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The map label for this gene is rsmE [H]

Identifier: 229007176

GI number: 229007176

Start: 651334

End: 652071

Strand: Reverse

Name: rsmE [H]

Synonym: Atu0657

Alternate gene names: 229007176

Gene position: 652071-651334 (Counterclockwise)

Preceding gene: 159184409

Following gene: 15887998

Centisome position: 22.95

GC content: 61.79

Gene sequence:

>738_bases
ATGCGCGCAAATTTCCGGATGCAACGCCTGTTCATCGAAGCCTCCCTTTCCGAGGGTGTGGCGCAGGAGGCGACGGGCGA
ACAGTTCAACTATCTCGCCAATGTGCTGCGCATGACCGACGGCGCGGAAATCCTGCTGTTCAACGGCCGCGACGGCGAAT
GGAAAGCGAAGCTCTCCTTTCCGAGCCGCAAGAAGATTAGCCTCGTTCCGCTCGAACAGACGCGGCCGCAACCGGATGCC
GCGGAGCTGCATTATCTCTTCGCCCCGCTGAAAGTCGGGCGCATGGATTATCTGGTGCAGAAGGCGGTGGAGATGGGTGC
TGGCCTGTTGCAGCCGGTGATGACGCAGCATGTTCAGGGCAAGATCCACAATAGCGAAAAACTGCGCGCCAATGCCATCG
AGGCCGCCGAACAATGCGGCATTCTGTCCCTGCCCGAAGTGGCAGAGCCGGTGAAGCTGAAAGAGATGCTGGAGACCTGG
CCGGAAGACCGTCGCATCATCTATTGCGACGAAGGCGATGCGGGCCAAAATCCGCTGCCGATCCTGCAGGCCGTCAAGGA
ACGCAAAATCGCGCTGCTGGTCGGCCCGGAAGGCGGTTTTTCCGAGGAGGAACGGGCGTTGCTGCGCAGCCTTGCCTTCG
TCACCCCGATCCCGCTCGGGCCGCGCATATTGCGGGCCGACACGGCCGCCGTCGCCGCACTCGCCGTCATTCAGGCTGTT
ATCGGCGACTGGAACTGA

Upstream 100 bases:

>100_bases
CGCCGCCTGGGTGGTCACCGTTCCCGCAAGCGCAACACTTGCGGCGCTGCTCTACTGGCTCCTGTCCTTCCTTTTCCTCT
GACGCTGAAACCGAAATCCC

Downstream 100 bases:

>100_bases
GGGGCACCATTGCGCTTCATGCATCTTTAAAAGAAATTCATTTGCCACCGGATACGATCCGGCCCTATCTGCCAATCATA
TGCCTCGCGGCAACATTGAC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRANFRMQRLFIEASLSEGVAQEATGEQFNYLANVLRMTDGAEILLFNGRDGEWKAKLSFPSRKKISLVPLEQTRPQPDA
AELHYLFAPLKVGRMDYLVQKAVEMGAGLLQPVMTQHVQGKIHNSEKLRANAIEAAEQCGILSLPEVAEPVKLKEMLETW
PEDRRIIYCDEGDAGQNPLPILQAVKERKIALLVGPEGGFSEEERALLRSLAFVTPIPLGPRILRADTAAVAALAVIQAV
IGDWN

Sequences:

>Translated_245_residues
MRANFRMQRLFIEASLSEGVAQEATGEQFNYLANVLRMTDGAEILLFNGRDGEWKAKLSFPSRKKISLVPLEQTRPQPDA
AELHYLFAPLKVGRMDYLVQKAVEMGAGLLQPVMTQHVQGKIHNSEKLRANAIEAAEQCGILSLPEVAEPVKLKEMLETW
PEDRRIIYCDEGDAGQNPLPILQAVKERKIALLVGPEGGFSEEERALLRSLAFVTPIPLGPRILRADTAAVAALAVIQAV
IGDWN
>Mature_245_residues
MRANFRMQRLFIEASLSEGVAQEATGEQFNYLANVLRMTDGAEILLFNGRDGEWKAKLSFPSRKKISLVPLEQTRPQPDA
AELHYLFAPLKVGRMDYLVQKAVEMGAGLLQPVMTQHVQGKIHNSEKLRANAIEAAEQCGILSLPEVAEPVKLKEMLETW
PEDRRIIYCDEGDAGQNPLPILQAVKERKIALLVGPEGGFSEEERALLRSLAFVTPIPLGPRILRADTAAVAALAVIQAV
IGDWN

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=236, Percent_Identity=30.0847457627119, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27109; Mature: 27109

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRANFRMQRLFIEASLSEGVAQEATGEQFNYLANVLRMTDGAEILLFNGRDGEWKAKLSF
CCCCHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECC
PSRKKISLVPLEQTRPQPDAAELHYLFAPLKVGRMDYLVQKAVEMGAGLLQPVMTQHVQG
CCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
KIHNSEKLRANAIEAAEQCGILSLPEVAEPVKLKEMLETWPEDRRIIYCDEGDAGQNPLP
CCCCCHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCHH
ILQAVKERKIALLVGPEGGFSEEERALLRSLAFVTPIPLGPRILRADTAAVAALAVIQAV
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IGDWN
HCCCC
>Mature Secondary Structure
MRANFRMQRLFIEASLSEGVAQEATGEQFNYLANVLRMTDGAEILLFNGRDGEWKAKLSF
CCCCHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECC
PSRKKISLVPLEQTRPQPDAAELHYLFAPLKVGRMDYLVQKAVEMGAGLLQPVMTQHVQG
CCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
KIHNSEKLRANAIEAAEQCGILSLPEVAEPVKLKEMLETWPEDRRIIYCDEGDAGQNPLP
CCCCCHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCHH
ILQAVKERKIALLVGPEGGFSEEERALLRSLAFVTPIPLGPRILRADTAAVAALAVIQAV
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IGDWN
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]