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The map label for this gene is ddl

Identifier: 159185044

GI number: 159185044

Start: 2051211

End: 2052137

Strand: Reverse

Name: ddl

Synonym: Atu2089

Alternate gene names: 159185044

Gene position: 2052137-2051211 (Counterclockwise)

Preceding gene: 159185045

Following gene: 159185043

Centisome position: 72.22

GC content: 59.87

Gene sequence:

>927_bases
ATGGGCGGCAAGCACGTAGCTGTTCTTTTGGGTGGATTTTCGTCGGAGCGGCCGGTCAGTCTCTCGTCGGGCAACGCCTG
CGCGCTTGCGCTCGAAGGCGAAGGCTACAAGGTCACCCGTGTTGATGTGGGGCGCGATGTCGCCGCCGTACTCGATGAGC
TGCGCCCGGATGTCGCCTTCAACGCCCTGCATGGCCCCTTCGGCGAGGACGGCACCATTCAGGGTATCCTCGAATATCTG
GCCATTCCCTATACACATTCCGGCGTGCTGGCGTCCGCCCTTGCCATGGATAAGGCGCAGGCCAAGAAGGTTGCCGCCGC
CGCTGGCATCCCGGTTGCTGGCGAGCGCGTCATGAACCGCTTCGATTTCACCAGCGAACATCCGCTGCAGCCGCCCTATG
TGGTGAAGCCGGTGCGCGAAGGTTCGAGCTTCGGGGTCGTCATCGTCAAGGAAGACCAGTCGCATCCGCCGCAGATTCTC
ACCTCGTCGGAATGGCCTTTCGGCAACCAGGTGATGGTCGAGCGCTACATTCATGGGCGCGAACTGACCTGTGGCGTGCT
CGACGGCGAGGCGCTGGGCGTCACCGAAGTGGTGCCGCTGGGTCACAATTTCTATGATTATGATGCGAAATACGCTGCTG
GTGGCTCGAAACATGTCATTCCCGCAGGAATTTCACCGAAAATTTACCAAAAGATTCAAACATTGGCGGTTATGGCGCAT
CAGGCGATCGGGTGCCGTGGCGTAAGCCGTTCAGACTTTCGTTACGACGACCGCTTCTCAGAAGATGGCGAAGTTATCTG
GCTTGAAGTGAATACGCAGCCGGGCATGACGCCGACCTCCCTGGTGCCGGAAATGGCGGCCCATGCTGGCCGCTCCTTTG
GTGACCTTGTCAGCTGGATGGTGGAGGACGCTTCGTGTTTGCGGTGA

Upstream 100 bases:

>100_bases
CGTTTAACTGATCTCAAGGATCATGTGCCAGAATCACCGTCCGTTTCATTCGGTCGGTTTGGACATGGTTCGTAATGGAG
AGTTGGTGTCAGAGGGTTCG

Downstream 100 bases:

>100_bases
CCGGCAAAAAGTCGACGGCAAAAAAGCGCGAGCAATACGCGGCGACGGCCAATGCCGACGATCGCAGGGTGCTGCCGCGT
CCTCTGCGTCGCTTCGTGCG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MGGKHVAVLLGGFSSERPVSLSSGNACALALEGEGYKVTRVDVGRDVAAVLDELRPDVAFNALHGPFGEDGTIQGILEYL
AIPYTHSGVLASALAMDKAQAKKVAAAAGIPVAGERVMNRFDFTSEHPLQPPYVVKPVREGSSFGVVIVKEDQSHPPQIL
TSSEWPFGNQVMVERYIHGRELTCGVLDGEALGVTEVVPLGHNFYDYDAKYAAGGSKHVIPAGISPKIYQKIQTLAVMAH
QAIGCRGVSRSDFRYDDRFSEDGEVIWLEVNTQPGMTPTSLVPEMAAHAGRSFGDLVSWMVEDASCLR

Sequences:

