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The map label for this gene is baiB [H]

Identifier: 103485717

GI number: 103485717

Start: 229680

End: 231212

Strand: Direct

Name: baiB [H]

Synonym: Sala_0221

Alternate gene names: 103485717

Gene position: 229680-231212 (Clockwise)

Preceding gene: 103485715

Following gene: 103485718

Centisome position: 6.87

GC content: 63.93

Gene sequence:

>1533_bases
ATGCACCCCTCAGTCCACGCCCGCACCAACCCTGAAAAGGCCGCGATCATCGTCGCCGAGACGGGAGAGGAAATCAGCTA
TGGCGACCTTGACGCCGCCTCGAACCGCGCCGCGCAGCTGTTCCGCGCCAACGGGCTGGGGCATGAGGATGTCGTTGCCT
TCATGCTCGACAACACGCCGCATTATTACGGCCTGACCTGGGGCGCGCAGCGCGCGGGGCTGCGCTATGTCTGCATCTCG
TCGCGGCTGACGCAGGACGAGACCGACTATATCCTGGACAATTCGGGCGCGCAGATCCTCGTCGTCTCGGCCAGTCTCGC
CGACGCCGCGCAGCGGCTGACGACCGGCATCAAGCGCTTTGTGATGGGCGGCGCCATCGCGGGTTATGAAAGCTGGGAGG
ATGCCGTCGCGGCCATGCCCGCGACGCCCGTTGCCGACGAGCGCGCGGGGGTTGACATGCTCTATTCGTCCGGGACCACC
GGGCGCCCCAAGGGTGTGCGCGTTCCGCTGCCCGAGGATCCGGCGATCGACGCGACGAACAGCCTGGTCATGCTCGCTTC
GGCAGTGTTCCAGATCAATGCGGACAGCATCTATCTCTCGCCCGCGCCGCTCTATCACGCCGCGCCGCTCCGCTGGTCGA
TGACGATCCACCGGCTCGGCGGCACCGTCGTCCTGATGAAGAAGTTCGATCCCGAAGCGGCGCTGGCGCACATCGAACGC
TATCGCGTGAACAGCAGCCAATGGGTGCCGACGCATTTCGTGCGGATGCTGAAACTGCCGGAGGAGGTGCGGAACCGCTA
CGATCTTTCGTCGCTCAAGGTCGCGATCCATGCCGCCGCGCCGTGCCCGGTGCCGGTGAAGCAGGCGATGATCGACTGGT
GGGGACCGGTATTGTATGAATATTATGCCGGATCCGAAGGCAATGGCATGACCTTCATCTCCAGCACCGACTGGCTGACG
CACAAGGGCAGCGTCGGGCGCCCGATCCTCGGCGCCGTGCATATCATGGGCGAGGACAATGAAACCGAACTCGGCGTGGG
CGAGGAAGGCACGATCTTTTTCGAGAGCGAGAATGTCTTCGAATATCATGGCGACGACGAAAAAACGGCGTCGAGCCGCA
ATTCGAAAGGCTGGTCGACGCTCGGCGATGTGGGCAGGCTCGACGAGGAGGGCTTTCTCTACCTCACCGACCGCAAGAGC
TTCATGATCATCTCGGGCGGGGTGAACATCTATCCGCAGGAAATCGAGAATCACCTTGTCACCCATCCCAAGGTCGCCGA
CGTCGCGGTGGTCGGCGGTCCGCACGAGGAGATGGGCGAGGAGGTGATCGCGGTGGTCCAGCCCGCCGACATGGCCGAGG
CGGGCGACGCGCTGCGCGACGAGCTCATCGCCTATGCGCGCGAAAAACTGTCGGGGGTGAAGATTCCGCGCCGTATCGAC
TTCCTCGAAGCCTTGCCGCGCCATGACACGGGCAAGCTCTACAAACGCCTGCTTCGCGACCGATATTGGGAGAAGGCGAA
GGCGGAGGCCTGA

