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The map label for this gene is murE

Identifier: 159185046

GI number: 159185046

Start: 2063709

End: 2065178

Strand: Reverse

Name: murE

Synonym: Atu2099

Alternate gene names: 159185046

Gene position: 2065178-2063709 (Counterclockwise)

Preceding gene: 15889384

Following gene: 15889382

Centisome position: 72.68

GC content: 61.16

Gene sequence:

>1470_bases
ATGAATTTGCGAGATATATCCGGAAATGCGTTTCCGGAATTGAAAGAATTGCTCCTGTCTGAAATCGGGGCGATCGAAAT
CGGGGGGATCACCGCAGACAGCCGCAAGGCTGCGCCCGGCAGCCTGTTCGTCGCGGTTGCAGGCACCAAGGCTGACGGTG
CGGCCTATGTGAAGGACGCGGTTGCCAAAGGTGCGGTCGCCGTTGTTTCCGGACACGCAGTGGAAGCCGACGTGCCGGTG
CTGGTGGTCACGGACCCGCGCCTTTATCTCTCGCTTGCCGCCTCCCGTTTTTACGGAAAACAACCTGATACCATGGTTGC
CGTCACCGGCACGGCGGGCAAAACATCCGTTGCCTCCTTCGTGCGGCAGATCTGGGCTTTTGCTGGCCATGCCGCCGCTC
AGATCGGCACCACGGGCGTCATCGCTCCTGGTCGCGAAGATTATGGCGCGCTGACGACGCCCGATCCGGTGACGCTGCAC
GCGCTTCTGGCCGAACTTGCGAGCGAAGGCGTGACCCATGCGGCAATGGAGGCCTCCAGCCATGGTCTGGACCAGCGTCG
TCTTGATGGCGTGCACCTTTCCGCCGCCGGCTTCACCAATCTCGGCCGCGATCACATGGATTACCATCCGACCATCGAGG
ACTATATGGCCGCGAAGATGCGGCTCTTCGATACGCTGATGGAAAAAGGCGCTCCCGCCGTGATCTTTGCTGACGATCCA
TGGTCGGACAAGGCGATCGGTGCGGCGCGCGAAGCGGGTCTCGAGGTGCGCACCGTCGGCCGCAACGGTCAATATCTGAC
GCTGAAGCGTGTCGAGCATTTCCGTCACAAGCAGATGATCGAAGTACATCATGACGGCGTAATCTTTGAAGTCGACATTC
CATTGGCTGGCGATTTCCAGGTGGCGAATGCGCTTGTCGCTGCCGGTCTTGCCATGTCCACCGGCGTTCCCGCCGCGACA
GCGTTGAAGGCGCTTGAAAAGCTTGTTGGCGCTGCCGGTCGTCTCGAACTCGTCGGCCAGACGAAGAATGGCGCGCTCGC
CTATGTGGATTACGCCCACAAGCCCGACGCACTGGAAAACGTGCTGACCTCGGTTAGGCCCTTCACCTCCGGCCGAATCA
TTACCGTTTTCGGTTGTGGTGGCGACCGCGACAAGGGCAAGCGCCCGATCATGGGCGAGGTGGCGACGCGGCTTTCCGAC
ATCGTCATCGTCACGGACGACAATCCGCGTTCGGAAGATGCCGCAACGATCCGCTCCGAGGTCATGGCGGCGGCAGCCGG
CGCGTTGGAAATCGGCGATCGGGCAGAGGCGATCCGTCATGCGGTGTCGATGCTGTCGCATGGCGATACGCTGATCGTCG
CGGGCAAGGGGCACGAAGAAGGGCAGATCGTCGGCTCCGTGACGCTGCCGTTTTCCGATCATGAACAGGTGCGCTCTGCA
CTGGCAGAGCTGGAAGGATCGAAGATTTGA

Upstream 100 bases:

>100_bases
GAAGTTCGGCAAGGATGGCTCGGCCTTGCTCGTGTCTTATTGATTGTTAGCTTATGATGTTCCGGCGATTCGCGCCGGGG
GGACAAGACGGACACAGTCG

Downstream 100 bases:

