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The map label for this gene is dacA [H]

Identifier: 159185157

GI number: 159185157

Start: 2298457

End: 2299752

Strand: Direct

Name: dacA [H]

Synonym: Atu2321

Alternate gene names: 159185157

Gene position: 2298457-2299752 (Clockwise)

Preceding gene: 15889595

Following gene: 15889608

Centisome position: 80.89

GC content: 62.89

Gene sequence:

>1296_bases
ATGTCGGGAATGGTCAAACGGGGAATGGCGGGTGCATGGGTCTTCGCGCTCACCTTCTTTTGCGCGATCCTCTCCTTGCA
GGCATCGCCTGCCCAGGCCGGCTACGCCCACTTCATCATGGATGCCAATACCGGCAAGGTTCTGGCCGCCCGCAATGCCG
ATGTGCTGAACCACCCCGCCTCGCTCACCAAGATGATGACGCTTTACATGACCTTCGAAGCGCTGCACGCCGGCCGGCTT
CGCTGGGACCAGAAGATCACCATGTCCAAAAACGGCGCATCGGTGATCCCCTCCAAGCTTTATGTGCGTCAGGGCCAGAC
CTTCACGGTGCGCGAAGCCGTTTATGGCATGATCGTCAAATCTGCCAATGACATGGCGGAAGGTATGGGCGATCACCTCG
GCGGTTCCGAGGCACGGTTCGCGGAAATGATGACCCGCAAGGCACGCCAGCTCGGCATGTCGAAGACGGTGTTCCGCAAT
GCCTCCGGCCTGCCCAGCAAATCCCAGGTGACGACGGCGCGCGACATGGCCAGGCTCGGCCTGGCGCTGCAGCGGGACTT
CCCCAAAGAATACGGCCTCTTCGCCATGGAATCCTTCAGTTTCCGGGGAAAACGCATCCGCGGCCATAACAATCTGATGT
ATCGATATCAGGGCATGGACGGCATCAAGACTGGCTACACCAATGCCTCTGGCTTCAACCTCGTCAGCGCCATCAACCAC
AATGGCCGCCGCGTCGTCGGCGTCGTGCTGGGTGGCAAGACCGCCCGCAGCCGTGACGCCCAGATGGCAGCCCTTCTCGA
CAAGGCCGTGCCGCAGGCATCCAGAAGCCGCAACACCGAACAGCTCGTGGCCAGCGCCAATGTCAGCCGCACCTTCGATG
TGGCCGCCGCCTTGCCCCCGGCTTCCGTTCCATTGCCGATGTTCGCCGAACGCCGCGCCGACCCGGTCGCCATGCAGATC
GCCACCGCCAATAGCCAGATGGCCGACATGATGCAGGTCTCCGCCATTCCGAGACCTGCTCCCGCCGCCGCTACGACCGG
TGAGCGAAGCCGCTGGGAAGTGCAGATCGCCGCGACCGACAGCGAAGCCGCCGCCCGCTCACTGCTCGCCAATGCCCGCT
CCAATATCGGCAGCTATACCGGGATCGCTCCCTACACCGAGGCCGTGCAAAGCGGATCGGCAACGCTTTACCGCGCCCGC
TTCACCGGCTTCGAAGACCAGTCTTCGGCCGTATCCGCCTGCAAGGAACTGAAGGCGCAATCCTATGCCTGCGTGGTGAT
GACCAGCGAGGGGTGA

Upstream 100 bases:

>100_bases
ATTAAGCATGAAAGCGATCTCTAAAGTTTTAGTGGTTTTGTTGCCGGAAAGTGTCAGTAAAACTCCGTCCATCGTGGCGG
TCGAACAAAAGGGGTGGATT

Downstream 100 bases:

>100_bases
TTCAGCGCTTGAGCCACCCGTTCGTCAACGCATCCGCCAGATACGGGATGCCGTTCACGCGCGCCATTTCAGACATGGCC
AGATTATCGTAGCGGATATT

Product: D-alanyl-D-alanine carboxypeptidase (penicillin binding protein)

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSGMVKRGMAGAWVFALTFFCAILSLQASPAQAGYAHFIMDANTGKVLAARNADVLNHPASLTKMMTLYMTFEALHAGRL
RWDQKITMSKNGASVIPSKLYVRQGQTFTVREAVYGMIVKSANDMAEGMGDHLGGSEARFAEMMTRKARQLGMSKTVFRN
ASGLPSKSQVTTARDMARLGLALQRDFPKEYGLFAMESFSFRGKRIRGHNNLMYRYQGMDGIKTGYTNASGFNLVSAINH
NGRRVVGVVLGGKTARSRDAQMAALLDKAVPQASRSRNTEQLVASANVSRTFDVAAALPPASVPLPMFAERRADPVAMQI
ATANSQMADMMQVSAIPRPAPAAATTGERSRWEVQIAATDSEAAARSLLANARSNIGSYTGIAPYTEAVQSGSATLYRAR
FTGFEDQSSAVSACKELKAQSYACVVMTSEG

