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The map label for this gene is pit [C]

Identifier: 159184409

GI number: 159184409

Start: 652090

End: 653595

Strand: Reverse

Name: pit [C]

Synonym: Atu0658

Alternate gene names: 159184409

Gene position: 653595-652090 (Counterclockwise)

Preceding gene: 15888001

Following gene: 229007176

Centisome position: 23.0

GC content: 62.15

Gene sequence:

>1506_bases
ATGGCAACGAAGCCGCCATCGAACATGAAGCCGACGCTCGACAAGGATCTCGACAAGATTACCCGTGTCGAAGAAGCGAC
GCTGCATGTCACGCAGCAGCTTGCCGGCCTTGGCGCGGGGTTCATGTTCGTTGTGCTCGCGGCCGTCTTCGCCGGTGCTT
TCGTCACCGGCACCGCGGGCTCGGTAATCATCATCGCGGCCGTTGCCCTTGGCGCCTATATGGCCATCAACATCGGCGCA
AATGACGTGACGAACAATGTCGGCCCTGCGGTCGGCGCCAAGGCGATGCCGCTAGCGGTCGCGCTCATCGTCGCCGCCAT
CTGCGAGATCGCCGGCGCGCTGATCACCGGCGGCAATGTCGTCGAGACGATTTCGAGCGGCATCATCAACGTTGAGACTA
TTCCCGACAGGACTGCATTTTCCTGGGCGATGCTTTCGGCGCTTCTTGCTGCCGGCCTTCTCGTCAACATATCGACCTGG
ACCCGGGCGCCGATTTCCACCACGCACACGGTGGTCGGCGGGGTTGCCGGGGCGGGCATGGCCGCCTATGGGGCAAAAGC
GGTCGAATGGCTGTCGCTGGGCAGTATCGCCTTTGCCTGGGTGCTCGCGCCCTTCATCAGTGCCGCCATCGCCATCGCCA
TCCTTACTTTCATCAAGGAATTCATCGTTTACCGCGACGACAAGATCGACGCCGCGCGACGGTGGGTTCCGGTTCTCATT
GGCCTGATGGCTGGCGTTTTCATGGCCTATCTCATCTGGGTCGGATTGCGCCAGCTGATGCATGTCTCGCTTGGTCATGC
CAGTATCATAGGGCTGGTGACCGGCCTTCTCGCCTGGCGGCTCTGCGTGCCGATCATCCGCAAACAGTCGGAAGGACTGG
AAAACCGCAACCAGTCGCTTCGGATACTGTTCCAGTGGCCGCTCGTTCTCGCCGCCGGCCTGATGTCCTTTGCCCATGGG
GCAAATGACGTGTCGAACGCCATCGGTCCTGTCGTCGCCATCGTGCGGGCCCTGCACGATGAAGCGGTGCAGACATCCGC
GCGCGCGCCGCTCTGGGTGATGCTTGTCGGTGCCTTCGGGCTGTCCTGCGGCATTCTTCTCTATGGCCCGCGCCTCATCC
GCCTTGTCGGTGAACAGATCACCAAGCTCAATCCGATGCGCGCCTTTTGCGTTTCCGTGGCAACGGCGCTGACCGTTCTC
GTCGCCTCGCGTTTTGGTCTGCCTGTTAGCACCACCCATACGGCAATCGGCGCGGTGTTCGGCGTCGGCTTCTTCCGCGA
ATGGTACACACAGGCGTCAAGGCGGCGGCAGGAACTCGTCCAGTCAGGCACGGGCGAACCCCGCCGCACCCCTAGATATC
TGGACAATCCCTCCGAGGTGCGCCGCCGTTATCTTGTCCGGCGCTCGCATTTCATGACGATCATCGCCGCCTGGGTGGTC
ACCGTTCCCGCAAGCGCAACACTTGCGGCGCTGCTCTACTGGCTCCTGTCCTTCCTTTTCCTCTGA

Upstream 100 bases:

>100_bases
TTCTTTTGGAAAGATGTGCGCGATGTAACAGTCTTTCTGTAAATTGCCTTCAACCTGCGACGCTGCCGGACGAAAGGGAA
TGCTGGCAAACGAGGTGTGA

Downstream 100 bases:

>100_bases
CGCTGAAACCGAAATCCCATGCGCGCAAATTTCCGGATGCAACGCCTGTTCATCGAAGCCTCCCTTTCCGAGGGTGTGGC
GCAGGAGGCGACGGGCGAAC

Product: phosphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MATKPPSNMKPTLDKDLDKITRVEEATLHVTQQLAGLGAGFMFVVLAAVFAGAFVTGTAGSVIIIAAVALGAYMAINIGA
NDVTNNVGPAVGAKAMPLAVALIVAAICEIAGALITGGNVVETISSGIINVETIPDRTAFSWAMLSALLAAGLLVNISTW
TRAPISTTHTVVGGVAGAGMAAYGAKAVEWLSLGSIAFAWVLAPFISAAIAIAILTFIKEFIVYRDDKIDAARRWVPVLI
GLMAGVFMAYLIWVGLRQLMHVSLGHASIIGLVTGLLAWRLCVPIIRKQSEGLENRNQSLRILFQWPLVLAAGLMSFAHG
ANDVSNAIGPVVAIVRALHDEAVQTSARAPLWVMLVGAFGLSCGILLYGPRLIRLVGEQITKLNPMRAFCVSVATALTVL
VASRFGLPVSTTHTAIGAVFGVGFFREWYTQASRRRQELVQSGTGEPRRTPRYLDNPSEVRRRYLVRRSHFMTIIAAWVV
TVPASATLAALLYWLLSFLFL

Sequences:

