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The map label for this gene is radA [H]

Identifier: 49183116

GI number: 49183116

Start: 92223

End: 93599

Strand: Direct

Name: radA [H]

Synonym: BAS0082

Alternate gene names: 49183116

Gene position: 92223-93599 (Clockwise)

Preceding gene: 49183115

Following gene: 49183117

Centisome position: 1.76

GC content: 39.51

Gene sequence:

>1377_bases
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAA
CAGAAGTAACAAAACCAAGGCGCCTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTCCAAGAATTT
AACCGTGTACTTGGTGGTGGGATTGTAGATGGATCCTTAGTACTTATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCGGATTCTTCATATGATGTACTATATATATCGGGTGAGGAATCAGCAAAACAGA
TTAAACTGCGTGCCGATCGTCTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGATTTACAGCGAATTGCA
ACACACATTGAAGAGATGAACCCAGCCTTTGTTGTTATTGACTCTATTCAAACAATACATTTACCTGAGGTAACATCAGC
GCCGGGGAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTATTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCAGTAAAGAATCGCTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGTCTTGCAGAAGTATTAAACCCTTCTGAGATTTTCCTTGAGGAAAGACCAGTGGGGGTTGCGG
GATCAACAGTGGTTGCTTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCTCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCGGTGTTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGACGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCTATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTCAGAGATAAATCTACAGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCGGATGGGATTGAGGTAGTCGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Upstream 100 bases:

>100_bases
ATAAGTATAGACAAACTAAGAGGGCTACGAGATAGCCCTCTTTCTTGTACGAGAAGGATAAACTGTCTATAGATGAAAGT
GAAGTGAAATATAAAACGAT

Downstream 100 bases:

>100_bases
GCTATGGAAGAAAACAAGCAACGTGTCAAAAGTATGATTAATATTTTACAACTTGTGGCCCCGGGAACACCACTGCGCGA
AGGGATAGATAATGTACTTC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Sequences:

>Translated_458_residues
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG
>Mature_457_residues
AKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEFN
RVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIAT
HIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYFE
GDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSFG
NPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLTG
EIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=456, Percent_Identity=46.2719298245614, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49891; Mature: 49760

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPR
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
RLTEVETKSEARIETKFQEFNRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSY
HHHHCCCCCHHHHHHHHHHHHHHHCCCEECCEEEEEECCCCCCCCEEEEEEHHHHCCCCC
DVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIATHIEEMNPAFVVIDSIQTIH
CEEEEECCCCCHHEEEEEEEEEECCCEEEEEECHHHHHHHHHHHHCCCEEEEEECCEEEE
LPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHEEE
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVAS
ECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHCCCHHHHEECCCCCCCCCEEEEE
MEGTRPVLVEIQALISPTSFGNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVA
CCCCCCEEEEHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCEEEEE
GGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLTGEIRRVSRIEQRVQEAAKLG
CCCEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
FQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG
HHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECC
>Mature Secondary Structure 
AKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPR
CCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
RLTEVETKSEARIETKFQEFNRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSY
HHHHCCCCCHHHHHHHHHHHHHHHCCCEECCEEEEEECCCCCCCCEEEEEEHHHHCCCCC
DVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIATHIEEMNPAFVVIDSIQTIH
CEEEEECCCCCHHEEEEEEEEEECCCEEEEEECHHHHHHHHHHHHCCCEEEEEECCEEEE
LPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHEEE
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVAS
ECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHCCCHHHHEECCCCCCCCCEEEEE
MEGTRPVLVEIQALISPTSFGNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVA
CCCCCCEEEEHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCEEEEE
GGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLTGEIRRVSRIEQRVQEAAKLG
CCCEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
FQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG
HHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]