Upstream 100 bases:

>100_bases
CTTCGAACCTGTCACGCCATCCAGCTTGCTTGACCAAAAGTTTCAGCTTGCTACTCATCTTCGACATAAAGAAACCAGCG
ATTCCCGGAGATGATGCCCG

Downstream 100 bases:

>100_bases
TGCAATGACCGCCGCGCGCGTCGAGTTTTTCTTCGATCTATCCTCGCCCTGGACCTGCCTCGCCTTTCACAACCTGCCTG
CCGTGCTCGAACGGACAGGC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MHPSVHARTNPEKAAIIVAETGEEISYGDLDAASNRAAQLFRANGLGHEDVVAFMLDNTPHYYGLTWGAQRAGLRYVCIS
SRLTQDETDYILDNSGAQILVVSASLADAAQRLTTGIKRFVMGGAIAGYESWEDAVAAMPATPVADERAGVDMLYSSGTT
GRPKGVRVPLPEDPAIDATNSLVMLASAVFQINADSIYLSPAPLYHAAPLRWSMTIHRLGGTVVLMKKFDPEAALAHIER
YRVNSSQWVPTHFVRMLKLPEEVRNRYDLSSLKVAIHAAAPCPVPVKQAMIDWWGPVLYEYYAGSEGNGMTFISSTDWLT
HKGSVGRPILGAVHIMGEDNETELGVGEEGTIFFESENVFEYHGDDEKTASSRNSKGWSTLGDVGRLDEEGFLYLTDRKS
FMIISGGVNIYPQEIENHLVTHPKVADVAVVGGPHEEMGEEVIAVVQPADMAEAGDALRDELIAYAREKLSGVKIPRRID
FLEALPRHDTGKLYKRLLRDRYWEKAKAEA

Sequences:

>Translated_510_residues
MHPSVHARTNPEKAAIIVAETGEEISYGDLDAASNRAAQLFRANGLGHEDVVAFMLDNTPHYYGLTWGAQRAGLRYVCIS
SRLTQDETDYILDNSGAQILVVSASLADAAQRLTTGIKRFVMGGAIAGYESWEDAVAAMPATPVADERAGVDMLYSSGTT
GRPKGVRVPLPEDPAIDATNSLVMLASAVFQINADSIYLSPAPLYHAAPLRWSMTIHRLGGTVVLMKKFDPEAALAHIER
YRVNSSQWVPTHFVRMLKLPEEVRNRYDLSSLKVAIHAAAPCPVPVKQAMIDWWGPVLYEYYAGSEGNGMTFISSTDWLT
HKGSVGRPILGAVHIMGEDNETELGVGEEGTIFFESENVFEYHGDDEKTASSRNSKGWSTLGDVGRLDEEGFLYLTDRKS
FMIISGGVNIYPQEIENHLVTHPKVADVAVVGGPHEEMGEEVIAVVQPADMAEAGDALRDELIAYAREKLSGVKIPRRID
FLEALPRHDTGKLYKRLLRDRYWEKAKAEA
>Mature_510_residues
MHPSVHARTNPEKAAIIVAETGEEISYGDLDAASNRAAQLFRANGLGHEDVVAFMLDNTPHYYGLTWGAQRAGLRYVCIS
SRLTQDETDYILDNSGAQILVVSASLADAAQRLTTGIKRFVMGGAIAGYESWEDAVAAMPATPVADERAGVDMLYSSGTT
GRPKGVRVPLPEDPAIDATNSLVMLASAVFQINADSIYLSPAPLYHAAPLRWSMTIHRLGGTVVLMKKFDPEAALAHIER
YRVNSSQWVPTHFVRMLKLPEEVRNRYDLSSLKVAIHAAAPCPVPVKQAMIDWWGPVLYEYYAGSEGNGMTFISSTDWLT
HKGSVGRPILGAVHIMGEDNETELGVGEEGTIFFESENVFEYHGDDEKTASSRNSKGWSTLGDVGRLDEEGFLYLTDRKS
FMIISGGVNIYPQEIENHLVTHPKVADVAVVGGPHEEMGEEVIAVVQPADMAEAGDALRDELIAYAREKLSGVKIPRRID
FLEALPRHDTGKLYKRLLRDRYWEKAKAEA

