Click here to switch to the map view.

The map label for this gene is ascF [H]

Identifier: 145301437

GI number: 145301437

Start: 57000

End: 57257

Strand: Reverse

Name: ascF [H]

Synonym: ASA_P5G054

Alternate gene names: 145301437

Gene position: 57257-57000 (Counterclockwise)

Preceding gene: 145301438

Following gene: 145301436

Centisome position: 36.92

GC content: 49.61

Gene sequence:

>258_bases
ATGGCTGATTACTTTACTTATACGGACAAGAACAGTAACACCCTCGATCAAGTGGCATCAAACCTCTCCGGGCAGGCCAA
TGACGCCAACACCGAGGTCAACAAGGCCATTGAGGCGATGAAAACCAACCCAGATAACCCGGCGTTGTTGGCCGAACTGC
AGCACAAGATCAACAAATGGTCGGTGATCTACAACATCAACTCCACCGTGACTCGGGCCATGAAAGACCTGATGCAGGGT
ATTTTGCAGAAGATTTGA

Upstream 100 bases:

>100_bases
CTGAGCGGGGGAACCCCGCAACAGTATCGGGAGTGGAAAGAACAGGCCGACGCCATCGAGGCGGGGCTCACGATCATTGG
CAATTTAAAGGAGCACAACC

Downstream 100 bases:

>100_bases
TGATGAACCCTCAACTGAAGCGACAGCTGGCCGAGCTTGCGCTGGCTGGTACCGGTCACCATTGCCATCAGGAGGCTGCC
ACCATCGCCGACTGGCTGGC

Product: AscF protein

Products: NA

Alternate protein names: Pseudomonas secretion protein F [H]

Number of amino acids: Translated: 85; Mature: 84

Protein sequence:

>85_residues
MADYFTYTDKNSNTLDQVASNLSGQANDANTEVNKAIEAMKTNPDNPALLAELQHKINKWSVIYNINSTVTRAMKDLMQG
ILQKI

Sequences:

>Translated_85_residues
MADYFTYTDKNSNTLDQVASNLSGQANDANTEVNKAIEAMKTNPDNPALLAELQHKINKWSVIYNINSTVTRAMKDLMQG
ILQKI
>Mature_84_residues
ADYFTYTDKNSNTLDQVASNLSGQANDANTEVNKAIEAMKTNPDNPALLAELQHKINKWSVIYNINSTVTRAMKDLMQGI
LQKI

Specific function: Major component of the type III secretion needle structure. Required for cytotoxicity on macrophages [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via type III secretion system (TTSS) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mxiH/prgI/yscF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021123
- InterPro:   IPR011841 [H]

Pfam domain/function: PF09392 MxiH [H]

EC number: NA

Molecular weight: Translated: 9489; Mature: 9358

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYFTYTDKNSNTLDQVASNLSGQANDANTEVNKAIEAMKTNPDNPALLAELQHKINKW
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHE
SVIYNINSTVTRAMKDLMQGILQKI
EEEEECCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ADYFTYTDKNSNTLDQVASNLSGQANDANTEVNKAIEAMKTNPDNPALLAELQHKINKW
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHE
SVIYNINSTVTRAMKDLMQGILQKI
EEEEECCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8971719; 10984043 [H]