDTLGIDNFVSVQHTEGPKLAAQLEAHVKDYDVDVMNAQEAAALIPGGANGLHEVKLKSGASVKGKTVIL
STGARWRQMGVPGEAEYRGRGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGLVAHVTLIEFDSQLRADAVLQTKL
GSLPNVTVITSALTTEVLGNGEKVVGLRYRDRGTDEEHLIELEGIFVQIGLVPNTEWLGDAVALSGRGEIEVDARGATSQ
PGIFAAGDVTTVPYKQIVIAMGEGSKASLSAFDHLIRAGA

Sequences:

>Translated_520_residues
MPMLDPNTTAQLKTYLGNLRHPIELVASLDASPKSVEMRALVEEVAAQSDRITARFDGDDVRRPSFAIRGDEGKAEAVFA
GLPMGHEFTSLILALLHVGGHPPKEDAELLDAVRALEGDFTFETFFSLSCQNCPDVVQALNIMAALNPNIRHTAIDGALF
QDEVERRQVMAVPAVFLNGEPFGQGRMDLAQIVAKLDAGAVARAAGKIAAKEPFDVLVVGGGPGGAAAAIYAARKGIRTG
IVAERFGGQLLDTLGIDNFVSVQHTEGPKLAAQLEAHVKDYDVDVMNAQEAAALIPGGANGLHEVKLKSGASVKGKTVIL
STGARWRQMGVPGEAEYRGRGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGLVAHVTLIEFDSQLRADAVLQTKL
GSLPNVTVITSALTTEVLGNGEKVVGLRYRDRGTDEEHLIELEGIFVQIGLVPNTEWLGDAVALSGRGEIEVDARGATSQ
PGIFAAGDVTTVPYKQIVIAMGEGSKASLSAFDHLIRAGA
>Mature_519_residues
PMLDPNTTAQLKTYLGNLRHPIELVASLDASPKSVEMRALVEEVAAQSDRITARFDGDDVRRPSFAIRGDEGKAEAVFAG
LPMGHEFTSLILALLHVGGHPPKEDAELLDAVRALEGDFTFETFFSLSCQNCPDVVQALNIMAALNPNIRHTAIDGALFQ
DEVERRQVMAVPAVFLNGEPFGQGRMDLAQIVAKLDAGAVARAAGKIAAKEPFDVLVVGGGPGGAAAAIYAARKGIRTGI
VAERFGGQLLDTLGIDNFVSVQHTEGPKLAAQLEAHVKDYDVDVMNAQEAAALIPGGANGLHEVKLKSGASVKGKTVILS
TGARWRQMGVPGEAEYRGRGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGLVAHVTLIEFDSQLRADAVLQTKLG
SLPNVTVITSALTTEVLGNGEKVVGLRYRDRGTDEEHLIELEGIFVQIGLVPNTEWLGDAVALSGRGEIEVDARGATSQP
GIFAAGDVTTVPYKQIVIAMGEGSKASLSAFDHLIRAGA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=518, Percent_Identity=61.1969111969112, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=319, Percent_Identity=31.3479623824451, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321898, Length=313, Percent_Identity=30.6709265175719, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320560, Length=313, Percent_Identity=30.0319488817891, Blast_Score=131, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 54729; Mature: 54598

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMLDPNTTAQLKTYLGNLRHPIELVASLDASPKSVEMRALVEEVAAQSDRITARFDGDD
CCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
VRRPSFAIRGDEGKAEAVFAGLPMGHEFTSLILALLHVGGHPPKEDAELLDAVRALEGDF
CCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
TFETFFSLSCQNCPDVVQALNIMAALNPNIRHTAIDGALFQDEVERRQVMAVPAVFLNGE
EEEHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHEEEECEEEECCC
PFGQGRMDLAQIVAKLDAGAVARAAGKIAAKEPFDVLVVGGGPGGAAAAIYAARKGIRTG
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCHHC
IVAERFGGQLLDTLGIDNFVSVQHTEGPKLAAQLEAHVKDYDVDVMNAQEAAALIPGGAN
HHHHHHCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEECCCHHHEECCCCCC
GLHEVKLKSGASVKGKTVILSTGARWRQMGVPGEAEYRGRGVAYCPHCDGPLFKGKRVAV
CCEEEEECCCCCCCCCEEEEECCCHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGLVAHVTLIEFDSQLRADAVLQTKLGSLPNVTVITSALTTEVLGN
EECCCCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCC
GEKVVGLRYRDRGTDEEHLIELEGIFVQIGLVPNTEWLGDAVALSGRGEIEVDARGATSQ
CCEEEEEEECCCCCCHHHEEEEEEEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCC
PGIFAAGDVTTVPYKQIVIAMGEGSKASLSAFDHLIRAGA
CCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PMLDPNTTAQLKTYLGNLRHPIELVASLDASPKSVEMRALVEEVAAQSDRITARFDGDD
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
VRRPSFAIRGDEGKAEAVFAGLPMGHEFTSLILALLHVGGHPPKEDAELLDAVRALEGDF
CCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
TFETFFSLSCQNCPDVVQALNIMAALNPNIRHTAIDGALFQDEVERRQVMAVPAVFLNGE
EEEHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHEEEECEEEECCC
PFGQGRMDLAQIVAKLDAGAVARAAGKIAAKEPFDVLVVGGGPGGAAAAIYAARKGIRTG
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCHHC
IVAERFGGQLLDTLGIDNFVSVQHTEGPKLAAQLEAHVKDYDVDVMNAQEAAALIPGGAN
HHHHHHCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEECCCHHHEECCCCCC
GLHEVKLKSGASVKGKTVILSTGARWRQMGVPGEAEYRGRGVAYCPHCDGPLFKGKRVAV
CCEEEEECCCCCCCCCEEEEECCCHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGLVAHVTLIEFDSQLRADAVLQTKLGSLPNVTVITSALTTEVLGN
EECCCCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCC
GEKVVGLRYRDRGTDEEHLIELEGIFVQIGLVPNTEWLGDAVALSGRGEIEVDARGATSQ
CCEEEEEEECCCCCCHHHEEEEEEEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCC
PGIFAAGDVTTVPYKQIVIAMGEGSKASLSAFDHLIRAGA
CCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]