Specific function: Active with a large number of C-24 bile acids possessing unhindered C-24 carboxyl groups. Requires ATP and produces AMP and pyrophosphate in addition to the bile-acid CoA thioester [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=534, Percent_Identity=26.2172284644195, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI115511026, Length=526, Percent_Identity=26.0456273764259, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI187761345, Length=520, Percent_Identity=24.8076923076923, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI187761343, Length=520, Percent_Identity=24.8076923076923, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI38505220, Length=530, Percent_Identity=25.8490566037736, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI42544132, Length=525, Percent_Identity=25.3333333333333, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI157311624, Length=530, Percent_Identity=25.8490566037736, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI157311622, Length=530, Percent_Identity=25.8490566037736, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI58082049, Length=537, Percent_Identity=24.9534450651769, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI122937307, Length=516, Percent_Identity=25, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI38524616, Length=547, Percent_Identity=26.3254113345521, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI40807357, Length=566, Percent_Identity=25.0883392226148, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI227499619, Length=471, Percent_Identity=22.9299363057325, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI28416953, Length=560, Percent_Identity=22.3214285714286, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI8923896, Length=378, Percent_Identity=23.5449735449735, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI253970489, Length=378, Percent_Identity=23.5449735449735, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI62865631, Length=546, Percent_Identity=23.0769230769231, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI13325055, Length=546, Percent_Identity=23.0769230769231, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788107, Length=538, Percent_Identity=26.3940520446097, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI221142682, Length=511, Percent_Identity=26.8101761252446, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI145693145, Length=521, Percent_Identity=25.9117082533589, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1790505, Length=563, Percent_Identity=22.9129662522202, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1786810, Length=524, Percent_Identity=26.5267175572519, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786529, Length=375, Percent_Identity=25.8666666666667, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=505, Percent_Identity=27.1287128712871, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17531443, Length=532, Percent_Identity=25.7518796992481, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=503, Percent_Identity=25.2485089463221, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17559526, Length=484, Percent_Identity=27.8925619834711, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=358, Percent_Identity=27.6536312849162, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=358, Percent_Identity=27.6536312849162, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=358, Percent_Identity=27.6536312849162, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=537, Percent_Identity=24.7672253258845, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=525, Percent_Identity=23.8095238095238, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=510, Percent_Identity=24.1176470588235, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17551278, Length=565, Percent_Identity=23.716814159292, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560308, Length=335, Percent_Identity=24.4776119402985, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=362, Percent_Identity=26.7955801104972, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323182, Length=585, Percent_Identity=26.3247863247863, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355181, Length=520, Percent_Identity=27.1153846153846, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI18859661, Length=523, Percent_Identity=27.151051625239, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648255, Length=449, Percent_Identity=26.0579064587973, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648253, Length=450, Percent_Identity=26.2222222222222, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356947, Length=514, Percent_Identity=23.9299610894942, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24581924, Length=536, Percent_Identity=24.8134328358209, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653035, Length=513, Percent_Identity=21.6374269005848, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI20130357, Length=518, Percent_Identity=24.7104247104247, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI161076582, Length=523, Percent_Identity=21.2237093690249, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648260, Length=524, Percent_Identity=23.2824427480916, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356441, Length=523, Percent_Identity=23.5181644359465, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922652, Length=529, Percent_Identity=22.8733459357278, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24667955, Length=381, Percent_Identity=25.1968503937008, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24667959, Length=381, Percent_Identity=25.1968503937008, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI62472339, Length=381, Percent_Identity=25.1968503937008, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21358303, Length=476, Percent_Identity=22.4789915966387, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281365686, Length=490, Percent_Identity=23.8775510204082, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648257, Length=491, Percent_Identity=23.8289205702648, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24656500, Length=511, Percent_Identity=22.1135029354207, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355225, Length=376, Percent_Identity=24.7340425531915, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 56017; Mature: 56017

