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The map label for this gene is ispB [H]

Identifier: 103486007

GI number: 103486007

Start: 531457

End: 532470

Strand: Direct

Name: ispB [H]

Synonym: Sala_0514

Alternate gene names: 103486007

Gene position: 531457-532470 (Clockwise)

Preceding gene: 103486004

Following gene: 103486008

Centisome position: 15.89

GC content: 65.78

Gene sequence:

>1014_bases
ATGACTGCCGAAATCCACCAGCTTCGTGGGACTGTCCCGCCCTCGCTCGACCCGATGATGACGCTTGTCGCCGCCGACAT
GAACGAGGTGAATGCGGTCATCCTCGACCGGATGCAGTCCGAAGTGCCGCTGATCCCCGAACTCGCCGGCCATCTGATTG
CGGGTGGCGGCAAAAGGATGCGGCCAATGCTGACGCTCGCCTGCGGGCGTCTGCTCGACTATCCGGGGCGCCGCCATTGC
AAACTCGCCGCGGCCGTCGAGTTCATCCACACCGCGACCCTGCTCCACGACGATGTCGTCGACAAATCGGACCTGCGCCG
CGGCCGCAAGACCGCGAATGTCATCTGGGGCAACAGCGCCTCGGTGCTCGTCGGCGACTTCCTGTTCAGCCGCGCCTTCG
AGGTGATGGTCGAGGACGGCTCGCTGAAGGTGCTCAAGATATTGAGCCGCGCCTCGGCGGTGATCGCCGAGGGCGAGGTC
AACCAGCTCTCCGCGCAGCGCCGCATCGAAACGAGCGAGGACCAGTATCTCGCGATCATCAACGCCAAGACCGCCGAACT
GTTCGCCGCCGCGTGCAAAATCGCCGCGGTCGTCGCCGAACGCGACGAAGCGACCGAGGCCGCGCTCGACGCCTATGGCC
GAAATCTGGGCATCGCCTTCCAGCTCGTCGACGACGCCATCGACTATGTCTCCGACGCCGAAACAATGGGCAAAGGCGTC
GGCGACGATTTCCGCGACGGCAAGGTCACGCTGCCCGTCATCCTCGCCTATGCGCGCGGGTCGGCCGAGGAGCGCGATTT
CTGGAAGCAGGCGATCACCGGCCACAAGACGTCGGACGAGGATCTGGTCTACGCCACCAGCCTGCTGCGCAAGCATGACA
CGATCGCCGACACGCTGGCGCGCGCGCGCCATTATGGCCAGCGGGCGGTCGATGCGATCGGCGGTTTCCGCGCAAGCCAG
GCGAAGTCGGCGCTGACCGAAGCGGTCGCCTTCGCGGTCGCGCGCGCTTATTGA

Upstream 100 bases:

>100_bases
GGCTCCTGTTTCTTGCGGCCCGCTTGGCACCGGCAAGGGGGCGGTGGCAAGCCGAAAGTCGTTGCAAAATCGCAGCCGCG
CCGCCAAGGGTCGCGGCGAT

Downstream 100 bases:

>100_bases
AACGGGGATCGCGGCGACGATGGGTCCGACGGCGGAAAGCCCCGACGCCTATATCGCCGCGCTTTCGGGCTGGCAGCTCG
ACCGCTGCACGATGATGCGC

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTAEIHQLRGTVPPSLDPMMTLVAADMNEVNAVILDRMQSEVPLIPELAGHLIAGGGKRMRPMLTLACGRLLDYPGRRHC
KLAAAVEFIHTATLLHDDVVDKSDLRRGRKTANVIWGNSASVLVGDFLFSRAFEVMVEDGSLKVLKILSRASAVIAEGEV
NQLSAQRRIETSEDQYLAIINAKTAELFAAACKIAAVVAERDEATEAALDAYGRNLGIAFQLVDDAIDYVSDAETMGKGV
GDDFRDGKVTLPVILAYARGSAEERDFWKQAITGHKTSDEDLVYATSLLRKHDTIADTLARARHYGQRAVDAIGGFRASQ
AKSALTEAVAFAVARAY