>Translated_501_residues
MATKPPSNMKPTLDKDLDKITRVEEATLHVTQQLAGLGAGFMFVVLAAVFAGAFVTGTAGSVIIIAAVALGAYMAINIGA
NDVTNNVGPAVGAKAMPLAVALIVAAICEIAGALITGGNVVETISSGIINVETIPDRTAFSWAMLSALLAAGLLVNISTW
TRAPISTTHTVVGGVAGAGMAAYGAKAVEWLSLGSIAFAWVLAPFISAAIAIAILTFIKEFIVYRDDKIDAARRWVPVLI
GLMAGVFMAYLIWVGLRQLMHVSLGHASIIGLVTGLLAWRLCVPIIRKQSEGLENRNQSLRILFQWPLVLAAGLMSFAHG
ANDVSNAIGPVVAIVRALHDEAVQTSARAPLWVMLVGAFGLSCGILLYGPRLIRLVGEQITKLNPMRAFCVSVATALTVL
VASRFGLPVSTTHTAIGAVFGVGFFREWYTQASRRRQELVQSGTGEPRRTPRYLDNPSEVRRRYLVRRSHFMTIIAAWVV
TVPASATLAALLYWLLSFLFL
>Mature_500_residues
ATKPPSNMKPTLDKDLDKITRVEEATLHVTQQLAGLGAGFMFVVLAAVFAGAFVTGTAGSVIIIAAVALGAYMAINIGAN
DVTNNVGPAVGAKAMPLAVALIVAAICEIAGALITGGNVVETISSGIINVETIPDRTAFSWAMLSALLAAGLLVNISTWT
RAPISTTHTVVGGVAGAGMAAYGAKAVEWLSLGSIAFAWVLAPFISAAIAIAILTFIKEFIVYRDDKIDAARRWVPVLIG
LMAGVFMAYLIWVGLRQLMHVSLGHASIIGLVTGLLAWRLCVPIIRKQSEGLENRNQSLRILFQWPLVLAAGLMSFAHGA
NDVSNAIGPVVAIVRALHDEAVQTSARAPLWVMLVGAFGLSCGILLYGPRLIRLVGEQITKLNPMRAFCVSVATALTVLV
ASRFGLPVSTTHTAIGAVFGVGFFREWYTQASRRRQELVQSGTGEPRRTPRYLDNPSEVRRRYLVRRSHFMTIIAAWVVT
VPASATLAALLYWLLSFLFL

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=220, Percent_Identity=31.8181818181818, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI5803173, Length=205, Percent_Identity=33.6585365853659, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI32566716, Length=508, Percent_Identity=25.9842519685039, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI25146401, Length=490, Percent_Identity=25.7142857142857, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71981576, Length=473, Percent_Identity=27.061310782241, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17557328, Length=462, Percent_Identity=23.1601731601732, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17539280, Length=199, Percent_Identity=33.1658291457286, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536725, Length=208, Percent_Identity=28.8461538461538, Blast_Score=89, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6319773, Length=142, Percent_Identity=32.3943661971831, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356511, Length=210, Percent_Identity=34.7619047619048, Blast_Score=125, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 53399; Mature: 53268

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKPPSNMKPTLDKDLDKITRVEEATLHVTQQLAGLGAGFMFVVLAAVFAGAFVTGTAG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
SVIIIAAVALGAYMAINIGANDVTNNVGPAVGAKAMPLAVALIVAAICEIAGALITGGNV
HHHHHHHHHHHHHHEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
VETISSGIINVETIPDRTAFSWAMLSALLAAGLLVNISTWTRAPISTTHTVVGGVAGAGM
HHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEEHHHCCCCCCHHHHHHHHHHHCCH
AAYGAKAVEWLSLGSIAFAWVLAPFISAAIAIAILTFIKEFIVYRDDKIDAARRWVPVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GLMAGVFMAYLIWVGLRQLMHVSLGHASIIGLVTGLLAWRLCVPIIRKQSEGLENRNQSL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
RILFQWPLVLAAGLMSFAHGANDVSNAIGPVVAIVRALHDEAVQTSARAPLWVMLVGAFG
EEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LSCGILLYGPRLIRLVGEQITKLNPMRAFCVSVATALTVLVASRFGLPVSTTHTAIGAVF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GVGFFREWYTQASRRRQELVQSGTGEPRRTPRYLDNPSEVRRRYLVRRSHFMTIIAAWVV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TVPASATLAALLYWLLSFLFL
HCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ATKPPSNMKPTLDKDLDKITRVEEATLHVTQQLAGLGAGFMFVVLAAVFAGAFVTGTAG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
SVIIIAAVALGAYMAINIGANDVTNNVGPAVGAKAMPLAVALIVAAICEIAGALITGGNV
HHHHHHHHHHHHHHEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
VETISSGIINVETIPDRTAFSWAMLSALLAAGLLVNISTWTRAPISTTHTVVGGVAGAGM
HHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEEHHHCCCCCCHHHHHHHHHHHCCH
AAYGAKAVEWLSLGSIAFAWVLAPFISAAIAIAILTFIKEFIVYRDDKIDAARRWVPVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GLMAGVFMAYLIWVGLRQLMHVSLGHASIIGLVTGLLAWRLCVPIIRKQSEGLENRNQSL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
RILFQWPLVLAAGLMSFAHGANDVSNAIGPVVAIVRALHDEAVQTSARAPLWVMLVGAFG
EEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LSCGILLYGPRLIRLVGEQITKLNPMRAFCVSVATALTVLVASRFGLPVSTTHTAIGAVF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GVGFFREWYTQASRRRQELVQSGTGEPRRTPRYLDNPSEVRRRYLVRRSHFMTIIAAWVV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TVPASATLAALLYWLLSFLFL
HCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]