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPSVHARTNPEKAAIIVAETGEEISYGDLDAASNRAAQLFRANGLGHEDVVAFMLDNTP
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHCHHHHHHHHHCCCCHHHEEEEEECCCC
HYYGLTWGAQRAGLRYVCISSRLTQDETDYILDNSGAQILVVSASLADAAQRLTTGIKRF
CEEEEECCCCCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHH
VMGGAIAGYESWEDAVAAMPATPVADERAGVDMLYSSGTTGRPKGVRVPLPEDPAIDATN
HHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCEECCCCCCCCCCCCC
SLVMLASAVFQINADSIYLSPAPLYHAAPLRWSMTIHRLGGTVVLMKKFDPEAALAHIER
HHHHHHHHHEEECCCEEEECCCCCCCCCCCEEEEEEEECCCEEEEEEECCCHHHHHHHHH
YRVNSSQWVPTHFVRMLKLPEEVRNRYDLSSLKVAIHAAAPCPVPVKQAMIDWWGPVLYE
HCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
YYAGSEGNGMTFISSTDWLTHKGSVGRPILGAVHIMGEDNETELGVGEEGTIFFESENVF
HHCCCCCCCEEEEECCCCEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCEE
EYHGDDEKTASSRNSKGWSTLGDVGRLDEEGFLYLTDRKSFMIISGGVNIYPQEIENHLV
EECCCCHHHHCCCCCCCCCHHHHHCCCCCCCEEEEECCCEEEEEECCCEECHHHHHHHHC
THPKVADVAVVGGPHEEMGEEVIAVVQPADMAEAGDALRDELIAYAREKLSGVKIPRRID
CCCCCCEEEEECCCHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FLEALPRHDTGKLYKRLLRDRYWEKAKAEA
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHPSVHARTNPEKAAIIVAETGEEISYGDLDAASNRAAQLFRANGLGHEDVVAFMLDNTP
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHCHHHHHHHHHCCCCHHHEEEEEECCCC
HYYGLTWGAQRAGLRYVCISSRLTQDETDYILDNSGAQILVVSASLADAAQRLTTGIKRF
CEEEEECCCCCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHH
VMGGAIAGYESWEDAVAAMPATPVADERAGVDMLYSSGTTGRPKGVRVPLPEDPAIDATN
HHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCEECCCCCCCCCCCCC
SLVMLASAVFQINADSIYLSPAPLYHAAPLRWSMTIHRLGGTVVLMKKFDPEAALAHIER
HHHHHHHHHEEECCCEEEECCCCCCCCCCCEEEEEEEECCCEEEEEEECCCHHHHHHHHH
YRVNSSQWVPTHFVRMLKLPEEVRNRYDLSSLKVAIHAAAPCPVPVKQAMIDWWGPVLYE
HCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
YYAGSEGNGMTFISSTDWLTHKGSVGRPILGAVHIMGEDNETELGVGEEGTIFFESENVF
HHCCCCCCCEEEEECCCCEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCEE
EYHGDDEKTASSRNSKGWSTLGDVGRLDEEGFLYLTDRKSFMIISGGVNIYPQEIENHLV
EECCCCHHHHCCCCCCCCCHHHHHCCCCCCCEEEEECCCEEEEEECCCEECHHHHHHHHC
THPKVADVAVVGGPHEEMGEEVIAVVQPADMAEAGDALRDELIAYAREKLSGVKIPRRID
CCCCCCEEEEECCCHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FLEALPRHDTGKLYKRLLRDRYWEKAKAEA
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254270; 1551828 [H]