Sequences:

>Translated_337_residues
MTAEIHQLRGTVPPSLDPMMTLVAADMNEVNAVILDRMQSEVPLIPELAGHLIAGGGKRMRPMLTLACGRLLDYPGRRHC
KLAAAVEFIHTATLLHDDVVDKSDLRRGRKTANVIWGNSASVLVGDFLFSRAFEVMVEDGSLKVLKILSRASAVIAEGEV
NQLSAQRRIETSEDQYLAIINAKTAELFAAACKIAAVVAERDEATEAALDAYGRNLGIAFQLVDDAIDYVSDAETMGKGV
GDDFRDGKVTLPVILAYARGSAEERDFWKQAITGHKTSDEDLVYATSLLRKHDTIADTLARARHYGQRAVDAIGGFRASQ
AKSALTEAVAFAVARAY
>Mature_336_residues
TAEIHQLRGTVPPSLDPMMTLVAADMNEVNAVILDRMQSEVPLIPELAGHLIAGGGKRMRPMLTLACGRLLDYPGRRHCK
LAAAVEFIHTATLLHDDVVDKSDLRRGRKTANVIWGNSASVLVGDFLFSRAFEVMVEDGSLKVLKILSRASAVIAEGEVN
QLSAQRRIETSEDQYLAIINAKTAELFAAACKIAAVVAERDEATEAALDAYGRNLGIAFQLVDDAIDYVSDAETMGKGVG
DDFRDGKVTLPVILAYARGSAEERDFWKQAITGHKTSDEDLVYATSLLRKHDTIADTLARARHYGQRAVDAIGGFRASQA
KSALTEAVAFAVARAY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=298, Percent_Identity=30.2013422818792, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4758430, Length=275, Percent_Identity=24, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI83700220, Length=275, Percent_Identity=24, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI169808399, Length=261, Percent_Identity=23.7547892720307, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789578, Length=258, Percent_Identity=49.2248062015504, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1786623, Length=229, Percent_Identity=33.1877729257642, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=232, Percent_Identity=35.3448275862069, Blast_Score=141, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6319475, Length=303, Percent_Identity=31.6831683168317, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=286, Percent_Identity=22.027972027972, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24651612, Length=222, Percent_Identity=34.6846846846847, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281365769, Length=238, Percent_Identity=25.6302521008403, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24660002, Length=238, Percent_Identity=25.6302521008403, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36514; Mature: 36383

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEIHQLRGTVPPSLDPMMTLVAADMNEVNAVILDRMQSEVPLIPELAGHLIAGGGKRM
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
RPMLTLACGRLLDYPGRRHCKLAAAVEFIHTATLLHDDVVDKSDLRRGRKTANVIWGNSA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCC
SVLVGDFLFSRAFEVMVEDGSLKVLKILSRASAVIAEGEVNQLSAQRRIETSEDQYLAII
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEE
NAKTAELFAAACKIAAVVAERDEATEAALDAYGRNLGIAFQLVDDAIDYVSDAETMGKGV
ECHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GDDFRDGKVTLPVILAYARGSAEERDFWKQAITGHKTSDEDLVYATSLLRKHDTIADTLA
CCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RARHYGQRAVDAIGGFRASQAKSALTEAVAFAVARAY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAEIHQLRGTVPPSLDPMMTLVAADMNEVNAVILDRMQSEVPLIPELAGHLIAGGGKRM
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
RPMLTLACGRLLDYPGRRHCKLAAAVEFIHTATLLHDDVVDKSDLRRGRKTANVIWGNSA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCC
SVLVGDFLFSRAFEVMVEDGSLKVLKILSRASAVIAEGEVNQLSAQRRIETSEDQYLAII
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEE
NAKTAELFAAACKIAAVVAERDEATEAALDAYGRNLGIAFQLVDDAIDYVSDAETMGKGV
ECHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GDDFRDGKVTLPVILAYARGSAEERDFWKQAITGHKTSDEDLVYATSLLRKHDTIADTLA
CCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RARHYGQRAVDAIGGFRASQAKSALTEAVAFAVARAY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]