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The map label for this gene is priA [H]

Identifier: 159185300

GI number: 159185300

Start: 2609929

End: 2612145

Strand: Reverse

Name: priA [H]

Synonym: Atu2627

Alternate gene names: 159185300

Gene position: 2612145-2609929 (Counterclockwise)

Preceding gene: 15889885

Following gene: 159185299

Centisome position: 91.93

GC content: 63.55

Gene sequence:

>2217_bases
ATGGCAAAAGATTCGTCCGATCTGTTTGGGGCTCTATTTGACCCGCCGTCGCCCACGCGCACGGTGCCCGTTCTTGTGCC
CCTGCCGGCTCCGGGGCCTTATAGCTACGCCGTGCCCGAGGATATGGTGGTCGAGACCGGTTCCGTCGTGCAGGTGCCGC
TCGGTCCGCGGCAGGTTTTCGGCGTGGTGTGGGACGATGCCGGCGAGAAGGGCGTCGATCCGAAGAAGCTGCGGCCCATC
ACCAAGAGTTTCGAGTGCCCACCGCTGAAGGTGGAGATGCGCCGCTTCGTGGACTGGGTCGCTGCCTATACGCTGTCTCC
GCCCGGCCTCGTGGCGCGCATGGCGCTGCGTGCGCCTGCCGCCTTCGATCCCGAACCGATGATCGAGGGGCTGCGGCTGA
CCGAAGGCAGGCCGGAGCGCCTGACTCCCGCCCGCGAGCGGGTAATGGAGCTGGCGGCCGAAGGCCAAGGCTGGACGAAG
AGCGGACTTGCCCATGCGGCCGGCGTCTCGACCAGCGTCATCGACGGTCTCGTCAAGCAGGGCGTGTTCGAGACGGTCTT
TCTGCCCGCGCCACCTGTCGTGGCCGAGCCCGACCCTGATTATGTCGAGCCACGTCTGGAAGGCCCGCAGAAGCAGGCTG
CTTCGGAGATAATTGAAGATGTGCGCAAGGGTGGCTTTCATGTCTCGCTGATCGACGGCGTGACCGGGTCCGGCAAGACC
GAGGTTTATTTCGAGGCGGTGGCCGAAACGCTGCGACAAGGCAAGCAGGTCCTTATCCTGCTGCCGGAAATCGCCCTGAC
GGCGGCGTTTCTTGAGCGTTTTCAGGATCGCTTCGGTGCGAAACCGGCGGAATGGCATTCGGATCTGTCGCCGCGCATGC
GCGAAAAGGTCTGGCGGCAGGCGGTGACGGGTGAGATAAAGGTGGTGGCCGGCGCGCGCTCGGCGCTTTTCCTGCCCTTC
GACAATCTAGGCCTCATCATTGTCGATGAAGAGCACGACCCGGCCTACAAGCAGGAAGACCGCGTCTTTTATAATGCCCG
TGACATGGCTGTCGTGAGGGCGCGGATCGCCGAATTCCCAGTCGTGCTGGTTTCGGCCACGCCGTCGGTGGAAAGCCAGG
TCAATGGCAGTTCCGGGCGTTACAACACCATTCATCTGCATACCCGCTTCGGCGATGCGGCGATGCCGGACCTGCATCTC
GTCGATATGCGCCGCCATCCGCCGGAAAGGGGAGGGTTTCTCTCGCCCGTCCTGCTGCGCGGCATCGGCAAGACCATCGA
AAAGGGCGAGCAGTCCCTGCTCTTTCTCAATCGCCGCGGTTATGCGCCGCTGACGCTCTGCCGGGTTTGCGGTCACCGTT
TCCAATGCCCACAATGTTCCAGCTGGCTGGTGGAACACCGTTTCCGCAATCAATTGCAGTGCCACCAATGTGGCCACAAC
GAGCCGACGCCCGACCATTGCCCGGAATGCGGAACCTTCGATCATCTCGTGGCCTGTGGGCCGGGGGTGGAGCGGATCGC
CGAGGAGGTTGAGAAACATTTCCCCGAGGCCCGCACCATCGTGCTGTCTTCCGATCTCATGGGCGTCAAGCGGCTGCGTC
TGGAGCTTGAGGCGATCGTCAAGGGCGAGGCGGATATCGTCATCGGCACGCAGCTCGTCGCCAAGGGACATAACTTCCCG
CTGATGACGCTCGTCGGCATCGTCGATGCCGATCTCGGCCTTGCCAATGGCGATCCGCGCGCGGCGGAGCGGACGTTCCA
GTTGCTTTCGCAGGTGACCGGCCGTGCCGGACGAACGGGATTGAAGAGTCACGGCCTGCTGCAGACCTACCAGCCGCAGC
ACCCGGTGATGCAAGCGATCGTCTCCGGTGATGCCTCGGCCTTTTATGAAAGGGAGATCGTCGAGCGGGAAAAGGCCGTT
CTGCCGCCCTTCGGCCGCCTCGCTTCGATCATCGTCTCCGCCGATACGCGCGCGGAGGCGGAAACCCATGCGCGTGGATT
GCGAGCCGCGGCTCCGCAGGTCACCGGCATCATGCTTCTCGGCCCGGCGGAAGCGCCGCTGGCACTGATCCGTGGCCGTC
ACCGTTTTCGTCTGCTGGTGCACGGGCGGCGCAATTCCGACATGCAGTCTTTTCTTCGCACGCTTCTTGCGAGTGGCCCG
AAGGAGCGTGGCAGCGTGCATGTGCAGCTCGATATCGATCCGCAAAGTTTCCTTTGA

