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The map label for this gene is tnpR [H]

Identifier: 145301505

GI number: 145301505

Start: 120259

End: 121176

Strand: Direct

Name: tnpR [H]

Synonym: ASA_P5G127

Alternate gene names: 145301505

Gene position: 120259-121176 (Clockwise)

Preceding gene: 145301500

Following gene: 145301506

Centisome position: 77.54

GC content: 58.06

Gene sequence:

>918_bases
ATGAAAGTAGGCTATGCACGGGTATCCACCCGCGACCAAAATCCAGACATGCAGGTGGACGCGCTCAATGCGGCTGGCTG
CGAGCGCATTTATCAGGATGTGGCCAGCGGAGCGAAAACAGCACGCCCGGCATTTGACGAGCTGCAGCGGCAGTTACGCG
CAGGCGACGTGTTGGTTGTCTGGAAACTTGATCGGATGGGGCGTTCGCTCAAGCACCTGGTGGAACTAGTGGGCAGCCTG
ATGGAGCGTAAAGTGGGCCTGCTCAGCCTGAATGACCCGGTCGATACCACCAACGCGCAAGGGAGACTGGTATTTAACCT
GTTCGCCTCGCTGGCCGAGTTCGAGCGTGAACTGATCCGCGAGCGCACCCTGGCAGGACTGACAGCAGTGCGGGCCCGCG
GACGAGTAGGTGGACGACCCAGAGGCTTGTCACCAGAGGCGGAGGCTACGGCCCTCGCAGCGGAGACCTTGTACCGTGAG
GGCACACTGTCGGTCGCAGCCATCGCACAAAAGCTGCATTTGTCCAAGAGTACCCTGTACAGCTACCTGCGGCATCGTGG
CGTGGAGATTGGCCCGCATAAACAATCGAGACAGCAGCTGCCAAACATGCAACACGTCGAGAGCGGCGATCACTCCAAGG
TTGCAACCATCCTGCTGACGCTGCGCATTGAGAACAACAGCAAGTTTGTGCGGGGGAAGAAGCGCACGATAGAGCAAGTT
GAGTTTTTTGAGCTTGCCCACTACGAGGCCACGCGGCGGGCGAACGGGGAATATGAGCTGAAGGTGCCCTATGACACTGA
GGAAGAGTTGGATAAAACCATGAACGATTTGCTAACCGACATAGGTTGCGTTGCGGATGATCGGCACTGCTTCTCGGAGA
GCTATGCCCGCATGGAAGGCTCAGATCGTTGTTGGTGA

Upstream 100 bases:

>100_bases
GTTTAACGGCATTGATAGGTCGATAAGTGGTTCATAAAACTGGTTTGCCACAGCAAAGTTCGATAATTTGTTGGTGCAAT
GAGTTTATTGGAATTTAACC

Downstream 100 bases:

>100_bases
AGGCTTAAGTGACTTCCCTGTTCCATTGCTCCCCGAACCCCAATTACCAAAAATCCGAACGGACACAGCAAGAAGCGGCG
CGGCGCGAAGAGGTCAACAT

Product: Tn5045 resolvase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKVGYARVSTRDQNPDMQVDALNAAGCERIYQDVASGAKTARPAFDELQRQLRAGDVLVVWKLDRMGRSLKHLVELVGSL
MERKVGLLSLNDPVDTTNAQGRLVFNLFASLAEFERELIRERTLAGLTAVRARGRVGGRPRGLSPEAEATALAAETLYRE
GTLSVAAIAQKLHLSKSTLYSYLRHRGVEIGPHKQSRQQLPNMQHVESGDHSKVATILLTLRIENNSKFVRGKKRTIEQV
EFFELAHYEATRRANGEYELKVPYDTEEELDKTMNDLLTDIGCVADDRHCFSESYARMEGSDRCW

Sequences:

>Translated_305_residues
MKVGYARVSTRDQNPDMQVDALNAAGCERIYQDVASGAKTARPAFDELQRQLRAGDVLVVWKLDRMGRSLKHLVELVGSL
MERKVGLLSLNDPVDTTNAQGRLVFNLFASLAEFERELIRERTLAGLTAVRARGRVGGRPRGLSPEAEATALAAETLYRE
GTLSVAAIAQKLHLSKSTLYSYLRHRGVEIGPHKQSRQQLPNMQHVESGDHSKVATILLTLRIENNSKFVRGKKRTIEQV
EFFELAHYEATRRANGEYELKVPYDTEEELDKTMNDLLTDIGCVADDRHCFSESYARMEGSDRCW
>Mature_305_residues
MKVGYARVSTRDQNPDMQVDALNAAGCERIYQDVASGAKTARPAFDELQRQLRAGDVLVVWKLDRMGRSLKHLVELVGSL
MERKVGLLSLNDPVDTTNAQGRLVFNLFASLAEFERELIRERTLAGLTAVRARGRVGGRPRGLSPEAEATALAAETLYRE
GTLSVAAIAQKLHLSKSTLYSYLRHRGVEIGPHKQSRQQLPNMQHVESGDHSKVATILLTLRIENNSKFVRGKKRTIEQV
EFFELAHYEATRRANGEYELKVPYDTEEELDKTMNDLLTDIGCVADDRHCFSESYARMEGSDRCW

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=151, Percent_Identity=56.2913907284768, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787638, Length=184, Percent_Identity=32.0652173913043, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787827, Length=184, Percent_Identity=32.0652173913043, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 34306; Mature: 34306

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGYARVSTRDQNPDMQVDALNAAGCERIYQDVASGAKTARPAFDELQRQLRAGDVLVV
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEE
WKLDRMGRSLKHLVELVGSLMERKVGLLSLNDPVDTTNAQGRLVFNLFASLAEFERELIR
EEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ERTLAGLTAVRARGRVGGRPRGLSPEAEATALAAETLYREGTLSVAAIAQKLHLSKSTLY
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SYLRHRGVEIGPHKQSRQQLPNMQHVESGDHSKVATILLTLRIENNSKFVRGKKRTIEQV
HHHHHCCCCCCCCHHHHHHCCCCHHHCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHH
EFFELAHYEATRRANGEYELKVPYDTEEELDKTMNDLLTDIGCVADDRHCFSESYARMEG
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
SDRCW
CCCCC
>Mature Secondary Structure
MKVGYARVSTRDQNPDMQVDALNAAGCERIYQDVASGAKTARPAFDELQRQLRAGDVLVV
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEE
WKLDRMGRSLKHLVELVGSLMERKVGLLSLNDPVDTTNAQGRLVFNLFASLAEFERELIR
EEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ERTLAGLTAVRARGRVGGRPRGLSPEAEATALAAETLYREGTLSVAAIAQKLHLSKSTLY
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SYLRHRGVEIGPHKQSRQQLPNMQHVESGDHSKVATILLTLRIENNSKFVRGKKRTIEQV
HHHHHCCCCCCCCHHHHHHCCCCHHHCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHH
EFFELAHYEATRRANGEYELKVPYDTEEELDKTMNDLLTDIGCVADDRHCFSESYARMEG
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
SDRCW
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]