>Translated_308_residues
MGGKHVAVLLGGFSSERPVSLSSGNACALALEGEGYKVTRVDVGRDVAAVLDELRPDVAFNALHGPFGEDGTIQGILEYL
AIPYTHSGVLASALAMDKAQAKKVAAAAGIPVAGERVMNRFDFTSEHPLQPPYVVKPVREGSSFGVVIVKEDQSHPPQIL
TSSEWPFGNQVMVERYIHGRELTCGVLDGEALGVTEVVPLGHNFYDYDAKYAAGGSKHVIPAGISPKIYQKIQTLAVMAH
QAIGCRGVSRSDFRYDDRFSEDGEVIWLEVNTQPGMTPTSLVPEMAAHAGRSFGDLVSWMVEDASCLR
>Mature_307_residues
GGKHVAVLLGGFSSERPVSLSSGNACALALEGEGYKVTRVDVGRDVAAVLDELRPDVAFNALHGPFGEDGTIQGILEYLA
IPYTHSGVLASALAMDKAQAKKVAAAAGIPVAGERVMNRFDFTSEHPLQPPYVVKPVREGSSFGVVIVKEDQSHPPQILT
SSEWPFGNQVMVERYIHGRELTCGVLDGEALGVTEVVPLGHNFYDYDAKYAAGGSKHVIPAGISPKIYQKIQTLAVMAHQ
AIGCRGVSRSDFRYDDRFSEDGEVIWLEVNTQPGMTPTSLVPEMAAHAGRSFGDLVSWMVEDASCLR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=309, Percent_Identity=38.8349514563107, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1786579, Length=256, Percent_Identity=37.109375, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_AGRT5 (Q8UDN3)

Other databases:

- EMBL:   AE007869
- PIR:   AB2833
- PIR:   F97610
- RefSeq:   NP_355054.2
- ProteinModelPortal:   Q8UDN3
- SMR:   Q8UDN3
- STRING:   Q8UDN3
- GeneID:   1134127
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2089
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- PhylomeDB:   Q8UDN3
- ProtClustDB:   PRK01372
- BioCyc:   ATUM176299-1:ATU2089-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33069; Mature: 32938

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGKHVAVLLGGFSSERPVSLSSGNACALALEGEGYKVTRVDVGRDVAAVLDELRPDVAF
CCCCEEEEEEECCCCCCCEEECCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHCCCCEE
NALHGPFGEDGTIQGILEYLAIPYTHSGVLASALAMDKAQAKKVAAAAGIPVAGERVMNR
HHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FDFTSEHPLQPPYVVKPVREGSSFGVVIVKEDQSHPPQILTSSEWPFGNQVMVERYIHGR
HCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHCCC
ELTCGVLDGEALGVTEVVPLGHNFYDYDAKYAAGGSKHVIPAGISPKIYQKIQTLAVMAH
CEEEEEECCCCCCCEEEEECCCCEECCCCEECCCCCCEEEECCCCHHHHHHHHHHHHHHH
QAIGCRGVSRSDFRYDDRFSEDGEVIWLEVNTQPGMTPTSLVPEMAAHAGRSFGDLVSWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
VEDASCLR
HHHHHHCC
>Mature Secondary Structure 
GGKHVAVLLGGFSSERPVSLSSGNACALALEGEGYKVTRVDVGRDVAAVLDELRPDVAF
CCCEEEEEEECCCCCCCEEECCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHCCCCEE
NALHGPFGEDGTIQGILEYLAIPYTHSGVLASALAMDKAQAKKVAAAAGIPVAGERVMNR
HHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FDFTSEHPLQPPYVVKPVREGSSFGVVIVKEDQSHPPQILTSSEWPFGNQVMVERYIHGR
HCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHCCC
ELTCGVLDGEALGVTEVVPLGHNFYDYDAKYAAGGSKHVIPAGISPKIYQKIQTLAVMAH
CEEEEEECCCCCCCEEEEECCCCEECCCCEECCCCCCEEEECCCCHHHHHHHHHHHHHHH
QAIGCRGVSRSDFRYDDRFSEDGEVIWLEVNTQPGMTPTSLVPEMAAHAGRSFGDLVSWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
VEDASCLR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194