Upstream 100 bases:

>100_bases
CTTATGGTCAGTATTTGGTTAAGAGGTGGACTTTGCCGGGCGCGCCCACTACACCCGGGCGGTTTCCTTTTTCCTGTCGC
CGGGGCATGGTCGCCACATC

Downstream 100 bases:

>100_bases
ACTTTGCCAGAGAGGGCGTTTTCCCGCTGTCGTGACCGTGTCATAACGCGAGCGAACAACATAATGATTTGGGGAACGAG
ATGGAGTTTTATTTTCCGGC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MAKDSSDLFGALFDPPSPTRTVPVLVPLPAPGPYSYAVPEDMVVETGSVVQVPLGPRQVFGVVWDDAGEKGVDPKKLRPI
TKSFECPPLKVEMRRFVDWVAAYTLSPPGLVARMALRAPAAFDPEPMIEGLRLTEGRPERLTPARERVMELAAEGQGWTK
SGLAHAAGVSTSVIDGLVKQGVFETVFLPAPPVVAEPDPDYVEPRLEGPQKQAASEIIEDVRKGGFHVSLIDGVTGSGKT
EVYFEAVAETLRQGKQVLILLPEIALTAAFLERFQDRFGAKPAEWHSDLSPRMREKVWRQAVTGEIKVVAGARSALFLPF
DNLGLIIVDEEHDPAYKQEDRVFYNARDMAVVRARIAEFPVVLVSATPSVESQVNGSSGRYNTIHLHTRFGDAAMPDLHL
VDMRRHPPERGGFLSPVLLRGIGKTIEKGEQSLLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRNQLQCHQCGHN
EPTPDHCPECGTFDHLVACGPGVERIAEEVEKHFPEARTIVLSSDLMGVKRLRLELEAIVKGEADIVIGTQLVAKGHNFP
LMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSHGLLQTYQPQHPVMQAIVSGDASAFYEREIVEREKAV
LPPFGRLASIIVSADTRAEAETHARGLRAAAPQVTGIMLLGPAEAPLALIRGRHRFRLLVHGRRNSDMQSFLRTLLASGP
KERGSVHVQLDIDPQSFL

Sequences:

>Translated_738_residues
MAKDSSDLFGALFDPPSPTRTVPVLVPLPAPGPYSYAVPEDMVVETGSVVQVPLGPRQVFGVVWDDAGEKGVDPKKLRPI
TKSFECPPLKVEMRRFVDWVAAYTLSPPGLVARMALRAPAAFDPEPMIEGLRLTEGRPERLTPARERVMELAAEGQGWTK
SGLAHAAGVSTSVIDGLVKQGVFETVFLPAPPVVAEPDPDYVEPRLEGPQKQAASEIIEDVRKGGFHVSLIDGVTGSGKT
EVYFEAVAETLRQGKQVLILLPEIALTAAFLERFQDRFGAKPAEWHSDLSPRMREKVWRQAVTGEIKVVAGARSALFLPF
DNLGLIIVDEEHDPAYKQEDRVFYNARDMAVVRARIAEFPVVLVSATPSVESQVNGSSGRYNTIHLHTRFGDAAMPDLHL
VDMRRHPPERGGFLSPVLLRGIGKTIEKGEQSLLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRNQLQCHQCGHN
EPTPDHCPECGTFDHLVACGPGVERIAEEVEKHFPEARTIVLSSDLMGVKRLRLELEAIVKGEADIVIGTQLVAKGHNFP
LMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSHGLLQTYQPQHPVMQAIVSGDASAFYEREIVEREKAV
LPPFGRLASIIVSADTRAEAETHARGLRAAAPQVTGIMLLGPAEAPLALIRGRHRFRLLVHGRRNSDMQSFLRTLLASGP
KERGSVHVQLDIDPQSFL
>Mature_737_residues
AKDSSDLFGALFDPPSPTRTVPVLVPLPAPGPYSYAVPEDMVVETGSVVQVPLGPRQVFGVVWDDAGEKGVDPKKLRPIT
KSFECPPLKVEMRRFVDWVAAYTLSPPGLVARMALRAPAAFDPEPMIEGLRLTEGRPERLTPARERVMELAAEGQGWTKS
GLAHAAGVSTSVIDGLVKQGVFETVFLPAPPVVAEPDPDYVEPRLEGPQKQAASEIIEDVRKGGFHVSLIDGVTGSGKTE
VYFEAVAETLRQGKQVLILLPEIALTAAFLERFQDRFGAKPAEWHSDLSPRMREKVWRQAVTGEIKVVAGARSALFLPFD
NLGLIIVDEEHDPAYKQEDRVFYNARDMAVVRARIAEFPVVLVSATPSVESQVNGSSGRYNTIHLHTRFGDAAMPDLHLV
DMRRHPPERGGFLSPVLLRGIGKTIEKGEQSLLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRNQLQCHQCGHNE
PTPDHCPECGTFDHLVACGPGVERIAEEVEKHFPEARTIVLSSDLMGVKRLRLELEAIVKGEADIVIGTQLVAKGHNFPL
MTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSHGLLQTYQPQHPVMQAIVSGDASAFYEREIVEREKAVL
PPFGRLASIIVSADTRAEAETHARGLRAAAPQVTGIMLLGPAEAPLALIRGRHRFRLLVHGRRNSDMQSFLRTLLASGPK
ERGSVHVQLDIDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=736, Percent_Identity=33.2880434782609, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 80947; Mature: 80816