>100_bases
GCTGGTTATGGACAGTCGCCGACATGATCGCCATCACGGCAGGCCGCCCGGTGGGCAACCTGCCGACCGGTATCACCGGC
ATTTCCATCGACAGCCGGAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MNLRDISGNAFPELKELLLSEIGAIEIGGITADSRKAAPGSLFVAVAGTKADGAAYVKDAVAKGAVAVVSGHAVEADVPV
LVVTDPRLYLSLAASRFYGKQPDTMVAVTGTAGKTSVASFVRQIWAFAGHAAAQIGTTGVIAPGREDYGALTTPDPVTLH
ALLAELASEGVTHAAMEASSHGLDQRRLDGVHLSAAGFTNLGRDHMDYHPTIEDYMAAKMRLFDTLMEKGAPAVIFADDP
WSDKAIGAAREAGLEVRTVGRNGQYLTLKRVEHFRHKQMIEVHHDGVIFEVDIPLAGDFQVANALVAAGLAMSTGVPAAT
ALKALEKLVGAAGRLELVGQTKNGALAYVDYAHKPDALENVLTSVRPFTSGRIITVFGCGGDRDKGKRPIMGEVATRLSD
IVIVTDDNPRSEDAATIRSEVMAAAAGALEIGDRAEAIRHAVSMLSHGDTLIVAGKGHEEGQIVGSVTLPFSDHEQVRSA
LAELEGSKI

Sequences:

>Translated_489_residues
MNLRDISGNAFPELKELLLSEIGAIEIGGITADSRKAAPGSLFVAVAGTKADGAAYVKDAVAKGAVAVVSGHAVEADVPV
LVVTDPRLYLSLAASRFYGKQPDTMVAVTGTAGKTSVASFVRQIWAFAGHAAAQIGTTGVIAPGREDYGALTTPDPVTLH
ALLAELASEGVTHAAMEASSHGLDQRRLDGVHLSAAGFTNLGRDHMDYHPTIEDYMAAKMRLFDTLMEKGAPAVIFADDP
WSDKAIGAAREAGLEVRTVGRNGQYLTLKRVEHFRHKQMIEVHHDGVIFEVDIPLAGDFQVANALVAAGLAMSTGVPAAT
ALKALEKLVGAAGRLELVGQTKNGALAYVDYAHKPDALENVLTSVRPFTSGRIITVFGCGGDRDKGKRPIMGEVATRLSD
IVIVTDDNPRSEDAATIRSEVMAAAAGALEIGDRAEAIRHAVSMLSHGDTLIVAGKGHEEGQIVGSVTLPFSDHEQVRSA
LAELEGSKI
>Mature_489_residues
MNLRDISGNAFPELKELLLSEIGAIEIGGITADSRKAAPGSLFVAVAGTKADGAAYVKDAVAKGAVAVVSGHAVEADVPV
LVVTDPRLYLSLAASRFYGKQPDTMVAVTGTAGKTSVASFVRQIWAFAGHAAAQIGTTGVIAPGREDYGALTTPDPVTLH
ALLAELASEGVTHAAMEASSHGLDQRRLDGVHLSAAGFTNLGRDHMDYHPTIEDYMAAKMRLFDTLMEKGAPAVIFADDP
WSDKAIGAAREAGLEVRTVGRNGQYLTLKRVEHFRHKQMIEVHHDGVIFEVDIPLAGDFQVANALVAAGLAMSTGVPAAT
ALKALEKLVGAAGRLELVGQTKNGALAYVDYAHKPDALENVLTSVRPFTSGRIITVFGCGGDRDKGKRPIMGEVATRLSD
IVIVTDDNPRSEDAATIRSEVMAAAAGALEIGDRAEAIRHAVSMLSHGDTLIVAGKGHEEGQIVGSVTLPFSDHEQVRSA
LAELEGSKI