Sequences:

>Translated_431_residues
MSGMVKRGMAGAWVFALTFFCAILSLQASPAQAGYAHFIMDANTGKVLAARNADVLNHPASLTKMMTLYMTFEALHAGRL
RWDQKITMSKNGASVIPSKLYVRQGQTFTVREAVYGMIVKSANDMAEGMGDHLGGSEARFAEMMTRKARQLGMSKTVFRN
ASGLPSKSQVTTARDMARLGLALQRDFPKEYGLFAMESFSFRGKRIRGHNNLMYRYQGMDGIKTGYTNASGFNLVSAINH
NGRRVVGVVLGGKTARSRDAQMAALLDKAVPQASRSRNTEQLVASANVSRTFDVAAALPPASVPLPMFAERRADPVAMQI
ATANSQMADMMQVSAIPRPAPAAATTGERSRWEVQIAATDSEAAARSLLANARSNIGSYTGIAPYTEAVQSGSATLYRAR
FTGFEDQSSAVSACKELKAQSYACVVMTSEG
>Mature_430_residues
SGMVKRGMAGAWVFALTFFCAILSLQASPAQAGYAHFIMDANTGKVLAARNADVLNHPASLTKMMTLYMTFEALHAGRLR
WDQKITMSKNGASVIPSKLYVRQGQTFTVREAVYGMIVKSANDMAEGMGDHLGGSEARFAEMMTRKARQLGMSKTVFRNA
SGLPSKSQVTTARDMARLGLALQRDFPKEYGLFAMESFSFRGKRIRGHNNLMYRYQGMDGIKTGYTNASGFNLVSAINHN
GRRVVGVVLGGKTARSRDAQMAALLDKAVPQASRSRNTEQLVASANVSRTFDVAAALPPASVPLPMFAERRADPVAMQIA
TANSQMADMMQVSAIPRPAPAAATTGERSRWEVQIAATDSEAAARSLLANARSNIGSYTGIAPYTEAVQSGSATLYRARF
TGFEDQSSAVSACKELKAQSYACVVMTSEG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=244, Percent_Identity=35.655737704918, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787062, Length=270, Percent_Identity=35.9259259259259, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87082022, Length=235, Percent_Identity=31.9148936170213, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI87082054, Length=249, Percent_Identity=29.3172690763052, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46430; Mature: 46299

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGMVKRGMAGAWVFALTFFCAILSLQASPAQAGYAHFIMDANTGKVLAARNADVLNHPA
CCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCHHHCCCH
SLTKMMTLYMTFEALHAGRLRWDQKITMSKNGASVIPSKLYVRQGQTFTVREAVYGMIVK
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHEEECCCEEEHHHHHHHHHHH
SANDMAEGMGDHLGGSEARFAEMMTRKARQLGMSKTVFRNASGLPSKSQVTTARDMARLG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
LALQRDFPKEYGLFAMESFSFRGKRIRGHNNLMYRYQGMDGIKTGYTNASGFNLVSAINH
HHHHHCCCHHHCCEEEECCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHCC
NGRRVVGVVLGGKTARSRDAQMAALLDKAVPQASRSRNTEQLVASANVSRTFDVAAALPP
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCEEHHHCCCC
ASVPLPMFAERRADPVAMQIATANSQMADMMQVSAIPRPAPAAATTGERSRWEVQIAATD
CCCCCCHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECC
SEAAARSLLANARSNIGSYTGIAPYTEAVQSGSATLYRARFTGFEDQSSAVSACKELKAQ
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCC
SYACVVMTSEG
CEEEEEEECCC
>Mature Secondary Structure 
SGMVKRGMAGAWVFALTFFCAILSLQASPAQAGYAHFIMDANTGKVLAARNADVLNHPA
CCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCHHHCCCH
SLTKMMTLYMTFEALHAGRLRWDQKITMSKNGASVIPSKLYVRQGQTFTVREAVYGMIVK
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHEEECCCEEEHHHHHHHHHHH
SANDMAEGMGDHLGGSEARFAEMMTRKARQLGMSKTVFRNASGLPSKSQVTTARDMARLG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
LALQRDFPKEYGLFAMESFSFRGKRIRGHNNLMYRYQGMDGIKTGYTNASGFNLVSAINH
HHHHHCCCHHHCCEEEECCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHCC
NGRRVVGVVLGGKTARSRDAQMAALLDKAVPQASRSRNTEQLVASANVSRTFDVAAALPP
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCEEHHHCCCC
ASVPLPMFAERRADPVAMQIATANSQMADMMQVSAIPRPAPAAATTGERSRWEVQIAATD
CCCCCCHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECC
SEAAARSLLANARSNIGSYTGIAPYTEAVQSGSATLYRARFTGFEDQSSAVSACKELKAQ
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCC
SYACVVMTSEG
CEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]