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDSSDLFGALFDPPSPTRTVPVLVPLPAPGPYSYAVPEDMVVETGSVVQVPLGPRQVF
CCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHEECCCCEEEECCCHHHEE
GVVWDDAGEKGVDPKKLRPITKSFECPPLKVEMRRFVDWVAAYTLSPPGLVARMALRAPA
EEEECCCCCCCCCHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
AFDPEPMIEGLRLTEGRPERLTPARERVMELAAEGQGWTKSGLAHAAGVSTSVIDGLVKQ
CCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHCCHHHCCCHHHHHHHHHHC
GVFETVFLPAPPVVAEPDPDYVEPRLEGPQKQAASEIIEDVRKGGFHVSLIDGVTGSGKT
CCHHEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
EVYFEAVAETLRQGKQVLILLPEIALTAAFLERFQDRFGAKPAEWHSDLSPRMREKVWRQ
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHH
AVTGEIKVVAGARSALFLPFDNLGLIIVDEEHDPAYKQEDRVFYNARDMAVVRARIAEFP
HHCCCEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHCC
VVLVSATPSVESQVNGSSGRYNTIHLHTRFGDAAMPDLHLVDMRRHPPERGGFLSPVLLR
EEEEECCCCHHHCCCCCCCCEEEEEEEEECCCCCCCCEEEEHHHCCCCCCCCCHHHHHHH
GIGKTIEKGEQSLLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRNQLQCHQCGHN
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCCC
EPTPDHCPECGTFDHLVACGPGVERIAEEVEKHFPEARTIVLSSDLMGVKRLRLELEAIV
CCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
KGEADIVIGTQLVAKGHNFPLMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTG
CCCCCEEEEHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
LKSHGLLQTYQPQHPVMQAIVSGDASAFYEREIVEREKAVLPPFGRLASIIVSADTRAEA
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
ETHARGLRAAAPQVTGIMLLGPAEAPLALIRGRHRFRLLVHGRRNSDMQSFLRTLLASGP
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
KERGSVHVQLDIDPQSFL
CCCCEEEEEEECCHHHCC
>Mature Secondary Structure 
AKDSSDLFGALFDPPSPTRTVPVLVPLPAPGPYSYAVPEDMVVETGSVVQVPLGPRQVF
CCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHEECCCCEEEECCCHHHEE
GVVWDDAGEKGVDPKKLRPITKSFECPPLKVEMRRFVDWVAAYTLSPPGLVARMALRAPA
EEEECCCCCCCCCHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
AFDPEPMIEGLRLTEGRPERLTPARERVMELAAEGQGWTKSGLAHAAGVSTSVIDGLVKQ
CCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHCCHHHCCCHHHHHHHHHHC
GVFETVFLPAPPVVAEPDPDYVEPRLEGPQKQAASEIIEDVRKGGFHVSLIDGVTGSGKT
CCHHEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
EVYFEAVAETLRQGKQVLILLPEIALTAAFLERFQDRFGAKPAEWHSDLSPRMREKVWRQ
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHH
AVTGEIKVVAGARSALFLPFDNLGLIIVDEEHDPAYKQEDRVFYNARDMAVVRARIAEFP
HHCCCEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHCC
VVLVSATPSVESQVNGSSGRYNTIHLHTRFGDAAMPDLHLVDMRRHPPERGGFLSPVLLR
EEEEECCCCHHHCCCCCCCCEEEEEEEEECCCCCCCCEEEEHHHCCCCCCCCCHHHHHHH
GIGKTIEKGEQSLLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRNQLQCHQCGHN
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCCC
EPTPDHCPECGTFDHLVACGPGVERIAEEVEKHFPEARTIVLSSDLMGVKRLRLELEAIV
CCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
KGEADIVIGTQLVAKGHNFPLMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTG
CCCCCEEEEHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
LKSHGLLQTYQPQHPVMQAIVSGDASAFYEREIVEREKAVLPPFGRLASIIVSADTRAEA
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
ETHARGLRAAAPQVTGIMLLGPAEAPLALIRGRHRFRLLVHGRRNSDMQSFLRTLLASGP
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
KERGSVHVQLDIDPQSFL
CCCCEEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]