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=496, Percent_Identity=37.7016129032258, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1786274, Length=122, Percent_Identity=35.2459016393443, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_AGRT5 (Q8UDM3)

Other databases:

- EMBL:   AE007869
- PIR:   AD2834
- PIR:   H97611
- RefSeq:   NP_355064.2
- ProteinModelPortal:   Q8UDM3
- SMR:   Q8UDM3
- STRING:   Q8UDM3
- GeneID:   1134137
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2099
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- PhylomeDB:   Q8UDM3
- ProtClustDB:   PRK00139
- BioCyc:   ATUM176299-1:ATU2099-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 51288; Mature: 51288

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: BINDING 34-34 BINDING 179-179 BINDING 185-185 BINDING 187-187 BINDING 383-383 BINDING 455-455 BINDING 459-459

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRDISGNAFPELKELLLSEIGAIEIGGITADSRKAAPGSLFVAVAGTKADGAAYVKDA
CCCCCCCCCCCHHHHHHHHHHCCCEEECCEECCCCCCCCCEEEEEEECCCCCCHHHHHHH
VAKGAVAVVSGHAVEADVPVLVVTDPRLYLSLAASRFYGKQPDTMVAVTGTAGKTSVASF
HHCCCEEEEECCEEECCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
VRQIWAFAGHAAAQIGTTGVIAPGREDYGALTTPDPVTLHALLAELASEGVTHAAMEASS
HHHHHHHHCCHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
HGLDQRRLDGVHLSAAGFTNLGRDHMDYHPTIEDYMAAKMRLFDTLMEKGAPAVIFADDP
CCCCHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
WSDKAIGAAREAGLEVRTVGRNGQYLTLKRVEHFRHKQMIEVHHDGVIFEVDIPLAGDFQ
CCCCHHCHHHHCCCEEEEECCCCCEEEHHHHHHHHHHHHEEEECCCEEEEEECCCCCCHH
VANALVAAGLAMSTGVPAATALKALEKLVGAAGRLELVGQTKNGALAYVDYAHKPDALEN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCHHHHH
VLTSVRPFTSGRIITVFGCGGDRDKGKRPIMGEVATRLSDIVIVTDDNPRSEDAATIRSE
HHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
VMAAAAGALEIGDRAEAIRHAVSMLSHGDTLIVAGKGHEEGQIVGSVTLPFSDHEQVRSA
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHH
LAELEGSKI
HHHCCCCCC
>Mature Secondary Structure
MNLRDISGNAFPELKELLLSEIGAIEIGGITADSRKAAPGSLFVAVAGTKADGAAYVKDA
CCCCCCCCCCCHHHHHHHHHHCCCEEECCEECCCCCCCCCEEEEEEECCCCCCHHHHHHH
VAKGAVAVVSGHAVEADVPVLVVTDPRLYLSLAASRFYGKQPDTMVAVTGTAGKTSVASF
HHCCCEEEEECCEEECCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
VRQIWAFAGHAAAQIGTTGVIAPGREDYGALTTPDPVTLHALLAELASEGVTHAAMEASS
HHHHHHHHCCHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
HGLDQRRLDGVHLSAAGFTNLGRDHMDYHPTIEDYMAAKMRLFDTLMEKGAPAVIFADDP
CCCCHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
WSDKAIGAAREAGLEVRTVGRNGQYLTLKRVEHFRHKQMIEVHHDGVIFEVDIPLAGDFQ
CCCCHHCHHHHCCCEEEEECCCCCEEEHHHHHHHHHHHHEEEECCCEEEEEECCCCCCHH
VANALVAAGLAMSTGVPAATALKALEKLVGAAGRLELVGQTKNGALAYVDYAHKPDALEN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCHHHHH
VLTSVRPFTSGRIITVFGCGGDRDKGKRPIMGEVATRLSDIVIVTDDNPRSEDAATIRSE
HHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
VMAAAAGALEIGDRAEAIRHAVSMLSHGDTLIVAGKGHEEGQIVGSVTLPFSDHEQVRSA
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHH
LAELEGSKI
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194